LOCUS       sequence-353213-       10941 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  mCherry fused to the C-terminus of XRCC1.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10941)
  AUTHORS   Wilk A, Hayat F, Cunningham R, Li J, Garavaglia S, Zamani L, 
            Ferraris DM, Sykora P, Andrews J, Clark J, Davis A, Chaloin L, Rizzi
            M, Migaud M, Sobol RW
  TITLE     Extracellular NAD(+) enhances PARP-dependent DNA repair capacity 
            independently of CD73 activity.
  JOURNAL   Sci Rep. 2020 Jan 20;10(1):651. doi: 10.1038/s41598-020-57506-9.
  PUBMED    31959836
REFERENCE   2  (bases 1 to 10941)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Sci Rep."; 
            date: "2020-01-20"; volume: "10(1):651. doi"; pages: " 
            10.1038/s41598-020-57506-9"
COMMENT     Sequence Label: pLV-CMV-hXRCC1-mCherry-Hygro
FEATURES             Location/Qualifiers
     source          1..10941
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        3..229
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             230..410
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    457..582
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1075..1308
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1493..1537
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    1803..1920
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     protein_bind    1938..1958
                     /gene="mutant version of attB"
                     /label=attB4
                     /bound_moiety="BP Clonase(TM)"
                     /note="core recombination site for the Gateway(R) BP 
                     reaction"
     enhancer        2019..2322
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        2323..2526
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     2477..2497
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     protein_bind    2547..2571
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             4477..5187
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     complement(4622..4640)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     4890..4909
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     protein_bind    complement(5188..5212)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     misc_feature    5230..5818
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(5283..5303)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(5701..5712)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     promoter        5849..6348
                     /label=PGK promoter
                     /note="mouse phosphoglycerate kinase 1 promoter"
     primer_bind     complement(5884..5907)
                     /label=MSCV-rev
                     /note="Murine stem cell virus, reverse primer"
     primer_bind     6281..6300
                     /label=mPGK-F
                     /note="Mouse PGK promoter, forward primer"
     CDS             6365..7390
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     E"
     CDS             6365..7390
                     /codon_start=1
                     /gene="aph(4)-Ia"
                     /product="aminoglycoside phosphotransferase from E. coli"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     E"
     LTR             7462..7695
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    7767..7888
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(7804..7823)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     7858..7877
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      7928..8063
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(8083..8102)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(8084..8102)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(8112..8129)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(8112..8128)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(8121..8143)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      8270..8725
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(8357..8376)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     8567..8588
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        8751..8855
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(8856..8924,8925..9716)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(9074..9093)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      9887..10475
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     10376..10395
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     10629..10646
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    10763..10784
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(10799..10804,10805..10822,10823..10829)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    10837..10853
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     10842..10864
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     10861..10877
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     10861..10877
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     10896..10916
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        10898..10916
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
ORIGIN
        1 aatgtagtct tatgcaatac tcttgtagtc ttgcaacatg gtaacgatga gttagcaaca
       61 tgccttacaa ggagagaaaa agcaccgtgc atgccgattg gtggaagtaa ggtggtacga
      121 tcgtgcctta ttaggaaggc aacagacggg tctgacatgg attggacgaa ccactgaatt
      181 gccgcattgc agagatattg tatttaagtg cctagctcga tacataaacg ggtctctctg
      241 gttagaccag atctgagcct gggagctctc tggctaacta gggaacccac tgcttaagcc
      301 tcaataaagc ttgccttgag tgcttcaagt agtgtgtgcc cgtctgttgt gtgactctgg
      361 taactagaga tccctcagac ccttttagtc agtgtggaaa atctctagca gtggcgcccg
      421 aacagggact tgaaagcgaa agggaaacca gaggagctct ctcgacgcag gactcggctt
      481 gctgaagcgc gcacggcaag aggcgagggg cggcgactgg tgagtacgcc aaaaattttg
      541 actagcggag gctagaagga gagagatggg tgcgagagcg tcagtattaa gcgggggaga
      601 attagatcgc gatgggaaaa aattcggtta aggccagggg gaaagaaaaa atataaatta
      661 aaacatatag tatgggcaag cagggagcta gaacgattcg cagttaatcc tggcctgtta
      721 gaaacatcag aaggctgtag acaaatactg ggacagctac aaccatccct tcagacagga
      781 tcagaagaac ttagatcatt atataataca gtagcaaccc tctattgtgt gcatcaaagg
      841 atagagataa aagacaccaa ggaagcttta gacaagatag aggaagagca aaacaaaagt
      901 aagaccaccg cacagcaagc ggccgctgat cttcagacct ggaggaggag atatgaggga
      961 caattggaga agtgaattat ataaatataa agtagtaaaa attgaaccat taggagtagc
     1021 acccaccaag gcaaagagaa gagtggtgca gagagaaaaa agagcagtgg gaataggagc
     1081 tttgttcctt gggttcttgg gagcagcagg aagcactatg ggcgcagcgt caatgacgct
     1141 gacggtacag gccagacaat tattgtctgg tatagtgcag cagcagaaca atttgctgag
     1201 ggctattgag gcgcaacagc atctgttgca actcacagtc tggggcatca agcagctcca
     1261 ggcaagaatc ctggctgtgg aaagatacct aaaggatcaa cagctcctgg ggatttgggg
     1321 ttgctctgga aaactcattt gcaccactgc tgtgccttgg aatgctagtt ggagtaataa
     1381 atctctggaa cagatttgga atcacacgac ctggatggag tgggacagag aaattaacaa
     1441 ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga
     1501 acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa
     1561 ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat
     1621 agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt
     1681 tcagacccac ctcccaaccc cgaggggacc cgacaggccc gaaggaatag aagaagaagg
     1741 tggagagaga gacagagaca gatccattcg attagtgaac ggatctcgac ggtatcgcta
     1801 gcttttaaaa gaaaaggggg gattgggggg tacagtgcag gggaaagaat agtagacata
     1861 atagcaacag acatacaaac taaagaatta caaaaacaaa ttacaaaaat tcaaaatttt
     1921 actagtgatt atcggatcaa ctttgtatag aaaagttgta gttattaata gtaatcaatt
     1981 acggggtcat tagttcatag cccatatatg gagttccgcg ttacataact tacggtaaat
     2041 ggcccgcctg gctgaccgcc caacgacccc cgcccattga cgtcaataat gacgtatgtt
     2101 cccatagtaa cgccaatagg gactttccat tgacgtcaat gggtggagta tttacggtaa
     2161 actgcccact tggcagtaca tcaagtgtat catatgccaa gtacgccccc tattgacgtc
     2221 aatgacggta aatggcccgc ctggcattat gcccagtaca tgaccttatg ggactttcct
     2281 acttggcagt acatctacgt attagtcatc gctattacca tggtgatgcg gttttggcag
     2341 tacatcaatg ggcgtggata gcggtttgac tcacggggat ttccaagtct ccaccccatt
     2401 gacgtcaatg ggagtttgtt ttggcaccaa aatcaacggg actttccaaa atgtcgtaac
     2461 aactccgccc cattgacgca aatgggcggt aggcgtgtac ggtgggaggt ctatataagc
     2521 agagctggtt tagtgaaccg tcagatccaa gtttgtacaa aaaagcaggc tgccaccatg
     2581 ccggagatcc gcctccgcca tgtcgtgtcc tgcagcagcc aggactcgac tcactgtgca
     2641 gaaaatcttc tcaaggcaga cacttaccga aaatggcggg cagccaaggc aggcgagaag
     2701 accatctctg tggtcctaca gttggagaag gaggagcaga tacacagtgt ggacattggg
     2761 aatgatggct cagctttcgt ggaggtgctg gtgggcagtt cagctggagg cgctggggag
     2821 caagactatg aggtccttct ggtcacctca tctttcatgt ccccttccga gagccgcagt
     2881 ggctcaaacc ccaaccgcgt tcgcatgttt gggcctgaca agctggtccg ggcagccgcc
     2941 gagaagcgct gggaccgggt caaaattgtt tgcagccagc cctacagcaa ggactccccc
     3001 tttggcttga gttttgtacg gtttcatagc cccccagaca aagatgaggc agaggccccg
     3061 tcccagaagg tgacagtgac caagcttggc cagttccgtg tgaaggagga ggatgagagc
     3121 gccaactctc tgaggccggg ggctctcttc ttcagccgga tcaacaagac atccccagtc
     3181 acagccagcg acccagcagg acctagctat gcagctgcta ccctccaggc ttctagtgct
     3241 gcctcctcag cctctccagt ctccagggcc ataggcagca cctccaagcc ccaggagtct
     3301 cccaaaggga agaggaagtt ggatttgaac caagaagaaa agaagacccc cagcaaacca
     3361 ccagcccagc tgtcgccatc tgttcccaag agacctaaat tgccagctcc aactcgtacc
     3421 ccagccacag ccccagtccc tgcccgagca cagggggcag tgacaggcaa accccgagga
     3481 gaaggcaccg agcccagacg accccgagct ggcccagagg agctggggaa gatccttcag
     3541 ggtgtggtag tggtgctgag tggcttccag aaccccttcc gctccgagct gcgagataag
     3601 gccctagagc ttggggccaa gtatcggcca gactggaccc gggacagcac gcacctcatc
     3661 tgtgcctttg ccaacacccc caagtacagc caggtcctag gcctgggagg ccgcatcgtg
     3721 cgtaaggagt gggtgctgga ctgtcaccgc atgcgtcggc ggctgccctc ccagaggtac
     3781 ctcatggcag ggccaggttc cagcagtgag gaggatgagg cctctcacag cggtggcagc
     3841 ggagatgaag cccccaagct tcctcagaag caaccccaga ccaaaaccaa gcccactcag
     3901 gcagctggac ccagctcacc ccagaagccc ccaacccctg aagagaccaa agcagcctca
     3961 ccagtgctcc aggaagatat agacattgag ggggtacagt cagaaggaca ggacaatggg
     4021 gcggaagatt ctggggacac agaggatgag ctgaggaggg tggcagagca gaaggaacac
     4081 agactgcccc ctggccagga ggagaatggg gaagacccgt atgcaggctc cacggatgag
     4141 aacacggaca gtgaggaaca ccaggagcct cctgatctgc cagtccctga gctcccagat
     4201 ttcttccagg gcaagcactt ctttctttac ggggagttcc ctggggacga gcggcggaaa
     4261 ctcatccgat acgtcacagc cttcaatggg gagctcgagg actatatgag tgaccgggtt
     4321 cagtttgtga tcacagcaca ggaatgggat cccagctttg aggaggccct gatggacaac
     4381 ccctccctgg cattcgttcg tccccgatgg atctacagtt gcaatgagaa gcagaagtta
     4441 cttcctcacc agctctatgg ggtggtgccg caagccatgg tgagcaaggg cgaggaggat
     4501 aacatggcca tcatcaagga gttcatgcgc ttcaaggtgc acatggaggg ctccgtgaac
     4561 ggccacgagt tcgagatcga gggcgagggc gagggccgcc cctacgaggg cacccagacc
     4621 gccaagctga aggtgaccaa gggtggcccc ctgcccttcg cctgggacat cctgtcccct
     4681 cagttcatgt acggctccaa ggcctacgtg aagcaccccg ccgacatccc cgactacttg
     4741 aagctgtcct tccccgaggg cttcaagtgg gagcgcgtga tgaacttcga ggacggcggc
     4801 gtggtgaccg tgacccagga ctcctccctg caggacggcg agttcatcta caaggtgaag
     4861 ctgcgcggca ccaacttccc ctccgacggc cccgtaatgc agaagaagac catgggctgg
     4921 gaggcctcct ccgagcggat gtaccccgag gacggcgccc tgaagggcga gatcaagcag
     4981 aggctgaagc tgaaggacgg cggccactac gacgctgagg tcaagaccac ctacaaggcc
     5041 aagaagcccg tgcagctgcc cggcgcctac aacgtcaaca tcaagttgga catcacctcc
     5101 cacaacgagg actacaccat cgtggaacag tacgaacgcg ccgagggccg ccactccacc
     5161 ggcggcatgg acgagctgta caagtaaacc cagctttctt gtacaaagtg gtgataatcg
     5221 aattccgata atcaacctct ggattacaaa atttgtgaaa gattgactgg tattcttaac
     5281 tatgttgctc cttttacgct atgtggatac gctgctttaa tgcctttgta tcatgctatt
     5341 gcttcccgta tggctttcat tttctcctcc ttgtataaat cctggttgct gtctctttat
     5401 gaggagttgt ggcccgttgt caggcaacgt ggcgtggtgt gcactgtgtt tgctgacgca
     5461 acccccactg gttggggcat tgccaccacc tgtcagctcc tttccgggac tttcgctttc
     5521 cccctcccta ttgccacggc ggaactcatc gccgcctgcc ttgcccgctg ctggacaggg
     5581 gctcggctgt tgggcactga caattccgtg gtgttgtcgg ggaagctgac gtcctttcca
     5641 tggctgctcg cctgtgttgc cacctggatt ctgcgcggga cgtccttctg ctacgtccct
     5701 tcggccctca atccagcgga ccttccttcc cgcggcctgc tgccggctct gcggcctctt
     5761 ccgcgtcttc gccttcgccc tcagacgagt cggatctccc tttgggccgc ctccccgcat
     5821 cgggaattcc cgcggttcga attctaccgg gtaggggagg cgcttttccc aaggcagtct
     5881 ggagcatgcg ctttagcagc cccgctgggc acttggcgct acacaagtgg cctctggcct
     5941 cgcacacatt ccacatccac cggtaggcgc caaccggctc cgttctttgg tggccccttc
     6001 gcgccacctt ctactcctcc cctagtcagg aagttccccc ccgccccgca gctcgcgtcg
     6061 tgcaggacgt gacaaatgga agtagcacgt ctcactagtc tcgtgcagat ggacagcacc
     6121 gctgagcaat ggaagcgggt aggcctttgg ggcagcggcc aatagcagct ttgctccttc
     6181 gctttctggg ctcagaggct gggaaggggt gggtccgggg gcgggctcag gggcgggctc
     6241 aggggcgggg cgggcgcccg aaggtcctcc ggaggcccgg cattctgcac gcttcaaaag
     6301 cgcacgtctg ccgcgctgtt ctcctcttcc tcatctccgg gcctttcgac ctcacgtggc
     6361 caccatgaaa aagcctgaac tcaccgcgac gtctgtcgag aagtttctga tcgaaaagtt
     6421 cgacagcgtc tccgacctga tgcagctctc ggagggcgaa gaatctcgtg ctttcagctt
     6481 cgatgtagga gggcgtggat atgtcctgcg ggtaaatagc tgcgccgatg gtttctacaa
     6541 agatcgttat gtttatcggc actttgcatc ggccgcgctc ccgattccgg aagtgcttga
     6601 cattggggaa tttagcgaga gcctgaccta ttgcatctcc cgccgtgcac agggtgtcac
     6661 gttgcaagac ctgcctgaaa ccgaactgcc cgctgttctg cagccggtcg cggaggccat
     6721 ggatgcgatc gctgcggccg atcttagcca gacgagcggg ttcggcccat tcggaccgca
     6781 aggaatcggt caatacacta catggcgtga tttcatatgc gcgattgctg atccccatgt
     6841 gtatcactgg caaactgtga tggacgacac cgtcagtgcg tccgtcgcgc aggctctcga
     6901 tgagctgatg ctttgggccg aggactgccc cgaagtccgg cacctcgtgc acgcggattt
     6961 cggctccaac aatgtcctga cggacaatgg ccgcataaca gcggtcattg actggagcga
     7021 ggcgatgttc ggggattccc aatacgaggt cgccaacatc ttcttctgga ggccgtggtt
     7081 ggcttgtatg gagcagcaga cgcgctactt cgagcggagg catccggagc ttgcaggatc
     7141 gccgcggctc cgggcgtata tgctccgcat tggtcttgac caactctatc agagcttggt
     7201 tgacggcaat ttcgatgatg cagcttgggc gcagggtcga tgcgacgcaa tcgtccgatc
     7261 cggagccggg actgtcgggc gtacacaaat cgcccgcaga agcgcggccg tctggaccga
     7321 tggctgtgta gaagtactcg ccgatagtgg aaaccgacgc cccagcactc gtccgagggc
     7381 aaaggaatag ggtaccttta agaccaatga cttacaaggc agctgtagat cttagccact
     7441 ttttaaaaga aaagggggga ctggaagggc taattcactc ccaacgaaga caagatctgc
     7501 tttttgcttg tactgggtct ctctggttag accagatctg agcctgggag ctctctggct
     7561 aactagggaa cccactgctt aagcctcaat aaagcttgcc ttgagtgctt caagtagtgt
     7621 gtgcccgtct gttgtgtgac tctggtaact agagatccct cagacccttt tagtcagtgt
     7681 ggaaaatctc tagcagtagt agttcatgtc atcttattat tcagtattta taacttgcaa
     7741 agaaatgaat atcagagagt gagaggaact tgtttattgc agcttataat ggttacaaat
     7801 aaagcaatag catcacaaat ttcacaaata aagcattttt ttcactgcat tctagttgtg
     7861 gtttgtccaa actcatcaat gtatcttatc atgtctggct ctagctatcc cgcccctaac
     7921 tccgcccatc ccgcccctaa ctccgcccag ttccgcccat tctccgcccc atggctgact
     7981 aatttttttt atttatgcag aggccgaggc cgcctcggcc tctgagctat tccagaagta
     8041 gtgaggaggc ttttttggag gcctagggac gtacccaatt cgccctatag tgagtcgtat
     8101 tacgcgcgct cactggccgt cgttttacaa cgtcgtgact gggaaaaccc tggcgttacc
     8161 caacttaatc gccttgcagc acatccccct ttcgccagct ggcgtaatag cgaagaggcc
     8221 cgcaccgatc gcccttccca acagttgcgc agcctgaatg gcgaatggga cgcgccctgt
     8281 agcggcgcat taagcgcggc gggtgtggtg gttacgcgca gcgtgaccgc tacacttgcc
     8341 agcgccctag cgcccgctcc tttcgctttc ttcccttcct ttctcgccac gttcgccggc
     8401 tttccccgtc aagctctaaa tcgggggctc cctttagggt tccgatttag tgctttacgg
     8461 cacctcgacc ccaaaaaact tgattagggt gatggttcac gtagtgggcc atcgccctga
     8521 tagacggttt ttcgcccttt gacgttggag tccacgttct ttaatagtgg actcttgttc
     8581 caaactggaa caacactcaa ccctatctcg gtctattctt ttgatttata agggattttg
     8641 ccgatttcgg cctattggtt aaaaaatgag ctgatttaac aaaaatttaa cgcgaatttt
     8701 aacaaaatat taacgcttac aatttaggtg gcacttttcg gggaaatgtg cgcggaaccc
     8761 ctatttgttt atttttctaa atacattcaa atatgtatcc gctcatgaga caataaccct
     8821 gataaatgct tcaataatat tgaaaaagga agagtatgag tattcaacat ttccgtgtcg
     8881 cccttattcc cttttttgcg gcattttgcc ttcctgtttt tgctcaccca gaaacgctgg
     8941 tgaaagtaaa agatgctgaa gatcagttgg gtgcacgagt gggttacatc gaactggatc
     9001 tcaacagcgg taagatcctt gagagttttc gccccgaaga acgttttcca atgatgagca
     9061 cttttaaagt tctgctatgt ggcgcggtat tatcccgtat tgacgccggg caagagcaac
     9121 tcggtcgccg catacactat tctcagaatg acttggttga gtactcacca gtcacagaaa
     9181 agcatcttac ggatggcatg acagtaagag aattatgcag tgctgccata accatgagtg
     9241 ataacactgc ggccaactta cttctgacaa cgatcggagg accgaaggag ctaaccgctt
     9301 ttttgcacaa catgggggat catgtaactc gccttgatcg ttgggaaccg gagctgaatg
     9361 aagccatacc aaacgacgag cgtgacacca cgatgcctgt agcaatggca acaacgttgc
     9421 gcaaactatt aactggcgaa ctacttactc tagcttcccg gcaacaatta atagactgga
     9481 tggaggcgga taaagttgca ggaccacttc tgcgctcggc ccttccggct ggctggttta
     9541 ttgctgataa atctggagcc ggtgagcgtg ggtctcgcgg tatcattgca gcactggggc
     9601 cagatggtaa gccctcccgt atcgtagtta tctacacgac ggggagtcag gcaactatgg
     9661 atgaacgaaa tagacagatc gctgagatag gtgcctcact gattaagcat tggtaactgt
     9721 cagaccaagt ttactcatat atactttaga ttgatttaaa acttcatttt taatttaaaa
     9781 ggatctaggt gaagatcctt tttgataatc tcatgaccaa aatcccttaa cgtgagtttt
     9841 cgttccactg agcgtcagac cccgtagaaa agatcaaagg atcttcttga gatccttttt
     9901 ttctgcgcgt aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt
     9961 tgccggatca agagctacca actctttttc cgaaggtaac tggcttcagc agagcgcaga
    10021 taccaaatac tgttcttcta gtgtagccgt agttaggcca ccacttcaag aactctgtag
    10081 caccgcctac atacctcgct ctgctaatcc tgttaccagt ggctgctgcc agtggcgata
    10141 agtcgtgtct taccgggttg gactcaagac gatagttacc ggataaggcg cagcggtcgg
    10201 gctgaacggg gggttcgtgc acacagccca gcttggagcg aacgacctac accgaactga
    10261 gatacctaca gcgtgagcta tgagaaagcg ccacgcttcc cgaagagaga aaggcggaca
    10321 ggtatccggt aagcggcagg gtcggaacag gagagcgcac gagggagctt ccagggggaa
    10381 acgcctggta tctttatagt cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt
    10441 tgtgatgctc gtcagggggg cggagcctat ggaaaaacgc cagcaacgcg gcctttttac
    10501 ggttcctggc cttttgctgg ccttttgctc acatgttctt tcctgcgtta tcccctgatt
    10561 ctgtggataa ccgtattacc gcctttgagt gagctgatac cgctcgccgc agccgaacga
    10621 ccgagcgcag cgagtcagtg agcgaggaag cggaagagcg cccaatacgc aaaccgcctc
    10681 tccccgcgcg ttggccgatt cattaatgca gctggcacga caggtttccc gactggaaag
    10741 cgggcagtga gcgcaacgca attaatgtga gttagctcac tcattaggca ccccaggctt
    10801 tacactttat gcttccggct cgtatgttgt gtggaattgt gagcggataa caatttcaca
    10861 caggaaacag ctatgaccat gattacgcca agcgcgcaat taaccctcac taaagggaac
    10921 aaaagctgga gctgcaagct t
//
