LOCUS       sequence-353215-        9064 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9064)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 9064)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF2584
FEATURES             Location/Qualifiers
     source          1..9064
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        1..1179
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          232..1170
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     1127..1147
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     regulatory      1190..1199
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     misc_feature    2002..2590
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(2055..2075)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(2473..2484)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             2662..2895
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    2973..3094
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(3010..3029)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     3064..3083
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      3134..3269
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(3289..3308)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(3290..3308)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(3318..3335)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(3318..3334)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(3327..3349)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      3476..3931
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(3563..3582)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     3773..3794
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        3957..4061
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(4062..4130,4131..4922)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(4280..4299)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      5093..5681
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     5582..5601
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     5835..5852
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    5969..5990
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(6005..6010,6011..6028,6029..6035)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6043..6059
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     6048..6070
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     6067..6083
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     6067..6083
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     6102..6122
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        6104..6122
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        6150..6376
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             6377..6557
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    6604..6729
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    7222..7455
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             7640..7684
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     promoter        7915..8125
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     promoter        7937..8133
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     rep_origin      7984..8119
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             8165..8767
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGAS"
     primer_bind     complement(8165..8184)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     8661..8681
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     misc_feature    8810..8927
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
ORIGIN
        1 ggctccggtg cccgtcagtg ggcagagcgc acatcgccca cagtccccga gaagttgggg
       61 ggaggggtcg gcaattgaac cggtgcctag agaaggtggc gcggggtaaa ctgggaaagt
      121 gatgtcgtgt actggctccg cctttttccc gagggtgggg gagaaccgta tataagtgca
      181 gtagtcgccg tgaacgttct ttttcgcaac gggtttgccg ccagaacaca ggtaagtgcc
      241 gtgtgtggtt cccgcgggcc tggcctcttt acgggttatg gcccttgcgt gccttgaatt
      301 acttccacct ggctgcagta cgtgattctt gatcccgagc ttcgggttgg aagtgggtgg
      361 gagagttcga ggccttgcgc ttaaggagcc ccttcgcctc gtgcttgagt tgaggcctgg
      421 cctgggcgct ggggccgccg cgtgcgaatc tggtggcacc ttcgcgcctg tctcgctgct
      481 ttcgataagt ctctagccat ttaaaatttt tgatgacctg ctgcgacgct ttttttctgg
      541 caagatagtc ttgtaaatgc gggccaagat ctgcacactg gtatttcggt ttttggggcc
      601 gcgggcggcg acggggcccg tgcgtcccag cgcacatgtt cggcgaggcg gggcctgcga
      661 gcgcggccac cgagaatcgg acgggggtag tctcaagctg gccggcctgc tctggtgcct
      721 ggcctcgcgc cgccgtgtat cgccccgccc tgggcggcaa ggctggcccg gtcggcacca
      781 gttgcgtgag cggaaagatg gccgcttccc ggccctgctg cagggagctc aaaatggagg
      841 acgcggcgct cgggagagcg ggcgggtgag tcacccacac aaaggaaaag ggcctttccg
      901 tcctcagccg tcgcttcatg tgactccacg gagtaccggg cgccgtccag gcacctcgat
      961 tagttctcga gcttttggag tacgtcgtct ttaggttggg gggaggggtt ttatgcgatg
     1021 gagtttcccc acactgagtg ggtggagact gaagttaggc cagcttggca cttgatgtaa
     1081 ttctccttgg aatttgccct ttttgagttt ggatcttggt tcattctcaa gcctcagaca
     1141 gtggttcaaa gtttttttct tccatttcag gtgtcgtgag gctagcaccg ccaccatggc
     1201 taccgctgaa tctcgcgctc tgcaattcgc tgaaggtgct gctttcccgg cttatcgtgc
     1261 tccgcatgct ggtggtgctc tgctcccacc gccatctccg gctgctgctc tcctgccggc
     1321 tccaccggct ggtccgggtc cggctacttt cgctggtttt ctgggtcgtg atccgggtcc
     1381 agctccacca ccgccggctt ctctcggttc tccggctccg ccgaagggtg ctgctgctcc
     1441 atctgcttct caacgtcgta aacgtacctc cttttctgct gagcaactcc aactcctcga
     1501 actggttttt cgtcgtactc gttatccgga tattcatctg cgtgaacgtc tcgctgctct
     1561 gactctcctg ccggaatctc gtattcaagt ttggtttcaa aatcgtcgcg ctaaatcccg
     1621 tcgccaatct ggtaagtctt ttcagccgct ggcacgtcca gaaatcattc tgaatcattg
     1681 cgcaccgggt accgagacca agtgcctcaa accgcaactc ccgctggaag ttgacgttaa
     1741 ttgtctcccg gagccgaatg gtgtaggtgg tggtatttcc gattcctctt ctcagggcca
     1801 aaacttcgag acttgctctc cgctgtccga ggatatcggc tctaaactcg attcttggga
     1861 agagcatatt ttctccgctt tcggcaattt ctagtaatga actagtccgt aacttgaaag
     1921 tatttcgatt tcttggcttt atatatcttg tggaaaggac gagcaacacg gttcaacact
     1981 taaagtacgc gttaagtcga caatcaacct ctggattaca aaatttgtga aagattgact
     2041 ggtattctta actatgttgc tccttttacg ctatgtggat acgctgcttt aatgcctttg
     2101 tatcatgcta ttgcttcccg tatggctttc attttctcct ccttgtataa atcctggttg
     2161 ctgtctcttt atgaggagtt gtggcccgtt gtcaggcaac gtggcgtggt gtgcactgtg
     2221 tttgctgacg caacccccac tggttggggc attgccacca cctgtcagct cctttccggg
     2281 actttcgctt tccccctccc tattgccacg gcggaactca tcgccgcctg ccttgcccgc
     2341 tgctggacag gggctcggct gttgggcact gacaattccg tggtgttgtc ggggaaatca
     2401 tcgtcctttc cttggctgct cgcctgtgtt gccacctgga ttctgcgcgg gacgtccttc
     2461 tgctacgtcc cttcggccct caatccagcg gaccttcctt cccgcggcct gctgccggct
     2521 ctgcggcctc ttccgcgtct tcgccttcgc cctcagacga gtcggatctc cctttgggcc
     2581 gcctccccgc gtcgacttta agaccaatga cttacaaggc agctgtagat cttagccact
     2641 ttttaaaaga aaagggggga ctggaagggc taattcactc ccaacgaaga caagatctgc
     2701 tttttgcttg tactgggtct ctctggttag accagatctg agcctgggag ctctctggct
     2761 aactagggaa cccactgctt aagcctcaat aaagcttgcc ttgagtgctt caagtagtgt
     2821 gtgcccgtct gttgtgtgac tctggtaact agagatccct cagacccttt tagtcagtgt
     2881 ggaaaatctc tagcagtacg tatagtagtt catgtcatct tattattcag tatttataac
     2941 ttgcaaagaa atgaatatca gagagtgaga ggaacttgtt tattgcagct tataatggtt
     3001 acaaataaag caatagcatc acaaatttca caaataaagc atttttttca ctgcattcta
     3061 gttgtggttt gtccaaactc atcaatgtat cttatcatgt ctggctctag ctatcccgcc
     3121 cctaactccg cccatcccgc ccctaactcc gcccagttcc gcccattctc cgccccatgg
     3181 ctgactaatt ttttttattt atgcagaggc cgaggccgcc tcggcctctg agctattcca
     3241 gaagtagtga ggaggctttt ttggaggcct agggacgtac ccaattcgcc ctatagtgag
     3301 tcgtattacg cgcgctcact ggccgtcgtt ttacaacgtc gtgactggga aaaccctggc
     3361 gttacccaac ttaatcgcct tgcagcacat ccccctttcg ccagctggcg taatagcgaa
     3421 gaggcccgca ccgatcgccc ttcccaacag ttgcgcagcc tgaatggcga atgggacgcg
     3481 ccctgtagcg gcgcattaag cgcggcgggt gtggtggtta cgcgcagcgt gaccgctaca
     3541 cttgccagcg ccctagcgcc cgctcctttc gctttcttcc cttcctttct cgccacgttc
     3601 gccggctttc cccgtcaagc tctaaatcgg gggctccctt tagggttccg atttagtgct
     3661 ttacggcacc tcgaccccaa aaaacttgat tagggtgatg gttcacgtag tgggccatcg
     3721 ccctgataga cggtttttcg ccctttgacg ttggagtcca cgttctttaa tagtggactc
     3781 ttgttccaaa ctggaacaac actcaaccct atctcggtct attcttttga tttataaggg
     3841 attttgccga tttcggccta ttggttaaaa aatgagctga tttaacaaaa atttaacgcg
     3901 aattttaaca aaatattaac gcttacaatt taggtggcac ttttcgggga aatgtgcgcg
     3961 gaacccctat ttgtttattt ttctaaatac attcaaatat gtatccgctc atgagacaat
     4021 aaccctgata aatgcttcaa taatattgaa aaaggaagag tatgagtatt caacatttcc
     4081 gtgtcgccct tattcccttt tttgcggcat tttgccttcc tgtttttgct cacccagaaa
     4141 cgctggtgaa agtaaaagat gctgaagatc agttgggtgc acgagtgggt tacatcgaac
     4201 tggatctcaa cagcggtaag atccttgaga gttttcgccc cgaagaacgt tttccaatga
     4261 tgagcacttt taaagttctg ctatgtggcg cggtattatc ccgtattgac gccgggcaag
     4321 agcaactcgg tcgccgcata cactattctc agaatgactt ggttgagtac tcaccagtca
     4381 cagaaaagca tcttacggat ggcatgacag taagagaatt atgcagtgct gccataacca
     4441 tgagtgataa cactgcggcc aacttacttc tgacaacgat cggaggaccg aaggagctaa
     4501 ccgctttttt gcacaacatg ggggatcatg taactcgcct tgatcgttgg gaaccggagc
     4561 tgaatgaagc cataccaaac gacgagcgtg acaccacgat gcctgtagca atggcaacaa
     4621 cgttgcgcaa actattaact ggcgaactac ttactctagc ttcccggcaa caattaatag
     4681 actggatgga ggcggataaa gttgcaggac cacttctgcg ctcggccctt ccggctggct
     4741 ggtttattgc tgataaatct ggagccggtg agcgtgggtc tcgcggtatc attgcagcac
     4801 tggggccaga tggtaagccc tcccgtatcg tagttatcta cacgacgggg agtcaggcaa
     4861 ctatggatga acgaaataga cagatcgctg agataggtgc ctcactgatt aagcattggt
     4921 aactgtcaga ccaagtttac tcatatatac tttagattga tttaaaactt catttttaat
     4981 ttaaaaggat ctaggtgaag atcctttttg ataatctcat gaccaaaatc ccttaacgtg
     5041 agttttcgtt ccactgagcg tcagaccccg tagaaaagat caaaggatct tcttgagatc
     5101 ctttttttct gcgcgtaatc tgctgcttgc aaacaaaaaa accaccgcta ccagcggtgg
     5161 tttgtttgcc ggatcaagag ctaccaactc tttttccgaa ggtaactggc ttcagcagag
     5221 cgcagatacc aaatactgtt cttctagtgt agccgtagtt aggccaccac ttcaagaact
     5281 ctgtagcacc gcctacatac ctcgctctgc taatcctgtt accagtggct gctgccagtg
     5341 gcgataagtc gtgtcttacc gggttggact caagacgata gttaccggat aaggcgcagc
     5401 ggtcgggctg aacggggggt tcgtgcacac agcccagctt ggagcgaacg acctacaccg
     5461 aactgagata cctacagcgt gagctatgag aaagcgccac gcttcccgaa gggagaaagg
     5521 cggacaggta tccggtaagc ggcagggtcg gaacaggaga gcgcacgagg gagcttccag
     5581 ggggaaacgc ctggtatctt tatagtcctg tcgggtttcg ccacctctga cttgagcgtc
     5641 gatttttgtg atgctcgtca ggggggcgga gcctatggaa aaacgccagc aacgcggcct
     5701 ttttacggtt cctggccttt tgctggcctt ttgctcacat gttctttcct gcgttatccc
     5761 ctgattctgt ggataaccgt attaccgcct ttgagtgagc tgataccgct cgccgcagcc
     5821 gaacgaccga gcgcagcgag tcagtgagcg aggaagcgga agagcgccca atacgcaaac
     5881 cgcctctccc cgcgcgttgg ccgattcatt aatgcagctg gcacgacagg tttcccgact
     5941 ggaaagcggg cagtgagcgc aacgcaatta atgtgagtta gctcactcat taggcacccc
     6001 aggctttaca ctttatgctt ccggctcgta tgttgtgtgg aattgtgagc ggataacaat
     6061 ttcacacagg aaacagctat gaccatgatt acgccaagcg cgcaattaac cctcactaaa
     6121 gggaacaaaa gctggagctg caagcttaat gtagtcttat gcaatactct tgtagtcttg
     6181 caacatggta acgatgagtt agcaacatgc cttacaagga gagaaaaagc accgtgcatg
     6241 ccgattggtg gaagtaaggt ggtacgatcg tgccttatta ggaaggcaac agacgggtct
     6301 gacatggatt ggacgaacca ctgaattgcc gcattgcaga gatattgtat ttaagtgcct
     6361 agctcgatac ataaacgggt ctctctggtt agaccagatc tgagcctggg agctctctgg
     6421 ctaactaggg aacccactgc ttaagcctca ataaagcttg ccttgagtgc ttcaagtagt
     6481 gtgtgcccgt ctgttgtgtg actctggtaa ctagagatcc ctcagaccct tttagtcagt
     6541 gtggaaaatc tctagcagtg gcgcccgaac agggacttga aagcgaaagg gaaaccagag
     6601 gagctctctc gacgcaggac tcggcttgct gaagcgcgca cggcaagagg cgaggggcgg
     6661 cgactggtga gtacgccaaa aattttgact agcggaggct agaaggagag agatgggtgc
     6721 gagagcgtca gtattaagcg ggggagaatt agatcgcgat gggaaaaaat tcggttaagg
     6781 ccagggggaa agaaaaaata taaattaaaa catatagtat gggcaagcag ggagctagaa
     6841 cgattcgcag ttaatcctgg cctgttagaa acatcagaag gctgtagaca aatactggga
     6901 cagctacaac catcccttca gacaggatca gaagaactta gatcattata taatacagta
     6961 gcaaccctct attgtgtgca tcaaaggata gagataaaag acaccaagga agctttagac
     7021 aagatagagg aagagcaaaa caaaagtaag accaccgcac agcaagcggc cgctgatctt
     7081 cagacctgga ggaggagata tgagggacaa ttggagaagt gaattatata aatataaagt
     7141 agtaaaaatt gaaccattag gagtagcacc caccaaggca aagagaagag tggtgcagag
     7201 agaaaaaaga gcagtgggaa taggagcttt gttccttggg ttcttgggag cagcaggaag
     7261 cactatgggc gcagcgtcaa tgacgctgac ggtacaggcc agacaattat tgtctggtat
     7321 agtgcagcag cagaacaatt tgctgagggc tattgaggcg caacagcatc tgttgcaact
     7381 cacagtctgg ggcatcaagc agctccaggc aagaatcctg gctgtggaaa gatacctaaa
     7441 ggatcaacag ctcctgggga tttggggttg ctctggaaaa ctcatttgca ccactgctgt
     7501 gccttggaat gctagttgga gtaataaatc tctggaacag atttggaatc acacgacctg
     7561 gatggagtgg gacagagaaa ttaacaatta cacaagctta atacactcct taattgaaga
     7621 atcgcaaaac cagcaagaaa agaatgaaca agaattattg gaattagata aatgggcaag
     7681 tttgtggaat tggtttaaca taacaaattg gctgtggtat ataaaattat tcataatgat
     7741 agtaggaggc ttggtaggtt taagaatagt ttttgctgta ctttctatag tgaatagagt
     7801 taggcaggga tattcaccat tatcgtttca gacccacctc ccaaccccga ggggacccat
     7861 gcattgcatc tcaattagtc agcaaccagg tgtggaaagt ccccaggctc cccagcaggc
     7921 agaagtatgc aaagcatgcg tctcaattag tcagcaacca tagtcccgcc cctaactccg
     7981 cccatcccgc ccctaactcc gcccagttcc gcccattctc cgccccatgg ctgactaatt
     8041 ttttttattt atgcagaggc cgaggccgcc tcggcctctg agctattcca gaagtagtga
     8101 ggaggctttt ttggaggcct aggcttttgc aaaaagcttt ctagaggtac caccggagct
     8161 taccatgacc gagtacaagc ccacggtgcg cctcgccacc cgcgacgacg tccccagggc
     8221 cgtacgcacc ctcgccgccg cgttcgccga ctaccccgcc acgcgccaca ccgtcgatcc
     8281 ggaccgccac atcgagcggg tcaccgagct gcaagaactc ttcctcacgc gcgtcgggct
     8341 cgacatcggc aaggtgtggg tcgcggacga cggcgccgcc gtggcggtct ggaccacgcc
     8401 ggagagcgtc gaagcggggg cggtgttcgc cgagatcggc ccgcgcatgg ccgagttgag
     8461 cggttcccgg ctggccgcgc agcaacagat ggaaggcctc ctggcgccgc accggcccaa
     8521 ggagcccgcg tggttcctgg ccaccgtcgg cgtctcgccc gaccaccagg gcaagggtct
     8581 gggcagcgcc gtcgtgctcc ccggagtgga ggcggccgag cgcgccgggg tgcccgcctt
     8641 cctggagacc tccgcgcccc gcaacctccc cttctacgag cggctcggct tcaccgtcac
     8701 cgccgacgtc gaggtgcccg aaggaccgcg cacctggtgc atgacccgca agcccggtgc
     8761 cagttaacga attctagatc ttgagacaaa tggcagtatt catccacaat tttaaaagaa
     8821 aaggggggat tggggggtac agtgcagggg aaagaatagt agacataata gcaacagaca
     8881 tacaaactaa agaattacaa aaacaaatta caaaaattca aaattttcgg gtttattaca
     8941 gggacagcag agatccactt tggcgccggc tcgaggcctg caggtgcaaa gatggataaa
     9001 gttttaaaca gagaggaatc tttgcagcta atggaccttc taggtcttga aaggagtggg
     9061 aatt
//
