LOCUS       sequence-353217-       13186 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 13186)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 13186)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF2639
FEATURES             Location/Qualifiers
     source          1..13186
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        1..1179
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          232..1170
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     1127..1147
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     regulatory      3200..3209
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             complement(4237..4254)
                     /codon_start=1
                     /product="thrombin recognition and cleavage site"
                     /label=thrombin site
                     /translation="LVPRGS"
     misc_feature    6124..6712
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(6177..6197)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(6595..6606)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             6784..7017
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    7095..7216
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(7132..7151)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     7186..7205
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      7256..7391
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(7411..7430)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(7412..7430)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(7440..7457)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(7440..7456)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7449..7471)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      7598..8053
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(7685..7704)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     7895..7916
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        8079..8183
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(8184..8252,8253..9044)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(8402..8421)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      9215..9803
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     9704..9723
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     9957..9974
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    10091..10112
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(10127..10132,10133..10150,10151..10157)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    10165..10181
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     10170..10192
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     10189..10205
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     10189..10205
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     10224..10244
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        10226..10244
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        10272..10498
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             10499..10679
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    10726..10851
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    11344..11577
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             11762..11806
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     promoter        12037..12247
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     promoter        12059..12255
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     rep_origin      12106..12241
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             12287..12889
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGAS"
     primer_bind     complement(12287..12306)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     12783..12803
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     misc_feature    12932..13049
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
ORIGIN
        1 ggctccggtg cccgtcagtg ggcagagcgc acatcgccca cagtccccga gaagttgggg
       61 ggaggggtcg gcaattgaac cggtgcctag agaaggtggc gcggggtaaa ctgggaaagt
      121 gatgtcgtgt actggctccg cctttttccc gagggtgggg gagaaccgta tataagtgca
      181 gtagtcgccg tgaacgttct ttttcgcaac gggtttgccg ccagaacaca ggtaagtgcc
      241 gtgtgtggtt cccgcgggcc tggcctcttt acgggttatg gcccttgcgt gccttgaatt
      301 acttccacct ggctgcagta cgtgattctt gatcccgagc ttcgggttgg aagtgggtgg
      361 gagagttcga ggccttgcgc ttaaggagcc ccttcgcctc gtgcttgagt tgaggcctgg
      421 cctgggcgct ggggccgccg cgtgcgaatc tggtggcacc ttcgcgcctg tctcgctgct
      481 ttcgataagt ctctagccat ttaaaatttt tgatgacctg ctgcgacgct ttttttctgg
      541 caagatagtc ttgtaaatgc gggccaagat ctgcacactg gtatttcggt ttttggggcc
      601 gcgggcggcg acggggcccg tgcgtcccag cgcacatgtt cggcgaggcg gggcctgcga
      661 gcgcggccac cgagaatcgg acgggggtag tctcaagctg gccggcctgc tctggtgcct
      721 ggcctcgcgc cgccgtgtat cgccccgccc tgggcggcaa ggctggcccg gtcggcacca
      781 gttgcgtgag cggaaagatg gccgcttccc ggccctgctg cagggagctc aaaatggagg
      841 acgcggcgct cgggagagcg ggcgggtgag tcacccacac aaaggaaaag ggcctttccg
      901 tcctcagccg tcgcttcatg tgactccacg gagtaccggg cgccgtccag gcacctcgat
      961 tagttctcga gcttttggag tacgtcgtct ttaggttggg gggaggggtt ttatgcgatg
     1021 gagtttcccc acactgagtg ggtggagact gaagttaggc cagcttggca cttgatgtaa
     1081 ttctccttgg aatttgccct ttttgagttt ggatcttggt tcattctcaa gcctcagaca
     1141 gtggttcaaa gtttttttct tccatttcag gtgtcgtgag gctagcaccg ccaccatgta
     1201 ttcggcccac aggcccctga tgcccgcgtc cagcgcggcc tcccgtggcc tcggcatgtt
     1261 cgtgtggacg aatgtggaac ctcgctctgt ggctgtgttc ccttggcact ccttagtccc
     1321 cttcctggca cccagccagc ctgacccctc cgtgcagccg agcgaggccc agcaacctgc
     1381 cagccaccca gtggcctcca accagagcaa agaacctgct gagtcggcag ctgttgctca
     1441 tgaacggcca ccaggtggga cagggagtgc tgaccctgag cggccccctg gagccacatg
     1501 ccctgagagc ccaggacccg gacccccaca ccctttgggg gtggtggaat ctggtaaggg
     1561 tccgcctccc accacggagg aggaggcctc cggcccccca ggagagcccc ggctggacag
     1621 tgagacagag agtgaccatg atgatgcctt cctctccatc atgtctcctg agatccagtt
     1681 gcctctaccg cccggaaaac gtcggaccca gtccctcagt gccctaccca aggaacggga
     1741 ctcatcttct gagaaggatg gacgcagccc caacaagcgg gagaaggacc acatccggcg
     1801 gcccatgaat gccttcatga tcttcagcaa gcggcaccgg gccctggtcc accagcgtca
     1861 tcccaaccag gacaaccgga ccgtcagcaa gatcctgggc gagtggtggt atgccctggg
     1921 gcccaaggag aagcagaagt accacgacct ggccttccag gtgaaggagg cccacttcaa
     1981 ggcccaccca gattggaagt ggtgcaacaa ggaccgaaag aagtccagct cagaggccaa
     2041 gcccacgagc ctggggctgg caggagggca caaggagacg cgggagcgga gcatgtcgga
     2101 gacgggcact gctgctgccc ctggggtgtc ctctgagctc ctgtccgttg cagcccagac
     2161 actcctgagc tcagacacca aggctccggg gagcagctcc tgtggggcag aacggctaca
     2221 cacagttggg ggacctggct cagcccggcc ccgagctttc tcccacagcg gggtacacag
     2281 cctggacggc ggagaagtag acagtcaggc gctacaggaa ctgacgcaga tggtgtctgg
     2341 ccctgcatcg tactctggcc caaagccttc tacccagtat ggagctccag gaccctttgc
     2401 agcccctggt gagggaggtg ccttggcggc cactgggcgg cccccgctgc tgcccacccg
     2461 agcttctcgt tctcagcgtg cggccagtga ggacatgacg agtgatgagg agcgcatggt
     2521 catctgtgag gaggaagggg atgatgatgt cattgctgac gatggcttcg gcaccactga
     2581 cattgatctc aagtgcaagg agcgggtgac cgacagcgag agtggggaca gctctgggga
     2641 ggacccagag ggcaacaagg gctttggtcg gaaggtgttt tcacctgtga tccgttcctc
     2701 ctttacccac tgccgccccc cactggaccc tgagccccca gggcccccgg atcctcctgt
     2761 agcctttggc aaaggctatg gttccgcccc atcctcctct gcgtcctcgc ctgcttcctc
     2821 ctcagcctcg gcagccacct ccttctcact gggctcagga accttcaagg cccaggagtc
     2881 tggtcagggc agcacagcgg gccccctacg gcccccaccc cctggggctg ggggtccagc
     2941 gacaccttcc aaggcaaccc ggttcctccc aatggatcct gccaccttcc ggcgcaagag
     3001 acccgaaagt gtgggtggcc tggagccacc aggcccctca gtcatcgcgg cccctcccag
     3061 cggaggagga aacatcctgc agacactggt gctgccccca aacaaggagg agcaagaggg
     3121 cggcggagcc agagtgccct ccgcccccgc cccatcactg gcctatgggg ccccagcagc
     3181 tcccctgtcc cgtcctgccg ccaccatggt caccaatgtg gtgcggcctg tcagcagcac
     3241 tcctgtgccc atcgcctcta agcccttccc cacctctggc cgggctgagg cgtctccaaa
     3301 tgacacagca ggtgccagga ctgaaatggg cactgggtct cgggtgcctg ggggctcccc
     3361 gctgggtgtc agcttagtgt attcggacaa gaagtcggca gcagccacct caccagcccc
     3421 acacttggtg gctggacccc tgctgggcac tgtggggaag gcgcctgcca ctgtcactaa
     3481 cctactggtg ggcaccccgg ggtatggggc ccctgcgccc cctgctgtcc agttcattgc
     3541 ccagggggcc cctggtggtg ggaccactgc gggctcagga gcaggtgctg ggagtggccc
     3601 caatgggcca gtacccctgg gcatcctgca accaggtgcc ctgggcaagg ctgggggaat
     3661 cacccaggta cagtacatcc tgcccacgct gccccagcag cttcaggtgg cacctgcccc
     3721 agcaccagcc cctgggacca aggcagcggc tcccagcggc cctgcaccca ccaccagcat
     3781 ccgtttcacc ctcccaccgg gcacttccac caacggcaaa gtcctggctg ccactgcacc
     3841 cactcctggc atccccatcc tgcagtctgt accctccgcc ccacccccca aagcccagtc
     3901 agtttctccc gtgcaggccc cgcccccggg tggctcagcc cagctgctgc ctgggaaggt
     3961 cctagtgcct ctggccgccc ctagcatgtc agtgcggggt ggaggggccg gccagccact
     4021 gccactggtg agcccgccct tctcagtacc tgtgcagaat ggtgcccagc cccccagcaa
     4081 gatcatccag ctgaccccgg tgcctgtgag cacacccagc ggcctggtgc cgcccctgag
     4141 cccagccaca ctccctggac ccacctctca gcctcagaag gtcctgttgc cctcctccac
     4201 cagaatcacc tatgtgcagt cagcgggcgg gcacgcgctg cccctgggta ccagccctgc
     4261 gtccagccag gctggaacag tcacctcgta cgggcccacg agctctgtag ctctaggctt
     4321 cacctcgctg gggcccagcg gccccgcctt cgtgcagccc ctgctctcag gccaagcccc
     4381 actgctggct cccggtcagg tgggcgtgtc acctgtgccc agtccccagc tgccgcctgc
     4441 ctgtgcagcc cccggaggtc ctgtcataac agcattttac tctggcagcc ctgcacccac
     4501 ctcctcagca cccctggccc agccatccca ggccccccca agcctggtct acactgtggc
     4561 caccagcaca accccacctg cagccaccat tctgcccaag ggcccgccag cccctgccac
     4621 tgccacccca gccccgacta gccctttccc cagcgccaca ggttccatga cctacagctt
     4681 agtggccccc aaggcccagc ggcccagccc gaaggccccc cagaaagtga aggcagccat
     4741 cgccagcatt cccgtggggt cctttgaggc aggtgcctct gggcggcctg gccctgcacc
     4801 ccggcagcct ctggagcctg gcccagtccg agagccaact gccccagagt ctgagcttga
     4861 ggggcagccc acaccaccag cccctccacc cctgccagag acctggactc ccacggcccg
     4921 gagcagcccc ccactgcccc cacctgctga ggagcggacc agcgccaagg gccctgagac
     4981 catggccagc aaattcccca gctcatcttc agactggcgc gtccctgggc agggcctgga
     5041 gaatcgtggg gagcctccca ctcctcccag cccggcccca gctccagctg tagcccctgg
     5101 tggcagcagc gagagcagca gtgggcgggc agccggggac accccggagc gcaaggaggc
     5161 ggctggtact ggcaagaagg tgaaggtgcg gcccccgccc ctgaagaaga cctttgactc
     5221 tgtggacaac agggtcctgt cagaagtgga cttcgaagag cgctttgctg agttgcctga
     5281 gtttcggcct gaggaggtgc tgccctcccc caccctgcag tctctggcca cctcaccccg
     5341 ggccatcctg ggctcttacc gcaagaagag gaagaactcc acggacctgg attcagcacc
     5401 cgaggacccc acctcgccca agcgcaagat gagaagacgc tccagctgca gctcggagcc
     5461 caacaccccc aagagtgcca agtgcgaggg ggacatcttc acctttgacc gtacaggtac
     5521 agaagccgag gacgtgcttg gggagctaga gtatgacaag gtgccatact cctccctgcg
     5581 gcgcaccctg gaccagcgcc gggccctggt catgcagctc tttcaggacc atggcttctt
     5641 cccgtcagcc caggccacag ccgccttcca ggcccgctat gcagacatct ttccctccaa
     5701 ggtttgtctg cagttgaaga tccgtgaggt gcgccagaag atcatgcagg ctgccactcc
     5761 cacggagcag ccccctggag ctgaggctcc tctccctgta ccgcccccca ctggcaccgc
     5821 tgctgcccct gcccccactc ccagccccgc agggggccct gaccccacct cacccagctc
     5881 ggactctggc acggcccagg ctgccccgcc actgcctcca cccccagagt cggggcctgg
     5941 acagcctggc tgggaggggg ctccccagcc ctccccccca cccccaggtc cctccacagc
     6001 tgccacaggc aggtagtaat gaactagtcc gtaacttgaa agtatttcga tttcttggct
     6061 ttatatatct tgtggaaagg acgatttctg ttcgctgtag gtcaagcaac gcgttaagtc
     6121 gacaatcaac ctctggatta caaaatttgt gaaagattga ctggtattct taactatgtt
     6181 gctcctttta cgctatgtgg atacgctgct ttaatgcctt tgtatcatgc tattgcttcc
     6241 cgtatggctt tcattttctc ctccttgtat aaatcctggt tgctgtctct ttatgaggag
     6301 ttgtggcccg ttgtcaggca acgtggcgtg gtgtgcactg tgtttgctga cgcaaccccc
     6361 actggttggg gcattgccac cacctgtcag ctcctttccg ggactttcgc tttccccctc
     6421 cctattgcca cggcggaact catcgccgcc tgccttgccc gctgctggac aggggctcgg
     6481 ctgttgggca ctgacaattc cgtggtgttg tcggggaaat catcgtcctt tccttggctg
     6541 ctcgcctgtg ttgccacctg gattctgcgc gggacgtcct tctgctacgt cccttcggcc
     6601 ctcaatccag cggaccttcc ttcccgcggc ctgctgccgg ctctgcggcc tcttccgcgt
     6661 cttcgccttc gccctcagac gagtcggatc tccctttggg ccgcctcccc gcgtcgactt
     6721 taagaccaat gacttacaag gcagctgtag atcttagcca ctttttaaaa gaaaaggggg
     6781 gactggaagg gctaattcac tcccaacgaa gacaagatct gctttttgct tgtactgggt
     6841 ctctctggtt agaccagatc tgagcctggg agctctctgg ctaactaggg aacccactgc
     6901 ttaagcctca ataaagcttg ccttgagtgc ttcaagtagt gtgtgcccgt ctgttgtgtg
     6961 actctggtaa ctagagatcc ctcagaccct tttagtcagt gtggaaaatc tctagcagta
     7021 cgtatagtag ttcatgtcat cttattattc agtatttata acttgcaaag aaatgaatat
     7081 cagagagtga gaggaacttg tttattgcag cttataatgg ttacaaataa agcaatagca
     7141 tcacaaattt cacaaataaa gcattttttt cactgcattc tagttgtggt ttgtccaaac
     7201 tcatcaatgt atcttatcat gtctggctct agctatcccg cccctaactc cgcccatccc
     7261 gcccctaact ccgcccagtt ccgcccattc tccgccccat ggctgactaa ttttttttat
     7321 ttatgcagag gccgaggccg cctcggcctc tgagctattc cagaagtagt gaggaggctt
     7381 ttttggaggc ctagggacgt acccaattcg ccctatagtg agtcgtatta cgcgcgctca
     7441 ctggccgtcg ttttacaacg tcgtgactgg gaaaaccctg gcgttaccca acttaatcgc
     7501 cttgcagcac atcccccttt cgccagctgg cgtaatagcg aagaggcccg caccgatcgc
     7561 ccttcccaac agttgcgcag cctgaatggc gaatgggacg cgccctgtag cggcgcatta
     7621 agcgcggcgg gtgtggtggt tacgcgcagc gtgaccgcta cacttgccag cgccctagcg
     7681 cccgctcctt tcgctttctt cccttccttt ctcgccacgt tcgccggctt tccccgtcaa
     7741 gctctaaatc gggggctccc tttagggttc cgatttagtg ctttacggca cctcgacccc
     7801 aaaaaacttg attagggtga tggttcacgt agtgggccat cgccctgata gacggttttt
     7861 cgccctttga cgttggagtc cacgttcttt aatagtggac tcttgttcca aactggaaca
     7921 acactcaacc ctatctcggt ctattctttt gatttataag ggattttgcc gatttcggcc
     7981 tattggttaa aaaatgagct gatttaacaa aaatttaacg cgaattttaa caaaatatta
     8041 acgcttacaa tttaggtggc acttttcggg gaaatgtgcg cggaacccct atttgtttat
     8101 ttttctaaat acattcaaat atgtatccgc tcatgagaca ataaccctga taaatgcttc
     8161 aataatattg aaaaaggaag agtatgagta ttcaacattt ccgtgtcgcc cttattccct
     8221 tttttgcggc attttgcctt cctgtttttg ctcacccaga aacgctggtg aaagtaaaag
     8281 atgctgaaga tcagttgggt gcacgagtgg gttacatcga actggatctc aacagcggta
     8341 agatccttga gagttttcgc cccgaagaac gttttccaat gatgagcact tttaaagttc
     8401 tgctatgtgg cgcggtatta tcccgtattg acgccgggca agagcaactc ggtcgccgca
     8461 tacactattc tcagaatgac ttggttgagt actcaccagt cacagaaaag catcttacgg
     8521 atggcatgac agtaagagaa ttatgcagtg ctgccataac catgagtgat aacactgcgg
     8581 ccaacttact tctgacaacg atcggaggac cgaaggagct aaccgctttt ttgcacaaca
     8641 tgggggatca tgtaactcgc cttgatcgtt gggaaccgga gctgaatgaa gccataccaa
     8701 acgacgagcg tgacaccacg atgcctgtag caatggcaac aacgttgcgc aaactattaa
     8761 ctggcgaact acttactcta gcttcccggc aacaattaat agactggatg gaggcggata
     8821 aagttgcagg accacttctg cgctcggccc ttccggctgg ctggtttatt gctgataaat
     8881 ctggagccgg tgagcgtggg tctcgcggta tcattgcagc actggggcca gatggtaagc
     8941 cctcccgtat cgtagttatc tacacgacgg ggagtcaggc aactatggat gaacgaaata
     9001 gacagatcgc tgagataggt gcctcactga ttaagcattg gtaactgtca gaccaagttt
     9061 actcatatat actttagatt gatttaaaac ttcattttta atttaaaagg atctaggtga
     9121 agatcctttt tgataatctc atgaccaaaa tcccttaacg tgagttttcg ttccactgag
     9181 cgtcagaccc cgtagaaaag atcaaaggat cttcttgaga tccttttttt ctgcgcgtaa
     9241 tctgctgctt gcaaacaaaa aaaccaccgc taccagcggt ggtttgtttg ccggatcaag
     9301 agctaccaac tctttttccg aaggtaactg gcttcagcag agcgcagata ccaaatactg
     9361 ttcttctagt gtagccgtag ttaggccacc acttcaagaa ctctgtagca ccgcctacat
     9421 acctcgctct gctaatcctg ttaccagtgg ctgctgccag tggcgataag tcgtgtctta
     9481 ccgggttgga ctcaagacga tagttaccgg ataaggcgca gcggtcgggc tgaacggggg
     9541 gttcgtgcac acagcccagc ttggagcgaa cgacctacac cgaactgaga tacctacagc
     9601 gtgagctatg agaaagcgcc acgcttcccg aagggagaaa ggcggacagg tatccggtaa
     9661 gcggcagggt cggaacagga gagcgcacga gggagcttcc agggggaaac gcctggtatc
     9721 tttatagtcc tgtcgggttt cgccacctct gacttgagcg tcgatttttg tgatgctcgt
     9781 caggggggcg gagcctatgg aaaaacgcca gcaacgcggc ctttttacgg ttcctggcct
     9841 tttgctggcc ttttgctcac atgttctttc ctgcgttatc ccctgattct gtggataacc
     9901 gtattaccgc ctttgagtga gctgataccg ctcgccgcag ccgaacgacc gagcgcagcg
     9961 agtcagtgag cgaggaagcg gaagagcgcc caatacgcaa accgcctctc cccgcgcgtt
    10021 ggccgattca ttaatgcagc tggcacgaca ggtttcccga ctggaaagcg ggcagtgagc
    10081 gcaacgcaat taatgtgagt tagctcactc attaggcacc ccaggcttta cactttatgc
    10141 ttccggctcg tatgttgtgt ggaattgtga gcggataaca atttcacaca ggaaacagct
    10201 atgaccatga ttacgccaag cgcgcaatta accctcacta aagggaacaa aagctggagc
    10261 tgcaagctta atgtagtctt atgcaatact cttgtagtct tgcaacatgg taacgatgag
    10321 ttagcaacat gccttacaag gagagaaaaa gcaccgtgca tgccgattgg tggaagtaag
    10381 gtggtacgat cgtgccttat taggaaggca acagacgggt ctgacatgga ttggacgaac
    10441 cactgaattg ccgcattgca gagatattgt atttaagtgc ctagctcgat acataaacgg
    10501 gtctctctgg ttagaccaga tctgagcctg ggagctctct ggctaactag ggaacccact
    10561 gcttaagcct caataaagct tgccttgagt gcttcaagta gtgtgtgccc gtctgttgtg
    10621 tgactctggt aactagagat ccctcagacc cttttagtca gtgtggaaaa tctctagcag
    10681 tggcgcccga acagggactt gaaagcgaaa gggaaaccag aggagctctc tcgacgcagg
    10741 actcggcttg ctgaagcgcg cacggcaaga ggcgaggggc ggcgactggt gagtacgcca
    10801 aaaattttga ctagcggagg ctagaaggag agagatgggt gcgagagcgt cagtattaag
    10861 cgggggagaa ttagatcgcg atgggaaaaa attcggttaa ggccaggggg aaagaaaaaa
    10921 tataaattaa aacatatagt atgggcaagc agggagctag aacgattcgc agttaatcct
    10981 ggcctgttag aaacatcaga aggctgtaga caaatactgg gacagctaca accatccctt
    11041 cagacaggat cagaagaact tagatcatta tataatacag tagcaaccct ctattgtgtg
    11101 catcaaagga tagagataaa agacaccaag gaagctttag acaagataga ggaagagcaa
    11161 aacaaaagta agaccaccgc acagcaagcg gccgctgatc ttcagacctg gaggaggaga
    11221 tatgagggac aattggagaa gtgaattata taaatataaa gtagtaaaaa ttgaaccatt
    11281 aggagtagca cccaccaagg caaagagaag agtggtgcag agagaaaaaa gagcagtggg
    11341 aataggagct ttgttccttg ggttcttggg agcagcagga agcactatgg gcgcagcgtc
    11401 aatgacgctg acggtacagg ccagacaatt attgtctggt atagtgcagc agcagaacaa
    11461 tttgctgagg gctattgagg cgcaacagca tctgttgcaa ctcacagtct ggggcatcaa
    11521 gcagctccag gcaagaatcc tggctgtgga aagataccta aaggatcaac agctcctggg
    11581 gatttggggt tgctctggaa aactcatttg caccactgct gtgccttgga atgctagttg
    11641 gagtaataaa tctctggaac agatttggaa tcacacgacc tggatggagt gggacagaga
    11701 aattaacaat tacacaagct taatacactc cttaattgaa gaatcgcaaa accagcaaga
    11761 aaagaatgaa caagaattat tggaattaga taaatgggca agtttgtgga attggtttaa
    11821 cataacaaat tggctgtggt atataaaatt attcataatg atagtaggag gcttggtagg
    11881 tttaagaata gtttttgctg tactttctat agtgaataga gttaggcagg gatattcacc
    11941 attatcgttt cagacccacc tcccaacccc gaggggaccc atgcattgca tctcaattag
    12001 tcagcaacca ggtgtggaaa gtccccaggc tccccagcag gcagaagtat gcaaagcatg
    12061 cgtctcaatt agtcagcaac catagtcccg cccctaactc cgcccatccc gcccctaact
    12121 ccgcccagtt ccgcccattc tccgccccat ggctgactaa ttttttttat ttatgcagag
    12181 gccgaggccg cctcggcctc tgagctattc cagaagtagt gaggaggctt ttttggaggc
    12241 ctaggctttt gcaaaaagct ttctagaggt accaccggag cttaccatga ccgagtacaa
    12301 gcccacggtg cgcctcgcca cccgcgacga cgtccccagg gccgtacgca ccctcgccgc
    12361 cgcgttcgcc gactaccccg ccacgcgcca caccgtcgat ccggaccgcc acatcgagcg
    12421 ggtcaccgag ctgcaagaac tcttcctcac gcgcgtcggg ctcgacatcg gcaaggtgtg
    12481 ggtcgcggac gacggcgccg ccgtggcggt ctggaccacg ccggagagcg tcgaagcggg
    12541 ggcggtgttc gccgagatcg gcccgcgcat ggccgagttg agcggttccc ggctggccgc
    12601 gcagcaacag atggaaggcc tcctggcgcc gcaccggccc aaggagcccg cgtggttcct
    12661 ggccaccgtc ggcgtctcgc ccgaccacca gggcaagggt ctgggcagcg ccgtcgtgct
    12721 ccccggagtg gaggcggccg agcgcgccgg ggtgcccgcc ttcctggaga cctccgcgcc
    12781 ccgcaacctc cccttctacg agcggctcgg cttcaccgtc accgccgacg tcgaggtgcc
    12841 cgaaggaccg cgcacctggt gcatgacccg caagcccggt gccagttaac gaattctaga
    12901 tcttgagaca aatggcagta ttcatccaca attttaaaag aaaagggggg attggggggt
    12961 acagtgcagg ggaaagaata gtagacataa tagcaacaga catacaaact aaagaattac
    13021 aaaaacaaat tacaaaaatt caaaattttc gggtttatta cagggacagc agagatccac
    13081 tttggcgccg gctcgaggcc tgcaggtgca aagatggata aagttttaaa cagagaggaa
    13141 tctttgcagc taatggacct tctaggtctt gaaaggagtg ggaatt
//
