LOCUS       sequence-353222-        9049 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9049)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 9049)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF2662
FEATURES             Location/Qualifiers
     source          1..9049
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        1..1179
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          232..1170
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     1127..1147
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     regulatory      1190..1199
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     misc_feature    1987..2575
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(2040..2060)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(2458..2469)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             2647..2880
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    2958..3079
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(2995..3014)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     3049..3068
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      3119..3254
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(3274..3293)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(3275..3293)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(3303..3320)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(3303..3319)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(3312..3334)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      3461..3916
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(3548..3567)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     3758..3779
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        3942..4046
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(4047..4115,4116..4907)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(4265..4284)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      5078..5666
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     5567..5586
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     5820..5837
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    5954..5975
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(5990..5995,5996..6013,6014..6020)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6028..6044
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     6033..6055
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     6052..6068
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     6052..6068
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     6087..6107
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        6089..6107
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        6135..6361
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             6362..6542
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    6589..6714
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    7207..7440
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             7625..7669
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     promoter        7900..8110
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     promoter        7922..8118
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     rep_origin      7969..8104
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             8150..8752
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGAS"
     primer_bind     complement(8150..8169)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     8646..8666
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     misc_feature    8795..8912
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
ORIGIN
        1 ggctccggtg cccgtcagtg ggcagagcgc acatcgccca cagtccccga gaagttgggg
       61 ggaggggtcg gcaattgaac cggtgcctag agaaggtggc gcggggtaaa ctgggaaagt
      121 gatgtcgtgt actggctccg cctttttccc gagggtgggg gagaaccgta tataagtgca
      181 gtagtcgccg tgaacgttct ttttcgcaac gggtttgccg ccagaacaca ggtaagtgcc
      241 gtgtgtggtt cccgcgggcc tggcctcttt acgggttatg gcccttgcgt gccttgaatt
      301 acttccacct ggctgcagta cgtgattctt gatcccgagc ttcgggttgg aagtgggtgg
      361 gagagttcga ggccttgcgc ttaaggagcc ccttcgcctc gtgcttgagt tgaggcctgg
      421 cctgggcgct ggggccgccg cgtgcgaatc tggtggcacc ttcgcgcctg tctcgctgct
      481 ttcgataagt ctctagccat ttaaaatttt tgatgacctg ctgcgacgct ttttttctgg
      541 caagatagtc ttgtaaatgc gggccaagat ctgcacactg gtatttcggt ttttggggcc
      601 gcgggcggcg acggggcccg tgcgtcccag cgcacatgtt cggcgaggcg gggcctgcga
      661 gcgcggccac cgagaatcgg acgggggtag tctcaagctg gccggcctgc tctggtgcct
      721 ggcctcgcgc cgccgtgtat cgccccgccc tgggcggcaa ggctggcccg gtcggcacca
      781 gttgcgtgag cggaaagatg gccgcttccc ggccctgctg cagggagctc aaaatggagg
      841 acgcggcgct cgggagagcg ggcgggtgag tcacccacac aaaggaaaag ggcctttccg
      901 tcctcagccg tcgcttcatg tgactccacg gagtaccggg cgccgtccag gcacctcgat
      961 tagttctcga gcttttggag tacgtcgtct ttaggttggg gggaggggtt ttatgcgatg
     1021 gagtttcccc acactgagtg ggtggagact gaagttaggc cagcttggca cttgatgtaa
     1081 ttctccttgg aatttgccct ttttgagttt ggatcttggt tcattctcaa gcctcagaca
     1141 gtggttcaaa gtttttttct tccatttcag gtgtcgtgag gctagcaccg ccaccatggc
     1201 tcgtccggtt cagctggctc cgggttctct ggctctggtt ctgtgccgtc tggaagctca
     1261 gaaagctgct ggtgctgctg aagaaccggg tggtcgtgct gttttccgtg ctttccgtcg
     1321 tgctaacgct cgttgcttct ggaacgctcg tctggctcgt gctgcttctc gtctggcttt
     1381 ccagggttgg ctgcgtcgtg gtgttctgct ggttcgtgct ccgccggctt gcctgcaggt
     1441 tctgcgtgac gcttggcgtc gtcgtgctct gcgtccgccg cgtggtttcc gtatccgtgc
     1501 tgttggtgac gttttcccgg ttcagatgaa cccgatcacc cagtctcagt tcgttccgct
     1561 gggtgaagtt ctgtgctgcg ctatctctga catgaacacc gctcagatcg ttgttaccca
     1621 ggaatctctg ctggaacgtc tgatgaaaca ctacccgggt atcgctatcc cgtctgaaga
     1681 catcctgtac accaccctgg gtaccctgat caaagaacgt aaaatctacc acaccggtga
     1741 aggttacttc atcgttaccc cgcagaccta cttcatcacc aacaccacca cccaggaaaa
     1801 caaacgtatg ctgccgtctg acgaatctcg tctgatgccg gcttctatga cctacctgga
     1861 caccgaatct ggtatctagt aatgaactag tccgtaactt gaaagtattt cgatttcttg
     1921 gctttatata tcttgtggaa aggacgacaa acctactcta ttatcctatg tacgcgttaa
     1981 gtcgacaatc aacctctgga ttacaaaatt tgtgaaagat tgactggtat tcttaactat
     2041 gttgctcctt ttacgctatg tggatacgct gctttaatgc ctttgtatca tgctattgct
     2101 tcccgtatgg ctttcatttt ctcctccttg tataaatcct ggttgctgtc tctttatgag
     2161 gagttgtggc ccgttgtcag gcaacgtggc gtggtgtgca ctgtgtttgc tgacgcaacc
     2221 cccactggtt ggggcattgc caccacctgt cagctccttt ccgggacttt cgctttcccc
     2281 ctccctattg ccacggcgga actcatcgcc gcctgccttg cccgctgctg gacaggggct
     2341 cggctgttgg gcactgacaa ttccgtggtg ttgtcgggga aatcatcgtc ctttccttgg
     2401 ctgctcgcct gtgttgccac ctggattctg cgcgggacgt ccttctgcta cgtcccttcg
     2461 gccctcaatc cagcggacct tccttcccgc ggcctgctgc cggctctgcg gcctcttccg
     2521 cgtcttcgcc ttcgccctca gacgagtcgg atctcccttt gggccgcctc cccgcgtcga
     2581 ctttaagacc aatgacttac aaggcagctg tagatcttag ccacttttta aaagaaaagg
     2641 ggggactgga agggctaatt cactcccaac gaagacaaga tctgcttttt gcttgtactg
     2701 ggtctctctg gttagaccag atctgagcct gggagctctc tggctaacta gggaacccac
     2761 tgcttaagcc tcaataaagc ttgccttgag tgcttcaagt agtgtgtgcc cgtctgttgt
     2821 gtgactctgg taactagaga tccctcagac ccttttagtc agtgtggaaa atctctagca
     2881 gtacgtatag tagttcatgt catcttatta ttcagtattt ataacttgca aagaaatgaa
     2941 tatcagagag tgagaggaac ttgtttattg cagcttataa tggttacaaa taaagcaata
     3001 gcatcacaaa tttcacaaat aaagcatttt tttcactgca ttctagttgt ggtttgtcca
     3061 aactcatcaa tgtatcttat catgtctggc tctagctatc ccgcccctaa ctccgcccat
     3121 cccgccccta actccgccca gttccgccca ttctccgccc catggctgac taattttttt
     3181 tatttatgca gaggccgagg ccgcctcggc ctctgagcta ttccagaagt agtgaggagg
     3241 cttttttgga ggcctaggga cgtacccaat tcgccctata gtgagtcgta ttacgcgcgc
     3301 tcactggccg tcgttttaca acgtcgtgac tgggaaaacc ctggcgttac ccaacttaat
     3361 cgccttgcag cacatccccc tttcgccagc tggcgtaata gcgaagaggc ccgcaccgat
     3421 cgcccttccc aacagttgcg cagcctgaat ggcgaatggg acgcgccctg tagcggcgca
     3481 ttaagcgcgg cgggtgtggt ggttacgcgc agcgtgaccg ctacacttgc cagcgcccta
     3541 gcgcccgctc ctttcgcttt cttcccttcc tttctcgcca cgttcgccgg ctttccccgt
     3601 caagctctaa atcgggggct ccctttaggg ttccgattta gtgctttacg gcacctcgac
     3661 cccaaaaaac ttgattaggg tgatggttca cgtagtgggc catcgccctg atagacggtt
     3721 tttcgccctt tgacgttgga gtccacgttc tttaatagtg gactcttgtt ccaaactgga
     3781 acaacactca accctatctc ggtctattct tttgatttat aagggatttt gccgatttcg
     3841 gcctattggt taaaaaatga gctgatttaa caaaaattta acgcgaattt taacaaaata
     3901 ttaacgctta caatttaggt ggcacttttc ggggaaatgt gcgcggaacc cctatttgtt
     3961 tatttttcta aatacattca aatatgtatc cgctcatgag acaataaccc tgataaatgc
     4021 ttcaataata ttgaaaaagg aagagtatga gtattcaaca tttccgtgtc gcccttattc
     4081 ccttttttgc ggcattttgc cttcctgttt ttgctcaccc agaaacgctg gtgaaagtaa
     4141 aagatgctga agatcagttg ggtgcacgag tgggttacat cgaactggat ctcaacagcg
     4201 gtaagatcct tgagagtttt cgccccgaag aacgttttcc aatgatgagc acttttaaag
     4261 ttctgctatg tggcgcggta ttatcccgta ttgacgccgg gcaagagcaa ctcggtcgcc
     4321 gcatacacta ttctcagaat gacttggttg agtactcacc agtcacagaa aagcatctta
     4381 cggatggcat gacagtaaga gaattatgca gtgctgccat aaccatgagt gataacactg
     4441 cggccaactt acttctgaca acgatcggag gaccgaagga gctaaccgct tttttgcaca
     4501 acatggggga tcatgtaact cgccttgatc gttgggaacc ggagctgaat gaagccatac
     4561 caaacgacga gcgtgacacc acgatgcctg tagcaatggc aacaacgttg cgcaaactat
     4621 taactggcga actacttact ctagcttccc ggcaacaatt aatagactgg atggaggcgg
     4681 ataaagttgc aggaccactt ctgcgctcgg cccttccggc tggctggttt attgctgata
     4741 aatctggagc cggtgagcgt gggtctcgcg gtatcattgc agcactgggg ccagatggta
     4801 agccctcccg tatcgtagtt atctacacga cggggagtca ggcaactatg gatgaacgaa
     4861 atagacagat cgctgagata ggtgcctcac tgattaagca ttggtaactg tcagaccaag
     4921 tttactcata tatactttag attgatttaa aacttcattt ttaatttaaa aggatctagg
     4981 tgaagatcct ttttgataat ctcatgacca aaatccctta acgtgagttt tcgttccact
     5041 gagcgtcaga ccccgtagaa aagatcaaag gatcttcttg agatcctttt tttctgcgcg
     5101 taatctgctg cttgcaaaca aaaaaaccac cgctaccagc ggtggtttgt ttgccggatc
     5161 aagagctacc aactcttttt ccgaaggtaa ctggcttcag cagagcgcag ataccaaata
     5221 ctgttcttct agtgtagccg tagttaggcc accacttcaa gaactctgta gcaccgccta
     5281 catacctcgc tctgctaatc ctgttaccag tggctgctgc cagtggcgat aagtcgtgtc
     5341 ttaccgggtt ggactcaaga cgatagttac cggataaggc gcagcggtcg ggctgaacgg
     5401 ggggttcgtg cacacagccc agcttggagc gaacgaccta caccgaactg agatacctac
     5461 agcgtgagct atgagaaagc gccacgcttc ccgaagggag aaaggcggac aggtatccgg
     5521 taagcggcag ggtcggaaca ggagagcgca cgagggagct tccaggggga aacgcctggt
     5581 atctttatag tcctgtcggg tttcgccacc tctgacttga gcgtcgattt ttgtgatgct
     5641 cgtcaggggg gcggagccta tggaaaaacg ccagcaacgc ggccttttta cggttcctgg
     5701 ccttttgctg gccttttgct cacatgttct ttcctgcgtt atcccctgat tctgtggata
     5761 accgtattac cgcctttgag tgagctgata ccgctcgccg cagccgaacg accgagcgca
     5821 gcgagtcagt gagcgaggaa gcggaagagc gcccaatacg caaaccgcct ctccccgcgc
     5881 gttggccgat tcattaatgc agctggcacg acaggtttcc cgactggaaa gcgggcagtg
     5941 agcgcaacgc aattaatgtg agttagctca ctcattaggc accccaggct ttacacttta
     6001 tgcttccggc tcgtatgttg tgtggaattg tgagcggata acaatttcac acaggaaaca
     6061 gctatgacca tgattacgcc aagcgcgcaa ttaaccctca ctaaagggaa caaaagctgg
     6121 agctgcaagc ttaatgtagt cttatgcaat actcttgtag tcttgcaaca tggtaacgat
     6181 gagttagcaa catgccttac aaggagagaa aaagcaccgt gcatgccgat tggtggaagt
     6241 aaggtggtac gatcgtgcct tattaggaag gcaacagacg ggtctgacat ggattggacg
     6301 aaccactgaa ttgccgcatt gcagagatat tgtatttaag tgcctagctc gatacataaa
     6361 cgggtctctc tggttagacc agatctgagc ctgggagctc tctggctaac tagggaaccc
     6421 actgcttaag cctcaataaa gcttgccttg agtgcttcaa gtagtgtgtg cccgtctgtt
     6481 gtgtgactct ggtaactaga gatccctcag acccttttag tcagtgtgga aaatctctag
     6541 cagtggcgcc cgaacaggga cttgaaagcg aaagggaaac cagaggagct ctctcgacgc
     6601 aggactcggc ttgctgaagc gcgcacggca agaggcgagg ggcggcgact ggtgagtacg
     6661 ccaaaaattt tgactagcgg aggctagaag gagagagatg ggtgcgagag cgtcagtatt
     6721 aagcggggga gaattagatc gcgatgggaa aaaattcggt taaggccagg gggaaagaaa
     6781 aaatataaat taaaacatat agtatgggca agcagggagc tagaacgatt cgcagttaat
     6841 cctggcctgt tagaaacatc agaaggctgt agacaaatac tgggacagct acaaccatcc
     6901 cttcagacag gatcagaaga acttagatca ttatataata cagtagcaac cctctattgt
     6961 gtgcatcaaa ggatagagat aaaagacacc aaggaagctt tagacaagat agaggaagag
     7021 caaaacaaaa gtaagaccac cgcacagcaa gcggccgctg atcttcagac ctggaggagg
     7081 agatatgagg gacaattgga gaagtgaatt atataaatat aaagtagtaa aaattgaacc
     7141 attaggagta gcacccacca aggcaaagag aagagtggtg cagagagaaa aaagagcagt
     7201 gggaatagga gctttgttcc ttgggttctt gggagcagca ggaagcacta tgggcgcagc
     7261 gtcaatgacg ctgacggtac aggccagaca attattgtct ggtatagtgc agcagcagaa
     7321 caatttgctg agggctattg aggcgcaaca gcatctgttg caactcacag tctggggcat
     7381 caagcagctc caggcaagaa tcctggctgt ggaaagatac ctaaaggatc aacagctcct
     7441 ggggatttgg ggttgctctg gaaaactcat ttgcaccact gctgtgcctt ggaatgctag
     7501 ttggagtaat aaatctctgg aacagatttg gaatcacacg acctggatgg agtgggacag
     7561 agaaattaac aattacacaa gcttaataca ctccttaatt gaagaatcgc aaaaccagca
     7621 agaaaagaat gaacaagaat tattggaatt agataaatgg gcaagtttgt ggaattggtt
     7681 taacataaca aattggctgt ggtatataaa attattcata atgatagtag gaggcttggt
     7741 aggtttaaga atagtttttg ctgtactttc tatagtgaat agagttaggc agggatattc
     7801 accattatcg tttcagaccc acctcccaac cccgagggga cccatgcatt gcatctcaat
     7861 tagtcagcaa ccaggtgtgg aaagtcccca ggctccccag caggcagaag tatgcaaagc
     7921 atgcgtctca attagtcagc aaccatagtc ccgcccctaa ctccgcccat cccgccccta
     7981 actccgccca gttccgccca ttctccgccc catggctgac taattttttt tatttatgca
     8041 gaggccgagg ccgcctcggc ctctgagcta ttccagaagt agtgaggagg cttttttgga
     8101 ggcctaggct tttgcaaaaa gctttctaga ggtaccaccg gagcttacca tgaccgagta
     8161 caagcccacg gtgcgcctcg ccacccgcga cgacgtcccc agggccgtac gcaccctcgc
     8221 cgccgcgttc gccgactacc ccgccacgcg ccacaccgtc gatccggacc gccacatcga
     8281 gcgggtcacc gagctgcaag aactcttcct cacgcgcgtc gggctcgaca tcggcaaggt
     8341 gtgggtcgcg gacgacggcg ccgccgtggc ggtctggacc acgccggaga gcgtcgaagc
     8401 gggggcggtg ttcgccgaga tcggcccgcg catggccgag ttgagcggtt cccggctggc
     8461 cgcgcagcaa cagatggaag gcctcctggc gccgcaccgg cccaaggagc ccgcgtggtt
     8521 cctggccacc gtcggcgtct cgcccgacca ccagggcaag ggtctgggca gcgccgtcgt
     8581 gctccccgga gtggaggcgg ccgagcgcgc cggggtgccc gccttcctgg agacctccgc
     8641 gccccgcaac ctccccttct acgagcggct cggcttcacc gtcaccgccg acgtcgaggt
     8701 gcccgaagga ccgcgcacct ggtgcatgac ccgcaagccc ggtgccagtt aacgaattct
     8761 agatcttgag acaaatggca gtattcatcc acaattttaa aagaaaaggg gggattgggg
     8821 ggtacagtgc aggggaaaga atagtagaca taatagcaac agacatacaa actaaagaat
     8881 tacaaaaaca aattacaaaa attcaaaatt ttcgggttta ttacagggac agcagagatc
     8941 cactttggcg ccggctcgag gcctgcaggt gcaaagatgg ataaagtttt aaacagagag
     9001 gaatctttgc agctaatgga ccttctaggt cttgaaagga gtgggaatt
//
