LOCUS       sequence-353224-       10300 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10300)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 10300)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF2724
FEATURES             Location/Qualifiers
     source          1..10300
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        1..1179
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          232..1170
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     regulatory      1190..1199
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     misc_feature    3238..3826
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(3291..3311)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(3709..3720)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             3898..4131
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    4209..4330
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(4246..4265)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     4300..4319
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      4370..4505
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(4525..4544)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(4526..4544)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(4554..4571)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(4554..4570)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(4563..4585)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      4712..5167
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(4799..4818)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     5009..5030
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        5193..5297
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(5298..5366,5367..6158)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(5516..5535)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      6329..6917
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     6818..6837
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     7071..7088
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    7205..7226
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(7241..7246,7247..7264,7265..7271)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7279..7295
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     7284..7306
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     7303..7319
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     7303..7319
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     7338..7358
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        7340..7358
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        7386..7612
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             7613..7793
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    7840..7965
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    8458..8691
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             8876..8920
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     promoter        9151..9361
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     promoter        9173..9369
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     rep_origin      9220..9355
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             9401..10003
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGAS"
     primer_bind     complement(9401..9420)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     9897..9917
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     misc_feature    10046..10163
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
ORIGIN
        1 ggctccggtg cccgtcagtg ggcagagcgc acatcgccca cagtccccga gaagttgggg
       61 ggaggggtcg gcaattgaac cggtgcctag agaaggtggc gcggggtaaa ctgggaaagt
      121 gatgtcgtgt actggctccg cctttttccc gagggtgggg gagaaccgta tataagtgca
      181 gtagtcgccg tgaacgttct ttttcgcaac gggtttgccg ccagaacaca ggtaagtgcc
      241 gtgtgtggtt cccgcgggcc tggcctcttt acgggttatg gcccttgcgt gccttgaatt
      301 acttccacct ggctgcagta cgtgattctt gatcccgagc ttcgggttgg aagtgggtgg
      361 gagagttcga ggccttgcgc ttaaggagcc ccttcgcctc gtgcttgagt tgaggcctgg
      421 cctgggcgct ggggccgccg cgtgcgaatc tggtggcacc ttcgcgcctg tctcgctgct
      481 ttcgataagt ctctagccat ttaaaatttt tgatgacctg ctgcgacgct ttttttctgg
      541 caagatagtc ttgtaaatgc gggccaagat ctgcacactg gtatttcggt ttttggggcc
      601 gcgggcggcg acggggcccg tgcgtcccag cgcacatgtt cggcgaggcg gggcctgcga
      661 gcgcggccac cgagaatcgg acgggggtag tctcaagctg gccggcctgc tctggtgcct
      721 ggcctcgcgc cgccgtgtat cgccccgccc tgggcggcaa ggctggcccg gtcggcacca
      781 gttgcgtgag cggaaagatg gccgcttccc ggccctgctg cagggagctc aaaatggagg
      841 acgcggcgct cgggagagcg ggcgggtgag tcacccacac aaaggaaaag ggcctttccg
      901 tcctcagccg tcgcttcatg tgactccacg gagtaccggg cgccgtccag gcacctcgat
      961 tagttctcga gcttttggag tacgtcgtct ttaggttggg gggaggggtt ttatgcgatg
     1021 gagtttcccc acactgagtg ggtggagact gaagttaggc cagcttggca cttgatgtaa
     1081 ttctccttgg aatttgccct ttttgagttt ggatcttggt tcattctcaa gcctcagaca
     1141 gtggttcaaa gtttttttct tccatttcag gtgtcgtgag gctagcaccg ccaccatgga
     1201 gatccctgcc ccggagcccg agaagacagc tctttcctct caggatcctg ctctttccct
     1261 gaaagagaat ctcgaggata tatcgggttg gggtcttccc gaagccaggt ccaaggaatc
     1321 ggtgagtttc aaggatgtgg ctgtggactt cacccaggag gagtggggtc aactagactc
     1381 ccctcagagg gccttgtacc gggatgtgat gttggagaac taccagaacc ttcttgccct
     1441 aggacctcca ctgcacaagc cagatgtgat ctctcatctg gaacgaggcg aggagccatg
     1501 gagcatgcag agggaagtcc ccagagggcc ctgtccagaa tgggagctga aggcggtgcc
     1561 ctctcaacag cagggcattt gcaaagaaga accggcccag gagcccatca tggagcggcc
     1621 cctcggcggg gcgcaggcgt gggggcgcca ggcaggtgct ctgcagagga gtcaggctgc
     1681 gccctgggcg cccgcacctg ccatggtctg ggacgtccct gtagaggaat tccccctcag
     1741 gtgtcccctc ttcgcccagc aacgcgttcc cgagggggga cccttgctgg acacacgcaa
     1801 gaacgtccag gccactgagg gcagaaccaa ggcccccgcg agactgtgtg caggggaaaa
     1861 cgcctccacg ccaagtgagc cagaaaagtt cccccaggtg cgccggcagc gcggggcggg
     1921 cgccggggag ggcgagttcg tgtgcggcga gtgcgggaag gcgttccgcc agagctcctc
     1981 cctcacgctg caccggcgct ggcacagccg ggagaaggct tacaagtgcg atgaatgcgg
     2041 caaggccttc acctggagca ccaaccttct ggagcaccgg cgcatccaca ccggcgagaa
     2101 gcccttcttc tgcggcgagt gcgggaaggc cttcagctgc cactcgtccc tcaacgtgca
     2161 ccagcgcatc cacacgggcg agcggcccta caagtgcagc gcctgcgaga aggccttcag
     2221 ctgcagctcg ctgctcagca tgcacctgcg ggtgcacacc ggcgagaagc cctaccggtg
     2281 cggcgagtgc ggcaaggcct tcaaccagcg tacacacctc acacgccacc accgcatcca
     2341 cacgggcgag aagccctacc agtgcggctc ctgcggcaag gccttcacct gccactcatc
     2401 cctcaccgtg catgagaaga tccacagcgg ggacaagccg ttcaagtgca gcgactgcga
     2461 gaaggccttc aacagccgct cgcgcctcac cctccaccag aggacgcaca cgggcgagaa
     2521 gcccttcaag tgcgccgact gcgggaaggg cttcagctgc cacgcgtacc tgctcgtgca
     2581 ccggcgcatc cacagcggcg agaagccctt caagtgcaac gagtgcggca aagccttcag
     2641 ctcccacgcc tacctcatcg tgcaccggcg catccacaca ggcgagaagc ccttcgactg
     2701 cagccagtgt tggaaggcct tcagctgcca ctcgtccctc atcgtgcacc agcgcatcca
     2761 caccggtgag aagccctaca agtgcagcga gtgcggcaga gccttcagcc agaaccactg
     2821 tctcattaaa catcagaaaa tccactccgg ggagaagtcg tttaagtgtg agaaatgtgg
     2881 ggagatgttc aactggagct cgcacctcac tgagcaccag aggctgcaca gcgaggggaa
     2941 gcccttggcc atccagttca acaaacacct gctcagcaca tactacgtgc ctggcagcct
     3001 gctgggtgca ggggatgctg gactgaggga cgtggatccc atcgacgcgc tggatgtggc
     3061 aaagctcttg tgcgtggttc cccccagagc tggcaggaat ttctccctgg ggagcaaacc
     3121 tcgaaactag taatgaacta gtccgtaact tgaaagtatt tcgatttctt ggctttatat
     3181 atcttgtgga aaggacgacc ttgggtaata tcgccgcagt gaacgcgtta agtcgacaat
     3241 caacctctgg attacaaaat ttgtgaaaga ttgactggta ttcttaacta tgttgctcct
     3301 tttacgctat gtggatacgc tgctttaatg cctttgtatc atgctattgc ttcccgtatg
     3361 gctttcattt tctcctcctt gtataaatcc tggttgctgt ctctttatga ggagttgtgg
     3421 cccgttgtca ggcaacgtgg cgtggtgtgc actgtgtttg ctgacgcaac ccccactggt
     3481 tggggcattg ccaccacctg tcagctcctt tccgggactt tcgctttccc cctccctatt
     3541 gccacggcgg aactcatcgc cgcctgcctt gcccgctgct ggacaggggc tcggctgttg
     3601 ggcactgaca attccgtggt gttgtcgggg aaatcatcgt cctttccttg gctgctcgcc
     3661 tgtgttgcca cctggattct gcgcgggacg tccttctgct acgtcccttc ggccctcaat
     3721 ccagcggacc ttccttcccg cggcctgctg ccggctctgc ggcctcttcc gcgtcttcgc
     3781 cttcgccctc agacgagtcg gatctccctt tgggccgcct ccccgcgtcg actttaagac
     3841 caatgactta caaggcagct gtagatctta gccacttttt aaaagaaaag gggggactgg
     3901 aagggctaat tcactcccaa cgaagacaag atctgctttt tgcttgtact gggtctctct
     3961 ggttagacca gatctgagcc tgggagctct ctggctaact agggaaccca ctgcttaagc
     4021 ctcaataaag cttgccttga gtgcttcaag tagtgtgtgc ccgtctgttg tgtgactctg
     4081 gtaactagag atccctcaga cccttttagt cagtgtggaa aatctctagc agtacgtata
     4141 gtagttcatg tcatcttatt attcagtatt tataacttgc aaagaaatga atatcagaga
     4201 gtgagaggaa cttgtttatt gcagcttata atggttacaa ataaagcaat agcatcacaa
     4261 atttcacaaa taaagcattt ttttcactgc attctagttg tggtttgtcc aaactcatca
     4321 atgtatctta tcatgtctgg ctctagctat cccgccccta actccgccca tcccgcccct
     4381 aactccgccc agttccgccc attctccgcc ccatggctga ctaatttttt ttatttatgc
     4441 agaggccgag gccgcctcgg cctctgagct attccagaag tagtgaggag gcttttttgg
     4501 aggcctaggg acgtacccaa ttcgccctat agtgagtcgt attacgcgcg ctcactggcc
     4561 gtcgttttac aacgtcgtga ctgggaaaac cctggcgtta cccaacttaa tcgccttgca
     4621 gcacatcccc ctttcgccag ctggcgtaat agcgaagagg cccgcaccga tcgcccttcc
     4681 caacagttgc gcagcctgaa tggcgaatgg gacgcgccct gtagcggcgc attaagcgcg
     4741 gcgggtgtgg tggttacgcg cagcgtgacc gctacacttg ccagcgccct agcgcccgct
     4801 cctttcgctt tcttcccttc ctttctcgcc acgttcgccg gctttccccg tcaagctcta
     4861 aatcgggggc tccctttagg gttccgattt agtgctttac ggcacctcga ccccaaaaaa
     4921 cttgattagg gtgatggttc acgtagtggg ccatcgccct gatagacggt ttttcgccct
     4981 ttgacgttgg agtccacgtt ctttaatagt ggactcttgt tccaaactgg aacaacactc
     5041 aaccctatct cggtctattc ttttgattta taagggattt tgccgatttc ggcctattgg
     5101 ttaaaaaatg agctgattta acaaaaattt aacgcgaatt ttaacaaaat attaacgctt
     5161 acaatttagg tggcactttt cggggaaatg tgcgcggaac ccctatttgt ttatttttct
     5221 aaatacattc aaatatgtat ccgctcatga gacaataacc ctgataaatg cttcaataat
     5281 attgaaaaag gaagagtatg agtattcaac atttccgtgt cgcccttatt cccttttttg
     5341 cggcattttg ccttcctgtt tttgctcacc cagaaacgct ggtgaaagta aaagatgctg
     5401 aagatcagtt gggtgcacga gtgggttaca tcgaactgga tctcaacagc ggtaagatcc
     5461 ttgagagttt tcgccccgaa gaacgttttc caatgatgag cacttttaaa gttctgctat
     5521 gtggcgcggt attatcccgt attgacgccg ggcaagagca actcggtcgc cgcatacact
     5581 attctcagaa tgacttggtt gagtactcac cagtcacaga aaagcatctt acggatggca
     5641 tgacagtaag agaattatgc agtgctgcca taaccatgag tgataacact gcggccaact
     5701 tacttctgac aacgatcgga ggaccgaagg agctaaccgc ttttttgcac aacatggggg
     5761 atcatgtaac tcgccttgat cgttgggaac cggagctgaa tgaagccata ccaaacgacg
     5821 agcgtgacac cacgatgcct gtagcaatgg caacaacgtt gcgcaaacta ttaactggcg
     5881 aactacttac tctagcttcc cggcaacaat taatagactg gatggaggcg gataaagttg
     5941 caggaccact tctgcgctcg gcccttccgg ctggctggtt tattgctgat aaatctggag
     6001 ccggtgagcg tgggtctcgc ggtatcattg cagcactggg gccagatggt aagccctccc
     6061 gtatcgtagt tatctacacg acggggagtc aggcaactat ggatgaacga aatagacaga
     6121 tcgctgagat aggtgcctca ctgattaagc attggtaact gtcagaccaa gtttactcat
     6181 atatacttta gattgattta aaacttcatt tttaatttaa aaggatctag gtgaagatcc
     6241 tttttgataa tctcatgacc aaaatccctt aacgtgagtt ttcgttccac tgagcgtcag
     6301 accccgtaga aaagatcaaa ggatcttctt gagatccttt ttttctgcgc gtaatctgct
     6361 gcttgcaaac aaaaaaacca ccgctaccag cggtggtttg tttgccggat caagagctac
     6421 caactctttt tccgaaggta actggcttca gcagagcgca gataccaaat actgttcttc
     6481 tagtgtagcc gtagttaggc caccacttca agaactctgt agcaccgcct acatacctcg
     6541 ctctgctaat cctgttacca gtggctgctg ccagtggcga taagtcgtgt cttaccgggt
     6601 tggactcaag acgatagtta ccggataagg cgcagcggtc gggctgaacg gggggttcgt
     6661 gcacacagcc cagcttggag cgaacgacct acaccgaact gagataccta cagcgtgagc
     6721 tatgagaaag cgccacgctt cccgaaggga gaaaggcgga caggtatccg gtaagcggca
     6781 gggtcggaac aggagagcgc acgagggagc ttccaggggg aaacgcctgg tatctttata
     6841 gtcctgtcgg gtttcgccac ctctgacttg agcgtcgatt tttgtgatgc tcgtcagggg
     6901 ggcggagcct atggaaaaac gccagcaacg cggccttttt acggttcctg gccttttgct
     6961 ggccttttgc tcacatgttc tttcctgcgt tatcccctga ttctgtggat aaccgtatta
     7021 ccgcctttga gtgagctgat accgctcgcc gcagccgaac gaccgagcgc agcgagtcag
     7081 tgagcgagga agcggaagag cgcccaatac gcaaaccgcc tctccccgcg cgttggccga
     7141 ttcattaatg cagctggcac gacaggtttc ccgactggaa agcgggcagt gagcgcaacg
     7201 caattaatgt gagttagctc actcattagg caccccaggc tttacacttt atgcttccgg
     7261 ctcgtatgtt gtgtggaatt gtgagcggat aacaatttca cacaggaaac agctatgacc
     7321 atgattacgc caagcgcgca attaaccctc actaaaggga acaaaagctg gagctgcaag
     7381 cttaatgtag tcttatgcaa tactcttgta gtcttgcaac atggtaacga tgagttagca
     7441 acatgcctta caaggagaga aaaagcaccg tgcatgccga ttggtggaag taaggtggta
     7501 cgatcgtgcc ttattaggaa ggcaacagac gggtctgaca tggattggac gaaccactga
     7561 attgccgcat tgcagagata ttgtatttaa gtgcctagct cgatacataa acgggtctct
     7621 ctggttagac cagatctgag cctgggagct ctctggctaa ctagggaacc cactgcttaa
     7681 gcctcaataa agcttgcctt gagtgcttca agtagtgtgt gcccgtctgt tgtgtgactc
     7741 tggtaactag agatccctca gaccctttta gtcagtgtgg aaaatctcta gcagtggcgc
     7801 ccgaacaggg acttgaaagc gaaagggaaa ccagaggagc tctctcgacg caggactcgg
     7861 cttgctgaag cgcgcacggc aagaggcgag gggcggcgac tggtgagtac gccaaaaatt
     7921 ttgactagcg gaggctagaa ggagagagat gggtgcgaga gcgtcagtat taagcggggg
     7981 agaattagat cgcgatggga aaaaattcgg ttaaggccag ggggaaagaa aaaatataaa
     8041 ttaaaacata tagtatgggc aagcagggag ctagaacgat tcgcagttaa tcctggcctg
     8101 ttagaaacat cagaaggctg tagacaaata ctgggacagc tacaaccatc ccttcagaca
     8161 ggatcagaag aacttagatc attatataat acagtagcaa ccctctattg tgtgcatcaa
     8221 aggatagaga taaaagacac caaggaagct ttagacaaga tagaggaaga gcaaaacaaa
     8281 agtaagacca ccgcacagca agcggccgct gatcttcaga cctggaggag gagatatgag
     8341 ggacaattgg agaagtgaat tatataaata taaagtagta aaaattgaac cattaggagt
     8401 agcacccacc aaggcaaaga gaagagtggt gcagagagaa aaaagagcag tgggaatagg
     8461 agctttgttc cttgggttct tgggagcagc aggaagcact atgggcgcag cgtcaatgac
     8521 gctgacggta caggccagac aattattgtc tggtatagtg cagcagcaga acaatttgct
     8581 gagggctatt gaggcgcaac agcatctgtt gcaactcaca gtctggggca tcaagcagct
     8641 ccaggcaaga atcctggctg tggaaagata cctaaaggat caacagctcc tggggatttg
     8701 gggttgctct ggaaaactca tttgcaccac tgctgtgcct tggaatgcta gttggagtaa
     8761 taaatctctg gaacagattt ggaatcacac gacctggatg gagtgggaca gagaaattaa
     8821 caattacaca agcttaatac actccttaat tgaagaatcg caaaaccagc aagaaaagaa
     8881 tgaacaagaa ttattggaat tagataaatg ggcaagtttg tggaattggt ttaacataac
     8941 aaattggctg tggtatataa aattattcat aatgatagta ggaggcttgg taggtttaag
     9001 aatagttttt gctgtacttt ctatagtgaa tagagttagg cagggatatt caccattatc
     9061 gtttcagacc cacctcccaa ccccgagggg acccatgcat tgcatctcaa ttagtcagca
     9121 accaggtgtg gaaagtcccc aggctcccca gcaggcagaa gtatgcaaag catgcgtctc
     9181 aattagtcag caaccatagt cccgccccta actccgccca tcccgcccct aactccgccc
     9241 agttccgccc attctccgcc ccatggctga ctaatttttt ttatttatgc agaggccgag
     9301 gccgcctcgg cctctgagct attccagaag tagtgaggag gcttttttgg aggcctaggc
     9361 ttttgcaaaa agctttctag aggtaccacc ggagcttacc atgaccgagt acaagcccac
     9421 ggtgcgcctc gccacccgcg acgacgtccc cagggccgta cgcaccctcg ccgccgcgtt
     9481 cgccgactac cccgccacgc gccacaccgt cgatccggac cgccacatcg agcgggtcac
     9541 cgagctgcaa gaactcttcc tcacgcgcgt cgggctcgac atcggcaagg tgtgggtcgc
     9601 ggacgacggc gccgccgtgg cggtctggac cacgccggag agcgtcgaag cgggggcggt
     9661 gttcgccgag atcggcccgc gcatggccga gttgagcggt tcccggctgg ccgcgcagca
     9721 acagatggaa ggcctcctgg cgccgcaccg gcccaaggag cccgcgtggt tcctggccac
     9781 cgtcggcgtc tcgcccgacc accagggcaa gggtctgggc agcgccgtcg tgctccccgg
     9841 agtggaggcg gccgagcgcg ccggggtgcc cgccttcctg gagacctccg cgccccgcaa
     9901 cctccccttc tacgagcggc tcggcttcac cgtcaccgcc gacgtcgagg tgcccgaagg
     9961 accgcgcacc tggtgcatga cccgcaagcc cggtgccagt taacgaattc tagatcttga
    10021 gacaaatggc agtattcatc cacaatttta aaagaaaagg ggggattggg gggtacagtg
    10081 caggggaaag aatagtagac ataatagcaa cagacataca aactaaagaa ttacaaaaac
    10141 aaattacaaa aattcaaaat tttcgggttt attacaggga cagcagagat ccactttggc
    10201 gccggctcga ggcctgcagg tgcaaagatg gataaagttt taaacagaga ggaatctttg
    10261 cagctaatgg accttctagg tcttgaaagg agtgggaatt
//
