LOCUS       sequence-353228-        9292 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9292)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 9292)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF2915
FEATURES             Location/Qualifiers
     source          1..9292
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        1..1179
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          232..1170
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     1127..1147
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     misc_feature    2230..2818
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(2283..2303)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(2701..2712)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             2890..3123
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    3201..3322
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(3238..3257)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     3292..3311
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      3362..3497
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(3517..3536)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(3518..3536)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(3546..3563)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(3546..3562)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(3555..3577)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      3704..4159
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(3791..3810)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     4001..4022
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        4185..4289
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(4290..4358,4359..5150)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(4508..4527)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      5321..5909
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     5810..5829
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     6063..6080
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    6197..6218
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(6233..6238,6239..6256,6257..6263)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6271..6287
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     6276..6298
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     6295..6311
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     6295..6311
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     6330..6350
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        6332..6350
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        6378..6604
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             6605..6785
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    6832..6957
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    7450..7683
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             7868..7912
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     promoter        8143..8353
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     promoter        8165..8361
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     rep_origin      8212..8347
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             8393..8995
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGAS"
     primer_bind     complement(8393..8412)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     8889..8909
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     misc_feature    9038..9155
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
ORIGIN
        1 ggctccggtg cccgtcagtg ggcagagcgc acatcgccca cagtccccga gaagttgggg
       61 ggaggggtcg gcaattgaac cggtgcctag agaaggtggc gcggggtaaa ctgggaaagt
      121 gatgtcgtgt actggctccg cctttttccc gagggtgggg gagaaccgta tataagtgca
      181 gtagtcgccg tgaacgttct ttttcgcaac gggtttgccg ccagaacaca ggtaagtgcc
      241 gtgtgtggtt cccgcgggcc tggcctcttt acgggttatg gcccttgcgt gccttgaatt
      301 acttccacct ggctgcagta cgtgattctt gatcccgagc ttcgggttgg aagtgggtgg
      361 gagagttcga ggccttgcgc ttaaggagcc ccttcgcctc gtgcttgagt tgaggcctgg
      421 cctgggcgct ggggccgccg cgtgcgaatc tggtggcacc ttcgcgcctg tctcgctgct
      481 ttcgataagt ctctagccat ttaaaatttt tgatgacctg ctgcgacgct ttttttctgg
      541 caagatagtc ttgtaaatgc gggccaagat ctgcacactg gtatttcggt ttttggggcc
      601 gcgggcggcg acggggcccg tgcgtcccag cgcacatgtt cggcgaggcg gggcctgcga
      661 gcgcggccac cgagaatcgg acgggggtag tctcaagctg gccggcctgc tctggtgcct
      721 ggcctcgcgc cgccgtgtat cgccccgccc tgggcggcaa ggctggcccg gtcggcacca
      781 gttgcgtgag cggaaagatg gccgcttccc ggccctgctg cagggagctc aaaatggagg
      841 acgcggcgct cgggagagcg ggcgggtgag tcacccacac aaaggaaaag ggcctttccg
      901 tcctcagccg tcgcttcatg tgactccacg gagtaccggg cgccgtccag gcacctcgat
      961 tagttctcga gcttttggag tacgtcgtct ttaggttggg gggaggggtt ttatgcgatg
     1021 gagtttcccc acactgagtg ggtggagact gaagttaggc cagcttggca cttgatgtaa
     1081 ttctccttgg aatttgccct ttttgagttt ggatcttggt tcattctcaa gcctcagaca
     1141 gtggttcaaa gtttttttct tccatttcag gtgtcgtgag gctagcaccg ccaccatgct
     1201 cacttttctg tggcaacgtg attctggaca atcaggaagt ccaagccaca atgatcctgc
     1261 caagaatcct ccaggttacc ttgaggatag ttttgtaaaa tctggagtct tcaatgtatc
     1321 agaacttgta agagtatcca gaacgcccat aacccaggga actggagtca acttcccaat
     1381 tggagaaatc ccaagccaac catactatca tgacatgaac tcgggggtca atcttcagag
     1441 gtctctgtct tctccaccaa gcagcaaaag acccaaaact atatccatag atgaaaatat
     1501 ggaaccaagt cctacaggag acttttaccc ctctccaagt tcaccagctg ctggaagtcg
     1561 aacatggcac gaaagagatc aagatatgtc ttctccgact actatgaaga agcctgaaaa
     1621 gccattgttc agctctgcat ctccacagga ttcttcccca agactgagca ctttccccca
     1681 gcaccaccat cccggaatac ctggagttgc acacagtgtc atctcaactc gaactccacc
     1741 tccaccttca ccgttgccat ttccaacaca agctatcctt cctccagccc catcgagcta
     1801 cttttctcat ccaacaatca gatatcctcc ccacctgaat cctcaggata ctctgaagaa
     1861 ctatgtacct tcttatgacc catccagtcc acaaaccagc cagctgagga tttgtgactg
     1921 gaccatgaat caaaacggca ggcatttata ccccagtacc agtgaggata cattgggaat
     1981 tacttggcaa agtcctggta cctgggctag cttggttcct ttccaagtgt caaataggac
     2041 acccatctta ccggccaatg tccaaaatta cggtttgaac ataattggag aacctttcct
     2101 tcaagcagaa acaagcaact agtaatgaac tagtccgtaa cttgaaagta tttcgatttc
     2161 ttggctttat atatcttgtg gaaaggacga cacacaatgc gctaaagcgg ccaaacgcgt
     2221 taagtcgaca atcaacctct ggattacaaa atttgtgaaa gattgactgg tattcttaac
     2281 tatgttgctc cttttacgct atgtggatac gctgctttaa tgcctttgta tcatgctatt
     2341 gcttcccgta tggctttcat tttctcctcc ttgtataaat cctggttgct gtctctttat
     2401 gaggagttgt ggcccgttgt caggcaacgt ggcgtggtgt gcactgtgtt tgctgacgca
     2461 acccccactg gttggggcat tgccaccacc tgtcagctcc tttccgggac tttcgctttc
     2521 cccctcccta ttgccacggc ggaactcatc gccgcctgcc ttgcccgctg ctggacaggg
     2581 gctcggctgt tgggcactga caattccgtg gtgttgtcgg ggaaatcatc gtcctttcct
     2641 tggctgctcg cctgtgttgc cacctggatt ctgcgcggga cgtccttctg ctacgtccct
     2701 tcggccctca atccagcgga ccttccttcc cgcggcctgc tgccggctct gcggcctctt
     2761 ccgcgtcttc gccttcgccc tcagacgagt cggatctccc tttgggccgc ctccccgcgt
     2821 cgactttaag accaatgact tacaaggcag ctgtagatct tagccacttt ttaaaagaaa
     2881 aggggggact ggaagggcta attcactccc aacgaagaca agatctgctt tttgcttgta
     2941 ctgggtctct ctggttagac cagatctgag cctgggagct ctctggctaa ctagggaacc
     3001 cactgcttaa gcctcaataa agcttgcctt gagtgcttca agtagtgtgt gcccgtctgt
     3061 tgtgtgactc tggtaactag agatccctca gaccctttta gtcagtgtgg aaaatctcta
     3121 gcagtacgta tagtagttca tgtcatctta ttattcagta tttataactt gcaaagaaat
     3181 gaatatcaga gagtgagagg aacttgttta ttgcagctta taatggttac aaataaagca
     3241 atagcatcac aaatttcaca aataaagcat ttttttcact gcattctagt tgtggtttgt
     3301 ccaaactcat caatgtatct tatcatgtct ggctctagct atcccgcccc taactccgcc
     3361 catcccgccc ctaactccgc ccagttccgc ccattctccg ccccatggct gactaatttt
     3421 ttttatttat gcagaggccg aggccgcctc ggcctctgag ctattccaga agtagtgagg
     3481 aggctttttt ggaggcctag ggacgtaccc aattcgccct atagtgagtc gtattacgcg
     3541 cgctcactgg ccgtcgtttt acaacgtcgt gactgggaaa accctggcgt tacccaactt
     3601 aatcgccttg cagcacatcc ccctttcgcc agctggcgta atagcgaaga ggcccgcacc
     3661 gatcgccctt cccaacagtt gcgcagcctg aatggcgaat gggacgcgcc ctgtagcggc
     3721 gcattaagcg cggcgggtgt ggtggttacg cgcagcgtga ccgctacact tgccagcgcc
     3781 ctagcgcccg ctcctttcgc tttcttccct tcctttctcg ccacgttcgc cggctttccc
     3841 cgtcaagctc taaatcgggg gctcccttta gggttccgat ttagtgcttt acggcacctc
     3901 gaccccaaaa aacttgatta gggtgatggt tcacgtagtg ggccatcgcc ctgatagacg
     3961 gtttttcgcc ctttgacgtt ggagtccacg ttctttaata gtggactctt gttccaaact
     4021 ggaacaacac tcaaccctat ctcggtctat tcttttgatt tataagggat tttgccgatt
     4081 tcggcctatt ggttaaaaaa tgagctgatt taacaaaaat ttaacgcgaa ttttaacaaa
     4141 atattaacgc ttacaattta ggtggcactt ttcggggaaa tgtgcgcgga acccctattt
     4201 gtttattttt ctaaatacat tcaaatatgt atccgctcat gagacaataa ccctgataaa
     4261 tgcttcaata atattgaaaa aggaagagta tgagtattca acatttccgt gtcgccctta
     4321 ttcccttttt tgcggcattt tgccttcctg tttttgctca cccagaaacg ctggtgaaag
     4381 taaaagatgc tgaagatcag ttgggtgcac gagtgggtta catcgaactg gatctcaaca
     4441 gcggtaagat ccttgagagt tttcgccccg aagaacgttt tccaatgatg agcactttta
     4501 aagttctgct atgtggcgcg gtattatccc gtattgacgc cgggcaagag caactcggtc
     4561 gccgcataca ctattctcag aatgacttgg ttgagtactc accagtcaca gaaaagcatc
     4621 ttacggatgg catgacagta agagaattat gcagtgctgc cataaccatg agtgataaca
     4681 ctgcggccaa cttacttctg acaacgatcg gaggaccgaa ggagctaacc gcttttttgc
     4741 acaacatggg ggatcatgta actcgccttg atcgttggga accggagctg aatgaagcca
     4801 taccaaacga cgagcgtgac accacgatgc ctgtagcaat ggcaacaacg ttgcgcaaac
     4861 tattaactgg cgaactactt actctagctt cccggcaaca attaatagac tggatggagg
     4921 cggataaagt tgcaggacca cttctgcgct cggcccttcc ggctggctgg tttattgctg
     4981 ataaatctgg agccggtgag cgtgggtctc gcggtatcat tgcagcactg gggccagatg
     5041 gtaagccctc ccgtatcgta gttatctaca cgacggggag tcaggcaact atggatgaac
     5101 gaaatagaca gatcgctgag ataggtgcct cactgattaa gcattggtaa ctgtcagacc
     5161 aagtttactc atatatactt tagattgatt taaaacttca tttttaattt aaaaggatct
     5221 aggtgaagat cctttttgat aatctcatga ccaaaatccc ttaacgtgag ttttcgttcc
     5281 actgagcgtc agaccccgta gaaaagatca aaggatcttc ttgagatcct ttttttctgc
     5341 gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc agcggtggtt tgtttgccgg
     5401 atcaagagct accaactctt tttccgaagg taactggctt cagcagagcg cagataccaa
     5461 atactgttct tctagtgtag ccgtagttag gccaccactt caagaactct gtagcaccgc
     5521 ctacatacct cgctctgcta atcctgttac cagtggctgc tgccagtggc gataagtcgt
     5581 gtcttaccgg gttggactca agacgatagt taccggataa ggcgcagcgg tcgggctgaa
     5641 cggggggttc gtgcacacag cccagcttgg agcgaacgac ctacaccgaa ctgagatacc
     5701 tacagcgtga gctatgagaa agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc
     5761 cggtaagcgg cagggtcgga acaggagagc gcacgaggga gcttccaggg ggaaacgcct
     5821 ggtatcttta tagtcctgtc gggtttcgcc acctctgact tgagcgtcga tttttgtgat
     5881 gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa cgcggccttt ttacggttcc
     5941 tggccttttg ctggcctttt gctcacatgt tctttcctgc gttatcccct gattctgtgg
     6001 ataaccgtat taccgccttt gagtgagctg ataccgctcg ccgcagccga acgaccgagc
     6061 gcagcgagtc agtgagcgag gaagcggaag agcgcccaat acgcaaaccg cctctccccg
     6121 cgcgttggcc gattcattaa tgcagctggc acgacaggtt tcccgactgg aaagcgggca
     6181 gtgagcgcaa cgcaattaat gtgagttagc tcactcatta ggcaccccag gctttacact
     6241 ttatgcttcc ggctcgtatg ttgtgtggaa ttgtgagcgg ataacaattt cacacaggaa
     6301 acagctatga ccatgattac gccaagcgcg caattaaccc tcactaaagg gaacaaaagc
     6361 tggagctgca agcttaatgt agtcttatgc aatactcttg tagtcttgca acatggtaac
     6421 gatgagttag caacatgcct tacaaggaga gaaaaagcac cgtgcatgcc gattggtgga
     6481 agtaaggtgg tacgatcgtg ccttattagg aaggcaacag acgggtctga catggattgg
     6541 acgaaccact gaattgccgc attgcagaga tattgtattt aagtgcctag ctcgatacat
     6601 aaacgggtct ctctggttag accagatctg agcctgggag ctctctggct aactagggaa
     6661 cccactgctt aagcctcaat aaagcttgcc ttgagtgctt caagtagtgt gtgcccgtct
     6721 gttgtgtgac tctggtaact agagatccct cagacccttt tagtcagtgt ggaaaatctc
     6781 tagcagtggc gcccgaacag ggacttgaaa gcgaaaggga aaccagagga gctctctcga
     6841 cgcaggactc ggcttgctga agcgcgcacg gcaagaggcg aggggcggcg actggtgagt
     6901 acgccaaaaa ttttgactag cggaggctag aaggagagag atgggtgcga gagcgtcagt
     6961 attaagcggg ggagaattag atcgcgatgg gaaaaaattc ggttaaggcc agggggaaag
     7021 aaaaaatata aattaaaaca tatagtatgg gcaagcaggg agctagaacg attcgcagtt
     7081 aatcctggcc tgttagaaac atcagaaggc tgtagacaaa tactgggaca gctacaacca
     7141 tcccttcaga caggatcaga agaacttaga tcattatata atacagtagc aaccctctat
     7201 tgtgtgcatc aaaggataga gataaaagac accaaggaag ctttagacaa gatagaggaa
     7261 gagcaaaaca aaagtaagac caccgcacag caagcggccg ctgatcttca gacctggagg
     7321 aggagatatg agggacaatt ggagaagtga attatataaa tataaagtag taaaaattga
     7381 accattagga gtagcaccca ccaaggcaaa gagaagagtg gtgcagagag aaaaaagagc
     7441 agtgggaata ggagctttgt tccttgggtt cttgggagca gcaggaagca ctatgggcgc
     7501 agcgtcaatg acgctgacgg tacaggccag acaattattg tctggtatag tgcagcagca
     7561 gaacaatttg ctgagggcta ttgaggcgca acagcatctg ttgcaactca cagtctgggg
     7621 catcaagcag ctccaggcaa gaatcctggc tgtggaaaga tacctaaagg atcaacagct
     7681 cctggggatt tggggttgct ctggaaaact catttgcacc actgctgtgc cttggaatgc
     7741 tagttggagt aataaatctc tggaacagat ttggaatcac acgacctgga tggagtggga
     7801 cagagaaatt aacaattaca caagcttaat acactcctta attgaagaat cgcaaaacca
     7861 gcaagaaaag aatgaacaag aattattgga attagataaa tgggcaagtt tgtggaattg
     7921 gtttaacata acaaattggc tgtggtatat aaaattattc ataatgatag taggaggctt
     7981 ggtaggttta agaatagttt ttgctgtact ttctatagtg aatagagtta ggcagggata
     8041 ttcaccatta tcgtttcaga cccacctccc aaccccgagg ggacccatgc attgcatctc
     8101 aattagtcag caaccaggtg tggaaagtcc ccaggctccc cagcaggcag aagtatgcaa
     8161 agcatgcgtc tcaattagtc agcaaccata gtcccgcccc taactccgcc catcccgccc
     8221 ctaactccgc ccagttccgc ccattctccg ccccatggct gactaatttt ttttatttat
     8281 gcagaggccg aggccgcctc ggcctctgag ctattccaga agtagtgagg aggctttttt
     8341 ggaggcctag gcttttgcaa aaagctttct agaggtacca ccggagctta ccatgaccga
     8401 gtacaagccc acggtgcgcc tcgccacccg cgacgacgtc cccagggccg tacgcaccct
     8461 cgccgccgcg ttcgccgact accccgccac gcgccacacc gtcgatccgg accgccacat
     8521 cgagcgggtc accgagctgc aagaactctt cctcacgcgc gtcgggctcg acatcggcaa
     8581 ggtgtgggtc gcggacgacg gcgccgccgt ggcggtctgg accacgccgg agagcgtcga
     8641 agcgggggcg gtgttcgccg agatcggccc gcgcatggcc gagttgagcg gttcccggct
     8701 ggccgcgcag caacagatgg aaggcctcct ggcgccgcac cggcccaagg agcccgcgtg
     8761 gttcctggcc accgtcggcg tctcgcccga ccaccagggc aagggtctgg gcagcgccgt
     8821 cgtgctcccc ggagtggagg cggccgagcg cgccggggtg cccgccttcc tggagacctc
     8881 cgcgccccgc aacctcccct tctacgagcg gctcggcttc accgtcaccg ccgacgtcga
     8941 ggtgcccgaa ggaccgcgca cctggtgcat gacccgcaag cccggtgcca gttaacgaat
     9001 tctagatctt gagacaaatg gcagtattca tccacaattt taaaagaaaa ggggggattg
     9061 gggggtacag tgcaggggaa agaatagtag acataatagc aacagacata caaactaaag
     9121 aattacaaaa acaaattaca aaaattcaaa attttcgggt ttattacagg gacagcagag
     9181 atccactttg gcgccggctc gaggcctgca ggtgcaaaga tggataaagt tttaaacaga
     9241 gaggaatctt tgcagctaat ggaccttcta ggtcttgaaa ggagtgggaa tt
//
