LOCUS       sequence-35323-e         845 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 845)
  AUTHORS   Hussein R, Lim HN
  TITLE     Disruption of small RNA signaling caused by competition for Hfq.
  JOURNAL   Proc Natl Acad Sci U S A. 2011 Jan 18;108(3):1110-5. doi: 
            10.1073/pnas.1010082108. Epub 2010 Dec 28.
  PUBMED    21189298
REFERENCE   2  (bases 1 to 845)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1073/pnas.1010082108"; journalName: "Proc Natl Acad Sci U S A"; 
            date: "2011-01-18- 18"; volume: "108"; issue: "3"; pages: "1110-5"
COMMENT     Sequence Label: HL 2314 (=HL 716 + pHL 818)
FEATURES             Location/Qualifiers
     source          1..845
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    356..374
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    381..399
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    622..640
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    647..665
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     terminator      complement(732..759)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
ORIGIN
        1 ggcctcgccc tcgccctcga tctcgaactc gtggccgttc acggagccct ccatgtgcac
       61 cttgaagcgc atgaactcct tgatgatggc catgttatcc tcctcgccct tgctcaccat
      121 tttttttcct ccttagcatg ctctcacaca gcgaggccag atcgggctgc tgcaatagtg
      181 tccgagtgat gtcgaacaac ccttgttgtc cgagatcact catcggtgta tatgacattt
      241 tgttcttcca ggaaagcacc gcaaagcggt gcaaaaggtc attgactaac tataggagat
      301 ttagaatttt attagcatat acgcggaagc ggttcggcgt gtggtgtcga cagtatctct
      361 atcactgata gggatgtcaa tctctatcac tgatagggag aattcactgc tcacaagaaa
      421 aaaggcacgt catctgacgt gcctttttta tttgtactac cctgtacgat taaagcttct
      481 ggataaggat tatccaattc taaaaaaaaa gcccggacga ctgttcgggc ttgtcttttt
      541 atatgttgga aaatcagtgg caatgcaatg gcccaacaga aaataaaatc tgtgacaata
      601 aaggcatata acccgggagt atctctatca ctgataggga tgtcaatctc tatcactgat
      661 agggaggatc ctgagcggat acatatttga atgtatttag aaaaataaac aaaagagttt
      721 gtagaaacgc aaaaaggcca tccgtcagga tggccttctg cttaatttga tgcctggcag
      781 tttattggcg ggcgtcctgc ccgccaccct ccgggccgtt gcttcgcaac gttcaaatcc
      841 gctcc
//
