LOCUS       sequence-353230-         719 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  destination vector for GreenGate cloning method, contains 2x a set 
            of modules from A-F, ""Driver line"", tissues-specific promoter, 
            Dex-inducible mTurquoise2 expression.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 719)
  AUTHORS   Schurholz AK, Lopez-Salmeron V, Li Z, Forner J, Wenzl C, Gaillochet 
            C, Augustin S, Vilches-Barro A, Fuchs M, Gebert M, Lohmann JU, Greb 
            T, Wolf S
  TITLE     A Comprehensive Toolkit for Inducible, Cell Type-Specific Gene 
            Expression in Arabidopsis.
  JOURNAL   Plant Physiol. 2018 Jul 19. pii: pp.18.00463. doi: 
            10.1104/pp.18.00463.
  PUBMED    30026289
REFERENCE   2  (bases 1 to 719)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Plant 
            Physiol. 2018 Jul 19. pii: pp.18.00463. doi: 10.1104/pp.18.00463."
COMMENT     Sequence Label: pSW697 - pTMO5>>
FEATURES             Location/Qualifiers
     source          1..719
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        complement(49..95)
                     /label=minimal CaMV 35S promoter
                     /note="minimal 35S promoter from cauliflower mosaic virus"
     protein_bind    119..138
                     /label=lac operator (symmetric)
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG). The 
                     symmetric lac operator was optimized for tight binding of 
                     lac repressor."
     protein_bind    171..190
                     /label=lac operator (symmetric)
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG). The 
                     symmetric lac operator was optimized for tight binding of 
                     lac repressor."
     protein_bind    223..242
                     /label=lac operator (symmetric)
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG). The 
                     symmetric lac operator was optimized for tight binding of 
                     lac repressor."
ORIGIN
        1 agagggaaga gctaagaaga gagcgagtgt gaaggctttc attgttcgtc ctctccaaat
       61 gaaatgaact tccttatata gaggaagggt cttgcgaaga tcgatccact agtctttcaa
      121 ttgtgagcgc tcacaattct ttctcttccc tttcttcttt ctagtctttc aattgtgagc
      181 gctcacaatt ctttctcttc cctttcttct ttctagtctt tcaattgtga gcgctcacaa
      241 ttctttctct tccctttctt ctttctagat ttcttgagct cgacatatgc ataggttgtg
      301 accaggcatc atagccccta cagaacatct tgcctactca tagtcgattg atgcatgttg
      361 tcaatcaatt ggcaagtcat aaaatgcatt aaaaaatatt ttcatactca actacaaatc
      421 catgagtata actataatta taaagcaatg attagaatct gacaaggatt ctggaaaatt
      481 acataaagga aagttcataa atgtctaaaa cacaagagga catacttgta ttcagtaaca
      541 tttgcagctt ttctaggtct gaaaatatat ttgttgccta gtgaataagc ataatggtac
      601 aactacaagt gttttactcc tcatattaac ttcggtcatt agaggccacg atttgacaca
      661 tttttactca aaacaaaatg tttgcatatc tcttataatt tcaaattcaa cacacaaca
//
