LOCUS       sequence-353231-        2498 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  One-step assembly of custom RVD arrays to construct DdCBE, TALE, 
            etc..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2498)
  AUTHORS   Guo J, Zhang X, Chen X, Sun H, Dai Y, Wang J, Qian X, Tan L, Lou X, 
            Shen B
  TITLE     Precision modeling of mitochondrial diseases in zebrafish via 
            DdCBE-mediated mtDNA base editing.
  JOURNAL   Cell Discov. 2021 Sep 3;7(1):78. doi: 10.1038/s41421-021-00307-9.
  PUBMED    34480028
REFERENCE   2  (bases 1 to 2498)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell 
            Discov."; date: "2021-09-3"; volume: "7(1):78. doi"; pages: " 
            10.1038/s41421-021-00307-9"
COMMENT     Sequence Label: RVD-pA9
FEATURES             Location/Qualifiers
     source          1..2498
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      complement(37..625)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(117..136)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     928..944
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     928..944
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     949..968
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        949..967
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             complement(1142..2332)
                     /codon_start=1
                     /gene="tet"
                     /product="tetracycline efflux protein"
                     /label=TcR
                     /note="confers resistance to tetracycline"
                     /translation="MKSNNALIVILGTVTLDAVGIGLVMPVLPGLLRDIVHSDSIASHY
                     GVLLALYALMQFLCAPVLGALSDRFGRRPVLLASLLGATIDYAIMATTPVLWILYAGRI
                     VAGITGATGAVAGAYIADITDGEDRARHFGLMSACFGVGMVAGPVAGGLLGAISLHAPF
                     LAAAVLNGLNLLLGCFLMQESHKGERRPMPLRAFNPVSSFRWARGMTIVAALMTVFFIM
                     QLVGQVPAALWVIFGEDRFRWSATMIGLSLAVFGILHALAQAFVTGPATKRFGEKQAII
                     AGMAADALGYVLLAFATRGWMAFPIMILLASGGIGMPALQAMLSRQVDDDHQGQLQGSL
                     AALTSLTSIIGPLIVTAIYAASASTWNGLAWIVGAALYLVCLPALRRGAWSRATST"
     primer_bind     1961..1980
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
     promoter        complement(join(2380..2385,2386..2402,2403..2408))
                     /label=tet promoter
                     /note="E. coli promoter for tetracycline efflux protein 
                     gene"
ORIGIN
        1 ggccaggaac cgtaaaaagg ccgcgttgct ggcgtttttc cataggctcc gcccccctga
       61 cgagcatcac aaaaatcgac gctcaagtca gaggtggcga aacccgacag gactataaag
      121 ataccaggcg tttccccctg gaagctccct cgtgcgctct cctgttccga ccctgccgct
      181 taccggatac ctgtccgcct ttctcccttc gggaagcgtg gcgctttctc atagctcacg
      241 ctgtaggtat ctcagttcgg tgtaggtcgt tcgctccaag ctgggctgtg tgcacgaacc
      301 ccccgttcag cccgaccgct gcgccttatc cggtaactat cgtcttgagt ccaacccggt
      361 aagacacgac ttatcgccac tggcagcagc cactggtaac aggattagca gagcgaggta
      421 tgtaggcggt gctacagagt tcttgaagtg gtggcctaac tacggctaca ctagaagaac
      481 agtatttggt atctgcgctc tgctgaagcc agttaccttc ggaaaaagag ttggtagctc
      541 ttgatccggc aaacaaacca ccgctggtag cggtggtttt tttgtttgca agcagcagat
      601 tacgcgcaga aaaaaaggat ctcaagaaga tcctttgatc ttttctacgg ggtctgacgc
      661 tcagtggaac gacgcgtaac tcacgttaag ggattttggt catgggtggc tcgaccagat
      721 atggtctcaa gaggcaatcg ctacaacttg atctggggtg agtccgtggg cttgacagag
      781 aactggaagg aggcgctgaa ccgtctcaag agcctgtttg ccaccaatat tggaacgaga
      841 ccagctttgg tctagaggag gcatgatgat atatttttat cttgtgcaat gtaacatcag
      901 agattttgag acacgggcca gagctgccag gaaacagcta tgaccatgta atacgactca
      961 ctatagggga tattttattt tctcccaatc aggcttgatc cccagtaagt caaaaaatag
     1021 ctcgacatac tgttcttccc cgatcaaggg ttggtttgcg cattcacagt tctccgcaag
     1081 aattgattgg ctccaattct tggagtggtg aatccgttag cgaggtgccg ccggcttcca
     1141 ttcaggtcga ggtggcccgg ctccatgcac cgcgacgcaa cgcggggagg cagacaaggt
     1201 atagggcggc gcctacaatc catgccaacc cgttccatgt gctcgccgag gcggcataaa
     1261 tcgccgtgac gatcagcggt ccaatgatcg aagttaggct ggtaagagcc gcgagcgatc
     1321 cttgaagctg tccctgatgg tcgtcatcta cctgcctgga cagcatggcc tgcaacgcgg
     1381 gcatcccgat gccgccggaa gcgagaagaa tcataatggg gaaggccatc cagcctcgcg
     1441 tcgcgaacgc cagcaagacg tagcccagcg cgtcggccgc catgccggcg ataatggcct
     1501 gcttctcgcc gaaacgtttg gtggcgggac cagtgacgaa ggcttgagcg agggcgtgca
     1561 agattccgaa taccgcaagc gacaggccga tcatcgtcgc gctccagcga aagcggtcct
     1621 cgccgaaaat gacccagagc gctgccggca cctgtcctac gagttgcatg ataaagaaga
     1681 cagtcataag tgcggcgacg atagtcatgc cccgcgccca ccggaaggag ctgactgggt
     1741 tgaaggctct caagggcatg ggtcggcgct ctcccttatg cgactcctgc attaggaagc
     1801 agcccagtag taggttgagg ccgttgagca ccgccgccgc aaggaatggt gcatgtaagg
     1861 agatggcgcc caacagtccc ccggccacgg ggcctgccac catacccacg ccgaaacaag
     1921 cgctcatgag cccgaagtgg cgagcccgat cttccccatc ggtgatgtcg gcgatatagg
     1981 cgccagcaac cgcacctgtg gcgccggtga tgccggccac gatgcgtccg gcgtagagaa
     2041 tccacaggac gggtgtggtc gccatgatcg cgtagtcgat agtggctcca agtagcgaag
     2101 cgagcaggac tgggcggcgg ccaaagcggt cggacagtgc tccgagaacg ggtgcgcata
     2161 gaaattgcat caacgcatat agcgctagca gcacgccata gtgactggcg atgctgtcgg
     2221 aatggacgat atcccgcaag aggcccggca gtaccggcat aaccaagcct atgcctacag
     2281 catccagggt gacggtgccg aggatgacga tgagcgcatt gttagatttc atacacggtg
     2341 cctgactgcg ttagcaattt aactgtgata aactaccgca ttaaagctaa tcgatgataa
     2401 gctgtcaaac atgagaatta attcatgttc tttcctgcgt tatcccctga ttctgtggat
     2461 aacgcaggaa agaacatgtg agcaaaaggc cagcaaaa
//
