LOCUS       sequence-353232-        2498 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  One-step assembly of custom RVD arrays to construct DdCBE, TALE, 
            etc..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2498)
  AUTHORS   Guo J, Zhang X, Chen X, Sun H, Dai Y, Wang J, Qian X, Tan L, Lou X, 
            Shen B
  TITLE     Precision modeling of mitochondrial diseases in zebrafish via 
            DdCBE-mediated mtDNA base editing.
  JOURNAL   Cell Discov. 2021 Sep 3;7(1):78. doi: 10.1038/s41421-021-00307-9.
  PUBMED    34480028
REFERENCE   2  (bases 1 to 2498)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell 
            Discov."; date: "2021-09-3"; volume: "7(1):78. doi"; pages: " 
            10.1038/s41421-021-00307-9"
COMMENT     Sequence Label: RVD-pA10
FEATURES             Location/Qualifiers
     source          1..2498
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      complement(92..680)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(172..191)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     983..999
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     983..999
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     1004..1023
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        1004..1022
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             complement(1197..2387)
                     /codon_start=1
                     /gene="tet"
                     /product="tetracycline efflux protein"
                     /label=TcR
                     /note="confers resistance to tetracycline"
                     /translation="MKSNNALIVILGTVTLDAVGIGLVMPVLPGLLRDIVHSDSIASHY
                     GVLLALYALMQFLCAPVLGALSDRFGRRPVLLASLLGATIDYAIMATTPVLWILYAGRI
                     VAGITGATGAVAGAYIADITDGEDRARHFGLMSACFGVGMVAGPVAGGLLGAISLHAPF
                     LAAAVLNGLNLLLGCFLMQESHKGERRPMPLRAFNPVSSFRWARGMTIVAALMTVFFIM
                     QLVGQVPAALWVIFGEDRFRWSATMIGLSLAVFGILHALAQAFVTGPATKRFGEKQAII
                     AGMAADALGYVLLAFATRGWMAFPIMILLASGGIGMPALQAMLSRQVDDDHQGQLQGSL
                     AALTSLTSIIGPLIVTAIYAASASTWNGLAWIVGAALYLVCLPALRRGAWSRATST"
     primer_bind     2016..2035
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
     promoter        complement(join(2435..2440,2441..2457,2458..2463))
                     /label=tet promoter
                     /note="E. coli promoter for tetracycline efflux protein 
                     gene"
ORIGIN
        1 cccctgattc tgtggataac gcaggaaaga acatgtgagc aaaaggccag caaaaggcca
       61 ggaaccgtaa aaaggccgcg ttgctggcgt ttttccatag gctccgcccc cctgacgagc
      121 atcacaaaaa tcgacgctca agtcagaggt ggcgaaaccc gacaggacta taaagatacc
      181 aggcgtttcc ccctggaagc tccctcgtgc gctctcctgt tccgaccctg ccgcttaccg
      241 gatacctgtc cgcctttctc ccttcgggaa gcgtggcgct ttctcatagc tcacgctgta
      301 ggtatctcag ttcggtgtag gtcgttcgct ccaagctggg ctgtgtgcac gaaccccccg
      361 ttcagcccga ccgctgcgcc ttatccggta actatcgtct tgagtccaac ccggtaagac
      421 acgacttatc gccactggca gcagccactg gtaacaggat tagcagagcg aggtatgtag
      481 gcggtgctac agagttcttg aagtggtggc ctaactacgg ctacactaga agaacagtat
      541 ttggtatctg cgctctgctg aagccagtta ccttcggaaa aagagttggt agctcttgat
      601 ccggcaaaca aaccaccgct ggtagcggtg gtttttttgt ttgcaagcag cagattacgc
      661 gcagaaaaaa aggatctcaa gaagatcctt tgatcttttc tacggggtct gacgctcagt
      721 ggaacgacgc gtaactcacg ttaagggatt ttggtcatgg gtggctcgac cagatatggt
      781 ctcatgacgc aatcgctaca acttgatctg gggtgagtcc gtgggcttga cagagaactg
      841 gaaggaggcg ctgaaccgtc tcaagagcct gtttgccacc aatattagag cgagaccagc
      901 tttggtctag aggaggcatg atgatatatt tttatcttgt gcaatgtaac atcagagatt
      961 ttgagacacg ggccagagct gccaggaaac agctatgacc atgtaatacg actcactata
     1021 ggggatattt tattttctcc caatcaggct tgatccccag taagtcaaaa aatagctcga
     1081 catactgttc ttccccgatc aagggttggt ttgcgcattc acagttctcc gcaagaattg
     1141 attggctcca attcttggag tggtgaatcc gttagcgagg tgccgccggc ttccattcag
     1201 gtcgaggtgg cccggctcca tgcaccgcga cgcaacgcgg ggaggcagac aaggtatagg
     1261 gcggcgccta caatccatgc caacccgttc catgtgctcg ccgaggcggc ataaatcgcc
     1321 gtgacgatca gcggtccaat gatcgaagtt aggctggtaa gagccgcgag cgatccttga
     1381 agctgtccct gatggtcgtc atctacctgc ctggacagca tggcctgcaa cgcgggcatc
     1441 ccgatgccgc cggaagcgag aagaatcata atggggaagg ccatccagcc tcgcgtcgcg
     1501 aacgccagca agacgtagcc cagcgcgtcg gccgccatgc cggcgataat ggcctgcttc
     1561 tcgccgaaac gtttggtggc gggaccagtg acgaaggctt gagcgagggc gtgcaagatt
     1621 ccgaataccg caagcgacag gccgatcatc gtcgcgctcc agcgaaagcg gtcctcgccg
     1681 aaaatgaccc agagcgctgc cggcacctgt cctacgagtt gcatgataaa gaagacagtc
     1741 ataagtgcgg cgacgatagt catgccccgc gcccaccgga aggagctgac tgggttgaag
     1801 gctctcaagg gcatgggtcg gcgctctccc ttatgcgact cctgcattag gaagcagccc
     1861 agtagtaggt tgaggccgtt gagcaccgcc gccgcaagga atggtgcatg taaggagatg
     1921 gcgcccaaca gtcccccggc cacggggcct gccaccatac ccacgccgaa acaagcgctc
     1981 atgagcccga agtggcgagc ccgatcttcc ccatcggtga tgtcggcgat ataggcgcca
     2041 gcaaccgcac ctgtggcgcc ggtgatgccg gccacgatgc gtccggcgta gagaatccac
     2101 aggacgggtg tggtcgccat gatcgcgtag tcgatagtgg ctccaagtag cgaagcgagc
     2161 aggactgggc ggcggccaaa gcggtcggac agtgctccga gaacgggtgc gcatagaaat
     2221 tgcatcaacg catatagcgc tagcagcacg ccatagtgac tggcgatgct gtcggaatgg
     2281 acgatatccc gcaagaggcc cggcagtacc ggcataacca agcctatgcc tacagcatcc
     2341 agggtgacgg tgccgaggat gacgatgagc gcattgttag atttcataca cggtgcctga
     2401 ctgcgttagc aatttaactg tgataaacta ccgcattaaa gctaatcgat gataagctgt
     2461 caaacatgag aattaattca tgttctttcc tgcgttat
//
