LOCUS       sequence-353233-        2608 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  One-step assembly of custom RVD arrays to construct DdCBE, TALE, 
            etc..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2608)
  AUTHORS   Guo J, Zhang X, Chen X, Sun H, Dai Y, Wang J, Qian X, Tan L, Lou X, 
            Shen B
  TITLE     Precision modeling of mitochondrial diseases in zebrafish via 
            DdCBE-mediated mtDNA base editing.
  JOURNAL   Cell Discov. 2021 Sep 3;7(1):78. doi: 10.1038/s41421-021-00307-9.
  PUBMED    34480028
REFERENCE   2  (bases 1 to 2608)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell 
            Discov."; date: "2021-09-3"; volume: "7(1):78. doi"; pages: " 
            10.1038/s41421-021-00307-9"
COMMENT     Sequence Label: RVD-AG1
FEATURES             Location/Qualifiers
     source          1..2608
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     676..692
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     676..692
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     697..716
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        697..715
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             complement(890..2080)
                     /codon_start=1
                     /gene="tet"
                     /product="tetracycline efflux protein"
                     /label=TcR
                     /note="confers resistance to tetracycline"
                     /translation="MKSNNALIVILGTVTLDAVGIGLVMPVLPGLLRDIVHSDSIASHY
                     GVLLALYALMQFLCAPVLGALSDRFGRRPVLLASLLGATIDYAIMATTPVLWILYAGRI
                     VAGITGATGAVAGAYIADITDGEDRARHFGLMSACFGVGMVAGPVAGGLLGAISLHAPF
                     LAAAVLNGLNLLLGCFLMQESHKGERRPMPLRAFNPVSSFRWARGMTIVAALMTVFFIM
                     QLVGQVPAALWVIFGEDRFRWSATMIGLSLAVFGILHALAQAFVTGPATKRFGEKQAII
                     AGMAADALGYVLLAFATRGWMAFPIMILLASGGIGMPALQAMLSRQVDDDHQGQLQGSL
                     AALTSLTSIIGPLIVTAIYAASASTWNGLAWIVGAALYLVCLPALRRGAWSRATST"
     primer_bind     1709..1728
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
     promoter        complement(join(2128..2133,2134..2150,2151..2156))
                     /label=tet promoter
                     /note="E. coli promoter for tetracycline efflux protein 
                     gene"
     rep_origin      complement(join(2283..2608,1..263))
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(2363..2382)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 gacacgactt atcgccactg gcagcagcca ctggtaacag gattagcaga gcgaggtatg
       61 taggcggtgc tacagagttc ttgaagtggt ggcctaacta cggctacact agaagaacag
      121 tatttggtat ctgcgctctg ctgaagccag ttaccttcgg aaaaagagtt ggtagctctt
      181 gatccggcaa acaaaccacc gctggtagcg gtggtttttt tgtttgcaag cagcagatta
      241 cgcgcagaaa aaaaggatct caagaagatc ctttgatctt ttctacgggg tctgacgctc
      301 agtggaacga cgcgtaactc acgttaaggg attttggtca tgggtggctc gaccagatat
      361 ggtctcacct gctccggagt cagaccgtgg gcttgacaga gaactggcag taggcgctgt
      421 acggtttcaa gagcctgttt gccaccattg ttactagcaa tcgctacaac ttgatctggg
      481 gtgagtccgt gggcttgaca gagaactgga aggaggcgct gaaccgtctc aagagcctgt
      541 ttgccaccaa tattactagc gatggccacc acctgctccg gggcgagacc agctttggtc
      601 tagaggaggc atgatgatat atttttatct tgtgcaatgt aacatcagag attttgagac
      661 acgggccaga gctgccagga aacagctatg accatgtaat acgactcact ataggggata
      721 ttttattttc tcccaatcag gcttgatccc cagtaagtca aaaaatagct cgacatactg
      781 ttcttccccg atcaagggtt ggtttgcgca ttcacagttc tccgcaagaa ttgattggct
      841 ccaattcttg gagtggtgaa tccgttagcg aggtgccgcc ggcttccatt caggtcgagg
      901 tggcccggct ccatgcaccg cgacgcaacg cggggaggca gacaaggtat agggcggcgc
      961 ctacaatcca tgccaacccg ttccatgtgc tcgccgaggc ggcataaatc gccgtgacga
     1021 tcagcggtcc aatgatcgaa gttaggctgg taagagccgc gagcgatcct tgaagctgtc
     1081 cctgatggtc gtcatctacc tgcctggaca gcatggcctg caacgcgggc atcccgatgc
     1141 cgccggaagc gagaagaatc ataatgggga aggccatcca gcctcgcgtc gcgaacgcca
     1201 gcaagacgta gcccagcgcg tcggccgcca tgccggcgat aatggcctgc ttctcgccga
     1261 aacgtttggt ggcgggacca gtgacgaagg cttgagcgag ggcgtgcaag attccgaata
     1321 ccgcaagcga caggccgatc atcgtcgcgc tccagcgaaa gcggtcctcg ccgaaaatga
     1381 cccagagcgc tgccggcacc tgtcctacga gttgcatgat aaagaagaca gtcataagtg
     1441 cggcgacgat agtcatgccc cgcgcccacc ggaaggagct gactgggttg aaggctctca
     1501 agggcatggg tcggcgctct cccttatgcg actcctgcat taggaagcag cccagtagta
     1561 ggttgaggcc gttgagcacc gccgccgcaa ggaatggtgc atgtaaggag atggcgccca
     1621 acagtccccc ggccacgggg cctgccacca tacccacgcc gaaacaagcg ctcatgagcc
     1681 cgaagtggcg agcccgatct tccccatcgg tgatgtcggc gatataggcg ccagcaaccg
     1741 cacctgtggc gccggtgatg ccggccacga tgcgtccggc gtagagaatc cacaggacgg
     1801 gtgtggtcgc catgatcgcg tagtcgatag tggctccaag tagcgaagcg agcaggactg
     1861 ggcggcggcc aaagcggtcg gacagtgctc cgagaacggg tgcgcataga aattgcatca
     1921 acgcatatag cgctagcagc acgccatagt gactggcgat gctgtcggaa tggacgatat
     1981 cccgcaagag gcccggcagt accggcataa ccaagcctat gcctacagca tccagggtga
     2041 cggtgccgag gatgacgatg agcgcattgt tagatttcat acacggtgcc tgactgcgtt
     2101 agcaatttaa ctgtgataaa ctaccgcatt aaagctaatc gatgataagc tgtcaaacat
     2161 gagaattaat tcatgttctt tcctgcgtta tcccctgatt ctgtggataa cgcaggaaag
     2221 aacatgtgag caaaaggcca gcaaaaggcc aggaaccgta aaaaggccgc gttgctggcg
     2281 tttttccata ggctccgccc ccctgacgag catcacaaaa atcgacgctc aagtcagagg
     2341 tggcgaaacc cgacaggact ataaagatac caggcgtttc cccctggaag ctccctcgtg
     2401 cgctctcctg ttccgaccct gccgcttacc ggatacctgt ccgcctttct cccttcggga
     2461 agcgtggcgc tttctcatag ctcacgctgt aggtatctca gttcggtgta ggtcgttcgc
     2521 tccaagctgg gctgtgtgca cgaacccccc gttcagcccg accgctgcgc cttatccggt
     2581 aactatcgtc ttgagtccaa cccggtaa
//
