LOCUS       sequence-35324-e         863 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 863)
  AUTHORS   Hussein R, Lim HN
  TITLE     Disruption of small RNA signaling caused by competition for Hfq.
  JOURNAL   Proc Natl Acad Sci U S A. 2011 Jan 18;108(3):1110-5. doi: 
            10.1073/pnas.1010082108. Epub 2010 Dec 28.
  PUBMED    21189298
REFERENCE   2  (bases 1 to 863)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1073/pnas.1010082108"; journalName: "Proc Natl Acad Sci U S A"; 
            date: "2011-01-18- 18"; volume: "108"; issue: "3"; pages: "1110-5"
COMMENT     Sequence Label: HL 2312 (=HL 716 + pHL 787)
FEATURES             Location/Qualifiers
     source          1..863
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    333..351
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    358..376
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    599..617
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    624..642
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     terminator      complement(709..736)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
ORIGIN
        1 cggcctcgcc ctcgccctcg atctcgaact cgtggccgtt cacggagccc tccatgtgca
       61 ccttgaagcg catgaactcc ttgatgatgg ccatgttatc ctcctcgccc ttgctcacca
      121 ttttttttcc tccttagcat gctatcatga actttcagcg tattctgact cataaggtgg
      181 ctcctacccg tgatcccttg acggaacatt caagcaaaag cctggttccg ccgatttatc
      241 gctgcggcaa ataacgcagc gggtttacgg atttcccctt gtaacgaatt tcaaaatgca
      301 agcgtgttga actggttccg gtgtcgacag tatctctatc actgataggg atgtcaatct
      361 ctatcactga tagggagaat tcactgctca caagaaaaaa ggcacgtcat ctgacgtgcc
      421 ttttttattt gtactaccct gtacgattaa agcttctgga taaggattat ccaattctaa
      481 aaaaaaagcc cggacgactg ttcgggcttg tctttttata tgttggaaaa tcagtggcaa
      541 tgcaatggcc caacagaaaa taaaatctgt gacaataaag gcatataacc cgggagtatc
      601 tctatcactg atagggatgt caatctctat cactgatagg gaggatcctg agcggataca
      661 tatttgaatg tatttagaaa aataaacaaa agagtttgta gaaacgcaaa aaggccatcc
      721 gtcaggatgg ccttctgctt aatttgatgc ctggcagttt atggcgggcg tcctgcccgc
      781 caccctccgg gccgttgctt cgcaacgttc aaatccgctc ccggcggatt tgtcctactc
      841 aggagagcgt tcaccgacaa aca
//
