LOCUS       sequence-35325-e         873 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 873)
  AUTHORS   Hussein R, Lim HN
  TITLE     Disruption of small RNA signaling caused by competition for Hfq.
  JOURNAL   Proc Natl Acad Sci U S A. 2011 Jan 18;108(3):1110-5. doi: 
            10.1073/pnas.1010082108. Epub 2010 Dec 28.
  PUBMED    21189298
REFERENCE   2  (bases 1 to 873)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1073/pnas.1010082108"; journalName: "Proc Natl Acad Sci U S A"; 
            date: "2011-01-18- 18"; volume: "108"; issue: "3"; pages: "1110-5"
COMMENT     Sequence Label: HL 2308 (=HL 716 + pHL 814)
FEATURES             Location/Qualifiers
     source          1..873
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    149..165
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        223..327
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(546..565)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
ORIGIN
        1 cttctccttt acgcatgcta gctgggacca ggagggacag tactttaact ttcatgttat
       61 taaccctctg ttatatgcct ttatttgctt ttttatgcca ctgcatactg attaaccctc
      121 attaatcagt cggcaagtcg acagtatctt gttatccgct cacaatgtca atgttatccg
      181 ctcacattta tgacgtcagg tggcactttt cggggaaatg tgcgcggaac ccctatttgt
      241 ttatttttct aaatacattc aaatatgtat ccgctcatga gacaataacc ctgataaatg
      301 cttcaataat attgaaaaag gaagagtatg agtattcaac atttccgtgt cgcccttatt
      361 cccttttttg cggcattttg ccttcctgtt tttgctcacc cagaaacgct ggtgaaagta
      421 aaagatgctg aagatcagtt gggtgcacga gtgggttaca tcgaactgga tctcaacagc
      481 ggtaagatcc ttgagagttt tcgccccgaa gaacgttttc caatgatgag cacttttaaa
      541 gttctgctat gtggcgcggt attatcccgt attgacgccg ggcaagagca actcggtcgc
      601 cgcatacact attctcagaa tgacttggtt gagtactcac cagtcacaga aaagcatctt
      661 acggatggca tgacagtaag agaattatgc agtgctgcca taaccatgag tgataacact
      721 gcggccaact tacttctgac aacgatcgga ggaccgaagg agctaaccgc ttttttgcac
      781 aacatggggg atcatgtaac tcgccttgat cgttgggaac cggagctgaa tgaagccata
      841 ccaaacgacg agcgtgacac cacgatgcct gta
//
