LOCUS       sequence-35326-e         877 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 877)
  AUTHORS   Hussein R, Lim HN
  TITLE     Disruption of small RNA signaling caused by competition for Hfq.
  JOURNAL   Proc Natl Acad Sci U S A. 2011 Jan 18;108(3):1110-5. doi: 
            10.1073/pnas.1010082108. Epub 2010 Dec 28.
  PUBMED    21189298
REFERENCE   2  (bases 1 to 877)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1073/pnas.1010082108"; journalName: "Proc Natl Acad Sci U S A"; 
            date: "2011-01-18- 18"; volume: "108"; issue: "3"; pages: "1110-5"
COMMENT     Sequence Label: HL 2307 (=HL 716 + pHL 777)
FEATURES             Location/Qualifiers
     source          1..877
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    151..167
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        225..329
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(548..567)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
ORIGIN
        1 gtcttctcct ttacgcatgc tagctgggac caggagggac agtactttaa ctttcatgtt
       61 attaaccctc tgttatatgc ctttatttgc ttttttatgc cactgcatac tgattaaccc
      121 tcattaatca gtcggcaagt cgacagtatc ttgttatccg ctcacaatgt caatgttatc
      181 cgctcacatt tatgacgtca ggtggcactt ttcggggaaa tgtgcgcgga acccctattt
      241 gtttattttt ctaaatacat tcaaatatgt atccgctcat gagacaataa ccctgataaa
      301 tgcttcaata atattgaaaa aggaagagta tgagtattca acatttccgt gtcgccctta
      361 ttcccttttt tgcggcattt tgccttcctg tttttgctca cccagaaacg ctggtgaaag
      421 taaaagatgc tgaagatcag ttgggtgcac gagtgggtta catcgaactg gatctcaaca
      481 gcggtaagat ccttgagagt tttcgccccg aagaacgttt tccaatgatg agcactttta
      541 aagttctgct atgtggcgcg gtattatccc gtattgacgc cgggcaagag caactcggtc
      601 gccgcataca ctattctcag aatgacttgg ttgagtactc accagtcaca gaaaagcatc
      661 ttacggatgg catgacagta agagaattat gcagtgctgc cataaccatg agtgataaca
      721 ctgcggccaa cttacttctg acaacgatcg gaggaccgaa ggagctaacc gcttttttgc
      781 acaacatggg ggatcatgta actcgccttg atcgttggga accggagctg aatgaagcca
      841 taccaaacga cgagcgtgac accacgatgc ctgtagc
//
