LOCUS       sequence-35327-e         888 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 888)
  AUTHORS   Hussein R, Lim HN
  TITLE     Disruption of small RNA signaling caused by competition for Hfq.
  JOURNAL   Proc Natl Acad Sci U S A. 2011 Jan 18;108(3):1110-5. doi: 
            10.1073/pnas.1010082108. Epub 2010 Dec 28.
  PUBMED    21189298
REFERENCE   2  (bases 1 to 888)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1073/pnas.1010082108"; journalName: "Proc Natl Acad Sci U S A"; 
            date: "2011-01-18- 18"; volume: "108"; issue: "3"; pages: "1110-5"
COMMENT     Sequence Label: HL 2305 (=HL 716 + pHL 765)
FEATURES             Location/Qualifiers
     source          1..888
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    148..164
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        222..326
                     /gene="bla"
                     /label=AmpR promoter
     CDS             511..888
                     /label=ISS
                     /note="immunostimulatory sequence from the AmpR gene; 
                     contains unmethylated CpG dinucleotides in the context of 
                     5'-AACGTT-3' (Sato et al., 1996)"
     primer_bind     complement(545..564)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
ORIGIN
        1 ttctccttta cgcatgctag ctgggaccag gagggacagt actttaactt tcatgttatt
       61 aaccctctgt tatatgcctt tatttgcttt tttatgccac tgcatactga ttaaccctca
      121 ttaatcagtc ggcaagtcga cagtatcttg ttatccgctc acaatgtcaa tgttatccgc
      181 tcacatttat gacgtcaggt ggcacttttc ggggaaatgt gcgcggaacc cctatttgtt
      241 tatttttcta aatacattca aatatgtatc cgctcatgag acaataaccc tgataaatgc
      301 ttcaataata ttgaaaaagg aagagtatga gtattcaaca tttccgtgtc gcccttattc
      361 ccttttttgc ggcattttgc cttcctgttt ttgctcaccc agaaacgctg gtgaaagtaa
      421 aagatgctga agatcagttg ggtgcacgag tgggttacat cgaactggat ctcaacagcg
      481 gtaagatcct tgagagtttt cgccccgaag aacgttttcc aatgatgagc acttttaaag
      541 ttctgctatg tggcgcggta ttatcccgta ttgacgccgg gcaagagcaa ctcggtcgcc
      601 gcatacacta ttctcagaat gacttggttg agtactcacc agtcacagaa aagcatctta
      661 cggatggcat gacagtaaga gaattatgca gtgctgccat aaccatgagt gataacactg
      721 cggccaactt acttctgaca acgatcggag gaccgaagga gctaaccgct tttttgcaca
      781 acatggggga tcatgtaact cgccttgatc gttgggaacc ggagctgaat gaagccatac
      841 caaacgacga gcgtgacacc acgatgcctg tagcatggca acaacgtt
//
