LOCUS       sequence-353274-        4477 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Luciferase reporter for mouse Cxcl1 with one Tbx5 consensus binding 
            site.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4477)
  AUTHORS   Hall JM, Kainth AS, Rowton MJ, Lu E, Haddad G, Keplinger AJ, 
            Scepanovic J , Perez-Cervantes C, Chan SSK, Piccirilli JA, Moskowitz
            IP, Ruthenburg AJ
  TITLE     The cardiac lncRNA Chantico directly regulates Cxcl1 chemokine 
            transcription
  JOURNAL   bioRxiv 2021.12.23.474040
REFERENCE   2  (bases 1 to 4477)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "bioRxiv 
            2021.12.23.474040"
COMMENT     Sequence Label: pCxcl1-Firefly-1-Tbx5-site
FEATURES             Location/Qualifiers
     source          1..4477
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     polyA_signal    1690..1811
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(1701..1720)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     1755..1774
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     complement(2067..2084)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(2238..2826)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(2318..2337)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(3026..3817,3818..3886))
                     /codon_start=1
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     polyA_signal    3991..4039
                     /note="synthetic polyadenylation signal"
     misc_feature    4053..4144
                     /label=pause site
                     /note="RNA polymerase II transcriptional pause signal from 
                     the human alpha-2 globin gene"
     primer_bind     4093..4112
                     /label=RVprimer3
                     /note="pGL3 vector, forward primer"
     regulatory      4465..4474
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(4471..4477,1..1646)
                     /codon_start=1
                     /product="firefly luciferase"
                     /label=luciferase
                     /note="synthetic luc2 version of the luciferase gene"
                     /translation="MEDAKNIKKGPAPFYPLEDGTAGEQLHKAMKRYALVPGTIAFTDA
                     HIEVDITYAEYFEMSVRLAEAMKRYGLNTNHRIVVCSENSLQFFMPVLGALFIGVAVAP
                     ANDIYNERELLNSMGISQPTVVFVSKKGLQKILNVQKKLPIIQKIIIMDSKTDYQGFQS
                     MYTFVTSHLPPGFNEYDFVPESFDRDKTIALIMNSSGSTGLPKGVALPHRTACVRFSHA
                     RDPIFGNQIIPDTAILSVVPFHHGFGMFTTLGYLICGFRVVLMYRFEEELFLRSLQDYK
                     IQSALLVPTLFSFFAKSTLIDKYDLSNLHEIASGGAPLSKEVGEAVAKRFHLPGIRQGY
                     GLTETTSAILITPEGDDKPGAVGKVVPFFEAKVVDLDTGKTLGVNQRGELCVRGPMIMS
                     GYVNNPEATNALIDKDGWLHSGDIAYWDEDEHFFIVDRLKSLIKYKGYQVAPAELESIL
                     LQHPNIFDAGVAGLPDDDAGELPAAVVVLEHGKTMTEKEIVDYVASQVTTAKKLRGGVV
                     FVDEVPKGLTGKLDARKIREILIKAKKGGKIAV"
ORIGIN
        1 atgccaaaaa cattaagaag ggcccagcgc cattctaccc actcgaagac gggaccgccg
       61 gcgagcagct gcacaaagcc atgaagcgct acgccctggt gcccggcacc atcgccttta
      121 ccgacgcaca tatcgaggtg gacattacct acgccgagta cttcgagatg agcgttcggc
      181 tggcagaagc tatgaagcgc tatgggctga atacaaacca tcggatcgtg gtgtgcagcg
      241 agaatagctt gcagttcttc atgcccgtgt tgggtgccct gttcatcggt gtggctgtgg
      301 ccccagctaa cgacatctac aacgagcgcg agctgctgaa cagcatgggc atcagccagc
      361 ccaccgtcgt attcgtgagc aagaaagggc tgcaaaagat cctcaacgtg caaaagaagc
      421 taccgatcat acaaaagatc atcatcatgg atagcaagac cgactaccag ggcttccaaa
      481 gcatgtacac cttcgtgact tcccatttgc cacccggctt caacgagtac gacttcgtgc
      541 ccgagagctt cgaccgggac aaaaccatcg ccctgatcat gaacagtagt ggcagtaccg
      601 gattgcccaa gggcgtagcc ctaccgcacc gcaccgcttg tgtccgattc agtcatgccc
      661 gcgaccccat cttcggcaac cagatcatcc ccgacaccgc tatcctcagc gtggtgccat
      721 ttcaccacgg cttcggcatg ttcaccacgc tgggctactt gatctgcggc tttcgggtcg
      781 tgctcatgta ccgcttcgag gaggagctat tcttgcgcag cttgcaagac tataagattc
      841 aatctgccct gctggtgccc acactattta gcttcttcgc taagagcact ctcatcgaca
      901 agtacgacct aagcaacttg cacgagatcg ccagcggcgg ggcgccgctc agcaaggagg
      961 taggtgaggc cgtggccaaa cgcttccacc taccaggcat ccgccagggc tacggcctga
     1021 cagaaacaac cagcgccatt ctgatcaccc ccgaagggga cgacaagcct ggcgcagtag
     1081 gcaaggtggt gcccttcttc gaggctaagg tggtggactt ggacaccggt aagacactgg
     1141 gtgtgaacca gcgcggcgag ctgtgcgtcc gtggccccat gatcatgagc ggctacgtta
     1201 acaaccccga ggctacaaac gctctcatcg acaaggacgg ctggctgcac agcggcgaca
     1261 tcgcctactg ggacgaggac gagcacttct tcatcgtgga ccggctgaag agcctgatca
     1321 aatacaaggg ctaccaggta gccccagccg aactggagag catcctgctg caacacccca
     1381 acatcttcga cgccggggtc gccggcctgc ccgacgacga tgccggcgag ctgcccgccg
     1441 cagtcgtcgt gctggaacac ggtaaaacca tgaccgagaa ggagatcgtg gactatgtgg
     1501 ccagccaggt tacaaccgcc aagaagctgc gcggtggtgt tgtgttcgtg gacgaggtgc
     1561 ctaaaggact gaccggcaag ttggacgccc gcaagatccg cgagattctc attaaggcca
     1621 agaagggcgg caagatcgcc gtgtaataat tctagagtcg gggcggccgg ccgcttcgag
     1681 cagacatgat aagatacatt gatgagtttg gacaaaccac aactagaatg cagtgaaaaa
     1741 aatgctttat ttgtgaaatt tgtgatgcta ttgctttatt tgtaaccatt ataagctgca
     1801 ataaacaagt taacaacaac aattgcattc attttatgtt tcaggttcag ggggaggtgt
     1861 gggaggtttt ttaaagcaag taaaacctct acaaatgtgg taaaatcgat aaggatccgc
     1921 tgggtgtcag cccgatgccc ttgagagcct tcaacccagt cagctccttc cggtgggcgc
     1981 ggggcatgac tatcgtcgcc gcacttatga ctgtcttctt tatcatgcaa ctcgtaggac
     2041 aggtgccggc agcgctcttc cgcttcctcg ctcactgact cgctgcgctc ggtcgttcgg
     2101 ctgcggcgag cggtatcagc tcactcaaag gcggtaatac ggttatccac agaatcaggg
     2161 gataacgcag gaaagaacat gtgagcaaaa ggccagcaaa aggccaggaa ccgtaaaaag
     2221 gccgcgttgc tggcgttttt ccataggctc cgcccccctg acgagcatca caaaaatcga
     2281 cgctcaagtc agaggtggcg aaacccgaca ggactataaa gataccaggc gtttccccct
     2341 ggaagctccc tcgtgcgctc tcctgttccg accctgccgc ttaccggata cctgtccgcc
     2401 tttctccctt cgggaagcgt ggcgctttct catagctcac gctgtaggta tctcagttcg
     2461 gtgtaggtcg ttcgctccaa gctgggctgt gtgcacgaac cccccgttca gcccgaccgc
     2521 tgcgccttat ccggtaacta tcgtcttgag tccaacccgg taagacacga cttatcgcca
     2581 ctggcagcag ccactggtaa caggattagc agagcgaggt atgtaggcgg tgctacagag
     2641 ttcttgaagt ggtggcctaa ctacggctac actagaagaa cagtatttgg tatctgcgct
     2701 ctgctgaagc cagttacctt cggaaaaaga gttggtagct cttgatccgg caaacaaacc
     2761 accgctggta gcggtggttt ttttgtttgc aagcagcaga ttacgcgcag aaaaaaagga
     2821 tctcaagaag atcctttgat cttttctacg gggtctgacg ctcagtggaa cgaaaactca
     2881 cgttaaggga ttttggtcat gagattatca aaaaggatct tcacctagat ccttttaaat
     2941 taaaaatgaa gttttaaatc aatctaaagt atatatgagt aaacttggtc tgacagcggc
     3001 cgcaaatgct aaaccactgc agtggttacc agtgcttgat cagtgaggca ccgatctcag
     3061 cgatctgcct atttcgttcg tccatagtgg cctgactccc cgtcgtgtag atcactacga
     3121 ttcgtgaggg cttaccatca ggccccagcg cagcaatgat gccgcgagag ccgcgttcac
     3181 cggcccccga tttgtcagca atgaaccagc cagcagggag ggccgagcga agaagtggtc
     3241 ctgctacttt gtccgcctcc atccagtcta tgagctgctg tcgtgatgct agagtaagaa
     3301 gttcgccagt gagtagtttc cgaagagttg tggccattgc tactggcatc gtggtatcac
     3361 gctcgtcgtt cggtatggct tcgttcaact ctggttccca gcggtcaagc cgggtcacat
     3421 gatcacccat attatgaaga aatgcagtca gctccttagg gcctccgatc gttgtcagaa
     3481 gtaagttggc cgcggtgttg tcgctcatgg taatggcagc actacacaat tctcttaccg
     3541 tcatgccatc cgtaagatgc ttttccgtga ccggcgagta ctcaaccaag tcgttttgtg
     3601 agtagtgtat acggcgacca agctgctctt gcccggcgtc tatacgggac aacaccgcgc
     3661 cacatagcag tactttgaaa gtgctcatca tcgggaatcg ttcttcgggg cggaaagact
     3721 caaggatctt gccgctattg agatccagtt cgatatagcc cactcttgca cccagttgat
     3781 cttcagcatc ttttactttc accagcgttt cggggtgtgc aaaaacaggc aagcaaaatg
     3841 ccgcaaagaa gggaatgagt gcgacacgaa aatgttggat gctcatactc gtcctttttc
     3901 aatattattg aagcatttat cagggttact agtacgtctc tcaaggataa gtaagtaata
     3961 ttaaggtacg ggaggtattg gacaggccgc aataaaatat ctttattttc attacatctg
     4021 tgtgttggtt ttttgtgtga atcgatagta ctaacatacg ctctccatca aaacaaaacg
     4081 aaacaaaaca aactagcaaa ataggctgtc cccagtgcaa gtgcaggtgc cagaacattt
     4141 ctctggccta actggccggt acctgagctc gctagcctcg aggatatcct gaagtctcac
     4201 tactcctccc ccccccaacc ccccagccgt cacttgtcca gcgaagcacg tcactccctc
     4261 ccctttggga ctttggggca aaaagcaaaa atcccagagt ctaatccttg ggagtggagc
     4321 aagggggagg agcgatgtcc tttccggttg cagggaaaca ccctgtactc cgggaatttc
     4381 cctggcccgg agctctggag tttcgagcat aaaaggggct cgcgggagcc ctagagctct
     4441 tggcaatccg gtactgttgg taaagccacc atggaag
//
