LOCUS       sequence-353279-        4469 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Luciferase reporter for mouse Tbp.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4469)
  AUTHORS   Hall JM, Kainth AS, Rowton MJ, Lu E, Haddad G, Keplinger AJ, 
            Scepanovic J , Perez-Cervantes C, Chan SSK, Piccirilli JA, Moskowitz
            IP, Ruthenburg AJ
  TITLE     The cardiac lncRNA Chantico directly regulates Cxcl1 chemokine 
            transcription
  JOURNAL   bioRxiv 2021.12.23.474040
REFERENCE   2  (bases 1 to 4469)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "bioRxiv 
            2021.12.23.474040"
COMMENT     Sequence Label: pTbp-Firefly
FEATURES             Location/Qualifiers
     source          1..4469
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     polyA_signal    1564..1685
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(1575..1594)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     1629..1648
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     complement(1933..1950)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(2104..2692)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(2184..2203)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(2892..3683,3684..3752))
                     /codon_start=1
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     polyA_signal    3857..3905
                     /note="synthetic polyadenylation signal"
     misc_feature    3919..4010
                     /label=pause site
                     /note="RNA polymerase II transcriptional pause signal from 
                     the human alpha-2 globin gene"
     primer_bind     3959..3978
                     /label=RVprimer3
                     /note="pGL3 vector, forward primer"
     regulatory      4331..4340
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(4337..4469,1..1520)
                     /codon_start=1
                     /product="firefly luciferase"
                     /label=luciferase
                     /note="synthetic luc2 version of the luciferase gene"
                     /translation="MEDAKNIKKGPAPFYPLEDGTAGEQLHKAMKRYALVPGTIAFTDA
                     HIEVDITYAEYFEMSVRLAEAMKRYGLNTNHRIVVCSENSLQFFMPVLGALFIGVAVAP
                     ANDIYNERELLNSMGISQPTVVFVSKKGLQKILNVQKKLPIIQKIIIMDSKTDYQGFQS
                     MYTFVTSHLPPGFNEYDFVPESFDRDKTIALIMNSSGSTGLPKGVALPHRTACVRFSHA
                     RDPIFGNQIIPDTAILSVVPFHHGFGMFTTLGYLICGFRVVLMYRFEEELFLRSLQDYK
                     IQSALLVPTLFSFFAKSTLIDKYDLSNLHEIASGGAPLSKEVGEAVAKRFHLPGIRQGY
                     GLTETTSAILITPEGDDKPGAVGKVVPFFEAKVVDLDTGKTLGVNQRGELCVRGPMIMS
                     GYVNNPEATNALIDKDGWLHSGDIAYWDEDEHFFIVDRLKSLIKYKGYQVAPAELESIL
                     LQHPNIFDAGVAGLPDDDAGELPAAVVVLEHGKTMTEKEIVDYVASQVTTAKKLRGGVV
                     FVDEVPKGLTGKLDARKIREILIKAKKGGKIAV"
ORIGIN
        1 cacatatcga ggtggacatt acctacgccg agtacttcga gatgagcgtt cggctggcag
       61 aagctatgaa gcgctatggg ctgaatacaa accatcggat cgtggtgtgc agcgagaata
      121 gcttgcagtt cttcatgccc gtgttgggtg ccctgttcat cggtgtggct gtggccccag
      181 ctaacgacat ctacaacgag cgcgagctgc tgaacagcat gggcatcagc cagcccaccg
      241 tcgtattcgt gagcaagaaa gggctgcaaa agatcctcaa cgtgcaaaag aagctaccga
      301 tcatacaaaa gatcatcatc atggatagca agaccgacta ccagggcttc caaagcatgt
      361 acaccttcgt gacttcccat ttgccacccg gcttcaacga gtacgacttc gtgcccgaga
      421 gcttcgaccg ggacaaaacc atcgccctga tcatgaacag tagtggcagt accggattgc
      481 ccaagggcgt agccctaccg caccgcaccg cttgtgtccg attcagtcat gcccgcgacc
      541 ccatcttcgg caaccagatc atccccgaca ccgctatcct cagcgtggtg ccatttcacc
      601 acggcttcgg catgttcacc acgctgggct acttgatctg cggctttcgg gtcgtgctca
      661 tgtaccgctt cgaggaggag ctattcttgc gcagcttgca agactataag attcaatctg
      721 ccctgctggt gcccacacta tttagcttct tcgctaagag cactctcatc gacaagtacg
      781 acctaagcaa cttgcacgag atcgccagcg gcggggcgcc gctcagcaag gaggtaggtg
      841 aggccgtggc caaacgcttc cacctaccag gcatccgcca gggctacggc ctgacagaaa
      901 caaccagcgc cattctgatc acccccgaag gggacgacaa gcctggcgca gtaggcaagg
      961 tggtgccctt cttcgaggct aaggtggtgg acttggacac cggtaagaca ctgggtgtga
     1021 accagcgcgg cgagctgtgc gtccgtggcc ccatgatcat gagcggctac gttaacaacc
     1081 ccgaggctac aaacgctctc atcgacaagg acggctggct gcacagcggc gacatcgcct
     1141 actgggacga ggacgagcac ttcttcatcg tggaccggct gaagagcctg atcaaataca
     1201 agggctacca ggtagcccca gccgaactgg agagcatcct gctgcaacac cccaacatct
     1261 tcgacgccgg ggtcgccggc ctgcccgacg acgatgccgg cgagctgccc gccgcagtcg
     1321 tcgtgctgga acacggtaaa accatgaccg agaaggagat cgtggactat gtggccagcc
     1381 aggttacaac cgccaagaag ctgcgcggtg gtgttgtgtt cgtggacgag gtgcctaaag
     1441 gactgaccgg caagttggac gcccgcaaga tccgcgagat tctcattaag gccaagaagg
     1501 gcggcaagat cgccgtgtaa taattctaga gtcggggcgg ccggccgctt cgagcagaca
     1561 tgataagata cattgatgag tttggacaaa ccacaactag aatgcagtga aaaaaatgct
     1621 ttatttgtga aatttgtgat gctattgctt tatttgtaac cattataagc tgcaataaac
     1681 aagttaacaa caacaattgc attcatttta tgtttcaggt tcagggggag gtgtgggagg
     1741 ttttttaaag caagtaaaac ctctacaaat gtggtaaaat cgataaggat ccgtcgaccg
     1801 atgcccttga gagccttcaa cccagtcagc tccttccggt gggcgcgggg catgactatc
     1861 gtcgccgcac ttatgactgt cttctttatc atgcaactcg taggacaggt gccggcagcg
     1921 ctcttccgct tcctcgctca ctgactcgct gcgctcggtc gttcggctgc ggcgagcggt
     1981 atcagctcac tcaaaggcgg taatacggtt atccacagaa tcaggggata acgcaggaaa
     2041 gaacatgtga gcaaaaggcc agcaaaaggc caggaaccgt aaaaaggccg cgttgctggc
     2101 gtttttccat aggctccgcc cccctgacga gcatcacaaa aatcgacgct caagtcagag
     2161 gtggcgaaac ccgacaggac tataaagata ccaggcgttt ccccctggaa gctccctcgt
     2221 gcgctctcct gttccgaccc tgccgcttac cggatacctg tccgcctttc tcccttcggg
     2281 aagcgtggcg ctttctcata gctcacgctg taggtatctc agttcggtgt aggtcgttcg
     2341 ctccaagctg ggctgtgtgc acgaaccccc cgttcagccc gaccgctgcg ccttatccgg
     2401 taactatcgt cttgagtcca acccggtaag acacgactta tcgccactgg cagcagccac
     2461 tggtaacagg attagcagag cgaggtatgt aggcggtgct acagagttct tgaagtggtg
     2521 gcctaactac ggctacacta gaagaacagt atttggtatc tgcgctctgc tgaagccagt
     2581 taccttcgga aaaagagttg gtagctcttg atccggcaaa caaaccaccg ctggtagcgg
     2641 tggttttttt gtttgcaagc agcagattac gcgcagaaaa aaaggatctc aagaagatcc
     2701 tttgatcttt tctacggggt ctgacgctca gtggaacgaa aactcacgtt aagggatttt
     2761 ggtcatgaga ttatcaaaaa ggatcttcac ctagatcctt ttaaattaaa aatgaagttt
     2821 taaatcaatc taaagtatat atgagtaaac ttggtctgac agcggccgca aatgctaaac
     2881 cactgcagtg gttaccagtg cttgatcagt gaggcaccga tctcagcgat ctgcctattt
     2941 cgttcgtcca tagtggcctg actccccgtc gtgtagatca ctacgattcg tgagggctta
     3001 ccatcaggcc ccagcgcagc aatgatgccg cgagagccgc gttcaccggc ccccgatttg
     3061 tcagcaatga accagccagc agggagggcc gagcgaagaa gtggtcctgc tactttgtcc
     3121 gcctccatcc agtctatgag ctgctgtcgt gatgctagag taagaagttc gccagtgagt
     3181 agtttccgaa gagttgtggc cattgctact ggcatcgtgg tatcacgctc gtcgttcggt
     3241 atggcttcgt tcaactctgg ttcccagcgg tcaagccggg tcacatgatc acccatatta
     3301 tgaagaaatg cagtcagctc cttagggcct ccgatcgttg tcagaagtaa gttggccgcg
     3361 gtgttgtcgc tcatggtaat ggcagcacta cacaattctc ttaccgtcat gccatccgta
     3421 agatgctttt ccgtgaccgg cgagtactca accaagtcgt tttgtgagta gtgtatacgg
     3481 cgaccaagct gctcttgccc ggcgtctata cgggacaaca ccgcgccaca tagcagtact
     3541 ttgaaagtgc tcatcatcgg gaatcgttct tcggggcgga aagactcaag gatcttgccg
     3601 ctattgagat ccagttcgat atagcccact cttgcaccca gttgatcttc agcatctttt
     3661 actttcacca gcgtttcggg gtgtgcaaaa acaggcaagc aaaatgccgc aaagaaggga
     3721 atgagtgcga cacgaaaatg ttggatgctc atactcgtcc tttttcaata ttattgaagc
     3781 atttatcagg gttactagta cgtctctcaa ggataagtaa gtaatattaa ggtacgggag
     3841 gtattggaca ggccgcaata aaatatcttt attttcatta catctgtgtg ttggtttttt
     3901 gtgtgaatcg atagtactaa catacgctct ccatcaaaac aaaacgaaac aaaacaaact
     3961 agcaaaatag gctgtcccca gtgcaagtgc aggtgccaga acatttctct ggcctaactg
     4021 gccggtacct gagctcgcta gcctcgagga tatcctacac agagaaactc tttctcacaa
     4081 aaacaaatca tggaagactg aggatgtagt tcagtggtag aacgcttgct tgggcttgat
     4141 cgccattgcc acatccccaa ctgtttccgc tacacactca atagatctct ctttgacagc
     4201 tagaaagggc cgtcggaggc cccgccccgg aactgctggt acccggcccg ctcgtttcct
     4261 tcccagtgtc tcacagctta agcacaacaa atcggactgc agtccttggc aatccggtac
     4321 tgttggtaaa gccaccatgg aagatgccaa aaacattaag aagggcccag cgccattcta
     4381 cccactcgaa gacgggaccg ccggcgagca gctgcacaaa gccatgaagc gctacgccct
     4441 ggtgcccggc accatcgcct ttaccgacg
//
