LOCUS       sequence-35328-e         932 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 932)
  AUTHORS   Hussein R, Lim HN
  TITLE     Disruption of small RNA signaling caused by competition for Hfq.
  JOURNAL   Proc Natl Acad Sci U S A. 2011 Jan 18;108(3):1110-5. doi: 
            10.1073/pnas.1010082108. Epub 2010 Dec 28.
  PUBMED    21189298
REFERENCE   2  (bases 1 to 932)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1073/pnas.1010082108"; journalName: "Proc Natl Acad Sci U S A"; 
            date: "2011-01-18- 18"; volume: "108"; issue: "3"; pages: "1110-5"
COMMENT     Sequence Label: HL 2304 (=HL 716 + pHL 269)
FEATURES             Location/Qualifiers
     source          1..932
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     RBS             119..130
                     /note="strong bacterial ribosome binding site (Elowitz and 
                     Leibler, 2000)"
     protein_bind    283..301
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    308..326
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     terminator      complement(393..420)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(512..598)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     751..768
                     /label=pBAD Reverse
                     /note="For vectors with E. coli araBAD promoter, reverse 
                     primer"
     primer_bind     complement(854..874)
                     /label=GFP-F
                     /note="GFP, forward primer. Does NOT anneal to EGFP"
ORIGIN
        1 cctcgccctc gatctcgaac tcgtggccgt tcacggagcc ctccatgtgc accttgaagc
       61 gcatgaactc cttgatgatg gccatgttat cctcctcgcc cttgctcacc atcgtacgtt
      121 tctcctcttt aataagcttc tggataagga ttatccaatt ctaaaaaaaa agcccggacg
      181 actgttcggg cttgtctttt tatatgttgg aaaatcagtg gcaatgcaat ggcccaacag
      241 aaaataaaat ctgtgacaat aaaggcatat aacccgggag tatctctatc actgataggg
      301 atgtcaatct ctatcactga tagggaggat cctgagcgga tacatatttg aatgtattta
      361 gaaaaataaa caaaagagtt tgtagaaacg caaaaaggcc atccgtcagg atggccttct
      421 gcttaatttg atgcctggca gtttatggcg ggcgtcctgc ccgccaccct ccgggccgtt
      481 gcttcgcaac gttcaaatcc gctcccggcg gatttgtcct actcaggaga gcgttcaccg
      541 acaaacaaca gataaaacga aaggcccagt ctttcgactg agcctttcgt tttatttgat
      601 gcctggcagt tccctactct cgcatgggga gaccccacac taccatcggc gctacggcgt
      661 ttcacttctg agttcggcat ggggtcaggt gggaccaccg cgctactgcc gccaggcaaa
      721 ttctgtttta tcagaccgct tctgcgttct gatttaatct gtatcaggct gaaaatcttc
      781 tctcatccgc caaaacagcc aagctagctt ttatttgtag agctcatcca tgccatgtgt
      841 aatcccagca gctgttacaa actcaagaag gaccatgtgg tctctctttt cgttgggatc
      901 tttcgaaagg gcagattgtg tggacaggta at
//
