LOCUS       sequence-353280-        5592 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Luciferase reporter for mouse Tbp with Chantico promoter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5592)
  AUTHORS   Hall JM, Kainth AS, Rowton MJ, Lu E, Haddad G, Keplinger AJ, 
            Scepanovic J , Perez-Cervantes C, Chan SSK, Piccirilli JA, Moskowitz
            IP, Ruthenburg AJ
  TITLE     The cardiac lncRNA Chantico directly regulates Cxcl1 chemokine 
            transcription
  JOURNAL   bioRxiv 2021.12.23.474040
REFERENCE   2  (bases 1 to 5592)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "bioRxiv 
            2021.12.23.474040"
COMMENT     Sequence Label: pTbp-Firefly-Chantico
FEATURES             Location/Qualifiers
     source          1..5592
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     regulatory      346..355
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             352..2004
                     /codon_start=1
                     /product="firefly luciferase"
                     /label=luciferase
                     /note="synthetic luc2 version of the luciferase gene"
                     /translation="MEDAKNIKKGPAPFYPLEDGTAGEQLHKAMKRYALVPGTIAFTDA
                     HIEVDITYAEYFEMSVRLAEAMKRYGLNTNHRIVVCSENSLQFFMPVLGALFIGVAVAP
                     ANDIYNERELLNSMGISQPTVVFVSKKGLQKILNVQKKLPIIQKIIIMDSKTDYQGFQS
                     MYTFVTSHLPPGFNEYDFVPESFDRDKTIALIMNSSGSTGLPKGVALPHRTACVRFSHA
                     RDPIFGNQIIPDTAILSVVPFHHGFGMFTTLGYLICGFRVVLMYRFEEELFLRSLQDYK
                     IQSALLVPTLFSFFAKSTLIDKYDLSNLHEIASGGAPLSKEVGEAVAKRFHLPGIRQGY
                     GLTETTSAILITPEGDDKPGAVGKVVPFFEAKVVDLDTGKTLGVNQRGELCVRGPMIMS
                     GYVNNPEATNALIDKDGWLHSGDIAYWDEDEHFFIVDRLKSLIKYKGYQVAPAELESIL
                     LQHPNIFDAGVAGLPDDDAGELPAAVVVLEHGKTMTEKEIVDYVASQVTTAKKLRGGVV
                     FVDEVPKGLTGKLDARKIREILIKAKKGGKIAV"
     polyA_signal    2048..2169
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(2059..2078)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     2113..2132
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     complement(3540..3557)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(3711..4299)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(3791..3810)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(4499..5290,5291..5359))
                     /codon_start=1
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     polyA_signal    5464..5512
                     /note="synthetic polyadenylation signal"
     misc_feature    join(5526..5592,1..25)
                     /label=pause site
                     /note="RNA polymerase II transcriptional pause signal from 
                     the human alpha-2 globin gene"
     primer_bind     5566..5585
                     /label=RVprimer3
                     /note="pGL3 vector, forward primer"
ORIGIN
        1 agtgcaggtg ccagaacatt tctctggcct aactggccgg tacctgagct cgctagcctc
       61 gaggatatcc tacacagaga aactctttct cacaaaaaca aatcatggaa gactgaggat
      121 gtagttcagt ggtagaacgc ttgcttgggc ttgatcgcca ttgccacatc cccaactgtt
      181 tccgctacac actcaataga tctctctttg acagctagaa agggccgtcg gaggccccgc
      241 cccggaactg ctggtacccg gcccgctcgt ttccttccca gtgtctcaca gcttaagcac
      301 aacaaatcgg actgcagtcc ttggcaatcc ggtactgttg gtaaagccac catggaagat
      361 gccaaaaaca ttaagaaggg cccagcgcca ttctacccac tcgaagacgg gaccgccggc
      421 gagcagctgc acaaagccat gaagcgctac gccctggtgc ccggcaccat cgcctttacc
      481 gacgcacata tcgaggtgga cattacctac gccgagtact tcgagatgag cgttcggctg
      541 gcagaagcta tgaagcgcta tgggctgaat acaaaccatc ggatcgtggt gtgcagcgag
      601 aatagcttgc agttcttcat gcccgtgttg ggtgccctgt tcatcggtgt ggctgtggcc
      661 ccagctaacg acatctacaa cgagcgcgag ctgctgaaca gcatgggcat cagccagccc
      721 accgtcgtat tcgtgagcaa gaaagggctg caaaagatcc tcaacgtgca aaagaagcta
      781 ccgatcatac aaaagatcat catcatggat agcaagaccg actaccaggg cttccaaagc
      841 atgtacacct tcgtgacttc ccatttgcca cccggcttca acgagtacga cttcgtgccc
      901 gagagcttcg accgggacaa aaccatcgcc ctgatcatga acagtagtgg cagtaccgga
      961 ttgcccaagg gcgtagccct accgcaccgc accgcttgtg tccgattcag tcatgcccgc
     1021 gaccccatct tcggcaacca gatcatcccc gacaccgcta tcctcagcgt ggtgccattt
     1081 caccacggct tcggcatgtt caccacgctg ggctacttga tctgcggctt tcgggtcgtg
     1141 ctcatgtacc gcttcgagga ggagctattc ttgcgcagct tgcaagacta taagattcaa
     1201 tctgccctgc tggtgcccac actatttagc ttcttcgcta agagcactct catcgacaag
     1261 tacgacctaa gcaacttgca cgagatcgcc agcggcgggg cgccgctcag caaggaggta
     1321 ggtgaggccg tggccaaacg cttccaccta ccaggcatcc gccagggcta cggcctgaca
     1381 gaaacaacca gcgccattct gatcaccccc gaaggggacg acaagcctgg cgcagtaggc
     1441 aaggtggtgc ccttcttcga ggctaaggtg gtggacttgg acaccggtaa gacactgggt
     1501 gtgaaccagc gcggcgagct gtgcgtccgt ggccccatga tcatgagcgg ctacgttaac
     1561 aaccccgagg ctacaaacgc tctcatcgac aaggacggct ggctgcacag cggcgacatc
     1621 gcctactggg acgaggacga gcacttcttc atcgtggacc ggctgaagag cctgatcaaa
     1681 tacaagggct accaggtagc cccagccgaa ctggagagca tcctgctgca acaccccaac
     1741 atcttcgacg ccggggtcgc cggcctgccc gacgacgatg ccggcgagct gcccgccgca
     1801 gtcgtcgtgc tggaacacgg taaaaccatg accgagaagg agatcgtgga ctatgtggcc
     1861 agccaggtta caaccgccaa gaagctgcgc ggtggtgttg tgttcgtgga cgaggtgcct
     1921 aaaggactga ccggcaagtt ggacgcccgc aagatccgcg agattctcat taaggccaag
     1981 aagggcggca agatcgccgt gtaataattc tagagtcggg gcggccggcc gcttcgagca
     2041 gacatgataa gatacattga tgagtttgga caaaccacaa ctagaatgca gtgaaaaaaa
     2101 tgctttattt gtgaaatttg tgatgctatt gctttatttg taaccattat aagctgcaat
     2161 aaacaagtta acaacaacaa ttgcattcat tttatgtttc aggttcaggg ggaggtgtgg
     2221 gaggtttttt aaagcaagta aaacctctac aaatgtggta aaatcgataa ggatccggtc
     2281 attcagtaaa tataaatgca gatagcctac gttctgaaag tggggttcta gggtcccttg
     2341 gttagtttta aaaattcaaa gtcactttga gttttgtttt gttttgtttt gttttaaagc
     2401 ttgagagtgg ccgtgcagag caaagttgtc caattaagtt tatatcagtg acagatgggc
     2461 ttatcagacc ttcgctctca gacagtagcc atggaacact tagctgctgc ctcccgaaag
     2521 tgcaagcaga ccagttcagg atctgaagct ttgttttggt ttgacttaaa tgtaagtgca
     2581 aacagtcgct gtggggtgtg tgtgtgtgtg tgtgtgtgat actggccgat gcaggctgag
     2641 agtaggggtg tctcactcct gactgcgtac tccccctctg ttttacacag gcttatttac
     2701 tgttacacgg agacttttct tcgaactgtt aaactcttgg ctgagtttgt gtggagtatg
     2761 aggcaaatct ctaaccgcaa gcatgcagtg gaaagattag gcatactttt gaaacgagtt
     2821 tgtgtaccag atcatattct tggctcaaga aagaaaaaga aagggggctg gggaaaggct
     2881 tgtggttgct actctgggaa ctaaagaatt caaaaggctt gttctccatg tttcgtagac
     2941 acagacatct ggaattgttg ttttaaaatt acaagcaatt gtgtatgctt tgttactgca
     3001 acacttattc agaggcaagg acataggtgc cactgttgtc agaaagccag atgtcctttc
     3061 cttaaattgg cctgcctggc tgctggcgcc tgggtgtcag cagacacttt gggaatctca
     3121 gttagcggta ctgcatggat atagtgcatc tgcttctcac acttctgcca accttgtgta
     3181 ttggtttgct tctctccatg gtgttaaaac aatgactgaa agcgtcttgg gtagggcaag
     3241 ggtatattta gcttatagtt gcacatgaca gtccatcgtc aagggagagc atggcgggaa
     3301 ctcaaggact ttgaggagga actgaagcac aggcatagag gctgttcctc actggcttgc
     3361 tccccagggc ttgctcagct agctgtctta cagagcccag aaccccgatg cccttgagag
     3421 ccttcaaccc agtcagctcc ttccggtggg cgcggggcat gactatcgtc gccgcactta
     3481 tgactgtctt ctttatcatg caactcgtag gacaggtgcc ggcagcgctc ttccgcttcc
     3541 tcgctcactg actcgctgcg ctcggtcgtt cggctgcggc gagcggtatc agctcactca
     3601 aaggcggtaa tacggttatc cacagaatca ggggataacg caggaaagaa catgtgagca
     3661 aaaggccagc aaaaggccag gaaccgtaaa aaggccgcgt tgctggcgtt tttccatagg
     3721 ctccgccccc ctgacgagca tcacaaaaat cgacgctcaa gtcagaggtg gcgaaacccg
     3781 acaggactat aaagatacca ggcgtttccc cctggaagct ccctcgtgcg ctctcctgtt
     3841 ccgaccctgc cgcttaccgg atacctgtcc gcctttctcc cttcgggaag cgtggcgctt
     3901 tctcatagct cacgctgtag gtatctcagt tcggtgtagg tcgttcgctc caagctgggc
     3961 tgtgtgcacg aaccccccgt tcagcccgac cgctgcgcct tatccggtaa ctatcgtctt
     4021 gagtccaacc cggtaagaca cgacttatcg ccactggcag cagccactgg taacaggatt
     4081 agcagagcga ggtatgtagg cggtgctaca gagttcttga agtggtggcc taactacggc
     4141 tacactagaa gaacagtatt tggtatctgc gctctgctga agccagttac cttcggaaaa
     4201 agagttggta gctcttgatc cggcaaacaa accaccgctg gtagcggtgg tttttttgtt
     4261 tgcaagcagc agattacgcg cagaaaaaaa ggatctcaag aagatccttt gatcttttct
     4321 acggggtctg acgctcagtg gaacgaaaac tcacgttaag ggattttggt catgagatta
     4381 tcaaaaagga tcttcaccta gatcctttta aattaaaaat gaagttttaa atcaatctaa
     4441 agtatatatg agtaaacttg gtctgacagc ggccgcaaat gctaaaccac tgcagtggtt
     4501 accagtgctt gatcagtgag gcaccgatct cagcgatctg cctatttcgt tcgtccatag
     4561 tggcctgact ccccgtcgtg tagatcacta cgattcgtga gggcttacca tcaggcccca
     4621 gcgcagcaat gatgccgcga gagccgcgtt caccggcccc cgatttgtca gcaatgaacc
     4681 agccagcagg gagggccgag cgaagaagtg gtcctgctac tttgtccgcc tccatccagt
     4741 ctatgagctg ctgtcgtgat gctagagtaa gaagttcgcc agtgagtagt ttccgaagag
     4801 ttgtggccat tgctactggc atcgtggtat cacgctcgtc gttcggtatg gcttcgttca
     4861 actctggttc ccagcggtca agccgggtca catgatcacc catattatga agaaatgcag
     4921 tcagctcctt agggcctccg atcgttgtca gaagtaagtt ggccgcggtg ttgtcgctca
     4981 tggtaatggc agcactacac aattctctta ccgtcatgcc atccgtaaga tgcttttccg
     5041 tgaccggcga gtactcaacc aagtcgtttt gtgagtagtg tatacggcga ccaagctgct
     5101 cttgcccggc gtctatacgg gacaacaccg cgccacatag cagtactttg aaagtgctca
     5161 tcatcgggaa tcgttcttcg gggcggaaag actcaaggat cttgccgcta ttgagatcca
     5221 gttcgatata gcccactctt gcacccagtt gatcttcagc atcttttact ttcaccagcg
     5281 tttcggggtg tgcaaaaaca ggcaagcaaa atgccgcaaa gaagggaatg agtgcgacac
     5341 gaaaatgttg gatgctcata ctcgtccttt ttcaatatta ttgaagcatt tatcagggtt
     5401 actagtacgt ctctcaagga taagtaagta atattaaggt acgggaggta ttggacaggc
     5461 cgcaataaaa tatctttatt ttcattacat ctgtgtgttg gttttttgtg tgaatcgata
     5521 gtactaacat acgctctcca tcaaaacaaa acgaaacaaa acaaactagc aaaataggct
     5581 gtccccagtg ca
//
