LOCUS       sequence-353283-        3714 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses a truncated from of human Kir3.2 with a C-terminal 
            streptagII in insect cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3714)
  AUTHORS   Qiao P, Schrecke S, Walker T, McCabe JW, Lyu J, Zhu Y, Zhang T, 
            Kumar S, Clemmer D, Russell DH, Laganowsky A
  TITLE     Entropy in the Molecular Recognition of Membrane Protein-Lipid 
            Interactions.
  JOURNAL   J Phys Chem Lett. 2021 Dec 30;12(51):12218-12224. doi: 
            10.1021/acs.jpclett.1c03750. Epub 2021 Dec 20.
  PUBMED    34928154
REFERENCE   2  (bases 1 to 3714)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1021/acs.jpclett.1c03750"; journalName: "J Phys Chem Lett"; 
            date: "2021-12-30- 30"; volume: "12"; issue: "51"; pages: 
            "12218-12224"
COMMENT     Sequence Label: pACE-Truncated-Kir3.2-StreptagII
FEATURES             Location/Qualifiers
     source          1..3714
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        complement(join(80..85,86..102,103..108))
                     /gene="intI1 (promoter lies within the coding sequence)"
                     /label=Pc promoter
                     /note="class 1 integron promoter"
     protein_bind    complement(179..212)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     promoter        289..380
                     /gene="polh from Autographa californica"
                     /label=polyhedrin promoter
                     /note="promoter for the baculovirus polyhedrin gene"
     primer_bind     305..322
                     /label=Polyhedrin-F
                     /note="Polyhedrin promoter, forward primer"
     regulatory      385..394
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             1399..1422
                     /codon_start=1
                     /product="peptide that binds Strep-Tactin(R), an engineered
                     form of streptavidin"
                     /label=Strep-Tag II
                     /translation="WSHPQFEK"
     polyA_signal    1449..1583
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(1486..1505)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     1540..1559
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     mobile_element  1704..1869
                     /mobile_element_type="transposon:Tn7"
                     /label=Tn7L
                     /note="mini-Tn7 element (left end of the Tn7 transposon)"
     rep_origin      2059..2647
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     2548..2567
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     mobile_element  2782..3005
                     /mobile_element_type="transposon:Tn7"
                     /label=Tn7R
                     /note="mini-Tn7 element (right end of the Tn7 transposon)"
     CDS             complement(3072..3605)
                     /codon_start=1
                     /gene="aacC1"
                     /product="gentamycin acetyltransferase"
                     /label=GmR
                     /note="confers resistance to gentamycin"
                     /translation="MLRSSNDVTQQGSRPKTKLGGSSMGIIRTCRLGPDQVKSMRAALD
                     LFGREFGDVATYSQHQPDSDYLGNLLRSKTFIALAAFDQEAVVGALAAYVLPKFEQPRS
                     EIYIYDLAVSGEHRRQGIATALINLLKHEANALGAYVIYVQADYGDDPAVALYTKLGIR
                     EEVMHFDIDPSTAT"
ORIGIN
        1 cgccactgcg ccgttaccac cgctgcgttc ggtcaaggtt ctggaccagt tgcgtgagcg
       61 catacgctac ttgcattaca gtttacgaac cgaacaggct tatgtcaact gggttcgtgc
      121 cttcatccgt ttccacggtg tgcgtcaccc ggcaaccttg ggcagcagcg aagtcgccat
      181 aacttcgtat agcatacatt atacgaagtt atctgtaact ataacggtcc taaggtagcg
      241 agtttaaaca ctagtatcga ttcgcgacct actccggaat attaatagat catggagata
      301 attaaaatga taaccatctc gcaaataaat aagtatttta ctgttttcgt aacagttttg
      361 taataaaaaa acctataaat aaccgccacc atggccacca aaaggaaaat ccagaggtac
      421 gtgaggaaag acggaaagtg caatgttcat cacggcaacg tgagggagac ctatcgctac
      481 ctgaccgata tcttcaccac attagtggac ctgaagtgga gattcaacct attgattttt
      541 gtcatggttt acacagtgac ctggctcttt tttggaatga tctggtggtt gatcgcatac
      601 atacggggag acatggacca catagaggac ccctcctgga ctccttgtgt taccaacctc
      661 aacgggttcg tctctgcttt tttattctca atagagacag aaaccaccat tggttatggc
      721 taccgggtca tcacagataa atgcccggag ggaattattc ttctcttaat ccaatctgtg
      781 ttggggtcca ttgtcaatgc attcatggtg ggatgcatgt ttgtaaaaat ctctcaaccc
      841 aagaagaggg cagagaccct ggtcttttcc acccatgcag tgatctccat gcgggatggg
      901 aaactgtgcc tgatgttccg ggtaggggac cttaggaatt cccacattgt ggaggcttcc
      961 atcagagcca agttgatcaa atccaaacag acctcggagg gggagttcat cccgttgaac
     1021 cagacggata tcaacgtagg gtattacacg ggggatgacc gtctgtttct ggtgtcaccg
     1081 ctgatcatta gccatgaaat taaccaacag agtcctttct gggagatctc caaagcccag
     1141 ctgcccaaag aggaactgga aattgtggtc atcctagaag gaatggtgga agccacaggg
     1201 atgacatgcc aagctcgaag ctcctacatc accagtgaga tcctgtgggg ttaccggttc
     1261 acacctgtcc tgaccctgga ggacgggttc tacgaagttg actacaacag cttccatgag
     1321 acctatgaga ccagcacccc atcccttagt gccaaagagc tggccgagtt agccagcagg
     1381 gcagagggtg gagctagctg gtcacatcca cagtttgaga aataataatg aatgcaattg
     1441 ttgttgttaa cttgtttatt gcagcttata atggttacaa ataaagcaat agcatcacaa
     1501 atttcacaaa taaagcattt ttttcactgc attctagttg tggtttgtcc aaactcatca
     1561 atgtatctta tcatgtctgg atctgatcac tgcttgagcc tagaagatcc ggctgctaac
     1621 aaagcccgaa aggaagctga gttggctgct gccaccgctg agcaataact atcataaccc
     1681 ctagggtata cccatctaat tggaaccaga taagtgaaat ctagttccaa actattttgt
     1741 catttttaat tttcgtatta gcttacgacg ctacacccag ttcccatcta ttttgtcact
     1801 cttccctaaa taatccttaa aaactccatt tccacccctc ccagttccca actattttgt
     1861 ccgcccacaa ccggttgact tgggtcaact gtcagaccaa gtttactcat atatacttta
     1921 gattgattta aaacttcatt tttaatttaa aaggatctag gtgaagatcc tttttgataa
     1981 tctcatgacc aaaatccctt aacgtgagtt ttcgttccac tgagcgtcag accccgtaga
     2041 aaagatcaaa ggatcttctt gagatccttt ttttctgcgc gtaatctgct gcttgcaaac
     2101 aaaaaaacca ccgctaccag cggtggtttg tttgccggat caagagctac caactctttt
     2161 tccgaaggta actggcttca gcagagcgca gataccaaat actgttcttc tagtgtagcc
     2221 gtagttaggc caccacttca agaactctgt agcaccgcct acatacctcg ctctgctaat
     2281 cctgttacca gtggctgctg ccagtggcga taagtcgtgt cttaccgggt tggactcaag
     2341 acgatagtta ccggataagg cgcagcggtc gggctgaacg gggggttcgt gcacacagcc
     2401 cagcttggag cgaacgacct acaccgaact gagataccta cagcgtgagc tatgagaaag
     2461 cgccacgctt cccgaaggga gaaaggcgga caggtatccg gtaagcggca gggtcggaac
     2521 aggagagcgc acgagggagc ttccaggggg aaacgcctgg tatctttata gtcctgtcgg
     2581 gtttcgccac ctctgacttg agcgtcgatt tttgtgatgc tcgtcagggg ggcggagcct
     2641 atggaaaaac gccagcaacg cggccttttt acggttcctg gccttttgct ggccttttgc
     2701 tcacatgttc tttcctgcgt tatcccctga ttgacttggg tcgctcttcc tgtggatgcg
     2761 cagatgccct gcgtaagcgg gtgtgggcgg acaataaagt cttaaactga acaaaataga
     2821 tctaaactat gacaataaag tcttaaacta gacagaatag ttgtaaactg aaatcagtcc
     2881 agttatgctg tgaaaaagca tactggactt ttgttatggc taaagcaaac tcttcatttt
     2941 ctgaagtgca aattgcccgt cgtattaaag aggggcgtgg ccaagggcat gtaaagacta
     3001 tattcgcggc gttgtgacaa tttaccgaac aactccgcgg ccgggaagcc gatctcggct
     3061 tgaacgaatt gttaggtggc ggtacttggg tcgatatcaa agtgcatcac ttcttcccgt
     3121 atgcccaact ttgtatagag agccactgcg ggatcgtcac cgtaatctgc ttgcacgtag
     3181 atcacataag caccaagcgc gttggcctca tgcttgagga gattgatgag cgcggtggca
     3241 atgccctgcc tccggtgctc gccggagact gcgagatcat agatatagat ctcactacgc
     3301 ggctgctcaa acttgggcag aacgtaagcc gcgagagcgc caacaaccgc ttcttggtcg
     3361 aaggcagcaa gcgcgatgaa tgtcttacta cggagcaagt tcccgaggta atcggagtcc
     3421 ggctgatgtt gggagtaggt ggctacgtct ccgaactcac gaccgaaaag atcaagagca
     3481 gcccgcatgg atttgacttg gtcagggccg agcctacatg tgcgaatgat gcccatactt
     3541 gagccaccta actttgtttt agggcgactg ccctgctgcg taacatcgtt gctgctgcgt
     3601 aacatcgttg ctgctccata acatcaaaca tcgacccacg gcgtaacgcg cttgctgctt
     3661 ggatgcccga ggcatagact gtacaaaaaa acagtcataa caagccatga aaac
//
