LOCUS       sequence-353309-        9792 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Plasmid expressing optimized Cas9 and NAT marker. Compatible with 
            CTG-clade yeast species..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9792)
  AUTHORS   Strucko T, Andersen NL, Mahler MR, Martinez JL, Mortensen UH
  TITLE     A CRISPR/Cas9 method facilitates efficient oligo-mediated gene 
            editing in Debaryomyces hansenii.
  JOURNAL   Synth Biol (Oxf). 2021 Oct 12;6(1):ysab031. doi: 
            10.1093/synbio/ysab031. eCollection 2021.
  PUBMED    34746438
REFERENCE   2  (bases 1 to 9792)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Synth Biol 
            (Oxf)."; date: "2021-10-12"; volume: "6(1):ysab031. doi"; pages: " 
            10.1093/synbio/ysab031. eCollection 2021"
COMMENT     Sequence Label: pDIV491
FEATURES             Location/Qualifiers
     source          1..9792
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    12..515
                     /label=CEN/ARS
                     /note="S. cerevisiae CEN6 centromere fused to an 
                     autonomously replicating sequence"
     primer_bind     556..574
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     CDS             2058..6161
                     /codon_start=1
                     /product="Cas9 (Csn1) endonuclease from the Streptococcus 
                     pyogenes Type II CRISPR/Cas system"
                     /label=Cas9
                     /note="generates RNA-guided double strand breaks in DNA"
                     /translation="MDKKYSIGLDIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKK
                     NLIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEES
                     FLVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIK
                     FRGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRL
                     ENLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQ
                     IGDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVR
                     QQLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLL
                     RKQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARG
                     NSRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEY
                     FTVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFD
                     SVEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEER
                     LKTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFM
                     QLIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGR
                     HKPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLY
                     LYYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVP
                     SEEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKH
                     VAQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYL
                     NAVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKT
                     EITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSK
                     ESILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGI
                     TIMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNE
                     LALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILAD
                     ANLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVL
                     DATLIHQSITGLYETRIDLSQLGGD"
     CDS             2058..6161
                     /codon_start=1
                     /product="Cas9 (Csn1) endonuclease from the Streptococcus 
                     pyogenes Type II CRISPR/Cas system"
                     /label=Cas9
                     /note="generates RNA-guided double strand breaks in DNA"
                     /translation="MDKKYSIGLDIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKK
                     NLIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEES
                     FLVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIK
                     FRGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRL
                     ENLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQ
                     IGDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVR
                     QQLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLL
                     RKQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARG
                     NSRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEY
                     FTVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFD
                     SVEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEER
                     LKTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFM
                     QLIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGR
                     HKPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLY
                     LYYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVP
                     SEEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKH
                     VAQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYL
                     NAVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKT
                     EITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSK
                     ESILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGI
                     TIMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNE
                     LALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILAD
                     ANLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVL
                     DATLIHQSITGLYETRIDLSQLGGD"
     CDS             6174..6194
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             6198..6218
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             6222..6242
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             6243..6308
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
     primer_bind     complement(6389..6407)
                     /label=CYC1
                     /note="CYC1 transcription termination signal, reverse 
                     primer"
     terminator      6419..6607
                     /gene="S. cerevisiae CYC1"
                     /label=CYC1 terminator
                     /note="transcription terminator for CYC1"
     protein_bind    6647..6680
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     promoter        6735..7078
                     /label=TEF promoter
                     /note="Ashbya gossypii TEF promoter"
     terminator      7657..7854
                     /label=TEF terminator
                     /note="Ashbya gossypii TEF terminator"
     protein_bind    7917..7950
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     rep_origin      complement(8043..8631)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(8123..8142)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(8802..9593,9594..9662))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     9425..9444
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(9663..9767)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 gggtcctttt catcacgtgc tataaaaata attataattt aaatttttta atataaatat
       61 ataaattaaa aatagaaagt aaaaaaagaa attaaagaaa aaatagtttt tgttttccga
      121 agatgtaaaa gactctaggg ggatcgccaa caaatactac cttttatctt gctcttcctg
      181 ctctcaggta ttaatgccga attgtttcat cttgtctgtg tagaagacca cacacgaaaa
      241 tcctgtgatt ttacatttta cttatcgtta atcgaatgta tatctattta atctgctttt
      301 cttgtctaat aaatatatat gtaaagtacg ctttttgttg aaatttttta aacctttgtt
      361 tatttttttt tcttcattcc gtaactcttc taccttcttt atttactttc taaaatccaa
      421 atacaaaaca taaaaataaa taaacacaga gtaaattccc aaattattcc atcattaaaa
      481 gatacgaggc gcgtgtaagt tacaggcaag cgatccgtct aagaaaccat tattatcatg
      541 acattaacct ataaaaatag gcgtatcacg aggccctttg aatgcgtgcg atgggcttag
      601 ttagccttgc ttgtgacttt gcagctttat ccatgaacgg atcatacgat tgtatttctg
      661 aatttgtcat agtgacttta agttgttctt tatttggtat gattctattt tttaggactt
      721 tctaaaaaat caatttaaat tttggaaaac catttgtggt ttttcttaaa tgattgcaaa
      781 attattaaaa aatcgttaaa aaaatattca gtgtaaaaag actagcattc atgatctcaa
      841 catctttgga taatatcaga atgagaaaga acagatacgc agtacgtttt ttggtgagct
      901 ctttgcactt ctttagttct ttccatcaat atcagttttt taaactttta ggactaaaag
      961 tgatgtttaa cttcaaaatg tttaaaattt tgttcttccc gacgttcatt aagaatacta
     1021 atacacttta ataattattt taatattttg ttctaaataa tgacttttaa ttaaaaaaga
     1081 taaaatataa aaacatcata ataactcacc agaggttaag aacaaaaaaa caaattagat
     1141 atctgctaat ccaatatagt taaatcaatc tttccttggt ataatgggta tattacatat
     1201 atttcaagga ccgacactcc taccaaatat ctaaaattta ccatattaac ataacatgta
     1261 tataaacgtc aaatcataat cagcactagc ggaatgcgtg cgatcgcgtg cattcagcaa
     1321 ttccaaaatc ccattagagg ggttcttcac gattatctat ttgatcactc tcgcctaaac
     1381 tataaatata gctacctttt tatcaatata catgtacttc cgaacatcgg cccttatctg
     1441 ttattaatct attaataaac ccttaacaat ttcctgttgc tccttgtaaa ccagtcctat
     1501 atgtccaact tgcccggatt ggtctgatct tccgatctaa caatcacgtc ttactaatat
     1561 tataatgcct tggtaacacg tctcgcgttt acgcgtatgc cctttaggaa ttttgtaaac
     1621 aaactgcccc gtagattaca aacaagagag tgaattccac cctcaactat agacctagga
     1681 gccatttttc tatataacta ctatctgtat aaggatgggg cctcctggga catgttcaat
     1741 tgaattttac gcttcgaacc atacacataa cacgtaaaaa gctctaatat tgggcttctt
     1801 aaatggaaag ttgtagtata gattttacgc gatgatccaa ttcgcgtttt gtgtagggaa
     1861 ttgcttctac atcaagcaaa atttacaatg cttttatgta tacggccgta gacggaatag
     1921 acaacattgt ataatcagga attgatataa aatagttgat atcccactat tctaataact
     1981 tcatttttta ttaattagta tttgataata taaaatttca ttagttgctt tcaaacatta
     2041 ttcagacaat ctgtcatatg gataaaaagt atagtattgg tttagatatt ggtactaact
     2101 ctgtgggttg ggcagttatc actgacgaat ataaagttcc atcaaagaaa tttaaggtgt
     2161 taggtaacac tgacagacac tcaataaaaa agaatcttat cggtgctctt ttgttcgact
     2221 ccggtgaaac tgccgaggct acacgtttaa aaagaacagc aagaagaaga tatacccgta
     2281 gaaaaaatag aatatgttat ttacaagaaa tcttttctaa tgaaatggct aaagttgatg
     2341 attccttttt ccatagattg gaagagtcat ttttggttga agaagacaaa aagcatgaga
     2401 gacatccaat ctttgggaat atagttgatg aagtggctta ccatgaaaaa tatcctacca
     2461 tttatcattt aagaaagaaa ttggtagatt caactgataa agctgacctt agattaatct
     2521 atttagcact tgcccatatg attaaattta gaggtcattt tttgattgaa ggtgatttga
     2581 acccagataa ttctgacgtg gataaattat ttattcaatt agtccaaacc tacaaccaat
     2641 tatttgagga aaatccaatt aatgctagtg gtgtcgatgc caaagctata ttatcagcca
     2701 gattatcaaa atctagacgt ttggaaaatt tgattgccca attgccagga gaaaaaaaga
     2761 atggattatt tggaaacttg atcgcattat cattgggttt gacaccaaat tttaaatcta
     2821 attttgattt agctgaagat gctaaattac aattatcaaa agacacctat gacgacgatt
     2881 tggacaattt acttgctcaa attggtgatc aatatgcaga tttgttctta gctgctaaaa
     2941 acttatctga tgctattttg ttgtctgata ttttgagagt gaacacagaa ataaccaaag
     3001 ctccattatc agcatctatg atcaaacgtt atgatgaaca ccatcaggat ttgactttat
     3061 tgaaagcttt ggtgagacaa caattgccag agaagtataa agaaatcttt ttcgatcaat
     3121 ctaaaaacgg gtatgcaggt tatattgatg ggggtgcctc ccaagaggaa ttttacaaat
     3181 ttataaaacc tattttagaa aagatggatg ggactgagga acttttggtc aaattgaaca
     3241 gagaagattt gttacgtaaa cagagaactt ttgataatgg tagtatacct caccaaattc
     3301 atttgggtga gttgcatgca attttaagaa gacaagaaga tttttatcca tttttaaaag
     3361 ataatagaga aaaaatcgag aaaattttaa cctttagaat tccatactat gttgggcctt
     3421 tggctagagg taattcaaga tttgcctgga tgacacgtaa atcagaagaa actattaccc
     3481 cttggaattt tgaagaggtt gttgataaag gagcatcagc acagagtttt attgaaagaa
     3541 tgaccaattt cgataaaaac ttaccaaatg aaaaagtttt accaaaacat tccttgttat
     3601 acgaatattt tactgtttac aatgaactta caaaggttaa atatgttact gaaggtatgc
     3661 gtaagccagc ctttttatct ggagaacaga aaaaggcaat agttgattta ttgtttaaaa
     3721 caaatagaaa agttactgtt aaacaattaa aagaagatta ctttaagaaa attgaatgtt
     3781 ttgattcagt tgaaatcagt ggtgttgaag acagatttaa tgctagttta ggaacttacc
     3841 atgatttact taaaattatc aaagataaag atttcttgga taacgaagaa aatgaagaca
     3901 ttttagaaga cattgtttta accttaactt tattcgaaga tagagagatg attgaagaac
     3961 gtttgaagac ttatgcacat ttgtttgacg ataaagtgat gaaacagttg aaaagaagac
     4021 gttatactgg atggggtaga ttgtctcgta aattgatcaa tggaattaga gataaacaaa
     4081 gtggtaaaac tatcttggac tttttgaaat ctgacggatt tgctaataga aatttcatgc
     4141 aattgatcca cgacgatagt ttgacattta aagaagacat ccaaaaggcc caagtgagtg
     4201 ggcaaggtga ttcattacat gaacatattg caaatttagc cggatctcct gctattaaga
     4261 aagggatatt acaaactgtt aaagttgtgg atgaattagt gaaagtaatg ggaagacata
     4321 aacctgaaaa cattgtcatt gagatggcaa gagaaaatca aactacacaa aaaggacaga
     4381 aaaatagtag agaacgtatg aaaagaatag aagagggtat taaagaattg ggtagtcaaa
     4441 tattgaaaga acacccagtg gaaaataccc agttgcaaaa tgaaaaatta tatctttact
     4501 accttcaaaa tggacgtgat atgtatgttg atcaggaatt agatataaat agactttcag
     4561 attatgatgt agatcatata gttccacaat ctttcttgaa agatgattcc atagacaata
     4621 aagtattaac tagaagtgat aaaaatagag gtaaaagtga taatgtccca agtgaggaag
     4681 tcgtcaaaaa gatgaaaaat tactggcgtc aacttttgaa tgctaaatta attactcaaa
     4741 gaaaatttga taatttgact aaagcagaaa gaggtgggct ttctgaatta gataaagccg
     4801 ggttcattaa aagacaattg gtcgaaacta gacaaattac taaacatgtt gcccaaattt
     4861 tagattcccg tatgaacact aagtatgacg aaaatgataa gttaatacgt gaggttaaag
     4921 tcattacttt aaaatcaaaa cttgtctctg atttcagaaa ggatttccaa ttctataaag
     4981 ttagagaaat taataattat catcatgctc atgatgcata tttgaatgct gtagttggaa
     5041 ctgctttaat caagaaatac cctaaattag aatctgaatt tgtatatggt gattacaaag
     5101 tctatgatgt tagaaagatg attgctaaat cagaacaaga aattggtaaa gctacagcta
     5161 aatacttctt ttactctaac attatgaatt tctttaaaac agaaattact ttggcaaacg
     5221 gtgaaattag aaaaagacct cttattgaaa caaatggtga gactggagag atagtttggg
     5281 acaaagggcg tgatttcgct actgttagaa aagttttatc aatgccacaa gttaacattg
     5341 taaagaaaac agaggttcaa actggtggtt tctcaaaaga aagtattttg cctaaaagaa
     5401 atagtgataa attgattgcc agaaaaaagg attgggatcc aaagaaatat ggtggtttcg
     5461 actcaccaac cgtagcctat tctgttttgg ttgtggcaaa ggttgaaaag ggtaaaagta
     5521 aaaagcttaa atcagtaaaa gaacttttgg gtattacaat aatggaaaga agttcctttg
     5581 aaaagaaccc tattgatttt ttggaagcta aaggttataa ggaagtaaag aaggacttaa
     5641 taatcaaatt gcctaaatat tctttatttg aattagaaaa tgggagaaaa agaatgttgg
     5701 cttctgctgg agaattgcaa aagggtaatg aattagcatt gccttccaaa tatgttaact
     5761 tcttgtattt agcttcacac tatgaaaagt tgaaagggtc accagaagat aacgagcaaa
     5821 aacaattatt tgttgaacaa cacaaacact acttagatga gattatagaa caaattagtg
     5881 aattcagtaa aagagtgata ttagctgatg caaatttaga taaagttttg tcagcctata
     5941 acaaacatag agataagcca attagagaac aagcagaaaa cattattcac ttatttaccc
     6001 ttaccaattt aggagcacct gctgctttca agtattttga tacaacaatt gatcgtaaaa
     6061 gatatacctc aacaaaagaa gtcttagacg ccaccttaat tcatcaatca atcactggat
     6121 tgtatgagac aagaattgat ttgtctcaat tgggtggtga tgaaggggct gatcctaaga
     6181 agaaaagaaa agttgatcca aagaaaaagc gtaaggtgga tcctaagaaa aagagaaagg
     6241 ttgactacaa agaccatgac ggtgattata aagatcatga catcgactac aaggatgacg
     6301 atgacaagtg ataatgactg cagagatcca tcgacctgcc gccaagctaa ttccgggcga
     6361 atttctgtcg agtcatgtaa ttagttatgt cacgcttaca ttcacgccct ccccccacat
     6421 ccgctctaac cgaaaaggaa ggagttagac aacctgaagt ctaggtccct atttattttt
     6481 ttatagttat gttagtatta agaacgttat ttatatttca aatttttctt ttttttctgt
     6541 acagacgcgt gtacgcatgt aacattatac tgaaaacctt gcttgagaag gttttgggac
     6601 gctcgaaatc gcgtgcattc aacgctgcag gtcgacaacc cttaatataa cttcgtataa
     6661 tgtatgctat acgaagttat taggtctaga gatctgttta gcttgcctcg tccccgccgg
     6721 gtcacccggc cagcgacatg gaggcccaga ataccctcct tgacagtctt gacgtgcgca
     6781 gctcaggggc atgatgtgac tgtcgcccgt acatttagcc catacatccc catgtataat
     6841 catttgcatc catacatttt gatggccgca cggcgcgaag caaaaattac ggctcctcgc
     6901 tgcagacctg cgagcaggga aacgctcccc tcacagacgc gttgaattgt ccccacgccg
     6961 cgcccctgta gagaaatata aaaggttagg atttgccact gaggttcttc tttcatatac
     7021 ttccttttaa aatcttgcta ggatacagtt ctcacatcac atccgaacat aaacaaccat
     7081 gggaaccacc ttggatgaca ctgcttatcg ataccgaaca tctgtacctg gtgatgctga
     7141 agctatcgaa gcccttgacg ggtcattcac aactgacacc gtctttagag ttacagctac
     7201 tggggatggg tttacattgc gagaagtacc agtagatcct cctttgacca aagtattccc
     7261 agatgacgaa agtgacgatg aatccgatga cggtgaggac ggtgacccag actcaagaac
     7321 atttgtcgct tatggagacg acggtgattt ggccggtttc gtagttgtat cctattctgg
     7381 atggaaccga agacttactg tcgaggatat agaggttgcc ccagaacatc gtgggcacgg
     7441 tgttgggcga gcccttatgg gattagctac agaatttgcc cgtgaacgtg gggctggaca
     7501 tttatggtta gaagtcacca atgttaatgc accagctata catgcatatc gaagaatggg
     7561 gttcacactt tgtggattgg atacagcttt atacgatggt acagcctcag acggagaaca
     7621 agctttatac atgtctatgc cttgtccata atcagtactg acaataaaaa gattcttgtt
     7681 ttcaagaact tgtcatttgt atagtttttt tatattgtag ttgttctatt ttaatcaaat
     7741 gttagcgtga tttatatttt ttttcgcctc gacatcatct gcccagatgc gaagttaagt
     7801 gcgcagaaag taatatcatg cgttaatcgt atgtgaatgc tggtcgctat actgctgtcg
     7861 attcgatact aacgccgcca tccagtgtcg aaaacgagct ctcgagaacc cttaatataa
     7921 cttcgtataa tgtatgctat acgaagttat taggtgatat cagatccact agtggccatt
     7981 aagacctgag caaaaggcca gcaaaaggcc aggaaccgta aaaaggccgc gttgctggcg
     8041 tttttccata ggctccgccc ccctgacgag catcacaaaa atcgacgctc aagtcagagg
     8101 tggcgaaacc cgacaggact ataaagatac caggcgtttc cccctggaag ctccctcgtg
     8161 cgctctcctg ttccgaccct gccgcttacc ggatacctgt ccgcctttct cccttcggga
     8221 agcgtggcgc tttctcatag ctcacgctgt aggtatctca gttcggtgta ggtcgttcgc
     8281 tccaagctgg gctgtgtgca cgaacccccc gttcagcccg accgctgcgc cttatccggt
     8341 aactatcgtc ttgagtccaa cccggtaaga cacgacttat cgccactggc agcagccact
     8401 ggtaacagga ttagcagagc gaggtatgta ggcggtgcta cagagttctt gaagtggtgg
     8461 cctaactacg gctacactag aagaacagta tttggtatct gcgctctgct gaagccagtt
     8521 accttcggaa aaagagttgg tagctcttga tccggcaaac aaaccaccgc tggtagcggt
     8581 ggtttttttg tttgcaagca gcagattacg cgcagaaaaa aaggatctca agaagatcct
     8641 ttgatctttt ctacggggtc tgacgctcag tggaacgaaa actcacgtta agggattttg
     8701 gtcatgagat tatcaaaaag gatcttcacc tagatccttt taaattaaaa atgaagtttt
     8761 aaatcaatct aaagtatata tgagtaaact tggtctgaca gttaccaatg cttaatcagt
     8821 gaggcaccta tctcagcgat ctgtctattt cgttcatcca tagttgcctg actccccgtc
     8881 gtgtagataa ctacgatacg ggagggctta ccatctggcc ccagtgctgc aatgataccg
     8941 cgagacccac gctcaccggc tccagattta tcagcaataa accagccagc cggaagggcc
     9001 gagcgcagaa gtggtcctgc aactttatcc gcctccatcc agtctattaa ttgttgccgg
     9061 gaagctagag taagtagttc gccagttaat agtttgcgca acgttgttgc cattgctaca
     9121 ggcatcgtgg tgtcacgctc gtcgtttggt atggcttcat tcagctccgg ttcccaacga
     9181 tcaaggcgag ttacatgatc ccccatgttg tgcaaaaaag cggttagctc cttcggtcct
     9241 ccgatcgttg tcagaagtaa gttggccgca gtgttatcac tcatggttat ggcagcactg
     9301 cataattctc ttactgtcat gccatccgta agatgctttt ctgtgactgg tgagtactca
     9361 accaagtcat tctgagaata gtgtatgcgg cgaccgagtt gctcttgccc ggcgtcaata
     9421 cgggataata ccgcgccaca tagcagaact ttaaaagtgc tcatcattgg aaaacgttct
     9481 tcggggcgaa aactctcaag gatcttaccg ctgttgagat ccagttcgat gtaacccact
     9541 cgtgcaccca actgatcttc agcatctttt actttcacca gcgtttctgg gtgagcaaaa
     9601 acaggaaggc aaaatgccgc aaaaaaggga ataagggcga cacggaaatg ttgaatactc
     9661 atactcttcc tttttcaata ttattgaagc atttatcagg gttattgtct catgagcgga
     9721 tacatatttg aatgtattta gaaaaataaa caaatagggg ttccgcgcac atttccccga
     9781 aaagtgccac ct
//
