LOCUS       sequence-35331-e         822 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 822)
  AUTHORS   Hussein R, Lim HN
  TITLE     Disruption of small RNA signaling caused by competition for Hfq.
  JOURNAL   Proc Natl Acad Sci U S A. 2011 Jan 18;108(3):1110-5. doi: 
            10.1073/pnas.1010082108. Epub 2010 Dec 28.
  PUBMED    21189298
REFERENCE   2  (bases 1 to 822)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1073/pnas.1010082108"; journalName: "Proc Natl Acad Sci U S A"; 
            date: "2011-01-18- 18"; volume: "108"; issue: "3"; pages: "1110-5"
COMMENT     Sequence Label: HL 1474 (=HL 862 + pHL 404)
FEATURES             Location/Qualifiers
     source          1..822
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    148..166
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    173..191
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     primer_bind     complement(212..230)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        298..402
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(621..640)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
ORIGIN
        1 tcttctcctt tacgcatgct agctgggacc aggagggaca gtactttaac tttcatgtta
       61 ttaaccctct gttatatgcc tttatttgct tttttatgcc actgcatact gattaaccct
      121 cattaatcag tcggcaagtc gacagtatct ctatcactga tagggatgtc aatctctatc
      181 actgataggg actcgaggtg aagacgaaag ggcctcgtga tacgcctatt tttataggtt
      241 aatgtcatga taataatggt ttcttagacg tcaggtggca cttttcgggg aaatgtgcgc
      301 ggaaccccta tttgtttatt tttctaaata cattcaaata tgtatccgct catgagacaa
      361 taaccctgat aaatgcttca ataatattga aaaaggaaga gtatgagtat tcaacatttc
      421 cgtgtcgccc ttattccctt ttttgcggca ttttgccttc ctgtttttgc tcacccagaa
      481 acgctggtga aagtaaaaga tgctgaagat cagttgggtg cacgagtggg ttacatcgaa
      541 ctggatctca acagcggtaa gatccttgag agttttcgcc ccgaagaacg ttttccaatg
      601 atgagcactt ttaaagttct gctatgtggc gcggtattat cccgtattga cgccgggcaa
      661 gagcaactcg gtcgccgcat acactattct cagaatgact tggttgagta ctcaccagtc
      721 acagaaaagc atcttacgga tggcatgaca gtaagagaat tatgcagtgc tgccataacc
      781 atgagtgata acactgcggc caacttactt ctgacaacga tc
//
