LOCUS       sequence-353328-       10711 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10711)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 10711)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF2504
FEATURES             Location/Qualifiers
     source          1..10711
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        1..1179
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          232..1170
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     1127..1147
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     regulatory      1190..1199
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     misc_feature    3649..4237
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(3702..3722)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(4120..4131)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             4309..4542
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    4620..4741
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(4657..4676)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     4711..4730
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      4781..4916
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(4936..4955)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(4937..4955)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(4965..4982)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(4965..4981)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(4974..4996)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      5123..5578
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(5210..5229)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     5420..5441
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        5604..5708
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(5709..5777,5778..6569)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(5927..5946)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      6740..7328
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     7229..7248
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     7482..7499
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    7616..7637
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(7652..7657,7658..7675,7676..7682)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7690..7706
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     7695..7717
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     7714..7730
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     7714..7730
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     7749..7769
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        7751..7769
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        7797..8023
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             8024..8204
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    8251..8376
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    8869..9102
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             9287..9331
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     promoter        9562..9772
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     promoter        9584..9780
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     rep_origin      9631..9766
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             9812..10414
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGAS"
     primer_bind     complement(9812..9831)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     10308..10328
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     misc_feature    10457..10574
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
ORIGIN
        1 ggctccggtg cccgtcagtg ggcagagcgc acatcgccca cagtccccga gaagttgggg
       61 ggaggggtcg gcaattgaac cggtgcctag agaaggtggc gcggggtaaa ctgggaaagt
      121 gatgtcgtgt actggctccg cctttttccc gagggtgggg gagaaccgta tataagtgca
      181 gtagtcgccg tgaacgttct ttttcgcaac gggtttgccg ccagaacaca ggtaagtgcc
      241 gtgtgtggtt cccgcgggcc tggcctcttt acgggttatg gcccttgcgt gccttgaatt
      301 acttccacct ggctgcagta cgtgattctt gatcccgagc ttcgggttgg aagtgggtgg
      361 gagagttcga ggccttgcgc ttaaggagcc ccttcgcctc gtgcttgagt tgaggcctgg
      421 cctgggcgct ggggccgccg cgtgcgaatc tggtggcacc ttcgcgcctg tctcgctgct
      481 ttcgataagt ctctagccat ttaaaatttt tgatgacctg ctgcgacgct ttttttctgg
      541 caagatagtc ttgtaaatgc gggccaagat ctgcacactg gtatttcggt ttttggggcc
      601 gcgggcggcg acggggcccg tgcgtcccag cgcacatgtt cggcgaggcg gggcctgcga
      661 gcgcggccac cgagaatcgg acgggggtag tctcaagctg gccggcctgc tctggtgcct
      721 ggcctcgcgc cgccgtgtat cgccccgccc tgggcggcaa ggctggcccg gtcggcacca
      781 gttgcgtgag cggaaagatg gccgcttccc ggccctgctg cagggagctc aaaatggagg
      841 acgcggcgct cgggagagcg ggcgggtgag tcacccacac aaaggaaaag ggcctttccg
      901 tcctcagccg tcgcttcatg tgactccacg gagtaccggg cgccgtccag gcacctcgat
      961 tagttctcga gcttttggag tacgtcgtct ttaggttggg gggaggggtt ttatgcgatg
     1021 gagtttcccc acactgagtg ggtggagact gaagttaggc cagcttggca cttgatgtaa
     1081 ttctccttgg aatttgccct ttttgagttt ggatcttggt tcattctcaa gcctcagaca
     1141 gtggttcaaa gtttttttct tccatttcag gtgtcgtgag gctagcaccg ccaccatgga
     1201 accggcgcca gcgcgtagtc cacgtccaca gcaagatcca gcccgcccgc aagaaccaac
     1261 gatgccacca ccagaaaccc caagcgaagg ccgccagcca agcccgagtc caagtccgac
     1321 ggaacgtgcc ccggcgagtg aagaggaatt ccagtttctg cgctgccagc agtgtcaagc
     1381 cgaagccaaa tgcccgaaac tgctgccatg tctgcatacg ctgtgcagcg gttgtctgga
     1441 agccagtggt atgcagtgcc caatttgcca agccccgtgg ccactgggcg ccgatacgcc
     1501 agccctcgac aacgtgtttt tcgaaagtct gcagcgtcgt ctgagcgttt accgccagat
     1561 tgtggacgcc caagccgttt gcacccgttg caaggaaagt gcggactttt ggtgcttcga
     1621 gtgcgaacag ctgctgtgcg ccaaatgctt tgaagcgcac cagtggtttc tgaaacacga
     1681 agcccgtcca ctggcggaac tgcgcaacca gagcgttcgt gagtttctgg atggcacccg
     1741 caagaccaac aatatctttt gcagtaaccc gaatcaccgc acgccgacgc tgaccagtat
     1801 ctactgccgc ggctgcagca aaccactgtg ctgcagctgt gcgctgctgg atagcagcca
     1861 cagcgaactg aagtgcgaca tcagtgcgga aatccagcaa cgccaagaag aactcgatgc
     1921 gatgacccaa gcgctgcaag aacaagatag tgcgtttggt gccgttcatg cccagatgca
     1981 tgcggcggtt ggtcaactgg gccgtgcccg cgccgaaacc gaagaactga tccgtgagcg
     2041 cgtgcgccaa gttgtggccc atgttcgcgc ccaagaacgc gaactgctgg aagcggttga
     2101 tgcgcgctac cagcgtgatt atgaagagat ggccagccgt ctgggtcgtc tggatgcggt
     2161 gctgcagcgc attcgcaccg gtagcgcgct ggtgcagcgc atgaaatgct atgcgagcga
     2221 ccaagaagtg ctggatatgc acggttttct gcgtcaagcg ctgtgccgtc tgcgccaaga
     2281 ggaaccacag agtctgcaag ccgccgtgcg taccgatggc ttcgacgaat tcaaggttcg
     2341 tctgcaagat ctgagcagct gcatcaccca aggcaaagat gccgcggtga gcaaaaaagc
     2401 gagcccagaa gccgccagta cccccaggga ccctattgac gttgacctgg atgtctccaa
     2461 tacaacgaca gcccagaaga ggaagtgcag ccagacccag tgccccagga aggtcatcaa
     2521 gatggagtct gaggagggga aggaggcaag gttggctcgg agctccccgg agcagcccag
     2581 gcccagcacc tccaaggcag tctcaccacc ccacctggat ggaccgccta gccccaggag
     2641 ccccgtcata ggaagtgagg tcttcctgcc caacagcaac cacgtggcca gtggcgccgg
     2701 ggaggcagag gaacgcgttg tggtgatcag cagctcggaa gactcagatg ccgaaaactc
     2761 gtgcatggag cccatggaga ccgccgagcc acagtcctcg ccagcccact cctcgccagc
     2821 ccactcctcg ccagcccact cctcgccagt ccagtctctg ctgagagcac aaggagcctc
     2881 cagcctgccc tgtggcacat accacccccc agcttggcct ccccaccagc ccgctgagca
     2941 ggctgccacc cccgatgctg agcctcacag cgagcctcct gatcaccagg agcgccctgc
     3001 cgtccaccgt gggatccgct acctgttgta cagagcacag agagccatcc gccttcgcca
     3061 tgccctccgc ttgcaccctc aattgcatcg ggcccctatt cggacttggt ctccccatgt
     3121 ggtccaagcc agcactcctg ccatcacagg gcccctcaac catcctgcca atgcccagga
     3181 acatcctgcc cagctgcaaa ggggcatcag cccaccccac cggatacgag gggctgtgcg
     3241 atcccgcagc cgctccctcc ggggctcctc ccatttatcc cagtggctca acaacttttt
     3301 tgccctcccc ttctcctcca tggcttccca gcttgacatg tcttccgtgg tgggggcagg
     3361 ggaaagcaga gcccagactc ttggagcagg tgttccccct ggggactctg tcagaggctc
     3421 catggaggcc tctcaagtcc aagtgcctct ggaagcctct ccaattacat tcccaccacc
     3481 ctgtgcccca gaaaggcccc ccatcagccc agtcccaggc gcccgtcaag caggcctcta
     3541 gtaatgaact agtccgtaac ttgaaagtat ttcgatttct tggctttata tatcttgtgg
     3601 aaaggacgat agcattctag caatcccttg agtacgcgtt aagtcgacaa tcaacctctg
     3661 gattacaaaa tttgtgaaag attgactggt attcttaact atgttgctcc ttttacgcta
     3721 tgtggatacg ctgctttaat gcctttgtat catgctattg cttcccgtat ggctttcatt
     3781 ttctcctcct tgtataaatc ctggttgctg tctctttatg aggagttgtg gcccgttgtc
     3841 aggcaacgtg gcgtggtgtg cactgtgttt gctgacgcaa cccccactgg ttggggcatt
     3901 gccaccacct gtcagctcct ttccgggact ttcgctttcc ccctccctat tgccacggcg
     3961 gaactcatcg ccgcctgcct tgcccgctgc tggacagggg ctcggctgtt gggcactgac
     4021 aattccgtgg tgttgtcggg gaaatcatcg tcctttcctt ggctgctcgc ctgtgttgcc
     4081 acctggattc tgcgcgggac gtccttctgc tacgtccctt cggccctcaa tccagcggac
     4141 cttccttccc gcggcctgct gccggctctg cggcctcttc cgcgtcttcg ccttcgccct
     4201 cagacgagtc ggatctccct ttgggccgcc tccccgcgtc gactttaaga ccaatgactt
     4261 acaaggcagc tgtagatctt agccactttt taaaagaaaa ggggggactg gaagggctaa
     4321 ttcactccca acgaagacaa gatctgcttt ttgcttgtac tgggtctctc tggttagacc
     4381 agatctgagc ctgggagctc tctggctaac tagggaaccc actgcttaag cctcaataaa
     4441 gcttgccttg agtgcttcaa gtagtgtgtg cccgtctgtt gtgtgactct ggtaactaga
     4501 gatccctcag acccttttag tcagtgtgga aaatctctag cagtacgtat agtagttcat
     4561 gtcatcttat tattcagtat ttataacttg caaagaaatg aatatcagag agtgagagga
     4621 acttgtttat tgcagcttat aatggttaca aataaagcaa tagcatcaca aatttcacaa
     4681 ataaagcatt tttttcactg cattctagtt gtggtttgtc caaactcatc aatgtatctt
     4741 atcatgtctg gctctagcta tcccgcccct aactccgccc atcccgcccc taactccgcc
     4801 cagttccgcc cattctccgc cccatggctg actaattttt tttatttatg cagaggccga
     4861 ggccgcctcg gcctctgagc tattccagaa gtagtgagga ggcttttttg gaggcctagg
     4921 gacgtaccca attcgcccta tagtgagtcg tattacgcgc gctcactggc cgtcgtttta
     4981 caacgtcgtg actgggaaaa ccctggcgtt acccaactta atcgccttgc agcacatccc
     5041 cctttcgcca gctggcgtaa tagcgaagag gcccgcaccg atcgcccttc ccaacagttg
     5101 cgcagcctga atggcgaatg ggacgcgccc tgtagcggcg cattaagcgc ggcgggtgtg
     5161 gtggttacgc gcagcgtgac cgctacactt gccagcgccc tagcgcccgc tcctttcgct
     5221 ttcttccctt cctttctcgc cacgttcgcc ggctttcccc gtcaagctct aaatcggggg
     5281 ctccctttag ggttccgatt tagtgcttta cggcacctcg accccaaaaa acttgattag
     5341 ggtgatggtt cacgtagtgg gccatcgccc tgatagacgg tttttcgccc tttgacgttg
     5401 gagtccacgt tctttaatag tggactcttg ttccaaactg gaacaacact caaccctatc
     5461 tcggtctatt cttttgattt ataagggatt ttgccgattt cggcctattg gttaaaaaat
     5521 gagctgattt aacaaaaatt taacgcgaat tttaacaaaa tattaacgct tacaatttag
     5581 gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt
     5641 caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa
     5701 ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt
     5761 gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt
     5821 tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt
     5881 ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg
     5941 tattatcccg tattgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga
     6001 atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa
     6061 gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga
     6121 caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa
     6181 ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca
     6241 ccacgatgcc tgtagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta
     6301 ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac
     6361 ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc
     6421 gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag
     6481 ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga
     6541 taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt
     6601 agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata
     6661 atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag
     6721 aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa
     6781 caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt
     6841 ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgttctt ctagtgtagc
     6901 cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa
     6961 tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa
     7021 gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc
     7081 ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa
     7141 gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa
     7201 caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg
     7261 ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc
     7321 tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg
     7381 ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg
     7441 agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg
     7501 aagcggaaga gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg attcattaat
     7561 gcagctggca cgacaggttt cccgactgga aagcgggcag tgagcgcaac gcaattaatg
     7621 tgagttagct cactcattag gcaccccagg ctttacactt tatgcttccg gctcgtatgt
     7681 tgtgtggaat tgtgagcgga taacaatttc acacaggaaa cagctatgac catgattacg
     7741 ccaagcgcgc aattaaccct cactaaaggg aacaaaagct ggagctgcaa gcttaatgta
     7801 gtcttatgca atactcttgt agtcttgcaa catggtaacg atgagttagc aacatgcctt
     7861 acaaggagag aaaaagcacc gtgcatgccg attggtggaa gtaaggtggt acgatcgtgc
     7921 cttattagga aggcaacaga cgggtctgac atggattgga cgaaccactg aattgccgca
     7981 ttgcagagat attgtattta agtgcctagc tcgatacata aacgggtctc tctggttaga
     8041 ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata
     8101 aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta
     8161 gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtggcg cccgaacagg
     8221 gacttgaaag cgaaagggaa accagaggag ctctctcgac gcaggactcg gcttgctgaa
     8281 gcgcgcacgg caagaggcga ggggcggcga ctggtgagta cgccaaaaat tttgactagc
     8341 ggaggctaga aggagagaga tgggtgcgag agcgtcagta ttaagcgggg gagaattaga
     8401 tcgcgatggg aaaaaattcg gttaaggcca gggggaaaga aaaaatataa attaaaacat
     8461 atagtatggg caagcaggga gctagaacga ttcgcagtta atcctggcct gttagaaaca
     8521 tcagaaggct gtagacaaat actgggacag ctacaaccat cccttcagac aggatcagaa
     8581 gaacttagat cattatataa tacagtagca accctctatt gtgtgcatca aaggatagag
     8641 ataaaagaca ccaaggaagc tttagacaag atagaggaag agcaaaacaa aagtaagacc
     8701 accgcacagc aagcggccgc tgatcttcag acctggagga ggagatatga gggacaattg
     8761 gagaagtgaa ttatataaat ataaagtagt aaaaattgaa ccattaggag tagcacccac
     8821 caaggcaaag agaagagtgg tgcagagaga aaaaagagca gtgggaatag gagctttgtt
     8881 ccttgggttc ttgggagcag caggaagcac tatgggcgca gcgtcaatga cgctgacggt
     8941 acaggccaga caattattgt ctggtatagt gcagcagcag aacaatttgc tgagggctat
     9001 tgaggcgcaa cagcatctgt tgcaactcac agtctggggc atcaagcagc tccaggcaag
     9061 aatcctggct gtggaaagat acctaaagga tcaacagctc ctggggattt ggggttgctc
     9121 tggaaaactc atttgcacca ctgctgtgcc ttggaatgct agttggagta ataaatctct
     9181 ggaacagatt tggaatcaca cgacctggat ggagtgggac agagaaatta acaattacac
     9241 aagcttaata cactccttaa ttgaagaatc gcaaaaccag caagaaaaga atgaacaaga
     9301 attattggaa ttagataaat gggcaagttt gtggaattgg tttaacataa caaattggct
     9361 gtggtatata aaattattca taatgatagt aggaggcttg gtaggtttaa gaatagtttt
     9421 tgctgtactt tctatagtga atagagttag gcagggatat tcaccattat cgtttcagac
     9481 ccacctccca accccgaggg gacccatgca ttgcatctca attagtcagc aaccaggtgt
     9541 ggaaagtccc caggctcccc agcaggcaga agtatgcaaa gcatgcgtct caattagtca
     9601 gcaaccatag tcccgcccct aactccgccc atcccgcccc taactccgcc cagttccgcc
     9661 cattctccgc cccatggctg actaattttt tttatttatg cagaggccga ggccgcctcg
     9721 gcctctgagc tattccagaa gtagtgagga ggcttttttg gaggcctagg cttttgcaaa
     9781 aagctttcta gaggtaccac cggagcttac catgaccgag tacaagccca cggtgcgcct
     9841 cgccacccgc gacgacgtcc ccagggccgt acgcaccctc gccgccgcgt tcgccgacta
     9901 ccccgccacg cgccacaccg tcgatccgga ccgccacatc gagcgggtca ccgagctgca
     9961 agaactcttc ctcacgcgcg tcgggctcga catcggcaag gtgtgggtcg cggacgacgg
    10021 cgccgccgtg gcggtctgga ccacgccgga gagcgtcgaa gcgggggcgg tgttcgccga
    10081 gatcggcccg cgcatggccg agttgagcgg ttcccggctg gccgcgcagc aacagatgga
    10141 aggcctcctg gcgccgcacc ggcccaagga gcccgcgtgg ttcctggcca ccgtcggcgt
    10201 ctcgcccgac caccagggca agggtctggg cagcgccgtc gtgctccccg gagtggaggc
    10261 ggccgagcgc gccggggtgc ccgccttcct ggagacctcc gcgccccgca acctcccctt
    10321 ctacgagcgg ctcggcttca ccgtcaccgc cgacgtcgag gtgcccgaag gaccgcgcac
    10381 ctggtgcatg acccgcaagc ccggtgccag ttaacgaatt ctagatcttg agacaaatgg
    10441 cagtattcat ccacaatttt aaaagaaaag gggggattgg ggggtacagt gcaggggaaa
    10501 gaatagtaga cataatagca acagacatac aaactaaaga attacaaaaa caaattacaa
    10561 aaattcaaaa ttttcgggtt tattacaggg acagcagaga tccactttgg cgccggctcg
    10621 aggcctgcag gtgcaaagat ggataaagtt ttaaacagag aggaatcttt gcagctaatg
    10681 gaccttctag gtcttgaaag gagtgggaat t
//
