LOCUS       sequence-353329-       13192 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 13192)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 13192)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF2640
FEATURES             Location/Qualifiers
     source          1..13192
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        1..1179
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          232..1170
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     1127..1147
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     regulatory      3200..3209
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             complement(4237..4254)
                     /codon_start=1
                     /product="thrombin recognition and cleavage site"
                     /label=thrombin site
                     /translation="LVPRGS"
     misc_feature    6130..6718
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(6183..6203)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(6601..6612)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             6790..7023
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    7101..7222
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(7138..7157)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     7192..7211
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      7262..7397
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(7417..7436)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(7418..7436)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(7446..7463)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(7446..7462)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7455..7477)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      7604..8059
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(7691..7710)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     7901..7922
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        8085..8189
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(8190..8258,8259..9050)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(8408..8427)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      9221..9809
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     9710..9729
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     9963..9980
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    10097..10118
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(10133..10138,10139..10156,10157..10163)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    10171..10187
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     10176..10198
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     10195..10211
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     10195..10211
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     10230..10250
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        10232..10250
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        10278..10504
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             10505..10685
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    10732..10857
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    11350..11583
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             11768..11812
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     promoter        12043..12253
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     promoter        12065..12261
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     rep_origin      12112..12247
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             12293..12895
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGAS"
     primer_bind     complement(12293..12312)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     12789..12809
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     misc_feature    12938..13055
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
ORIGIN
        1 ggctccggtg cccgtcagtg ggcagagcgc acatcgccca cagtccccga gaagttgggg
       61 ggaggggtcg gcaattgaac cggtgcctag agaaggtggc gcggggtaaa ctgggaaagt
      121 gatgtcgtgt actggctccg cctttttccc gagggtgggg gagaaccgta tataagtgca
      181 gtagtcgccg tgaacgttct ttttcgcaac gggtttgccg ccagaacaca ggtaagtgcc
      241 gtgtgtggtt cccgcgggcc tggcctcttt acgggttatg gcccttgcgt gccttgaatt
      301 acttccacct ggctgcagta cgtgattctt gatcccgagc ttcgggttgg aagtgggtgg
      361 gagagttcga ggccttgcgc ttaaggagcc ccttcgcctc gtgcttgagt tgaggcctgg
      421 cctgggcgct ggggccgccg cgtgcgaatc tggtggcacc ttcgcgcctg tctcgctgct
      481 ttcgataagt ctctagccat ttaaaatttt tgatgacctg ctgcgacgct ttttttctgg
      541 caagatagtc ttgtaaatgc gggccaagat ctgcacactg gtatttcggt ttttggggcc
      601 gcgggcggcg acggggcccg tgcgtcccag cgcacatgtt cggcgaggcg gggcctgcga
      661 gcgcggccac cgagaatcgg acgggggtag tctcaagctg gccggcctgc tctggtgcct
      721 ggcctcgcgc cgccgtgtat cgccccgccc tgggcggcaa ggctggcccg gtcggcacca
      781 gttgcgtgag cggaaagatg gccgcttccc ggccctgctg cagggagctc aaaatggagg
      841 acgcggcgct cgggagagcg ggcgggtgag tcacccacac aaaggaaaag ggcctttccg
      901 tcctcagccg tcgcttcatg tgactccacg gagtaccggg cgccgtccag gcacctcgat
      961 tagttctcga gcttttggag tacgtcgtct ttaggttggg gggaggggtt ttatgcgatg
     1021 gagtttcccc acactgagtg ggtggagact gaagttaggc cagcttggca cttgatgtaa
     1081 ttctccttgg aatttgccct ttttgagttt ggatcttggt tcattctcaa gcctcagaca
     1141 gtggttcaaa gtttttttct tccatttcag gtgtcgtgag gctagcaccg ccaccatgta
     1201 ttcggcccac aggcccctga tgcccgcgtc cagcgcggcc tcccgtggcc tcggcatgtt
     1261 cgtgtggacg aatgtggaac ctcgctctgt ggctgtgttc ccttggcact ccttagtccc
     1321 cttcctggca cccagccagc ctgacccctc cgtgcagccg agcgaggccc agcaacctgc
     1381 cagccaccca gtggcctcca accagagcaa agaacctgct gagtcggcag ctgttgctca
     1441 tgaacggcca ccaggtggga cagggagtgc tgaccctgag cggccccctg gagccacatg
     1501 ccctgagagc ccaggacccg gacccccaca ccctttgggg gtggtggaat ctggtaaggg
     1561 tccgcctccc accacggagg aggaggcctc cggcccccca ggagagcccc ggctggacag
     1621 tgagacagag agtgaccatg atgatgcctt cctctccatc atgtctcctg agatccagtt
     1681 gcctctaccg cccggaaaac gtcggaccca gtccctcagt gccctaccca aggaacggga
     1741 ctcatcttct gagaaggatg gacgcagccc caacaagcgg gagaaggacc acatccggcg
     1801 gcccatgaat gccttcatga tcttcagcaa gcggcaccgg gccctggtcc accagcgtca
     1861 tcccaaccag gacaaccgga ccgtcagcaa gatcctgggc gagtggtggt atgccctggg
     1921 gcccaaggag aagcagaagt accacgacct ggccttccag gtgaaggagg cccacttcaa
     1981 ggcccaccca gattggaagt ggtgcaacaa ggaccgaaag aagtccagct cagaggccaa
     2041 gcccacgagc ctggggctgg caggagggca caaggagacg cgggagcgga gcatgtcgga
     2101 gacgggcact gctgctgccc ctggggtgtc ctctgagctc ctgtccgttg cagcccagac
     2161 actcctgagc tcagacacca aggctccggg gagcagctcc tgtggggcag aacggctaca
     2221 cacagttggg ggacctggct cagcccggcc ccgagctttc tcccacagcg gggtacacag
     2281 cctggacggc ggagaagtag acagtcaggc gctacaggaa ctgacgcaga tggtgtctgg
     2341 ccctgcatcg tactctggcc caaagccttc tacccagtat ggagctccag gaccctttgc
     2401 agcccctggt gagggaggtg ccttggcggc cactgggcgg cccccgctgc tgcccacccg
     2461 agcttctcgt tctcagcgtg cggccagtga ggacatgacg agtgatgagg agcgcatggt
     2521 catctgtgag gaggaagggg atgatgatgt cattgctgac gatggcttcg gcaccactga
     2581 cattgatctc aagtgcaagg agcgggtgac cgacagcgag agtggggaca gctctgggga
     2641 ggacccagag ggcaacaagg gctttggtcg gaaggtgttt tcacctgtga tccgttcctc
     2701 ctttacccac tgccgccccc cactggaccc tgagccccca gggcccccgg atcctcctgt
     2761 agcctttggc aaaggctatg gttccgcccc atcctcctct gcgtcctcgc ctgcttcctc
     2821 ctcagcctcg gcagccacct ccttctcact gggctcagga accttcaagg cccaggagtc
     2881 tggtcagggc agcacagcgg gccccctacg gcccccaccc cctggggctg ggggtccagc
     2941 gacaccttcc aaggcaaccc ggttcctccc aatggatcct gccaccttcc ggcgcaagag
     3001 acccgaaagt gtgggtggcc tggagccacc aggcccctca gtcatcgcgg cccctcccag
     3061 cggaggagga aacatcctgc agacactggt gctgccccca aacaaggagg agcaagaggg
     3121 cggcggagcc agagtgccct ccgcccccgc cccatcactg gcctatgggg ccccagcagc
     3181 tcccctgtcc cgtcctgccg ccaccatggt caccaatgtg gtgcggcctg tcagcagcac
     3241 tcctgtgccc atcgcctcta agcccttccc cacctctggc cgggctgagg cgtctccaaa
     3301 tgacacagca ggtgccagga ctgaaatggg cactgggtct cgggtgcctg ggggctcccc
     3361 gctgggtgtc agcttagtgt attcggacaa gaagtcggca gcagccacct caccagcccc
     3421 acacttggtg gctggacccc tgctgggcac tgtggggaag gcgcctgcca ctgtcactaa
     3481 cctactggtg ggcaccccgg ggtatggggc ccctgcgccc cctgctgtcc agttcattgc
     3541 ccagggggcc cctggtggtg ggaccactgc gggctcagga gcaggtgctg ggagtggccc
     3601 caatgggcca gtacccctgg gcatcctgca accaggtgcc ctgggcaagg ctgggggaat
     3661 cacccaggta cagtacatcc tgcccacgct gccccagcag cttcaggtgg cacctgcccc
     3721 agcaccagcc cctgggacca aggcagcggc tcccagcggc cctgcaccca ccaccagcat
     3781 ccgtttcacc ctcccaccgg gcacttccac caacggcaaa gtcctggctg ccactgcacc
     3841 cactcctggc atccccatcc tgcagtctgt accctccgcc ccacccccca aagcccagtc
     3901 agtttctccc gtgcaggccc cgcccccggg tggctcagcc cagctgctgc ctgggaaggt
     3961 cctagtgcct ctggccgccc ctagcatgtc agtgcggggt ggaggggccg gccagccact
     4021 gccactggtg agcccgccct tctcagtacc tgtgcagaat ggtgcccagc cccccagcaa
     4081 gatcatccag ctgaccccgg tgcctgtgag cacacccagc ggcctggtgc cgcccctgag
     4141 cccagccaca ctccctggac ccacctctca gcctcagaag gtcctgttgc cctcctccac
     4201 cagaatcacc tatgtgcagt cagcgggcgg gcacgcgctg cccctgggta ccagccctgc
     4261 gtccagccag gctggaacag tcacctcgta cgggcccacg agctctgtag ctctaggctt
     4321 cacctcgctg gggcccagcg gccccgcctt cgtgcagccc ctgctctcag caggccaagc
     4381 cccactgctg gctcccggtc aggtgggcgt gtcacctgtg cccagtcccc agctgccgcc
     4441 tgcctgtgca gcccccggag gtcctgtcat aacagcattt tactctggca gccctgcacc
     4501 cacctcctca gcacccctgg cccagccatc ccaggccccc ccaagcctgg tctacactgt
     4561 ggccaccagc acaaccccac ctgcagccac cattctgccc aagggcccgc cagcccctgc
     4621 cactgccacc ccagccccga ctagcccttt ccccagcgcc acagcaggtt ccatgaccta
     4681 cagcttagtg gcccccaagg cccagcggcc cagcccgaag gccccccaga aagtgaaggc
     4741 agccatcgcc agcattcccg tggggtcctt tgaggcaggt gcctctgggc ggcctggccc
     4801 tgcaccccgg cagcctctgg agcctggccc agtccgagag ccaactgccc cagagtctga
     4861 gcttgagggg cagcccacac caccagcccc tccacccctg ccagagacct ggactcccac
     4921 ggcccggagc agccccccac tgcccccacc tgctgaggag cggaccagcg ccaagggccc
     4981 tgagaccatg gccagcaaat tccccagctc atcttcagac tggcgcgtcc ctgggcaggg
     5041 cctggagaat cgtggggagc ctcccactcc tcccagcccg gccccagctc cagctgtagc
     5101 ccctggtggc agcagcgaga gcagcagtgg gcgggcagcc ggggacaccc cggagcgcaa
     5161 ggaggcggct ggtactggca agaaggtgaa ggtgcggccc ccgcccctga agaagacctt
     5221 tgactctgtg gacaacaggg tcctgtcaga agtggacttc gaagagcgct ttgctgagtt
     5281 gcctgagttt cggcctgagg aggtgctgcc ctcccccacc ctgcagtctc tggccacctc
     5341 accccgggcc atcctgggct cttaccgcaa gaagaggaag aactccacgg acctggattc
     5401 agcacccgag gaccccacct cgcccaagcg caagatgaga agacgctcca gctgcagctc
     5461 ggagcccaac acccccaaga gtgccaagtg cgagggggac atcttcacct ttgaccgtac
     5521 aggtacagaa gccgaggacg tgcttgggga gctagagtat gacaaggtgc catactcctc
     5581 cctgcggcgc accctggacc agcgccgggc cctggtcatg cagctctttc aggaccatgg
     5641 cttcttcccg tcagcccagg ccacagccgc cttccaggcc cgctatgcag acatctttcc
     5701 ctccaaggtt tgtctgcagt tgaagatccg tgaggtgcgc cagaagatca tgcaggctgc
     5761 cactcccacg gagcagcccc ctggagctga ggctcctctc cctgtaccgc cccccactgg
     5821 caccgctgct gcccctgccc ccactcccag ccccgcaggg ggccctgacc ccacctcacc
     5881 cagctcggac tctggcacgg cccaggctgc cccgccactg cctccacccc cagagtcggg
     5941 gcctggacag cctggctggg agggggctcc ccagccctcc cccccacccc caggtccctc
     6001 cacagctgcc acaggcaggt agtaatgaac tagtccgtaa cttgaaagta tttcgatttc
     6061 ttggctttat atatcttgtg gaaaggacga caaccgccaa aggaccgctg cgacacgcgt
     6121 taagtcgaca atcaacctct ggattacaaa atttgtgaaa gattgactgg tattcttaac
     6181 tatgttgctc cttttacgct atgtggatac gctgctttaa tgcctttgta tcatgctatt
     6241 gcttcccgta tggctttcat tttctcctcc ttgtataaat cctggttgct gtctctttat
     6301 gaggagttgt ggcccgttgt caggcaacgt ggcgtggtgt gcactgtgtt tgctgacgca
     6361 acccccactg gttggggcat tgccaccacc tgtcagctcc tttccgggac tttcgctttc
     6421 cccctcccta ttgccacggc ggaactcatc gccgcctgcc ttgcccgctg ctggacaggg
     6481 gctcggctgt tgggcactga caattccgtg gtgttgtcgg ggaaatcatc gtcctttcct
     6541 tggctgctcg cctgtgttgc cacctggatt ctgcgcggga cgtccttctg ctacgtccct
     6601 tcggccctca atccagcgga ccttccttcc cgcggcctgc tgccggctct gcggcctctt
     6661 ccgcgtcttc gccttcgccc tcagacgagt cggatctccc tttgggccgc ctccccgcgt
     6721 cgactttaag accaatgact tacaaggcag ctgtagatct tagccacttt ttaaaagaaa
     6781 aggggggact ggaagggcta attcactccc aacgaagaca agatctgctt tttgcttgta
     6841 ctgggtctct ctggttagac cagatctgag cctgggagct ctctggctaa ctagggaacc
     6901 cactgcttaa gcctcaataa agcttgcctt gagtgcttca agtagtgtgt gcccgtctgt
     6961 tgtgtgactc tggtaactag agatccctca gaccctttta gtcagtgtgg aaaatctcta
     7021 gcagtacgta tagtagttca tgtcatctta ttattcagta tttataactt gcaaagaaat
     7081 gaatatcaga gagtgagagg aacttgttta ttgcagctta taatggttac aaataaagca
     7141 atagcatcac aaatttcaca aataaagcat ttttttcact gcattctagt tgtggtttgt
     7201 ccaaactcat caatgtatct tatcatgtct ggctctagct atcccgcccc taactccgcc
     7261 catcccgccc ctaactccgc ccagttccgc ccattctccg ccccatggct gactaatttt
     7321 ttttatttat gcagaggccg aggccgcctc ggcctctgag ctattccaga agtagtgagg
     7381 aggctttttt ggaggcctag ggacgtaccc aattcgccct atagtgagtc gtattacgcg
     7441 cgctcactgg ccgtcgtttt acaacgtcgt gactgggaaa accctggcgt tacccaactt
     7501 aatcgccttg cagcacatcc ccctttcgcc agctggcgta atagcgaaga ggcccgcacc
     7561 gatcgccctt cccaacagtt gcgcagcctg aatggcgaat gggacgcgcc ctgtagcggc
     7621 gcattaagcg cggcgggtgt ggtggttacg cgcagcgtga ccgctacact tgccagcgcc
     7681 ctagcgcccg ctcctttcgc tttcttccct tcctttctcg ccacgttcgc cggctttccc
     7741 cgtcaagctc taaatcgggg gctcccttta gggttccgat ttagtgcttt acggcacctc
     7801 gaccccaaaa aacttgatta gggtgatggt tcacgtagtg ggccatcgcc ctgatagacg
     7861 gtttttcgcc ctttgacgtt ggagtccacg ttctttaata gtggactctt gttccaaact
     7921 ggaacaacac tcaaccctat ctcggtctat tcttttgatt tataagggat tttgccgatt
     7981 tcggcctatt ggttaaaaaa tgagctgatt taacaaaaat ttaacgcgaa ttttaacaaa
     8041 atattaacgc ttacaattta ggtggcactt ttcggggaaa tgtgcgcgga acccctattt
     8101 gtttattttt ctaaatacat tcaaatatgt atccgctcat gagacaataa ccctgataaa
     8161 tgcttcaata atattgaaaa aggaagagta tgagtattca acatttccgt gtcgccctta
     8221 ttcccttttt tgcggcattt tgccttcctg tttttgctca cccagaaacg ctggtgaaag
     8281 taaaagatgc tgaagatcag ttgggtgcac gagtgggtta catcgaactg gatctcaaca
     8341 gcggtaagat ccttgagagt tttcgccccg aagaacgttt tccaatgatg agcactttta
     8401 aagttctgct atgtggcgcg gtattatccc gtattgacgc cgggcaagag caactcggtc
     8461 gccgcataca ctattctcag aatgacttgg ttgagtactc accagtcaca gaaaagcatc
     8521 ttacggatgg catgacagta agagaattat gcagtgctgc cataaccatg agtgataaca
     8581 ctgcggccaa cttacttctg acaacgatcg gaggaccgaa ggagctaacc gcttttttgc
     8641 acaacatggg ggatcatgta actcgccttg atcgttggga accggagctg aatgaagcca
     8701 taccaaacga cgagcgtgac accacgatgc ctgtagcaat ggcaacaacg ttgcgcaaac
     8761 tattaactgg cgaactactt actctagctt cccggcaaca attaatagac tggatggagg
     8821 cggataaagt tgcaggacca cttctgcgct cggcccttcc ggctggctgg tttattgctg
     8881 ataaatctgg agccggtgag cgtgggtctc gcggtatcat tgcagcactg gggccagatg
     8941 gtaagccctc ccgtatcgta gttatctaca cgacggggag tcaggcaact atggatgaac
     9001 gaaatagaca gatcgctgag ataggtgcct cactgattaa gcattggtaa ctgtcagacc
     9061 aagtttactc atatatactt tagattgatt taaaacttca tttttaattt aaaaggatct
     9121 aggtgaagat cctttttgat aatctcatga ccaaaatccc ttaacgtgag ttttcgttcc
     9181 actgagcgtc agaccccgta gaaaagatca aaggatcttc ttgagatcct ttttttctgc
     9241 gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc agcggtggtt tgtttgccgg
     9301 atcaagagct accaactctt tttccgaagg taactggctt cagcagagcg cagataccaa
     9361 atactgttct tctagtgtag ccgtagttag gccaccactt caagaactct gtagcaccgc
     9421 ctacatacct cgctctgcta atcctgttac cagtggctgc tgccagtggc gataagtcgt
     9481 gtcttaccgg gttggactca agacgatagt taccggataa ggcgcagcgg tcgggctgaa
     9541 cggggggttc gtgcacacag cccagcttgg agcgaacgac ctacaccgaa ctgagatacc
     9601 tacagcgtga gctatgagaa agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc
     9661 cggtaagcgg cagggtcgga acaggagagc gcacgaggga gcttccaggg ggaaacgcct
     9721 ggtatcttta tagtcctgtc gggtttcgcc acctctgact tgagcgtcga tttttgtgat
     9781 gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa cgcggccttt ttacggttcc
     9841 tggccttttg ctggcctttt gctcacatgt tctttcctgc gttatcccct gattctgtgg
     9901 ataaccgtat taccgccttt gagtgagctg ataccgctcg ccgcagccga acgaccgagc
     9961 gcagcgagtc agtgagcgag gaagcggaag agcgcccaat acgcaaaccg cctctccccg
    10021 cgcgttggcc gattcattaa tgcagctggc acgacaggtt tcccgactgg aaagcgggca
    10081 gtgagcgcaa cgcaattaat gtgagttagc tcactcatta ggcaccccag gctttacact
    10141 ttatgcttcc ggctcgtatg ttgtgtggaa ttgtgagcgg ataacaattt cacacaggaa
    10201 acagctatga ccatgattac gccaagcgcg caattaaccc tcactaaagg gaacaaaagc
    10261 tggagctgca agcttaatgt agtcttatgc aatactcttg tagtcttgca acatggtaac
    10321 gatgagttag caacatgcct tacaaggaga gaaaaagcac cgtgcatgcc gattggtgga
    10381 agtaaggtgg tacgatcgtg ccttattagg aaggcaacag acgggtctga catggattgg
    10441 acgaaccact gaattgccgc attgcagaga tattgtattt aagtgcctag ctcgatacat
    10501 aaacgggtct ctctggttag accagatctg agcctgggag ctctctggct aactagggaa
    10561 cccactgctt aagcctcaat aaagcttgcc ttgagtgctt caagtagtgt gtgcccgtct
    10621 gttgtgtgac tctggtaact agagatccct cagacccttt tagtcagtgt ggaaaatctc
    10681 tagcagtggc gcccgaacag ggacttgaaa gcgaaaggga aaccagagga gctctctcga
    10741 cgcaggactc ggcttgctga agcgcgcacg gcaagaggcg aggggcggcg actggtgagt
    10801 acgccaaaaa ttttgactag cggaggctag aaggagagag atgggtgcga gagcgtcagt
    10861 attaagcggg ggagaattag atcgcgatgg gaaaaaattc ggttaaggcc agggggaaag
    10921 aaaaaatata aattaaaaca tatagtatgg gcaagcaggg agctagaacg attcgcagtt
    10981 aatcctggcc tgttagaaac atcagaaggc tgtagacaaa tactgggaca gctacaacca
    11041 tcccttcaga caggatcaga agaacttaga tcattatata atacagtagc aaccctctat
    11101 tgtgtgcatc aaaggataga gataaaagac accaaggaag ctttagacaa gatagaggaa
    11161 gagcaaaaca aaagtaagac caccgcacag caagcggccg ctgatcttca gacctggagg
    11221 aggagatatg agggacaatt ggagaagtga attatataaa tataaagtag taaaaattga
    11281 accattagga gtagcaccca ccaaggcaaa gagaagagtg gtgcagagag aaaaaagagc
    11341 agtgggaata ggagctttgt tccttgggtt cttgggagca gcaggaagca ctatgggcgc
    11401 agcgtcaatg acgctgacgg tacaggccag acaattattg tctggtatag tgcagcagca
    11461 gaacaatttg ctgagggcta ttgaggcgca acagcatctg ttgcaactca cagtctgggg
    11521 catcaagcag ctccaggcaa gaatcctggc tgtggaaaga tacctaaagg atcaacagct
    11581 cctggggatt tggggttgct ctggaaaact catttgcacc actgctgtgc cttggaatgc
    11641 tagttggagt aataaatctc tggaacagat ttggaatcac acgacctgga tggagtggga
    11701 cagagaaatt aacaattaca caagcttaat acactcctta attgaagaat cgcaaaacca
    11761 gcaagaaaag aatgaacaag aattattgga attagataaa tgggcaagtt tgtggaattg
    11821 gtttaacata acaaattggc tgtggtatat aaaattattc ataatgatag taggaggctt
    11881 ggtaggttta agaatagttt ttgctgtact ttctatagtg aatagagtta ggcagggata
    11941 ttcaccatta tcgtttcaga cccacctccc aaccccgagg ggacccatgc attgcatctc
    12001 aattagtcag caaccaggtg tggaaagtcc ccaggctccc cagcaggcag aagtatgcaa
    12061 agcatgcgtc tcaattagtc agcaaccata gtcccgcccc taactccgcc catcccgccc
    12121 ctaactccgc ccagttccgc ccattctccg ccccatggct gactaatttt ttttatttat
    12181 gcagaggccg aggccgcctc ggcctctgag ctattccaga agtagtgagg aggctttttt
    12241 ggaggcctag gcttttgcaa aaagctttct agaggtacca ccggagctta ccatgaccga
    12301 gtacaagccc acggtgcgcc tcgccacccg cgacgacgtc cccagggccg tacgcaccct
    12361 cgccgccgcg ttcgccgact accccgccac gcgccacacc gtcgatccgg accgccacat
    12421 cgagcgggtc accgagctgc aagaactctt cctcacgcgc gtcgggctcg acatcggcaa
    12481 ggtgtgggtc gcggacgacg gcgccgccgt ggcggtctgg accacgccgg agagcgtcga
    12541 agcgggggcg gtgttcgccg agatcggccc gcgcatggcc gagttgagcg gttcccggct
    12601 ggccgcgcag caacagatgg aaggcctcct ggcgccgcac cggcccaagg agcccgcgtg
    12661 gttcctggcc accgtcggcg tctcgcccga ccaccagggc aagggtctgg gcagcgccgt
    12721 cgtgctcccc ggagtggagg cggccgagcg cgccggggtg cccgccttcc tggagacctc
    12781 cgcgccccgc aacctcccct tctacgagcg gctcggcttc accgtcaccg ccgacgtcga
    12841 ggtgcccgaa ggaccgcgca cctggtgcat gacccgcaag cccggtgcca gttaacgaat
    12901 tctagatctt gagacaaatg gcagtattca tccacaattt taaaagaaaa ggggggattg
    12961 gggggtacag tgcaggggaa agaatagtag acataatagc aacagacata caaactaaag
    13021 aattacaaaa acaaattaca aaaattcaaa attttcgggt ttattacagg gacagcagag
    13081 atccactttg gcgccggctc gaggcctgca ggtgcaaaga tggataaagt tttaaacaga
    13141 gaggaatctt tgcagctaat ggaccttcta ggtcttgaaa ggagtgggaa tt
//
