LOCUS       sequence-35333-e         788 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 788)
  AUTHORS   Hussein R, Lim HN
  TITLE     Disruption of small RNA signaling caused by competition for Hfq.
  JOURNAL   Proc Natl Acad Sci U S A. 2011 Jan 18;108(3):1110-5. doi: 
            10.1073/pnas.1010082108. Epub 2010 Dec 28.
  PUBMED    21189298
REFERENCE   2  (bases 1 to 788)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1073/pnas.1010082108"; journalName: "Proc Natl Acad Sci U S A"; 
            date: "2011-01-18- 18"; volume: "108"; issue: "3"; pages: "1110-5"
COMMENT     Sequence Label: HL 1178 (=HL 862 + pHL 282)
FEATURES             Location/Qualifiers
     source          1..788
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    143..161
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    168..186
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     terminator      complement(253..280)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(372..458)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     611..628
                     /label=pBAD Reverse
                     /note="For vectors with E. coli araBAD promoter, reverse 
                     primer"
     primer_bind     complement(714..734)
                     /label=GFP-F
                     /note="GFP, forward primer. Does NOT anneal to EGFP"
ORIGIN
        1 ctcgccctcg ccctcgatct cgaactcgtg gccgttcacg gagccctcca tgtgcacctt
       61 gaagcgcatg aactccttga tgatggccat gttatcctcc tcgcccttgc tcaccatttt
      121 ttttcctcct tacccgggag tatctctatc actgataggg atgtcaatct ctatcactga
      181 tagggaggat cctgagcgga tacatatttg aatgtattta gaaaaataaa caaaagagtt
      241 tgtagaaacg caaaaaggcc atccgtcagg atggccttct gcttaatttg atgcctggca
      301 gtttatggcg ggcgtcctgc ccgccaccct ccgggccgtt gcttcgcaac gttcaaatcc
      361 gctcccggcg gatttgtcct actcaggaga gcgttcaccg acaaacaaca gataaaacga
      421 aaggcccagt ctttcgactg agcctttcgt tttatttgat gcctggcagt tccctactct
      481 cgcatgggga gaccccacac taccatcggc gctacggcgt ttcacttctg agttcggcat
      541 ggggtcaggt gggaccaccg cgctactgcc gccaggcaaa ttctgtttta tcagaccgct
      601 tctgcgttct gatttaatct gtatcaggct gaaaatcttc tctcatccgc caaaacagcc
      661 aagctagctt ttatttgtag agctcatcca tgccatgtgt aatcccagca gctgttacaa
      721 actcaagaag gaccatgtgg tctctctttt cgttgggatc tttcgaaagg gcagattgtg
      781 tggacagg
//
