LOCUS       sequence-353335-        9790 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9790)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 9790)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF3409
FEATURES             Location/Qualifiers
     source          1..9790
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        1..1179
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          232..1170
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     1127..1147
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     protein_bind    1199..1223
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(2544..2568)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             2576..2617
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     misc_feature    2728..3316
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(2781..2801)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(3199..3210)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             3388..3621
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    3699..3820
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(3736..3755)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     3790..3809
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      3860..3995
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(4015..4034)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(4016..4034)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(4044..4061)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(4044..4060)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(4053..4075)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      4202..4657
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(4289..4308)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     4499..4520
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        4683..4787
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(4788..4856,4857..5648)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(5006..5025)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      5819..6407
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     6308..6327
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     6561..6578
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    6695..6716
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(6731..6736,6737..6754,6755..6761)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6769..6785
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     6774..6796
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     6793..6809
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     6793..6809
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     6828..6848
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        6830..6848
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        6876..7102
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             7103..7283
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    7330..7455
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    7948..8181
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             8366..8410
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     promoter        8641..8851
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     promoter        8663..8859
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     rep_origin      8710..8845
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             8891..9493
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGAS"
     primer_bind     complement(8891..8910)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     9387..9407
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     misc_feature    9536..9653
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
ORIGIN
        1 ggctccggtg cccgtcagtg ggcagagcgc acatcgccca cagtccccga gaagttgggg
       61 ggaggggtcg gcaattgaac cggtgcctag agaaggtggc gcggggtaaa ctgggaaagt
      121 gatgtcgtgt actggctccg cctttttccc gagggtgggg gagaaccgta tataagtgca
      181 gtagtcgccg tgaacgttct ttttcgcaac gggtttgccg ccagaacaca ggtaagtgcc
      241 gtgtgtggtt cccgcgggcc tggcctcttt acgggttatg gcccttgcgt gccttgaatt
      301 acttccacct ggctgcagta cgtgattctt gatcccgagc ttcgggttgg aagtgggtgg
      361 gagagttcga ggccttgcgc ttaaggagcc ccttcgcctc gtgcttgagt tgaggcctgg
      421 cctgggcgct ggggccgccg cgtgcgaatc tggtggcacc ttcgcgcctg tctcgctgct
      481 ttcgataagt ctctagccat ttaaaatttt tgatgacctg ctgcgacgct ttttttctgg
      541 caagatagtc ttgtaaatgc gggccaagat ctgcacactg gtatttcggt ttttggggcc
      601 gcgggcggcg acggggcccg tgcgtcccag cgcacatgtt cggcgaggcg gggcctgcga
      661 gcgcggccac cgagaatcgg acgggggtag tctcaagctg gccggcctgc tctggtgcct
      721 ggcctcgcgc cgccgtgtat cgccccgccc tgggcggcaa ggctggcccg gtcggcacca
      781 gttgcgtgag cggaaagatg gccgcttccc ggccctgctg cagggagctc aaaatggagg
      841 acgcggcgct cgggagagcg ggcgggtgag tcacccacac aaaggaaaag ggcctttccg
      901 tcctcagccg tcgcttcatg tgactccacg gagtaccggg cgccgtccag gcacctcgat
      961 tagttctcga gcttttggag tacgtcgtct ttaggttggg gggaggggtt ttatgcgatg
     1021 gagtttcccc acactgagtg ggtggagact gaagttaggc cagcttggca cttgatgtaa
     1081 ttctccttgg aatttgccct ttttgagttt ggatcttggt tcattctcaa gcctcagaca
     1141 gtggttcaaa gtttttttct tccatttcag gtgtcgtgag gctagcatcg attgatcaac
     1201 aagtttgtac aaaaaagttg gcaccatgct ccaaacaact tggcctcagg agtcagtgac
     1261 ctttgaggat gtggctgttt acttcaccca gaatcaatgg gccagcctcg accctgcgca
     1321 gagggccctg tacggggagg tgatgctgga gaattatgca aatgtggctt ctctggtagc
     1381 gtttccattc cccaaacctg ctctgatctc ccacctggag agaggggaag caccatgggg
     1441 cccagatccc tgggacaccg agattctgag aggcatcagt caaggtggtg agtcctggat
     1501 caaaaatgaa gggctagtta taaagcagga agcctctgaa gaaacagagt tgcacagaat
     1561 gccagtagga ggacttctca ggaacgtttc tcagcacttt gattttaaaa ggaaggcact
     1621 gaagcagact ttcaatctaa atccaaatct gatacttcga ggtggaatga agttctatga
     1681 atgtaaagaa tgtgggaaaa tcttccgata taactcaaag cttattcggc atcagatgag
     1741 tcatactggg gaaaagccct ttaagtgtaa ggagtgtggc aaagctttca agtccagcta
     1801 tgattgtatt gtacatgaga aaaaccacat tggagaaggg ccctatgaat gtaaggagtg
     1861 tggcaaaggt ttgagttcca acacagcctt gactcaacat cagaggatcc acactggaga
     1921 gaaaccctat gaatgtaaag agtgtggaaa ggctttccgt aggagtgcgg catacctgca
     1981 gcatcagaga ttacacacgg gagagaaact ctataaatgt aaggaatgtt ggaaagcttt
     2041 cggttgtagg tcacttttta ttgtccatca gagaattcat actggggaga aaccttatca
     2101 atgtaaggag tgtggcaaag ccttcaccca gaaaatagcc tccattcagc atcagagagt
     2161 tcacactggg gagaagcctt atgaatgtaa ggtgtgtggg aaagccttca aatggtatgg
     2221 aagttttgtt cagcatcaga aattgcaccc tgtggagaag aagccagtca aggtccttgg
     2281 gccatccctg gtcagtcccc agtgctcctc tccagccata cctcctgttc ttctccaggg
     2341 atcctgttct gcttcagccg tagctgtgcc ttcactgacc tttccacatg ctgtgctcat
     2401 tcctacctct gggaattttt tcatgctgct gcctacatct ggaatacctt cttcatctgc
     2461 ccaaatagtg cgtgtcttcc agggtcttac tcccactgtg aaaccttccc cagttattct
     2521 caccccttct tctcactcct cattgccaac tttcttgtac aaagtggttg atatcggtaa
     2581 gcctatccct aaccctctcc tcggtctcga ttctacgtag taatgaacta gtccgtaact
     2641 tgaaagtatt tcgatttctt ggctttatat atcttgtgga aaggacgaat agacgtttac
     2701 attagttcgc gcacgcgtta agtcgacaat caacctctgg attacaaaat ttgtgaaaga
     2761 ttgactggta ttcttaacta tgttgctcct tttacgctat gtggatacgc tgctttaatg
     2821 cctttgtatc atgctattgc ttcccgtatg gctttcattt tctcctcctt gtataaatcc
     2881 tggttgctgt ctctttatga ggagttgtgg cccgttgtca ggcaacgtgg cgtggtgtgc
     2941 actgtgtttg ctgacgcaac ccccactggt tggggcattg ccaccacctg tcagctcctt
     3001 tccgggactt tcgctttccc cctccctatt gccacggcgg aactcatcgc cgcctgcctt
     3061 gcccgctgct ggacaggggc tcggctgttg ggcactgaca attccgtggt gttgtcgggg
     3121 aaatcatcgt cctttccttg gctgctcgcc tgtgttgcca cctggattct gcgcgggacg
     3181 tccttctgct acgtcccttc ggccctcaat ccagcggacc ttccttcccg cggcctgctg
     3241 ccggctctgc ggcctcttcc gcgtcttcgc cttcgccctc agacgagtcg gatctccctt
     3301 tgggccgcct ccccgcgtcg actttaagac caatgactta caaggcagct gtagatctta
     3361 gccacttttt aaaagaaaag gggggactgg aagggctaat tcactcccaa cgaagacaag
     3421 atctgctttt tgcttgtact gggtctctct ggttagacca gatctgagcc tgggagctct
     3481 ctggctaact agggaaccca ctgcttaagc ctcaataaag cttgccttga gtgcttcaag
     3541 tagtgtgtgc ccgtctgttg tgtgactctg gtaactagag atccctcaga cccttttagt
     3601 cagtgtggaa aatctctagc agtacgtata gtagttcatg tcatcttatt attcagtatt
     3661 tataacttgc aaagaaatga atatcagaga gtgagaggaa cttgtttatt gcagcttata
     3721 atggttacaa ataaagcaat agcatcacaa atttcacaaa taaagcattt ttttcactgc
     3781 attctagttg tggtttgtcc aaactcatca atgtatctta tcatgtctgg ctctagctat
     3841 cccgccccta actccgccca tcccgcccct aactccgccc agttccgccc attctccgcc
     3901 ccatggctga ctaatttttt ttatttatgc agaggccgag gccgcctcgg cctctgagct
     3961 attccagaag tagtgaggag gcttttttgg aggcctaggg acgtacccaa ttcgccctat
     4021 agtgagtcgt attacgcgcg ctcactggcc gtcgttttac aacgtcgtga ctgggaaaac
     4081 cctggcgtta cccaacttaa tcgccttgca gcacatcccc ctttcgccag ctggcgtaat
     4141 agcgaagagg cccgcaccga tcgcccttcc caacagttgc gcagcctgaa tggcgaatgg
     4201 gacgcgccct gtagcggcgc attaagcgcg gcgggtgtgg tggttacgcg cagcgtgacc
     4261 gctacacttg ccagcgccct agcgcccgct cctttcgctt tcttcccttc ctttctcgcc
     4321 acgttcgccg gctttccccg tcaagctcta aatcgggggc tccctttagg gttccgattt
     4381 agtgctttac ggcacctcga ccccaaaaaa cttgattagg gtgatggttc acgtagtggg
     4441 ccatcgccct gatagacggt ttttcgccct ttgacgttgg agtccacgtt ctttaatagt
     4501 ggactcttgt tccaaactgg aacaacactc aaccctatct cggtctattc ttttgattta
     4561 taagggattt tgccgatttc ggcctattgg ttaaaaaatg agctgattta acaaaaattt
     4621 aacgcgaatt ttaacaaaat attaacgctt acaatttagg tggcactttt cggggaaatg
     4681 tgcgcggaac ccctatttgt ttatttttct aaatacattc aaatatgtat ccgctcatga
     4741 gacaataacc ctgataaatg cttcaataat attgaaaaag gaagagtatg agtattcaac
     4801 atttccgtgt cgcccttatt cccttttttg cggcattttg ccttcctgtt tttgctcacc
     4861 cagaaacgct ggtgaaagta aaagatgctg aagatcagtt gggtgcacga gtgggttaca
     4921 tcgaactgga tctcaacagc ggtaagatcc ttgagagttt tcgccccgaa gaacgttttc
     4981 caatgatgag cacttttaaa gttctgctat gtggcgcggt attatcccgt attgacgccg
     5041 ggcaagagca actcggtcgc cgcatacact attctcagaa tgacttggtt gagtactcac
     5101 cagtcacaga aaagcatctt acggatggca tgacagtaag agaattatgc agtgctgcca
     5161 taaccatgag tgataacact gcggccaact tacttctgac aacgatcgga ggaccgaagg
     5221 agctaaccgc ttttttgcac aacatggggg atcatgtaac tcgccttgat cgttgggaac
     5281 cggagctgaa tgaagccata ccaaacgacg agcgtgacac cacgatgcct gtagcaatgg
     5341 caacaacgtt gcgcaaacta ttaactggcg aactacttac tctagcttcc cggcaacaat
     5401 taatagactg gatggaggcg gataaagttg caggaccact tctgcgctcg gcccttccgg
     5461 ctggctggtt tattgctgat aaatctggag ccggtgagcg tgggtctcgc ggtatcattg
     5521 cagcactggg gccagatggt aagccctccc gtatcgtagt tatctacacg acggggagtc
     5581 aggcaactat ggatgaacga aatagacaga tcgctgagat aggtgcctca ctgattaagc
     5641 attggtaact gtcagaccaa gtttactcat atatacttta gattgattta aaacttcatt
     5701 tttaatttaa aaggatctag gtgaagatcc tttttgataa tctcatgacc aaaatccctt
     5761 aacgtgagtt ttcgttccac tgagcgtcag accccgtaga aaagatcaaa ggatcttctt
     5821 gagatccttt ttttctgcgc gtaatctgct gcttgcaaac aaaaaaacca ccgctaccag
     5881 cggtggtttg tttgccggat caagagctac caactctttt tccgaaggta actggcttca
     5941 gcagagcgca gataccaaat actgttcttc tagtgtagcc gtagttaggc caccacttca
     6001 agaactctgt agcaccgcct acatacctcg ctctgctaat cctgttacca gtggctgctg
     6061 ccagtggcga taagtcgtgt cttaccgggt tggactcaag acgatagtta ccggataagg
     6121 cgcagcggtc gggctgaacg gggggttcgt gcacacagcc cagcttggag cgaacgacct
     6181 acaccgaact gagataccta cagcgtgagc tatgagaaag cgccacgctt cccgaaggga
     6241 gaaaggcgga caggtatccg gtaagcggca gggtcggaac aggagagcgc acgagggagc
     6301 ttccaggggg aaacgcctgg tatctttata gtcctgtcgg gtttcgccac ctctgacttg
     6361 agcgtcgatt tttgtgatgc tcgtcagggg ggcggagcct atggaaaaac gccagcaacg
     6421 cggccttttt acggttcctg gccttttgct ggccttttgc tcacatgttc tttcctgcgt
     6481 tatcccctga ttctgtggat aaccgtatta ccgcctttga gtgagctgat accgctcgcc
     6541 gcagccgaac gaccgagcgc agcgagtcag tgagcgagga agcggaagag cgcccaatac
     6601 gcaaaccgcc tctccccgcg cgttggccga ttcattaatg cagctggcac gacaggtttc
     6661 ccgactggaa agcgggcagt gagcgcaacg caattaatgt gagttagctc actcattagg
     6721 caccccaggc tttacacttt atgcttccgg ctcgtatgtt gtgtggaatt gtgagcggat
     6781 aacaatttca cacaggaaac agctatgacc atgattacgc caagcgcgca attaaccctc
     6841 actaaaggga acaaaagctg gagctgcaag cttaatgtag tcttatgcaa tactcttgta
     6901 gtcttgcaac atggtaacga tgagttagca acatgcctta caaggagaga aaaagcaccg
     6961 tgcatgccga ttggtggaag taaggtggta cgatcgtgcc ttattaggaa ggcaacagac
     7021 gggtctgaca tggattggac gaaccactga attgccgcat tgcagagata ttgtatttaa
     7081 gtgcctagct cgatacataa acgggtctct ctggttagac cagatctgag cctgggagct
     7141 ctctggctaa ctagggaacc cactgcttaa gcctcaataa agcttgcctt gagtgcttca
     7201 agtagtgtgt gcccgtctgt tgtgtgactc tggtaactag agatccctca gaccctttta
     7261 gtcagtgtgg aaaatctcta gcagtggcgc ccgaacaggg acttgaaagc gaaagggaaa
     7321 ccagaggagc tctctcgacg caggactcgg cttgctgaag cgcgcacggc aagaggcgag
     7381 gggcggcgac tggtgagtac gccaaaaatt ttgactagcg gaggctagaa ggagagagat
     7441 gggtgcgaga gcgtcagtat taagcggggg agaattagat cgcgatggga aaaaattcgg
     7501 ttaaggccag ggggaaagaa aaaatataaa ttaaaacata tagtatgggc aagcagggag
     7561 ctagaacgat tcgcagttaa tcctggcctg ttagaaacat cagaaggctg tagacaaata
     7621 ctgggacagc tacaaccatc ccttcagaca ggatcagaag aacttagatc attatataat
     7681 acagtagcaa ccctctattg tgtgcatcaa aggatagaga taaaagacac caaggaagct
     7741 ttagacaaga tagaggaaga gcaaaacaaa agtaagacca ccgcacagca agcggccgct
     7801 gatcttcaga cctggaggag gagatatgag ggacaattgg agaagtgaat tatataaata
     7861 taaagtagta aaaattgaac cattaggagt agcacccacc aaggcaaaga gaagagtggt
     7921 gcagagagaa aaaagagcag tgggaatagg agctttgttc cttgggttct tgggagcagc
     7981 aggaagcact atgggcgcag cgtcaatgac gctgacggta caggccagac aattattgtc
     8041 tggtatagtg cagcagcaga acaatttgct gagggctatt gaggcgcaac agcatctgtt
     8101 gcaactcaca gtctggggca tcaagcagct ccaggcaaga atcctggctg tggaaagata
     8161 cctaaaggat caacagctcc tggggatttg gggttgctct ggaaaactca tttgcaccac
     8221 tgctgtgcct tggaatgcta gttggagtaa taaatctctg gaacagattt ggaatcacac
     8281 gacctggatg gagtgggaca gagaaattaa caattacaca agcttaatac actccttaat
     8341 tgaagaatcg caaaaccagc aagaaaagaa tgaacaagaa ttattggaat tagataaatg
     8401 ggcaagtttg tggaattggt ttaacataac aaattggctg tggtatataa aattattcat
     8461 aatgatagta ggaggcttgg taggtttaag aatagttttt gctgtacttt ctatagtgaa
     8521 tagagttagg cagggatatt caccattatc gtttcagacc cacctcccaa ccccgagggg
     8581 acccatgcat tgcatctcaa ttagtcagca accaggtgtg gaaagtcccc aggctcccca
     8641 gcaggcagaa gtatgcaaag catgcgtctc aattagtcag caaccatagt cccgccccta
     8701 actccgccca tcccgcccct aactccgccc agttccgccc attctccgcc ccatggctga
     8761 ctaatttttt ttatttatgc agaggccgag gccgcctcgg cctctgagct attccagaag
     8821 tagtgaggag gcttttttgg aggcctaggc ttttgcaaaa agctttctag aggtaccacc
     8881 ggagcttacc atgaccgagt acaagcccac ggtgcgcctc gccacccgcg acgacgtccc
     8941 cagggccgta cgcaccctcg ccgccgcgtt cgccgactac cccgccacgc gccacaccgt
     9001 cgatccggac cgccacatcg agcgggtcac cgagctgcaa gaactcttcc tcacgcgcgt
     9061 cgggctcgac atcggcaagg tgtgggtcgc ggacgacggc gccgccgtgg cggtctggac
     9121 cacgccggag agcgtcgaag cgggggcggt gttcgccgag atcggcccgc gcatggccga
     9181 gttgagcggt tcccggctgg ccgcgcagca acagatggaa ggcctcctgg cgccgcaccg
     9241 gcccaaggag cccgcgtggt tcctggccac cgtcggcgtc tcgcccgacc accagggcaa
     9301 gggtctgggc agcgccgtcg tgctccccgg agtggaggcg gccgagcgcg ccggggtgcc
     9361 cgccttcctg gagacctccg cgccccgcaa cctccccttc tacgagcggc tcggcttcac
     9421 cgtcaccgcc gacgtcgagg tgcccgaagg accgcgcacc tggtgcatga cccgcaagcc
     9481 cggtgccagt taacgaattc tagatcttga gacaaatggc agtattcatc cacaatttta
     9541 aaagaaaagg ggggattggg gggtacagtg caggggaaag aatagtagac ataatagcaa
     9601 cagacataca aactaaagaa ttacaaaaac aaattacaaa aattcaaaat tttcgggttt
     9661 attacaggga cagcagagat ccactttggc gccggctcga ggcctgcagg tgcaaagatg
     9721 gataaagttt taaacagaga ggaatctttg cagctaatgg accttctagg tcttgaaagg
     9781 agtgggaatt
//
