LOCUS       sequence-35334-e         863 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 863)
  AUTHORS   Hussein R, Lim HN
  TITLE     Disruption of small RNA signaling caused by competition for Hfq.
  JOURNAL   Proc Natl Acad Sci U S A. 2011 Jan 18;108(3):1110-5. doi: 
            10.1073/pnas.1010082108. Epub 2010 Dec 28.
  PUBMED    21189298
REFERENCE   2  (bases 1 to 863)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1073/pnas.1010082108"; journalName: "Proc Natl Acad Sci U S A"; 
            date: "2011-01-18- 18"; volume: "108"; issue: "3"; pages: "1110-5"
COMMENT     Sequence Label: HL 839 (=HL 716 + pHL 108)
FEATURES             Location/Qualifiers
     source          1..863
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     RBS             117..128
                     /note="strong bacterial ribosome binding site (Elowitz and 
                     Leibler, 2000)"
     protein_bind    283..299
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     terminator      complement(392..419)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(511..597)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     750..767
                     /label=pBAD Reverse
                     /note="For vectors with E. coli araBAD promoter, reverse 
                     primer"
ORIGIN
        1 tcgccctcga tctcgaactc gtggccgttc acggagccct ccatgtgcac cttgaagcgc
       61 atgaactcct tgatgatggc catgttatcc tcctcgccct tgctcaccat cgtacgtttc
      121 tcctctttaa taagcttctg gataaggatt atccaattct aaaaaaaaag cccggacgac
      181 tgttcgggct tgtcttttta tatgttggaa aatcagtggc aatgcaatgg cccaacagaa
      241 aataaaatct gtgacaataa aggcatataa cccgggagta tcttgttatc cgctcacaat
      301 gtcaatgtta tccgctcaca tttatggatc ctgagcggat acatatttga atgtatttag
      361 aaaaataaac aaaagagttt gtagaaacgc aaaaaggcca tccgtcagga tggccttctg
      421 cttaatttga tgcctggcag tttatggcgg gcgtcctgcc cgccaccctc cgggccgttg
      481 cttcgcaacg ttcaaatccg ctcccggcgg atttgtccta ctcaggagag cgttcaccga
      541 caaacaacag ataaaacgaa aggcccagtc tttcgactga gcctttcgtt ttatttgatg
      601 cctggcagtt ccctactctc gcatggggag accccacact accatcggcg ctacggcgtt
      661 tcacttctga gttcggcatg gggtcaggtg ggaccaccgc gctactgccg ccaggcaaat
      721 tctgttttat cagaccgctt ctgcgttctg atttaatctg tatcaggctg aaaatcttct
      781 ctcatccgcc aaaacagcca agctagcttt tatttgtaga gctcatccat gccatgggta
      841 atcccagcag ctgttacaac tca
//
