LOCUS       sequence-353340-        9196 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9196)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 9196)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF3025
FEATURES             Location/Qualifiers
     source          1..9196
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        1..1179
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          232..1170
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     1127..1147
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     protein_bind    1199..1223
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             complement(1768..1779)
                     /codon_start=1
                     /product="WELQut protease recognition and cleavage site"
                     /label=WELQut site
                     /translation="WELQ"
     protein_bind    complement(1950..1974)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             1982..2023
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     misc_feature    2134..2722
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(2187..2207)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(2605..2616)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             2794..3027
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    3105..3226
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(3142..3161)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     3196..3215
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      3266..3401
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(3421..3440)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(3422..3440)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(3450..3467)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(3450..3466)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(3459..3481)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      3608..4063
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(3695..3714)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     3905..3926
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        4089..4193
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(4194..4262,4263..5054)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(4412..4431)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      5225..5813
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     5714..5733
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     5967..5984
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    6101..6122
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(6137..6142,6143..6160,6161..6167)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6175..6191
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     6180..6202
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     6199..6215
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     6199..6215
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     6234..6254
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        6236..6254
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        6282..6508
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             6509..6689
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    6736..6861
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    7354..7587
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             7772..7816
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     promoter        8047..8257
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     promoter        8069..8265
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     rep_origin      8116..8251
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             8297..8899
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGAS"
     primer_bind     complement(8297..8316)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     8793..8813
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     misc_feature    8942..9059
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
ORIGIN
        1 ggctccggtg cccgtcagtg ggcagagcgc acatcgccca cagtccccga gaagttgggg
       61 ggaggggtcg gcaattgaac cggtgcctag agaaggtggc gcggggtaaa ctgggaaagt
      121 gatgtcgtgt actggctccg cctttttccc gagggtgggg gagaaccgta tataagtgca
      181 gtagtcgccg tgaacgttct ttttcgcaac gggtttgccg ccagaacaca ggtaagtgcc
      241 gtgtgtggtt cccgcgggcc tggcctcttt acgggttatg gcccttgcgt gccttgaatt
      301 acttccacct ggctgcagta cgtgattctt gatcccgagc ttcgggttgg aagtgggtgg
      361 gagagttcga ggccttgcgc ttaaggagcc ccttcgcctc gtgcttgagt tgaggcctgg
      421 cctgggcgct ggggccgccg cgtgcgaatc tggtggcacc ttcgcgcctg tctcgctgct
      481 ttcgataagt ctctagccat ttaaaatttt tgatgacctg ctgcgacgct ttttttctgg
      541 caagatagtc ttgtaaatgc gggccaagat ctgcacactg gtatttcggt ttttggggcc
      601 gcgggcggcg acggggcccg tgcgtcccag cgcacatgtt cggcgaggcg gggcctgcga
      661 gcgcggccac cgagaatcgg acgggggtag tctcaagctg gccggcctgc tctggtgcct
      721 ggcctcgcgc cgccgtgtat cgccccgccc tgggcggcaa ggctggcccg gtcggcacca
      781 gttgcgtgag cggaaagatg gccgcttccc ggccctgctg cagggagctc aaaatggagg
      841 acgcggcgct cgggagagcg ggcgggtgag tcacccacac aaaggaaaag ggcctttccg
      901 tcctcagccg tcgcttcatg tgactccacg gagtaccggg cgccgtccag gcacctcgat
      961 tagttctcga gcttttggag tacgtcgtct ttaggttggg gggaggggtt ttatgcgatg
     1021 gagtttcccc acactgagtg ggtggagact gaagttaggc cagcttggca cttgatgtaa
     1081 ttctccttgg aatttgccct ttttgagttt ggatcttggt tcattctcaa gcctcagaca
     1141 gtggttcaaa gtttttttct tccatttcag gtgtcgtgag gctagcatcg attgatcaac
     1201 aagtttgtac aaaaaagttg gcatgatgat ggaccttttt gaaactggct cctatttctt
     1261 ctacttggat ggggaaaatg ttactctgca gccattagaa gtggcagaag gctctccttt
     1321 gtatccaggg agtgatggta ccttgtcccc ctgccaggac caaatgcccc cggaagcggg
     1381 gagcgacagc agcggagagg aacatgtcct ggcgcccccg ggcctgcagc ctccacactg
     1441 ccccggccag tgtctgatct gggcttgcaa gacctgcaag agaaaatctg cccccactga
     1501 ccggcgaaaa gccgccaccc tgcgcgaaag gaggaggcta aagaaaatca acgaggcctt
     1561 cgaggcactg aagcggcgaa ctgtggccaa ccccaaccag aggctgccca aggtggagat
     1621 tctgcggagc gccatcagct atattgagcg gctgcaggac ctgctgcacc ggctggatca
     1681 gcaggagaag atgcaggagc tgggggtgga ccccttcagc tacagaccca aacaagaaaa
     1741 tcttgagggt gcggatttcc tgcgcacctg cagctcccag tggccaagtg tttccgatca
     1801 ttccaggggg ctcgtgataa cggctaagga aggaggagca agtattgatt cgtcagcctc
     1861 gagtagcctt cgatgccttt cttccatcgt ggacagtatt tcctcggagg aacgcaaact
     1921 cccctgcgtg gaggaagtgg tggagaagta cccaactttc ttgtacaaag tggttgatat
     1981 cggtaagcct atccctaacc ctctcctcgg tctcgattct acgtagtaat gaactagtcc
     2041 gtaacttgaa agtatttcga tttcttggct ttatatatct tgtggaaagg acgagcccta
     2101 agtttttcag tggagcgaac gcgttaagtc gacaatcaac ctctggatta caaaatttgt
     2161 gaaagattga ctggtattct taactatgtt gctcctttta cgctatgtgg atacgctgct
     2221 ttaatgcctt tgtatcatgc tattgcttcc cgtatggctt tcattttctc ctccttgtat
     2281 aaatcctggt tgctgtctct ttatgaggag ttgtggcccg ttgtcaggca acgtggcgtg
     2341 gtgtgcactg tgtttgctga cgcaaccccc actggttggg gcattgccac cacctgtcag
     2401 ctcctttccg ggactttcgc tttccccctc cctattgcca cggcggaact catcgccgcc
     2461 tgccttgccc gctgctggac aggggctcgg ctgttgggca ctgacaattc cgtggtgttg
     2521 tcggggaaat catcgtcctt tccttggctg ctcgcctgtg ttgccacctg gattctgcgc
     2581 gggacgtcct tctgctacgt cccttcggcc ctcaatccag cggaccttcc ttcccgcggc
     2641 ctgctgccgg ctctgcggcc tcttccgcgt cttcgccttc gccctcagac gagtcggatc
     2701 tccctttggg ccgcctcccc gcgtcgactt taagaccaat gacttacaag gcagctgtag
     2761 atcttagcca ctttttaaaa gaaaaggggg gactggaagg gctaattcac tcccaacgaa
     2821 gacaagatct gctttttgct tgtactgggt ctctctggtt agaccagatc tgagcctggg
     2881 agctctctgg ctaactaggg aacccactgc ttaagcctca ataaagcttg ccttgagtgc
     2941 ttcaagtagt gtgtgcccgt ctgttgtgtg actctggtaa ctagagatcc ctcagaccct
     3001 tttagtcagt gtggaaaatc tctagcagta cgtatagtag ttcatgtcat cttattattc
     3061 agtatttata acttgcaaag aaatgaatat cagagagtga gaggaacttg tttattgcag
     3121 cttataatgg ttacaaataa agcaatagca tcacaaattt cacaaataaa gcattttttt
     3181 cactgcattc tagttgtggt ttgtccaaac tcatcaatgt atcttatcat gtctggctct
     3241 agctatcccg cccctaactc cgcccatccc gcccctaact ccgcccagtt ccgcccattc
     3301 tccgccccat ggctgactaa ttttttttat ttatgcagag gccgaggccg cctcggcctc
     3361 tgagctattc cagaagtagt gaggaggctt ttttggaggc ctagggacgt acccaattcg
     3421 ccctatagtg agtcgtatta cgcgcgctca ctggccgtcg ttttacaacg tcgtgactgg
     3481 gaaaaccctg gcgttaccca acttaatcgc cttgcagcac atcccccttt cgccagctgg
     3541 cgtaatagcg aagaggcccg caccgatcgc ccttcccaac agttgcgcag cctgaatggc
     3601 gaatgggacg cgccctgtag cggcgcatta agcgcggcgg gtgtggtggt tacgcgcagc
     3661 gtgaccgcta cacttgccag cgccctagcg cccgctcctt tcgctttctt cccttccttt
     3721 ctcgccacgt tcgccggctt tccccgtcaa gctctaaatc gggggctccc tttagggttc
     3781 cgatttagtg ctttacggca cctcgacccc aaaaaacttg attagggtga tggttcacgt
     3841 agtgggccat cgccctgata gacggttttt cgccctttga cgttggagtc cacgttcttt
     3901 aatagtggac tcttgttcca aactggaaca acactcaacc ctatctcggt ctattctttt
     3961 gatttataag ggattttgcc gatttcggcc tattggttaa aaaatgagct gatttaacaa
     4021 aaatttaacg cgaattttaa caaaatatta acgcttacaa tttaggtggc acttttcggg
     4081 gaaatgtgcg cggaacccct atttgtttat ttttctaaat acattcaaat atgtatccgc
     4141 tcatgagaca ataaccctga taaatgcttc aataatattg aaaaaggaag agtatgagta
     4201 ttcaacattt ccgtgtcgcc cttattccct tttttgcggc attttgcctt cctgtttttg
     4261 ctcacccaga aacgctggtg aaagtaaaag atgctgaaga tcagttgggt gcacgagtgg
     4321 gttacatcga actggatctc aacagcggta agatccttga gagttttcgc cccgaagaac
     4381 gttttccaat gatgagcact tttaaagttc tgctatgtgg cgcggtatta tcccgtattg
     4441 acgccgggca agagcaactc ggtcgccgca tacactattc tcagaatgac ttggttgagt
     4501 actcaccagt cacagaaaag catcttacgg atggcatgac agtaagagaa ttatgcagtg
     4561 ctgccataac catgagtgat aacactgcgg ccaacttact tctgacaacg atcggaggac
     4621 cgaaggagct aaccgctttt ttgcacaaca tgggggatca tgtaactcgc cttgatcgtt
     4681 gggaaccgga gctgaatgaa gccataccaa acgacgagcg tgacaccacg atgcctgtag
     4741 caatggcaac aacgttgcgc aaactattaa ctggcgaact acttactcta gcttcccggc
     4801 aacaattaat agactggatg gaggcggata aagttgcagg accacttctg cgctcggccc
     4861 ttccggctgg ctggtttatt gctgataaat ctggagccgg tgagcgtggg tctcgcggta
     4921 tcattgcagc actggggcca gatggtaagc cctcccgtat cgtagttatc tacacgacgg
     4981 ggagtcaggc aactatggat gaacgaaata gacagatcgc tgagataggt gcctcactga
     5041 ttaagcattg gtaactgtca gaccaagttt actcatatat actttagatt gatttaaaac
     5101 ttcattttta atttaaaagg atctaggtga agatcctttt tgataatctc atgaccaaaa
     5161 tcccttaacg tgagttttcg ttccactgag cgtcagaccc cgtagaaaag atcaaaggat
     5221 cttcttgaga tccttttttt ctgcgcgtaa tctgctgctt gcaaacaaaa aaaccaccgc
     5281 taccagcggt ggtttgtttg ccggatcaag agctaccaac tctttttccg aaggtaactg
     5341 gcttcagcag agcgcagata ccaaatactg ttcttctagt gtagccgtag ttaggccacc
     5401 acttcaagaa ctctgtagca ccgcctacat acctcgctct gctaatcctg ttaccagtgg
     5461 ctgctgccag tggcgataag tcgtgtctta ccgggttgga ctcaagacga tagttaccgg
     5521 ataaggcgca gcggtcgggc tgaacggggg gttcgtgcac acagcccagc ttggagcgaa
     5581 cgacctacac cgaactgaga tacctacagc gtgagctatg agaaagcgcc acgcttcccg
     5641 aagggagaaa ggcggacagg tatccggtaa gcggcagggt cggaacagga gagcgcacga
     5701 gggagcttcc agggggaaac gcctggtatc tttatagtcc tgtcgggttt cgccacctct
     5761 gacttgagcg tcgatttttg tgatgctcgt caggggggcg gagcctatgg aaaaacgcca
     5821 gcaacgcggc ctttttacgg ttcctggcct tttgctggcc ttttgctcac atgttctttc
     5881 ctgcgttatc ccctgattct gtggataacc gtattaccgc ctttgagtga gctgataccg
     5941 ctcgccgcag ccgaacgacc gagcgcagcg agtcagtgag cgaggaagcg gaagagcgcc
     6001 caatacgcaa accgcctctc cccgcgcgtt ggccgattca ttaatgcagc tggcacgaca
     6061 ggtttcccga ctggaaagcg ggcagtgagc gcaacgcaat taatgtgagt tagctcactc
     6121 attaggcacc ccaggcttta cactttatgc ttccggctcg tatgttgtgt ggaattgtga
     6181 gcggataaca atttcacaca ggaaacagct atgaccatga ttacgccaag cgcgcaatta
     6241 accctcacta aagggaacaa aagctggagc tgcaagctta atgtagtctt atgcaatact
     6301 cttgtagtct tgcaacatgg taacgatgag ttagcaacat gccttacaag gagagaaaaa
     6361 gcaccgtgca tgccgattgg tggaagtaag gtggtacgat cgtgccttat taggaaggca
     6421 acagacgggt ctgacatgga ttggacgaac cactgaattg ccgcattgca gagatattgt
     6481 atttaagtgc ctagctcgat acataaacgg gtctctctgg ttagaccaga tctgagcctg
     6541 ggagctctct ggctaactag ggaacccact gcttaagcct caataaagct tgccttgagt
     6601 gcttcaagta gtgtgtgccc gtctgttgtg tgactctggt aactagagat ccctcagacc
     6661 cttttagtca gtgtggaaaa tctctagcag tggcgcccga acagggactt gaaagcgaaa
     6721 gggaaaccag aggagctctc tcgacgcagg actcggcttg ctgaagcgcg cacggcaaga
     6781 ggcgaggggc ggcgactggt gagtacgcca aaaattttga ctagcggagg ctagaaggag
     6841 agagatgggt gcgagagcgt cagtattaag cgggggagaa ttagatcgcg atgggaaaaa
     6901 attcggttaa ggccaggggg aaagaaaaaa tataaattaa aacatatagt atgggcaagc
     6961 agggagctag aacgattcgc agttaatcct ggcctgttag aaacatcaga aggctgtaga
     7021 caaatactgg gacagctaca accatccctt cagacaggat cagaagaact tagatcatta
     7081 tataatacag tagcaaccct ctattgtgtg catcaaagga tagagataaa agacaccaag
     7141 gaagctttag acaagataga ggaagagcaa aacaaaagta agaccaccgc acagcaagcg
     7201 gccgctgatc ttcagacctg gaggaggaga tatgagggac aattggagaa gtgaattata
     7261 taaatataaa gtagtaaaaa ttgaaccatt aggagtagca cccaccaagg caaagagaag
     7321 agtggtgcag agagaaaaaa gagcagtggg aataggagct ttgttccttg ggttcttggg
     7381 agcagcagga agcactatgg gcgcagcgtc aatgacgctg acggtacagg ccagacaatt
     7441 attgtctggt atagtgcagc agcagaacaa tttgctgagg gctattgagg cgcaacagca
     7501 tctgttgcaa ctcacagtct ggggcatcaa gcagctccag gcaagaatcc tggctgtgga
     7561 aagataccta aaggatcaac agctcctggg gatttggggt tgctctggaa aactcatttg
     7621 caccactgct gtgccttgga atgctagttg gagtaataaa tctctggaac agatttggaa
     7681 tcacacgacc tggatggagt gggacagaga aattaacaat tacacaagct taatacactc
     7741 cttaattgaa gaatcgcaaa accagcaaga aaagaatgaa caagaattat tggaattaga
     7801 taaatgggca agtttgtgga attggtttaa cataacaaat tggctgtggt atataaaatt
     7861 attcataatg atagtaggag gcttggtagg tttaagaata gtttttgctg tactttctat
     7921 agtgaataga gttaggcagg gatattcacc attatcgttt cagacccacc tcccaacccc
     7981 gaggggaccc atgcattgca tctcaattag tcagcaacca ggtgtggaaa gtccccaggc
     8041 tccccagcag gcagaagtat gcaaagcatg cgtctcaatt agtcagcaac catagtcccg
     8101 cccctaactc cgcccatccc gcccctaact ccgcccagtt ccgcccattc tccgccccat
     8161 ggctgactaa ttttttttat ttatgcagag gccgaggccg cctcggcctc tgagctattc
     8221 cagaagtagt gaggaggctt ttttggaggc ctaggctttt gcaaaaagct ttctagaggt
     8281 accaccggag cttaccatga ccgagtacaa gcccacggtg cgcctcgcca cccgcgacga
     8341 cgtccccagg gccgtacgca ccctcgccgc cgcgttcgcc gactaccccg ccacgcgcca
     8401 caccgtcgat ccggaccgcc acatcgagcg ggtcaccgag ctgcaagaac tcttcctcac
     8461 gcgcgtcggg ctcgacatcg gcaaggtgtg ggtcgcggac gacggcgccg ccgtggcggt
     8521 ctggaccacg ccggagagcg tcgaagcggg ggcggtgttc gccgagatcg gcccgcgcat
     8581 ggccgagttg agcggttccc ggctggccgc gcagcaacag atggaaggcc tcctggcgcc
     8641 gcaccggccc aaggagcccg cgtggttcct ggccaccgtc ggcgtctcgc ccgaccacca
     8701 gggcaagggt ctgggcagcg ccgtcgtgct ccccggagtg gaggcggccg agcgcgccgg
     8761 ggtgcccgcc ttcctggaga cctccgcgcc ccgcaacctc cccttctacg agcggctcgg
     8821 cttcaccgtc accgccgacg tcgaggtgcc cgaaggaccg cgcacctggt gcatgacccg
     8881 caagcccggt gccagttaac gaattctaga tcttgagaca aatggcagta ttcatccaca
     8941 attttaaaag aaaagggggg attggggggt acagtgcagg ggaaagaata gtagacataa
     9001 tagcaacaga catacaaact aaagaattac aaaaacaaat tacaaaaatt caaaattttc
     9061 gggtttatta cagggacagc agagatccac tttggcgccg gctcgaggcc tgcaggtgca
     9121 aagatggata aagttttaaa cagagaggaa tctttgcagc taatggacct tctaggtctt
     9181 gaaaggagtg ggaatt
//
