LOCUS       sequence-353346-        2603 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  One-step assembly of custom RVD arrays to construct DdCBE, TALE, 
            etc..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2603)
  AUTHORS   Guo J, Zhang X, Chen X, Sun H, Dai Y, Wang J, Qian X, Tan L, Lou X, 
            Shen B
  TITLE     Precision modeling of mitochondrial diseases in zebrafish via 
            DdCBE-mediated mtDNA base editing.
  JOURNAL   Cell Discov. 2021 Sep 3;7(1):78. doi: 10.1038/s41421-021-00307-9.
  PUBMED    34480028
REFERENCE   2  (bases 1 to 2603)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell 
            Discov."; date: "2021-09-3"; volume: "7(1):78. doi"; pages: " 
            10.1038/s41421-021-00307-9"
COMMENT     Sequence Label: RVD-AG7
FEATURES             Location/Qualifiers
     source          1..2603
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     666..682
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     666..682
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     687..706
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        687..705
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             complement(880..2070)
                     /codon_start=1
                     /gene="tet"
                     /product="tetracycline efflux protein"
                     /label=TcR
                     /note="confers resistance to tetracycline"
                     /translation="MKSNNALIVILGTVTLDAVGIGLVMPVLPGLLRDIVHSDSIASHY
                     GVLLALYALMQFLCAPVLGALSDRFGRRPVLLASLLGATIDYAIMATTPVLWILYAGRI
                     VAGITGATGAVAGAYIADITDGEDRARHFGLMSACFGVGMVAGPVAGGLLGAISLHAPF
                     LAAAVLNGLNLLLGCFLMQESHKGERRPMPLRAFNPVSSFRWARGMTIVAALMTVFFIM
                     QLVGQVPAALWVIFGEDRFRWSATMIGLSLAVFGILHALAQAFVTGPATKRFGEKQAII
                     AGMAADALGYVLLAFATRGWMAFPIMILLASGGIGMPALQAMLSRQVDDDHQGQLQGSL
                     AALTSLTSIIGPLIVTAIYAASASTWNGLAWIVGAALYLVCLPALRRGAWSRATST"
     primer_bind     1699..1718
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
     promoter        complement(join(2118..2123,2124..2140,2141..2146))
                     /label=tet promoter
                     /note="E. coli promoter for tetracycline efflux protein 
                     gene"
     rep_origin      complement(join(2273..2603,1..258))
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(2353..2372)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 gacttatcgc cactggcagc agccactggt aacaggatta gcagagcgag gtatgtaggc
       61 ggtgctacag agttcttgaa gtggtggcct aactacggct acactagaag aacagtattt
      121 ggtatctgcg ctctgctgaa gccagttacc ttcggaaaaa gagttggtag ctcttgatcc
      181 ggcaaacaaa ccaccgctgg tagcggtggt ttttttgttt gcaagcagca gattacgcgc
      241 agaaaaaaag gatctcaaga agatcctttg atcttttcta cggggtctga cgctcagtgg
      301 aacgacgcgt aactcacgtt aagggatttt ggtcatgggt ggctcgacca gatatggtct
      361 cgagccagta atattggtgg caaacaggct cttgagacgg ttcagcgcct ccttccagtt
      421 ctctgtcaag cccacggact caccccagat caagttgtag cgattgctag taacaatggt
      481 ggcaaacagg ctcttgaaac cgtacagcgc ctactgccag ttctctgtca agcccacggt
      541 ctgactccgg agcaagttgt agcgattgca agttgagacc agctttggtc tagaggaggc
      601 atgatgatat atttttatct tgtgcaatgt aacatcagag attttgagac acgggccaga
      661 gctgccagga aacagctatg accatgtaat acgactcact ataggggata ttttattttc
      721 tcccaatcag gcttgatccc cagtaagtca aaaaatagct cgacatactg ttcttccccg
      781 atcaagggtt ggtttgcgca ttcacagttc tccgcaagaa ttgattggct ccaattcttg
      841 gagtggtgaa tccgttagcg aggtgccgcc ggcttccatt caggtcgagg tggcccggct
      901 ccatgcaccg cgacgcaacg cggggaggca gacaaggtat agggcggcgc ctacaatcca
      961 tgccaacccg ttccatgtgc tcgccgaggc ggcataaatc gccgtgacga tcagcggtcc
     1021 aatgatcgaa gttaggctgg taagagccgc gagcgatcct tgaagctgtc cctgatggtc
     1081 gtcatctacc tgcctggaca gcatggcctg caacgcgggc atcccgatgc cgccggaagc
     1141 gagaagaatc ataatgggga aggccatcca gcctcgcgtc gcgaacgcca gcaagacgta
     1201 gcccagcgcg tcggccgcca tgccggcgat aatggcctgc ttctcgccga aacgtttggt
     1261 ggcgggacca gtgacgaagg cttgagcgag ggcgtgcaag attccgaata ccgcaagcga
     1321 caggccgatc atcgtcgcgc tccagcgaaa gcggtcctcg ccgaaaatga cccagagcgc
     1381 tgccggcacc tgtcctacga gttgcatgat aaagaagaca gtcataagtg cggcgacgat
     1441 agtcatgccc cgcgcccacc ggaaggagct gactgggttg aaggctctca agggcatggg
     1501 tcggcgctct cccttatgcg actcctgcat taggaagcag cccagtagta ggttgaggcc
     1561 gttgagcacc gccgccgcaa ggaatggtgc atgtaaggag atggcgccca acagtccccc
     1621 ggccacgggg cctgccacca tacccacgcc gaaacaagcg ctcatgagcc cgaagtggcg
     1681 agcccgatct tccccatcgg tgatgtcggc gatataggcg ccagcaaccg cacctgtggc
     1741 gccggtgatg ccggccacga tgcgtccggc gtagagaatc cacaggacgg gtgtggtcgc
     1801 catgatcgcg tagtcgatag tggctccaag tagcgaagcg agcaggactg ggcggcggcc
     1861 aaagcggtcg gacagtgctc cgagaacggg tgcgcataga aattgcatca acgcatatag
     1921 cgctagcagc acgccatagt gactggcgat gctgtcggaa tggacgatat cccgcaagag
     1981 gcccggcagt accggcataa ccaagcctat gcctacagca tccagggtga cggtgccgag
     2041 gatgacgatg agcgcattgt tagatttcat acacggtgcc tgactgcgtt agcaatttaa
     2101 ctgtgataaa ctaccgcatt aaagctaatc gatgataagc tgtcaaacat gagaattaat
     2161 tcatgttctt tcctgcgtta tcccctgatt ctgtggataa cgcaggaaag aacatgtgag
     2221 caaaaggcca gcaaaaggcc aggaaccgta aaaaggccgc gttgctggcg tttttccata
     2281 ggctccgccc ccctgacgag catcacaaaa atcgacgctc aagtcagagg tggcgaaacc
     2341 cgacaggact ataaagatac caggcgtttc cccctggaag ctccctcgtg cgctctcctg
     2401 ttccgaccct gccgcttacc ggatacctgt ccgcctttct cccttcggga agcgtggcgc
     2461 tttctcatag ctcacgctgt aggtatctca gttcggtgta ggtcgttcgc tccaagctgg
     2521 gctgtgtgca cgaacccccc gttcagcccg accgctgcgc cttatccggt aactatcgtc
     2581 ttgagtccaa cccggtaaga cac
//
