LOCUS       sequence-353347-        2600 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  One-step assembly of custom RVD arrays to construct DdCBE, TALE, 
            etc..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2600)
  AUTHORS   Guo J, Zhang X, Chen X, Sun H, Dai Y, Wang J, Qian X, Tan L, Lou X, 
            Shen B
  TITLE     Precision modeling of mitochondrial diseases in zebrafish via 
            DdCBE-mediated mtDNA base editing.
  JOURNAL   Cell Discov. 2021 Sep 3;7(1):78. doi: 10.1038/s41421-021-00307-9.
  PUBMED    34480028
REFERENCE   2  (bases 1 to 2600)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell 
            Discov."; date: "2021-09-3"; volume: "7(1):78. doi"; pages: " 
            10.1038/s41421-021-00307-9"
COMMENT     Sequence Label: RVD-AG8
FEATURES             Location/Qualifiers
     source          1..2600
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        complement(join(407..412,413..429,430..435))
                     /label=tet promoter
                     /note="E. coli promoter for tetracycline efflux protein 
                     gene"
     rep_origin      complement(562..1150)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(642..661)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     1555..1571
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     1555..1571
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     1576..1595
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        1576..1594
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             complement(join(1769..2600,1..359))
                     /codon_start=1
                     /gene="tet"
                     /product="tetracycline efflux protein"
                     /label=TcR
                     /note="confers resistance to tetracycline"
                     /translation="MKSNNALIVILGTVTLDAVGIGLVMPVLPGLLRDIVHSDSIASHY
                     GVLLALYALMQFLCAPVLGALSDRFGRRPVLLASLLGATIDYAIMATTPVLWILYAGRI
                     VAGITGATGAVAGAYIADITDGEDRARHFGLMSACFGVGMVAGPVAGGLLGAISLHAPF
                     LAAAVLNGLNLLLGCFLMQESHKGERRPMPLRAFNPVSSFRWARGMTIVAALMTVFFIM
                     QLVGQVPAALWVIFGEDRFRWSATMIGLSLAVFGILHALAQAFVTGPATKRFGEKQAII
                     AGMAADALGYVLLAFATRGWMAFPIMILLASGGIGMPALQAMLSRQVDDDHQGQLQGSL
                     AALTSLTSIIGPLIVTAIYAASASTWNGLAWIVGAALYLVCLPALRRGAWSRATST"
     primer_bind     join(2588..2600,1..7)
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
ORIGIN
        1 atataggcgc cagcaaccgc acctgtggcg ccggtgatgc cggccacgat gcgtccggcg
       61 tagagaatcc acaggacggg tgtggtcgcc atgatcgcgt agtcgatagt ggctccaagt
      121 agcgaagcga gcaggactgg gcggcggcca aagcggtcgg acagtgctcc gagaacgggt
      181 gcgcatagaa attgcatcaa cgcatatagc gctagcagca cgccatagtg actggcgatg
      241 ctgtcggaat ggacgatatc ccgcaagagg cccggcagta ccggcataac caagcctatg
      301 cctacagcat ccagggtgac ggtgccgagg atgacgatga gcgcattgtt agatttcata
      361 cacggtgcct gactgcgtta gcaatttaac tgtgataaac taccgcatta aagctaatcg
      421 atgataagct gtcaaacatg agaattaatt catgttcttt cctgcgttat cccctgattc
      481 tgtggataac gcaggaaaga acatgtgagc aaaaggccag caaaaggcca ggaaccgtaa
      541 aaaggccgcg ttgctggcgt ttttccatag gctccgcccc cctgacgagc atcacaaaaa
      601 tcgacgctca agtcagaggt ggcgaaaccc gacaggacta taaagatacc aggcgtttcc
      661 ccctggaagc tccctcgtgc gctctcctgt tccgaccctg ccgcttaccg gatacctgtc
      721 cgcctttctc ccttcgggaa gcgtggcgct ttctcatagc tcacgctgta ggtatctcag
      781 ttcggtgtag gtcgttcgct ccaagctggg ctgtgtgcac gaaccccccg ttcagcccga
      841 ccgctgcgcc ttatccggta actatcgtct tgagtccaac ccggtaagac acgacttatc
      901 gccactggca gcagccactg gtaacaggat tagcagagcg aggtatgtag gcggtgctac
      961 agagttcttg aagtggtggc ctaactacgg ctacactaga agaacagtat ttggtatctg
     1021 cgctctgctg aagccagtta ccttcggaaa aagagttggt agctcttgat ccggcaaaca
     1081 aaccaccgct ggtagcggtg gtttttttgt ttgcaagcag cagattacgc gcagaaaaaa
     1141 aggatctcaa gaagatcctt tgatcttttc tacggggtct gacgctcagt ggaacgacgc
     1201 gtaactcacg ttaagggatt ttggtcatgg gtggctcgac cagatatggt ctcaggaagc
     1261 aatcgctaca acttgctccg gagtcagacc gtgggcttga cagagaactg gcagtaggcg
     1321 ctgtacggtt tcaagagcct gtttgccacc attgttacta gcaatcgcta caacttgatc
     1381 tggggtgagt ccgtgggctt gacagagaac tggaaggagg cgctgaaccg tctcaagagc
     1441 ctgtttgcca ccaatattac ttcgagacca gctttggtct agaggaggca tgatgatata
     1501 tttttatctt gtgcaatgta acatcagaga ttttgagaca cgggccagag ctgccaggaa
     1561 acagctatga ccatgtaata cgactcacta taggggatat tttattttct cccaatcagg
     1621 cttgatcccc agtaagtcaa aaaatagctc gacatactgt tcttccccga tcaagggttg
     1681 gtttgcgcat tcacagttct ccgcaagaat tgattggctc caattcttgg agtggtgaat
     1741 ccgttagcga ggtgccgccg gcttccattc aggtcgaggt ggcccggctc catgcaccgc
     1801 gacgcaacgc ggggaggcag acaaggtata gggcggcgcc tacaatccat gccaacccgt
     1861 tccatgtgct cgccgaggcg gcataaatcg ccgtgacgat cagcggtcca atgatcgaag
     1921 ttaggctggt aagagccgcg agcgatcctt gaagctgtcc ctgatggtcg tcatctacct
     1981 gcctggacag catggcctgc aacgcgggca tcccgatgcc gccggaagcg agaagaatca
     2041 taatggggaa ggccatccag cctcgcgtcg cgaacgccag caagacgtag cccagcgcgt
     2101 cggccgccat gccggcgata atggcctgct tctcgccgaa acgtttggtg gcgggaccag
     2161 tgacgaaggc ttgagcgagg gcgtgcaaga ttccgaatac cgcaagcgac aggccgatca
     2221 tcgtcgcgct ccagcgaaag cggtcctcgc cgaaaatgac ccagagcgct gccggcacct
     2281 gtcctacgag ttgcatgata aagaagacag tcataagtgc ggcgacgata gtcatgcccc
     2341 gcgcccaccg gaaggagctg actgggttga aggctctcaa gggcatgggt cggcgctctc
     2401 ccttatgcga ctcctgcatt aggaagcagc ccagtagtag gttgaggccg ttgagcaccg
     2461 ccgccgcaag gaatggtgca tgtaaggaga tggcgcccaa cagtcccccg gccacggggc
     2521 ctgccaccat acccacgccg aaacaagcgc tcatgagccc gaagtggcga gcccgatctt
     2581 ccccatcggt gatgtcggcg
//
