LOCUS       sequence-35336-e         849 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 849)
  AUTHORS   Hussein R, Lim HN
  TITLE     Disruption of small RNA signaling caused by competition for Hfq.
  JOURNAL   Proc Natl Acad Sci U S A. 2011 Jan 18;108(3):1110-5. doi: 
            10.1073/pnas.1010082108. Epub 2010 Dec 28.
  PUBMED    21189298
REFERENCE   2  (bases 1 to 849)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1073/pnas.1010082108"; journalName: "Proc Natl Acad Sci U S A"; 
            date: "2011-01-18- 18"; volume: "108"; issue: "3"; pages: "1110-5"
COMMENT     Sequence Label: HL 4179 (=HL 865 + pHL 1381)
FEATURES             Location/Qualifiers
     source          1..849
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    209..227
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    234..252
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     primer_bind     complement(273..291)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        359..463
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(682..701)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
ORIGIN
        1 cttctccttt acgcatgcta tcatgaactt tcagcgtatt ctgactcata aggtggctcc
       61 tacccgtgat cccttgacgg aacattcaag caaaagcctg gttccgccga tttatcgctg
      121 cggcaaataa cgcagcgggt ttacggattt ccccttgtaa cgaatttcaa aatgcaagcg
      181 tgttgaactg gttccggtgt cgacagtatc tctatcactg atagggatgt caatctctat
      241 cactgatagg gactcgaggt gaagacgaaa gggcctcgtg atacgcctat ttttataggt
      301 taatgtcatg ataataatgg tttcttagac gtcaggtggc acttttcggg gaaatgtgcg
      361 cggaacccct atttgtttat ttttctaaat acattcaaat atgtatccgc tcatgagaca
      421 ataaccctga taaatgcttc aataatattg aaaaaggaag agtatgagta ttcaacattt
      481 ccgtgtcgcc cttattccct tttttgcggc attttgcctt cctgtttttg ctcacccaga
      541 aacgctggtg aaagtaaaag atgctgaaga tcagttgggt gcacgagtgg gttacatcga
      601 actggatctc aacagcggta agatccttga gagttttcgc cccgaagaac gttttccaat
      661 gatgagcact tttaaagttc tgctatgtgg cgcggtatta tcccgtattg acgccgggca
      721 agagcaactc ggtcgccgca tacactattc tcagaatgac ttggttgagt actcaccagt
      781 cacagaaaag catcttacgg atggcatgac agtaagagaa ttatgcagtg ctgccataac
      841 catgagtga
//
