LOCUS       sequence-353363-        7981 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  retrovirus production.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7981)
  AUTHORS   Lomonosov M, Meziane el K, Ye H, Nelson DE, Randle SJ, Laman H
  TITLE     Expression of Fbxo7 in haematopoietic progenitor cells cooperates 
            with p53 loss to promote lymphomagenesis.
  JOURNAL   PLoS One. 2011;6(6):e21165. doi: 10.1371/journal.pone.0021165. Epub 
            2011 Jun 17.
  PUBMED    21695055
REFERENCE   2  (bases 1 to 7981)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2011"; volume: "6(6):e21165. doi"; pages: " 
            10.1371/journal.pone.0021165. Epub 2011 Jun 17"
COMMENT     Sequence Label: MSCV-Fbxo7-IRES-GFP
FEATURES             Location/Qualifiers
     source          1..7981
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     LTR             112..628
                     /label=5' LTR
                     /note="5' long terminal repeat from murine embryonic stem 
                     cell virus"
     misc_feature    692..1033
                     /label=MESV Psi
                     /note="packaging signal of murine embryonic stem cell 
                     virus"
     CDS             1100..1516
                     /label=gag (truncated)
                     /note="truncated Moloney murine leukemia virus (MMLV) gag 
                     gene lacking the start codon"
     primer_bind     1446..1468
                     /label=pLXSN 5'
                     /note="Murine stem cell virus, forward primer. Also called 
                     MSCV"
     primer_bind     1484..1500
                     /label=pBABE 5'
                     /note="Psi packaging signal, forward primer for pBABE 
                     vectors"
     CDS             1539..1571
                     /codon_start=1
                     /product="leader peptide from bacteriophage T7 gene 10"
                     /label=T7 tag (gene 10 leader)
                     /note="promotes efficient translation in E. coli"
                     /translation="MASMTGGQQMG"
     misc_feature    3085..3652
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     misc_feature    3102..3654
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(3246..3263)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     3473..3492
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             join(3666..3668,3669..3671,3672..4385)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     ANFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(3711..3732)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(3972..3991)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     4319..4340
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     LTR             4487..5001
                     /label=3' LTR
                     /note="3' long terminal repeat from murine embryonic stem 
                     cell virus"
     protein_bind    5163..5179
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(5187..5193,5194..5211,5212..5217))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    5232..5253
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(5370..5387)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(5541..6129)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(5621..5640)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(6300..7091,7092..7160))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     6923..6942
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(7161..7265)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     7333..7351
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(7389..7411)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     7511..7530
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     7724..7746
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     7874..7893
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
ORIGIN
        1 atcagtggtc caggctctag ttttgactca acaatatcac cagctgaagc ctatagagta
       61 cgagccatag ataaaataaa agattttatt tagtctccag aaaaaggggg gaatgaaaga
      121 ccccacctgt aggtttggca agctagctta agtaacgcca ttttgcaagg catggaaaat
      181 acataactga gaatagagaa gttcagatca aggttaggaa cagagagaca gcagaatatg
      241 ggccaaacag gatatctgtg gtaagcagtt cctgccccgg ctcagggcca agaacagatg
      301 gtccccagat gcggtcccgc cctcagcagt ttctagagaa ccatcagatg tttccagggt
      361 gccccaagga cctgaaatga ccctgtgcct tatttgaact aaccaatcag ttcgcttctc
      421 gcttctgttc gcgcgcttct gctccccgag ctcaataaaa gagcccacaa cccctcactc
      481 ggcgcgccag tcctccgata gactgcgtcg cccgggtacc cgtattccca ataaagcctc
      541 ttgctgtttg catccgaatc gtggactcgc tgatccttgg gagggtctcc tcagattgat
      601 tgactgccca cctcgggggt ctttcatttg gaggttccac cgagatttgg agacccctgc
      661 ccagggacca ccgacccccc cgccgggagg taagctggcc agcggtcgtt tcgtgtctgt
      721 ctctgtcttt gtgcgtgttt gtgccggcat ctaatgtttg cgcctgcgtc tgtactagtt
      781 agctaactag ctctgtatct ggcggacccg tggtggaact gacgagttct gaacacccgg
      841 ccgcaaccct gggagacgtc ccagggactt tgggggccgt ttttgtggcc cgacctgagg
      901 aagggagtcg atgtggaatc cgaccccgtc aggatatgtg gttctggtag gagacgagaa
      961 cctaaaacag ttcccgcctc cgtctgaatt tttgctttcg gtttggaacc gaagccgcgc
     1021 gtcttgtctg ctgcagcgct gcagcatcgt tctgtgttgt ctctgtctga ctgtgtttct
     1081 gtatttgtct gaaaattagg gccagactgt taccactccc ttaagtttga ccttaggtca
     1141 ctggaaagat gtcgagcgga tcgctcacaa ccagtcggta gatgtcaaga agagacgttg
     1201 ggttaccttc tgctctgcag aatggccaac ctttaacgtc ggatggccgc gagacggcac
     1261 ctttaaccga gacctcatca cccaggttaa gatcaaggtc ttttcacctg gcccgcatgg
     1321 acacccagac caggtcccct acatcgtgac ctgggaagcc ttggcttttg acccccctcc
     1381 ctgggtcaag ccctttgtac accctaagcc tccgcctcct cttcctccat ccgccccgtc
     1441 tctccccctt gaacctcctc gttcgacccc gcctcgatcc tccctttatc cagccctcac
     1501 tccttctcta ggcgccggaa ttagatctct cgagcaccat ggctagcatg actggtggac
     1561 agcaaatggg tggatccact agtaacggcc gccagtgtgc tggaattctt atgaggctgc
     1621 gggtgcggct tctgaagcgg acctggccgc tggaggtgcc cgagacggag ccgacgctgg
     1681 ggcatttgcg ctcgcacctg aggcagtccc tgctgtgcac ctgggggtac agttctaata
     1741 cccgatttac aattacattg aactacaagg atcccctcac tggagatgaa gagaccttgg
     1801 cttcatatgg gattgtttct ggggacttga tatgtttgat tcttcaagat gacattccag
     1861 cgcctaatat accttcatcc acagattcag agcattcttc actccagaat aatgagcaac
     1921 cctctttggc caccagctcc aatcagacta gcatgcagga tgaacaacca agtgattcat
     1981 tccaaggaca ggcagcccag tctggtgttt ggaatgacga cagtatgtta gggcctagtc
     2041 aaaattttga agctgagtca attcaagata atgcgcatat ggcagagggc acaggtttct
     2101 atccctcaga acccatgctc tgtagtgaat cggtggaagg gcaagtgcca cattcattag
     2161 agaccttgta tcaatcagct gactgttctg atgccaatga tgccttgata gtgttgatac
     2221 atcttctcat gttggagtca ggttacatac ctcagggcac cgaagccaaa gcactgtcca
     2281 tgccggagaa gtggaagttg agcggggtgt ataagctgca gtacatgcat cctctctgcg
     2341 agggcagctc cgctactctc acctgtgtgc ctttgggaaa cctgattgtt gtaaatgcta
     2401 cactaaaaat caacaatgag attagaagtg tgaaaagatt gcagctgcta ccagaatctt
     2461 ttatttgcaa agagaaacta ggggaaaatg tagccaacat atacaaagat cttcagaaac
     2521 tctctcgcct ctttaaagac cagctggtgt atcctcttct ggcttttacc cgacaagcac
     2581 tgaacctacc agatgtattt gggttggtcg tcgacccact cctgtggagg tttttatatc
     2641 tgcgtgattt tcgagacaat actgtcagag ttcaagacac agattggaaa gaactgtaca
     2701 ggaagaggca catacaaaga aaagaatccc cgaaagggcg gtttgtgatg ctcctgccat
     2761 cgtcaactca caccattcca ttctatccca accccttgca ccctaggcca tttcctagct
     2821 cccgccttcc tccaggaatt atcgggggtg aatatgacca aagaccaaca cttccctatg
     2881 ttggagaccc aatcagttca ctcattcctg gtcctgggga gacgcccagc cagtttcctc
     2941 cactgagacc acgctttgat ccagttggcc cacttccagg acctaacccc atcttgccag
     3001 ggcgaggcgg ccccaatgac agatttccct ttagacccag caggggtcgg ccaactgatg
     3061 gccggctgtc attcatgtga gaattccgcc ccccccccct aacgttactg gccgaagccg
     3121 cttggaataa ggccggtgtg cgtttgtcta tatgttattt tccaccatat tgccgtcttt
     3181 tggcaatgtg agggcccgga aacctggccc tgtcttcttg acgagcattc ctaggggtct
     3241 ttcccctctc gccaaaggaa tgcaaggtct gttgaatgtc gtgaaggaag cagttcctct
     3301 ggaagcttct tgaagacaaa caacgtctgt agcgaccctt tgcaggcagc ggaacccccc
     3361 acctggcgac aggtgcctct gcggccaaaa gccacgtgta taagatacac ctgcaaaggc
     3421 ggcacaaccc cagtgccacg ttgtgagttg gatagttgtg gaaagagtca aatggctctc
     3481 ctcaagcgta ttcaacaagg ggctgaagga tgcccagaag gtaccccatt gtatgggatc
     3541 tgatctgggg cctcggtgca catgctttac atgtgtttag tcgaggttaa aaaaacgtct
     3601 aggccccccg aaccacgggg acgtggtttt cctttgaaaa acacgatgat aatatggcca
     3661 caaccatggt gagcaagggc gaggagctgt tcaccggggt ggtgcccatc ctggtcgagc
     3721 tggacggcga cgtaaacggc cacaagttca gcgtgtctgg cgagggcgag ggcgatgcca
     3781 cctacggcaa gctgaccctg aagttcatct gcaccaccgg caagctgccc gtgccctggc
     3841 ccaccctcgt gaccaccctg acctacggcg tgcagtgctt cagccgctac cccgaccaca
     3901 tgaagcagca cgacttcttc aagtccgcca tgcccgaagg ctacgtccag gagcgcacca
     3961 tcttcttcaa ggacgacggc aactacaaga cccgcgccga ggtgaagttc gagggcgaca
     4021 ccctggtgaa ccgcatcgag ctgaagggca tcgacttcaa ggaggacggc aacatcctgg
     4081 ggcacaagct ggagtacaac tacaacagcc acaacgtcta tatcatggcc gacaagcaga
     4141 agaacggcat caaggcgaac ttcaagatcc gccacaacat cgaggacggc agcgtgcagc
     4201 tcgccgacca ctaccagcag aacaccccca tcggcgacgg ccccgtgctg ctgcccgaca
     4261 accactacct gagcacccag tccgccctga gcaaagaccc caacgagaag cgcgatcaca
     4321 tggtcctgct ggagttcgtg accgccgccg ggatcactct cggcatggac gagctgtaca
     4381 agtaatgaat taattaagaa ttatcaagct tatcgatacc gtcgacctgc agccaagctt
     4441 atcgataaaa taaaagattt tatttagtct ccagaaaaag gggggaatga aagaccccac
     4501 ctgtaggttt ggcaagctag cttaagtaac gccattttgc aaggcatgga aaatacataa
     4561 ctgagaatag agaagttcag atcaaggtta ggaacagaga gacagcagaa tatgggccaa
     4621 acaggatatc tgtggtaagc agttcctgcc ccggctcagg gccaagaaca gatggtcccc
     4681 agatgcggtc ccgccctcag cagtttctag agaaccatca gatgtttcca gggtgcccca
     4741 aggacctgaa atgaccctgt gccttatttg aactaaccaa tcagttcgct tctcgcttct
     4801 gttcgcgcgc ttctgctccc cgagctcaat aaaagagccc acaacccctc actcggcgcg
     4861 ccagtcctcc gatagactgc gtcgcccggg tacccgtgta tccaataaac cctcttgcag
     4921 ttgcatccga cttgtggtct cgctgttcct tgggagggtc tcctctgagt gattgactac
     4981 ccgtcagcgg gggtctttca tgggtaacag tttcttgaag ttggagaaca acattctgag
     5041 ggtaggagtc gaatattaag taatcctgac tcaattagcc actgttttga atccacatac
     5101 tccaatactc ctgaaatagt tcattatgga cagcgcagaa agagctgggg agaattgtga
     5161 aattgttatc cgctcacaat tccacacaac atacgagccg gaagcataaa gtgtaaagcc
     5221 tggggtgcct aatgagtgag ctaactcaca ttaattgcgt tgcgctcact gcccgctttc
     5281 cagtcgggaa acctgtcgtg ccagctgcat taatgaatcg gccaacgcgc ggggagaggc
     5341 ggtttgcgta ttgggcgctc ttccgcttcc tcgctcactg actcgctgcg ctcggtcgtt
     5401 cggctgcggc gagcggtatc agctcactca aaggcggtaa tacggttatc cacagaatca
     5461 ggggataacg caggaaagaa catgtgagca aaaggccagc aaaaggccag gaaccgtaaa
     5521 aaggccgcgt tgctggcgtt tttccatagg ctccgccccc ctgacgagca tcacaaaaat
     5581 cgacgctcaa gtcagaggtg gcgaaacccg acaggactat aaagatacca ggcgtttccc
     5641 cctggaagct ccctcgtgcg ctctcctgtt ccgaccctgc cgcttaccgg atacctgtcc
     5701 gcctttctcc cttcgggaag cgtggcgctt tctcatagct cacgctgtag gtatctcagt
     5761 tcggtgtagg tcgttcgctc caagctgggc tgtgtgcacg aaccccccgt tcagcccgac
     5821 cgctgcgcct tatccggtaa ctatcgtctt gagtccaacc cggtaagaca cgacttatcg
     5881 ccactggcag cagccactgg taacaggatt agcagagcga ggtatgtagg cggtgctaca
     5941 gagttcttga agtggtggcc taactacggc tacactagaa gaacagtatt tggtatctgc
     6001 gctctgctga agccagttac cttcggaaaa agagttggta gctcttgatc cggcaaacaa
     6061 accaccgctg gtagcggtgg tttttttgtt tgcaagcagc agattacgcg cagaaaaaaa
     6121 ggatctcaag aagatccttt gatcttttct acggggtctg acgctcagtg gaacgaaaac
     6181 tcacgttaag ggattttggt catgagatta tcaaaaagga tcttcaccta gatcctttta
     6241 aattaaaaat gaagttttaa atcaatctaa agtatatatg agtaaacttg gtctgacagt
     6301 taccaatgct taatcagtga ggcacctatc tcagcgatct gtctatttcg ttcatccata
     6361 gttgcctgac tccccgtcgt gtagataact acgatacggg agggcttacc atctggcccc
     6421 agtgctgcaa tgataccgcg agacccacgc tcaccggctc cagatttatc agcaataaac
     6481 cagccagccg gaagggccga gcgcagaagt ggtcctgcaa ctttatccgc ctccatccag
     6541 tctattaatt gttgccggga agctagagta agtagttcgc cagttaatag tttgcgcaac
     6601 gttgttgcca ttgctacagg catcgtggtg tcacgctcgt cgtttggtat ggcttcattc
     6661 agctccggtt cccaacgatc aaggcgagtt acatgatccc ccatgttgtg caaaaaagcg
     6721 gttagctcct tcggtcctcc gatcgttgtc agaagtaagt tggccgcagt gttatcactc
     6781 atggttatgg cagcactgca taattctctt actgtcatgc catccgtaag atgcttttct
     6841 gtgactggtg agtactcaac caagtcattc tgagaatagt gtatgcggcg accgagttgc
     6901 tcttgcccgg cgtcaatacg ggataatacc gcgccacata gcagaacttt aaaagtgctc
     6961 atcattggaa aacgttcttc ggggcgaaaa ctctcaagga tcttaccgct gttgagatcc
     7021 agttcgatgt aacccactcg tgcacccaac tgatcttcag catcttttac tttcaccagc
     7081 gtttctgggt gagcaaaaac aggaaggcaa aatgccgcaa aaaagggaat aagggcgaca
     7141 cggaaatgtt gaatactcat actcttcctt tttcaatatt attgaagcat ttatcagggt
     7201 tattgtctca tgagcggata catatttgaa tgtatttaga aaaataaaca aataggggtt
     7261 ccgcgcacat ttccccgaaa agtgccacct gacgtctaag aaaccattat tatcatgaca
     7321 ttaacctata aaaataggcg tatcacgagg ccctttcgtc tcgcgcgttt cggtgatgac
     7381 ggtgaaaacc tctgacacat gcagctcccg gagacggtca cagcttgtct gtaagcggat
     7441 gccgggagca gacaagcccg tcagggcgcg tcagcgggtg ttggcgggtg tcggggctgg
     7501 cttaactatg cggcatcaga gcagattgta ctgagagtgc accatatgcg gtgtgaaata
     7561 ccgcacagat gcgtaaggag aaaataccgc atcaggcgcc attcgccatt caggctgcgc
     7621 aactgttggg aagggcgatc ggtgcgggcc tcttcgctat tacgccagct ggcgaaaggg
     7681 ggatgtgctg caaggcgatt aagttgggta acgccagggt tttcccagtc acgacgttgt
     7741 aaaacgacgg cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca
     7801 cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc
     7861 gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg
     7921 tgatgccggc cacgatgcgt ccggcgtaga ggcgattagt ccaatttgtt aaagacagga
     7981 t
//
