LOCUS       sequence-353364-        8080 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  retrovirus production.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8080)
  AUTHORS   Lomonosov M, Meziane el K, Ye H, Nelson DE, Randle SJ, Laman H
  TITLE     Expression of Fbxo7 in haematopoietic progenitor cells cooperates 
            with p53 loss to promote lymphomagenesis.
  JOURNAL   PLoS One. 2011;6(6):e21165. doi: 10.1371/journal.pone.0021165. Epub 
            2011 Jun 17.
  PUBMED    21695055
REFERENCE   2  (bases 1 to 8080)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2011"; volume: "6(6):e21165. doi"; pages: " 
            10.1371/journal.pone.0021165. Epub 2011 Jun 17"
COMMENT     Sequence Label: MSCV-Fbxo7(Delta-F-box)-IRES-GFP
FEATURES             Location/Qualifiers
     source          1..8080
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     LTR             112..628
                     /label=5' LTR
                     /note="5' long terminal repeat from murine embryonic stem 
                     cell virus"
     misc_feature    692..1033
                     /label=MESV Psi
                     /note="packaging signal of murine embryonic stem cell 
                     virus"
     CDS             1100..1516
                     /label=gag (truncated)
                     /note="truncated Moloney murine leukemia virus (MMLV) gag 
                     gene lacking the start codon"
     primer_bind     1446..1468
                     /label=pLXSN 5'
                     /note="Murine stem cell virus, forward primer. Also called 
                     MSCV"
     primer_bind     1484..1500
                     /label=pBABE 5'
                     /note="Psi packaging signal, forward primer for pBABE 
                     vectors"
     CDS             1539..1571
                     /codon_start=1
                     /product="leader peptide from bacteriophage T7 gene 10"
                     /label=T7 tag (gene 10 leader)
                     /note="promotes efficient translation in E. coli"
                     /translation="MASMTGGQQMG"
     misc_feature    3184..3751
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     misc_feature    3201..3753
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(3345..3362)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     3572..3591
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             join(3765..3767,3768..3770,3771..4484)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     ANFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(3810..3831)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(4071..4090)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     4418..4439
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     LTR             4586..5100
                     /label=3' LTR
                     /note="3' long terminal repeat from murine embryonic stem 
                     cell virus"
     protein_bind    5262..5278
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(5286..5292,5293..5310,5311..5316))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    5331..5352
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(5469..5486)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(5640..6228)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(5720..5739)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(6399..7190,7191..7259))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     7022..7041
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(7260..7364)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     7432..7450
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(7488..7510)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     7610..7629
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     7823..7845
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     7973..7992
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
ORIGIN
        1 atcagtggtc caggctctag ttttgactca acaatatcac cagctgaagc ctatagagta
       61 cgagccatag ataaaataaa agattttatt tagtctccag aaaaaggggg gaatgaaaga
      121 ccccacctgt aggtttggca agctagctta agtaacgcca ttttgcaagg catggaaaat
      181 acataactga gaatagagaa gttcagatca aggttaggaa cagagagaca gcagaatatg
      241 ggccaaacag gatatctgtg gtaagcagtt cctgccccgg ctcagggcca agaacagatg
      301 gtccccagat gcggtcccgc cctcagcagt ttctagagaa ccatcagatg tttccagggt
      361 gccccaagga cctgaaatga ccctgtgcct tatttgaact aaccaatcag ttcgcttctc
      421 gcttctgttc gcgcgcttct gctccccgag ctcaataaaa gagcccacaa cccctcactc
      481 ggcgcgccag tcctccgata gactgcgtcg cccgggtacc cgtattccca ataaagcctc
      541 ttgctgtttg catccgaatc gtggactcgc tgatccttgg gagggtctcc tcagattgat
      601 tgactgccca cctcgggggt ctttcatttg gaggttccac cgagatttgg agacccctgc
      661 ccagggacca ccgacccccc cgccgggagg taagctggcc agcggtcgtt tcgtgtctgt
      721 ctctgtcttt gtgcgtgttt gtgccggcat ctaatgtttg cgcctgcgtc tgtactagtt
      781 agctaactag ctctgtatct ggcggacccg tggtggaact gacgagttct gaacacccgg
      841 ccgcaaccct gggagacgtc ccagggactt tgggggccgt ttttgtggcc cgacctgagg
      901 aagggagtcg atgtggaatc cgaccccgtc aggatatgtg gttctggtag gagacgagaa
      961 cctaaaacag ttcccgcctc cgtctgaatt tttgctttcg gtttggaacc gaagccgcgc
     1021 gtcttgtctg ctgcagcgct gcagcatcgt tctgtgttgt ctctgtctga ctgtgtttct
     1081 gtatttgtct gaaaattagg gccagactgt taccactccc ttaagtttga ccttaggtca
     1141 ctggaaagat gtcgagcgga tcgctcacaa ccagtcggta gatgtcaaga agagacgttg
     1201 ggttaccttc tgctctgcag aatggccaac ctttaacgtc ggatggccgc gagacggcac
     1261 ctttaaccga gacctcatca cccaggttaa gatcaaggtc ttttcacctg gcccgcatgg
     1321 acacccagac caggtcccct acatcgtgac ctgggaagcc ttggcttttg acccccctcc
     1381 ctgggtcaag ccctttgtac accctaagcc tccgcctcct cttcctccat ccgccccgtc
     1441 tctccccctt gaacctcctc gttcgacccc gcctcgatcc tccctttatc cagccctcac
     1501 tccttctcta ggcgccggaa ttagatctct cgagcaccat ggctagcatg actggtggac
     1561 agcaaatggg tggatccact agtaacggcc gccagtgtgc tggaattctt atgaggctgc
     1621 gggtgcggct tctgaagcgg acctggccgc tggaggtgcc cgagacggag ccgacgctgg
     1681 ggcatttgcg ctcgcacctg aggcagtccc tgctgtgcac ctgggggtac agttctaata
     1741 cccgatttac aattacattg aactacaagg atcccctcac tggagatgaa gagaccttgg
     1801 cttcatatgg gattgtttct ggggacttga tatgtttgat tcttcaagat gacattccag
     1861 cgcctaatat accttcatcc acagattcag agcattcttc actccagaat aatgagcaac
     1921 cctctttggc caccagctcc aatcagacta gcatgcagga tgaacaacca agtgattcat
     1981 tccaaggaca ggcagcccag tctggtgttt ggaatgacga cagtatgtta gggcctagtc
     2041 aaaattttga agctgagtca attcaagata atgcgcatat ggcagagggc acaggtttct
     2101 atccctcaga acccatgctc tgtagtgaat cggtggaagg gcaagtgcca cattcattag
     2161 agaccttgta tcaatcagct gactgttctg atgccaatga tgccttgata gtgttgatac
     2221 atcttctcat gttggagtca ggttacatac ctcagggcac cgaagccaaa gcactgtcca
     2281 tgccggagaa gtggaagttg agcggggtgt ataagctgca gtacatgcat cctctctgcg
     2341 agggcagctc cgctactctc acctgtgtgc ctttgggaaa cctgattgtt gtaaatgcta
     2401 cactaaaaat caacaatgag attagaagtg tgaaaagatt gcagctgcta ccagaatctt
     2461 ttatttgcaa agagaaacta ggggaaaatg tagccaacat atacaaagat cttcagaaac
     2521 tctctcgcct ctttaaagac cagctggtgt atcctcttct ggcttttacc cgacaagcac
     2581 tgaacctacc agatgtattt gggttggtcg tcctcccatt ggaactgaaa ctacggatct
     2641 tccgacttct ggatgttcgt tccgtcttgt ctttgtctgc ggtttgtcgt gacctcttta
     2701 ctgcttcaaa tgacccactc ctgtggaggt ttttatatct gcgtgatttt cgagacaata
     2761 ctgtcagagt tcaagacaca gattggaaag aactgtacag gaagaggcac atacaaagaa
     2821 aagaatcccc gaaagggcgg tttgtgatgc tcctgccatc gtcaactcac accattccat
     2881 tctatcccaa ccccttgcac cctaggccat ttcctagctc ccgccttcct ccaggaatta
     2941 tcgggggtga atatgaccaa agaccaacac ttccctatgt tggagaccca atcagttcac
     3001 tcattcctgg tcctggggag acgcccagcc agtttcctcc actgagacca cgctttgatc
     3061 cagttggccc acttccagga cctaacccca tcttgccagg gcgaggcggc cccaatgaca
     3121 gatttccctt tagacccagc aggggtcggc caactgatgg ccggctgtca ttcatgtgag
     3181 aattccgccc ccccccccta acgttactgg ccgaagccgc ttggaataag gccggtgtgc
     3241 gtttgtctat atgttatttt ccaccatatt gccgtctttt ggcaatgtga gggcccggaa
     3301 acctggccct gtcttcttga cgagcattcc taggggtctt tcccctctcg ccaaaggaat
     3361 gcaaggtctg ttgaatgtcg tgaaggaagc agttcctctg gaagcttctt gaagacaaac
     3421 aacgtctgta gcgacccttt gcaggcagcg gaacccccca cctggcgaca ggtgcctctg
     3481 cggccaaaag ccacgtgtat aagatacacc tgcaaaggcg gcacaacccc agtgccacgt
     3541 tgtgagttgg atagttgtgg aaagagtcaa atggctctcc tcaagcgtat tcaacaaggg
     3601 gctgaaggat gcccagaagg taccccattg tatgggatct gatctggggc ctcggtgcac
     3661 atgctttaca tgtgtttagt cgaggttaaa aaaacgtcta ggccccccga accacgggga
     3721 cgtggttttc ctttgaaaaa cacgatgata atatggccac aaccatggtg agcaagggcg
     3781 aggagctgtt caccggggtg gtgcccatcc tggtcgagct ggacggcgac gtaaacggcc
     3841 acaagttcag cgtgtctggc gagggcgagg gcgatgccac ctacggcaag ctgaccctga
     3901 agttcatctg caccaccggc aagctgcccg tgccctggcc caccctcgtg accaccctga
     3961 cctacggcgt gcagtgcttc agccgctacc ccgaccacat gaagcagcac gacttcttca
     4021 agtccgccat gcccgaaggc tacgtccagg agcgcaccat cttcttcaag gacgacggca
     4081 actacaagac ccgcgccgag gtgaagttcg agggcgacac cctggtgaac cgcatcgagc
     4141 tgaagggcat cgacttcaag gaggacggca acatcctggg gcacaagctg gagtacaact
     4201 acaacagcca caacgtctat atcatggccg acaagcagaa gaacggcatc aaggcgaact
     4261 tcaagatccg ccacaacatc gaggacggca gcgtgcagct cgccgaccac taccagcaga
     4321 acacccccat cggcgacggc cccgtgctgc tgcccgacaa ccactacctg agcacccagt
     4381 ccgccctgag caaagacccc aacgagaagc gcgatcacat ggtcctgctg gagttcgtga
     4441 ccgccgccgg gatcactctc ggcatggacg agctgtacaa gtaatgaatt aattaagaat
     4501 tatcaagctt atcgataccg tcgacctgca gccaagctta tcgataaaat aaaagatttt
     4561 atttagtctc cagaaaaagg ggggaatgaa agaccccacc tgtaggtttg gcaagctagc
     4621 ttaagtaacg ccattttgca aggcatggaa aatacataac tgagaataga gaagttcaga
     4681 tcaaggttag gaacagagag acagcagaat atgggccaaa caggatatct gtggtaagca
     4741 gttcctgccc cggctcaggg ccaagaacag atggtcccca gatgcggtcc cgccctcagc
     4801 agtttctaga gaaccatcag atgtttccag ggtgccccaa ggacctgaaa tgaccctgtg
     4861 ccttatttga actaaccaat cagttcgctt ctcgcttctg ttcgcgcgct tctgctcccc
     4921 gagctcaata aaagagccca caacccctca ctcggcgcgc cagtcctccg atagactgcg
     4981 tcgcccgggt acccgtgtat ccaataaacc ctcttgcagt tgcatccgac ttgtggtctc
     5041 gctgttcctt gggagggtct cctctgagtg attgactacc cgtcagcggg ggtctttcat
     5101 gggtaacagt ttcttgaagt tggagaacaa cattctgagg gtaggagtcg aatattaagt
     5161 aatcctgact caattagcca ctgttttgaa tccacatact ccaatactcc tgaaatagtt
     5221 cattatggac agcgcagaaa gagctgggga gaattgtgaa attgttatcc gctcacaatt
     5281 ccacacaaca tacgagccgg aagcataaag tgtaaagcct ggggtgccta atgagtgagc
     5341 taactcacat taattgcgtt gcgctcactg cccgctttcc agtcgggaaa cctgtcgtgc
     5401 cagctgcatt aatgaatcgg ccaacgcgcg gggagaggcg gtttgcgtat tgggcgctct
     5461 tccgcttcct cgctcactga ctcgctgcgc tcggtcgttc ggctgcggcg agcggtatca
     5521 gctcactcaa aggcggtaat acggttatcc acagaatcag gggataacgc aggaaagaac
     5581 atgtgagcaa aaggccagca aaaggccagg aaccgtaaaa aggccgcgtt gctggcgttt
     5641 ttccataggc tccgcccccc tgacgagcat cacaaaaatc gacgctcaag tcagaggtgg
     5701 cgaaacccga caggactata aagataccag gcgtttcccc ctggaagctc cctcgtgcgc
     5761 tctcctgttc cgaccctgcc gcttaccgga tacctgtccg cctttctccc ttcgggaagc
     5821 gtggcgcttt ctcatagctc acgctgtagg tatctcagtt cggtgtaggt cgttcgctcc
     5881 aagctgggct gtgtgcacga accccccgtt cagcccgacc gctgcgcctt atccggtaac
     5941 tatcgtcttg agtccaaccc ggtaagacac gacttatcgc cactggcagc agccactggt
     6001 aacaggatta gcagagcgag gtatgtaggc ggtgctacag agttcttgaa gtggtggcct
     6061 aactacggct acactagaag aacagtattt ggtatctgcg ctctgctgaa gccagttacc
     6121 ttcggaaaaa gagttggtag ctcttgatcc ggcaaacaaa ccaccgctgg tagcggtggt
     6181 ttttttgttt gcaagcagca gattacgcgc agaaaaaaag gatctcaaga agatcctttg
     6241 atcttttcta cggggtctga cgctcagtgg aacgaaaact cacgttaagg gattttggtc
     6301 atgagattat caaaaaggat cttcacctag atccttttaa attaaaaatg aagttttaaa
     6361 tcaatctaaa gtatatatga gtaaacttgg tctgacagtt accaatgctt aatcagtgag
     6421 gcacctatct cagcgatctg tctatttcgt tcatccatag ttgcctgact ccccgtcgtg
     6481 tagataacta cgatacggga gggcttacca tctggcccca gtgctgcaat gataccgcga
     6541 gacccacgct caccggctcc agatttatca gcaataaacc agccagccgg aagggccgag
     6601 cgcagaagtg gtcctgcaac tttatccgcc tccatccagt ctattaattg ttgccgggaa
     6661 gctagagtaa gtagttcgcc agttaatagt ttgcgcaacg ttgttgccat tgctacaggc
     6721 atcgtggtgt cacgctcgtc gtttggtatg gcttcattca gctccggttc ccaacgatca
     6781 aggcgagtta catgatcccc catgttgtgc aaaaaagcgg ttagctcctt cggtcctccg
     6841 atcgttgtca gaagtaagtt ggccgcagtg ttatcactca tggttatggc agcactgcat
     6901 aattctctta ctgtcatgcc atccgtaaga tgcttttctg tgactggtga gtactcaacc
     6961 aagtcattct gagaatagtg tatgcggcga ccgagttgct cttgcccggc gtcaatacgg
     7021 gataataccg cgccacatag cagaacttta aaagtgctca tcattggaaa acgttcttcg
     7081 gggcgaaaac tctcaaggat cttaccgctg ttgagatcca gttcgatgta acccactcgt
     7141 gcacccaact gatcttcagc atcttttact ttcaccagcg tttctgggtg agcaaaaaca
     7201 ggaaggcaaa atgccgcaaa aaagggaata agggcgacac ggaaatgttg aatactcata
     7261 ctcttccttt ttcaatatta ttgaagcatt tatcagggtt attgtctcat gagcggatac
     7321 atatttgaat gtatttagaa aaataaacaa ataggggttc cgcgcacatt tccccgaaaa
     7381 gtgccacctg acgtctaaga aaccattatt atcatgacat taacctataa aaataggcgt
     7441 atcacgaggc cctttcgtct cgcgcgtttc ggtgatgacg gtgaaaacct ctgacacatg
     7501 cagctcccgg agacggtcac agcttgtctg taagcggatg ccgggagcag acaagcccgt
     7561 cagggcgcgt cagcgggtgt tggcgggtgt cggggctggc ttaactatgc ggcatcagag
     7621 cagattgtac tgagagtgca ccatatgcgg tgtgaaatac cgcacagatg cgtaaggaga
     7681 aaataccgca tcaggcgcca ttcgccattc aggctgcgca actgttggga agggcgatcg
     7741 gtgcgggcct cttcgctatt acgccagctg gcgaaagggg gatgtgctgc aaggcgatta
     7801 agttgggtaa cgccagggtt ttcccagtca cgacgttgta aaacgacggc gcaaggaatg
     7861 gtgcatgcaa ggagatggcg cccaacagtc ccccggccac ggggcctgcc accataccca
     7921 cgccgaaaca agcgctcatg agcccgaagt ggcgagcccg atcttcccca tcggtgatgt
     7981 cggcgatata ggcgccagca accgcacctg tggcgccggt gatgccggcc acgatgcgtc
     8041 cggcgtagag gcgattagtc caatttgtta aagacaggat
//
