LOCUS       sequence-35337-e         809 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 809)
  AUTHORS   Hussein R, Lim HN
  TITLE     Disruption of small RNA signaling caused by competition for Hfq.
  JOURNAL   Proc Natl Acad Sci U S A. 2011 Jan 18;108(3):1110-5. doi: 
            10.1073/pnas.1010082108. Epub 2010 Dec 28.
  PUBMED    21189298
REFERENCE   2  (bases 1 to 809)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1073/pnas.1010082108"; journalName: "Proc Natl Acad Sci U S A"; 
            date: "2011-01-18- 18"; volume: "108"; issue: "3"; pages: "1110-5"
COMMENT     Sequence Label: HL 4180 (=HL 2752 + pHL 1358)
FEATURES             Location/Qualifiers
     source          1..809
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    222..240
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    247..265
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     primer_bind     complement(286..304)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        372..476
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(695..714)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
ORIGIN
        1 cctttacgca tgctcgcggt acctttaatc aggttgttca ggttagtgac ataagtctga
       61 tggtgcttgc cgtagtgata ctcgatggtt tccgcagaaa tgtgcggtgc cagagcatct
      121 ttagcatatg gtagtgcagg taattcgaat gacattgcta ctctccttta ttattaattt
      181 gcatacgtac aatagcctta ttgtgcgtat ggtcgacagt ctctctatca ctgataggga
      241 tgtcaatctc tatcactgat agggactcga ggtgaagacg aaagggcctc gtgatacgcc
      301 tatttttata ggttaatgtc atgataataa tggtttctta gacgtcaggt ggcacttttc
      361 ggggaaatgt gcgcggaacc cctatttgtt tatttttcta aatacattca aatatgtatc
      421 cgctcatgag acaataaccc tgataaatgc ttcaataata ttgaaaaagg aagagtatga
      481 gtattcaaca tttccgtgtc gcccttattc ccttttttgc ggcattttgc cttcctgttt
      541 ttgctcaccc agaaacgctg gtgaaagtaa aagatgctga agatcagttg ggtgcacgag
      601 tgggttacat cgaactggat ctcaacagcg gtaagatcct tgagagtttt cgccccgaag
      661 aacgttttcc aatgatgagc acttttaaag ttctgctatg tggcgcggta ttatcccgta
      721 ttgacgccgg gcaagagcaa ctcggtcgcc gcatacacta ttctcagaat gacttgnttg
      781 agtactcacc agtcacagaa aagcatctt
//
