LOCUS       sequence-353391-       11491 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11491)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 11491)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF3342
FEATURES             Location/Qualifiers
     source          1..11491
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        1..1179
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          232..1170
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     1127..1147
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     protein_bind    1199..1223
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(4245..4269)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             4277..4318
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     misc_feature    4429..5017
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(4482..4502)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(4900..4911)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             5089..5322
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    5400..5521
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(5437..5456)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     5491..5510
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      5561..5696
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(5716..5735)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(5717..5735)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(5745..5762)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(5745..5761)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(5754..5776)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      5903..6358
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(5990..6009)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     6200..6221
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        6384..6488
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(6489..6557,6558..7349)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(6707..6726)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      7520..8108
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     8009..8028
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     8262..8279
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    8396..8417
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(8432..8437,8438..8455,8456..8462)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    8470..8486
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     8475..8497
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     8494..8510
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     8494..8510
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     8529..8549
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        8531..8549
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        8577..8803
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             8804..8984
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    9031..9156
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    9649..9882
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             10067..10111
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     promoter        10342..10552
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     promoter        10364..10560
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     rep_origin      10411..10546
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             10592..11194
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGAS"
     primer_bind     complement(10592..10611)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     11088..11108
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     misc_feature    11237..11354
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
ORIGIN
        1 ggctccggtg cccgtcagtg ggcagagcgc acatcgccca cagtccccga gaagttgggg
       61 ggaggggtcg gcaattgaac cggtgcctag agaaggtggc gcggggtaaa ctgggaaagt
      121 gatgtcgtgt actggctccg cctttttccc gagggtgggg gagaaccgta tataagtgca
      181 gtagtcgccg tgaacgttct ttttcgcaac gggtttgccg ccagaacaca ggtaagtgcc
      241 gtgtgtggtt cccgcgggcc tggcctcttt acgggttatg gcccttgcgt gccttgaatt
      301 acttccacct ggctgcagta cgtgattctt gatcccgagc ttcgggttgg aagtgggtgg
      361 gagagttcga ggccttgcgc ttaaggagcc ccttcgcctc gtgcttgagt tgaggcctgg
      421 cctgggcgct ggggccgccg cgtgcgaatc tggtggcacc ttcgcgcctg tctcgctgct
      481 ttcgataagt ctctagccat ttaaaatttt tgatgacctg ctgcgacgct ttttttctgg
      541 caagatagtc ttgtaaatgc gggccaagat ctgcacactg gtatttcggt ttttggggcc
      601 gcgggcggcg acggggcccg tgcgtcccag cgcacatgtt cggcgaggcg gggcctgcga
      661 gcgcggccac cgagaatcgg acgggggtag tctcaagctg gccggcctgc tctggtgcct
      721 ggcctcgcgc cgccgtgtat cgccccgccc tgggcggcaa ggctggcccg gtcggcacca
      781 gttgcgtgag cggaaagatg gccgcttccc ggccctgctg cagggagctc aaaatggagg
      841 acgcggcgct cgggagagcg ggcgggtgag tcacccacac aaaggaaaag ggcctttccg
      901 tcctcagccg tcgcttcatg tgactccacg gagtaccggg cgccgtccag gcacctcgat
      961 tagttctcga gcttttggag tacgtcgtct ttaggttggg gggaggggtt ttatgcgatg
     1021 gagtttcccc acactgagtg ggtggagact gaagttaggc cagcttggca cttgatgtaa
     1081 ttctccttgg aatttgccct ttttgagttt ggatcttggt tcattctcaa gcctcagaca
     1141 gtggttcaaa gtttttttct tccatttcag gtgtcgtgag gctagcatcg attgatcaac
     1201 aagtttgtac aaaaaagcag gcaccatgga tctgactctt gctgaacttc aggaaatggc
     1261 atctcgccag cagcaacaga ttgaagccca gcaacaattg ctggcaacta aggaacagcg
     1321 cttaaagttt ttgaaacaac aagatcagcg acaacagcaa caagttgctg agcaggagaa
     1381 acttaaaagg ctaaaagaaa tagctgagaa tcaggaagct aagctaaaaa aagtgagagc
     1441 acttaaaggc cacgtggaac agaagagact aagcaatggg aaacttgtgg aggaaattga
     1501 acagatgaat aatttgttcc agcaaaaaca gagggagctc gtcctggctg tgtcaaaagt
     1561 agaagaactg accaggcagc tagagatgct caagaacggc aggatcgaca gccaccatga
     1621 caatcagtct gcagtggctg agcttgatcg cctctataag gagctgcagc taagaaacaa
     1681 attgaatcaa gagcagaatg ccaagctaca acaacagagg gagtgtttga ataagcgtaa
     1741 ttcagaagtg gcagtcatgg ataagcgtgt taatgagctg agggaccggc tgtggaagaa
     1801 gaaggcagct ctacagcaaa aagaaaatct accagtttca tctgatggaa atcttcccca
     1861 gcaagccgcg tcagccccaa gccgtgtggc tgcagtaggt ccctatatcc agtcgtctac
     1921 tatgcctcgg atgccctcaa ggcctgaatt gctggtgaag ccagccctgc cggatggttc
     1981 cttggtcatt caggcttcag aggggccgat gaaaatacag acactgccca acatgagatc
     2041 tggggctgct tcacaaacta aaggctctaa aatccatcca gttggccctg attggagtcc
     2101 ttcaaatgca gatcttttcc caagccaagg ctctgcttct gtacctcaaa gcactgggaa
     2161 tgctctggat caagttgatg atggagaggt tccgctgagg gagaaagaga agaaagtgcg
     2221 tccgttctca atgtttgatg cagtagacca gtccaatgcc ccaccttcct ttggtactct
     2281 gaggaagaac cagagcagtg aagatatctt gcgggatgct caggttgcaa ataaaaatgt
     2341 ggctaaagta ccacctcctg ttcctacaaa accaaaacag attaatttgc cttattttgg
     2401 acaaactaat cagccacctt cagacattaa gccagacgga agttctcagc agttgtcaac
     2461 agttgttccg tccatgggaa ctaaaccaaa accagcaggg cagcagccga gagtgctgct
     2521 atctcccagc ataccttcgg ttggccaaga ccagaccctt tctccaggtt ctaagcaaga
     2581 aagtccacct gctgctgccg tccggccctt tactccccag ccttccaaag acaccttact
     2641 tccacccttc agaaaacccc agaccgtggc agcaagttca atatattcca tgtatacgca
     2701 acagcaggcg ccaggaaaaa acttccagca ggctgtgcag agcgcgttga ccaagactca
     2761 taccagaggg ccacactttt caagtgtata tggtaagcct gtaattgctg ctgcccagaa
     2821 tcaacagcag cacccagaga acatttattc caatagccag ggcaagcctg gcagtccaga
     2881 acctgaaaca gagcctgttt cttcagttca ggagaaccat gaaaacgaaa gaattcctcg
     2941 gccactcagc ccaactaaat tactgccttt cttatctaat ccttaccgaa accagagtga
     3001 tgctgaccta gaagccttac gaaagaaact gtctaacgca ccaaggcctc taaagaaacg
     3061 tagttctatt acagagccag agggtcctaa tgggccaaat attcagaagc ttttatatca
     3121 gaggaccacc atagcggcca tggagaccat ctctgtccca tcatacccat ccaagtcagc
     3181 ttctgtgact gccagctcag aaagcccagt agaaatccag aatccatatt tacatgtgga
     3241 gcccgaaaag gaggtggtct ctctggttcc tgaatcattg tccccagagg atgtggggaa
     3301 tgccagtaca gagaacagtg acatgccagc tccttctcca ggccttgatt atgagcctga
     3361 gggagtccca gacaacagcc caaatctcca gaataaccca gaagaaccaa atccagaggc
     3421 tccacatgtg cttgatgtgt acctggagga gtaccctcca tacccacccc caccataccc
     3481 atctggggag cctgaagggc ccggagaaga ctcggtgagc atgcgcccgc ctgaaatcac
     3541 cgggcaggtc tctctgcctc ctggtaaaag gacaaacttg cgtaaaactg gctcagagcg
     3601 tatcgctcat ggaatgaggg tgaaattcaa cccccttgct ttactgctag attcgtcttt
     3661 ggagggagaa tttgaccttg tacagagaat tatttatgag gttgatgacc caagcctccc
     3721 caatgatgaa ggcatcacgg ctcttcacaa tgctgtgtgt gcaggccaca cagaaatcgt
     3781 taagttcctg gtacagtttg gtgtaaatgt aaatgctgct gatagtgatg gatggactcc
     3841 attacattgt gctgcctcat gtaacaacgt ccaagtgtgt aagtttttgg tggagtcagg
     3901 agccgctgtg tttgccatga cctacagtga catgcagact gctgcagata agtgcgagga
     3961 aatggaggaa ggctacactc agtgctccca atttctttat ggagttcagg agaagatggg
     4021 cataatgaat aaaggagtca tttatgcgct ttgggattat gaacctcaga atgatgatga
     4081 gctgcccatg aaagaaggag actgcatgac aatcatccac agggaagacg aagatgaaat
     4141 cgaatggtgg tgggcgcgcc ttaatgataa ggagggatat gttccacgta acttgctggg
     4201 actgtaccca agaattaaac caagacaaag gagcttggcc tgagacccag ctttcttgta
     4261 caaagtggtt gatatcggta agcctatccc taaccctctc ctcggtctcg attctacgta
     4321 gtaatgaact agtccgtaac ttgaaagtat ttcgatttct tggctttata tatcttgtgg
     4381 aaaggacgac ccgcacgcaa actacattcg tcaacgcgtt aagtcgacaa tcaacctctg
     4441 gattacaaaa tttgtgaaag attgactggt attcttaact atgttgctcc ttttacgcta
     4501 tgtggatacg ctgctttaat gcctttgtat catgctattg cttcccgtat ggctttcatt
     4561 ttctcctcct tgtataaatc ctggttgctg tctctttatg aggagttgtg gcccgttgtc
     4621 aggcaacgtg gcgtggtgtg cactgtgttt gctgacgcaa cccccactgg ttggggcatt
     4681 gccaccacct gtcagctcct ttccgggact ttcgctttcc ccctccctat tgccacggcg
     4741 gaactcatcg ccgcctgcct tgcccgctgc tggacagggg ctcggctgtt gggcactgac
     4801 aattccgtgg tgttgtcggg gaaatcatcg tcctttcctt ggctgctcgc ctgtgttgcc
     4861 acctggattc tgcgcgggac gtccttctgc tacgtccctt cggccctcaa tccagcggac
     4921 cttccttccc gcggcctgct gccggctctg cggcctcttc cgcgtcttcg ccttcgccct
     4981 cagacgagtc ggatctccct ttgggccgcc tccccgcgtc gactttaaga ccaatgactt
     5041 acaaggcagc tgtagatctt agccactttt taaaagaaaa ggggggactg gaagggctaa
     5101 ttcactccca acgaagacaa gatctgcttt ttgcttgtac tgggtctctc tggttagacc
     5161 agatctgagc ctgggagctc tctggctaac tagggaaccc actgcttaag cctcaataaa
     5221 gcttgccttg agtgcttcaa gtagtgtgtg cccgtctgtt gtgtgactct ggtaactaga
     5281 gatccctcag acccttttag tcagtgtgga aaatctctag cagtacgtat agtagttcat
     5341 gtcatcttat tattcagtat ttataacttg caaagaaatg aatatcagag agtgagagga
     5401 acttgtttat tgcagcttat aatggttaca aataaagcaa tagcatcaca aatttcacaa
     5461 ataaagcatt tttttcactg cattctagtt gtggtttgtc caaactcatc aatgtatctt
     5521 atcatgtctg gctctagcta tcccgcccct aactccgccc atcccgcccc taactccgcc
     5581 cagttccgcc cattctccgc cccatggctg actaattttt tttatttatg cagaggccga
     5641 ggccgcctcg gcctctgagc tattccagaa gtagtgagga ggcttttttg gaggcctagg
     5701 gacgtaccca attcgcccta tagtgagtcg tattacgcgc gctcactggc cgtcgtttta
     5761 caacgtcgtg actgggaaaa ccctggcgtt acccaactta atcgccttgc agcacatccc
     5821 cctttcgcca gctggcgtaa tagcgaagag gcccgcaccg atcgcccttc ccaacagttg
     5881 cgcagcctga atggcgaatg ggacgcgccc tgtagcggcg cattaagcgc ggcgggtgtg
     5941 gtggttacgc gcagcgtgac cgctacactt gccagcgccc tagcgcccgc tcctttcgct
     6001 ttcttccctt cctttctcgc cacgttcgcc ggctttcccc gtcaagctct aaatcggggg
     6061 ctccctttag ggttccgatt tagtgcttta cggcacctcg accccaaaaa acttgattag
     6121 ggtgatggtt cacgtagtgg gccatcgccc tgatagacgg tttttcgccc tttgacgttg
     6181 gagtccacgt tctttaatag tggactcttg ttccaaactg gaacaacact caaccctatc
     6241 tcggtctatt cttttgattt ataagggatt ttgccgattt cggcctattg gttaaaaaat
     6301 gagctgattt aacaaaaatt taacgcgaat tttaacaaaa tattaacgct tacaatttag
     6361 gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt
     6421 caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa
     6481 ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt
     6541 gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt
     6601 tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt
     6661 ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg
     6721 tattatcccg tattgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga
     6781 atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa
     6841 gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga
     6901 caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa
     6961 ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca
     7021 ccacgatgcc tgtagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta
     7081 ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac
     7141 ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc
     7201 gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag
     7261 ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga
     7321 taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt
     7381 agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata
     7441 atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag
     7501 aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa
     7561 caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt
     7621 ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgttctt ctagtgtagc
     7681 cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa
     7741 tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa
     7801 gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc
     7861 ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa
     7921 gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa
     7981 caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg
     8041 ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc
     8101 tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg
     8161 ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg
     8221 agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg
     8281 aagcggaaga gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg attcattaat
     8341 gcagctggca cgacaggttt cccgactgga aagcgggcag tgagcgcaac gcaattaatg
     8401 tgagttagct cactcattag gcaccccagg ctttacactt tatgcttccg gctcgtatgt
     8461 tgtgtggaat tgtgagcgga taacaatttc acacaggaaa cagctatgac catgattacg
     8521 ccaagcgcgc aattaaccct cactaaaggg aacaaaagct ggagctgcaa gcttaatgta
     8581 gtcttatgca atactcttgt agtcttgcaa catggtaacg atgagttagc aacatgcctt
     8641 acaaggagag aaaaagcacc gtgcatgccg attggtggaa gtaaggtggt acgatcgtgc
     8701 cttattagga aggcaacaga cgggtctgac atggattgga cgaaccactg aattgccgca
     8761 ttgcagagat attgtattta agtgcctagc tcgatacata aacgggtctc tctggttaga
     8821 ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata
     8881 aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta
     8941 gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtggcg cccgaacagg
     9001 gacttgaaag cgaaagggaa accagaggag ctctctcgac gcaggactcg gcttgctgaa
     9061 gcgcgcacgg caagaggcga ggggcggcga ctggtgagta cgccaaaaat tttgactagc
     9121 ggaggctaga aggagagaga tgggtgcgag agcgtcagta ttaagcgggg gagaattaga
     9181 tcgcgatggg aaaaaattcg gttaaggcca gggggaaaga aaaaatataa attaaaacat
     9241 atagtatggg caagcaggga gctagaacga ttcgcagtta atcctggcct gttagaaaca
     9301 tcagaaggct gtagacaaat actgggacag ctacaaccat cccttcagac aggatcagaa
     9361 gaacttagat cattatataa tacagtagca accctctatt gtgtgcatca aaggatagag
     9421 ataaaagaca ccaaggaagc tttagacaag atagaggaag agcaaaacaa aagtaagacc
     9481 accgcacagc aagcggccgc tgatcttcag acctggagga ggagatatga gggacaattg
     9541 gagaagtgaa ttatataaat ataaagtagt aaaaattgaa ccattaggag tagcacccac
     9601 caaggcaaag agaagagtgg tgcagagaga aaaaagagca gtgggaatag gagctttgtt
     9661 ccttgggttc ttgggagcag caggaagcac tatgggcgca gcgtcaatga cgctgacggt
     9721 acaggccaga caattattgt ctggtatagt gcagcagcag aacaatttgc tgagggctat
     9781 tgaggcgcaa cagcatctgt tgcaactcac agtctggggc atcaagcagc tccaggcaag
     9841 aatcctggct gtggaaagat acctaaagga tcaacagctc ctggggattt ggggttgctc
     9901 tggaaaactc atttgcacca ctgctgtgcc ttggaatgct agttggagta ataaatctct
     9961 ggaacagatt tggaatcaca cgacctggat ggagtgggac agagaaatta acaattacac
    10021 aagcttaata cactccttaa ttgaagaatc gcaaaaccag caagaaaaga atgaacaaga
    10081 attattggaa ttagataaat gggcaagttt gtggaattgg tttaacataa caaattggct
    10141 gtggtatata aaattattca taatgatagt aggaggcttg gtaggtttaa gaatagtttt
    10201 tgctgtactt tctatagtga atagagttag gcagggatat tcaccattat cgtttcagac
    10261 ccacctccca accccgaggg gacccatgca ttgcatctca attagtcagc aaccaggtgt
    10321 ggaaagtccc caggctcccc agcaggcaga agtatgcaaa gcatgcgtct caattagtca
    10381 gcaaccatag tcccgcccct aactccgccc atcccgcccc taactccgcc cagttccgcc
    10441 cattctccgc cccatggctg actaattttt tttatttatg cagaggccga ggccgcctcg
    10501 gcctctgagc tattccagaa gtagtgagga ggcttttttg gaggcctagg cttttgcaaa
    10561 aagctttcta gaggtaccac cggagcttac catgaccgag tacaagccca cggtgcgcct
    10621 cgccacccgc gacgacgtcc ccagggccgt acgcaccctc gccgccgcgt tcgccgacta
    10681 ccccgccacg cgccacaccg tcgatccgga ccgccacatc gagcgggtca ccgagctgca
    10741 agaactcttc ctcacgcgcg tcgggctcga catcggcaag gtgtgggtcg cggacgacgg
    10801 cgccgccgtg gcggtctgga ccacgccgga gagcgtcgaa gcgggggcgg tgttcgccga
    10861 gatcggcccg cgcatggccg agttgagcgg ttcccggctg gccgcgcagc aacagatgga
    10921 aggcctcctg gcgccgcacc ggcccaagga gcccgcgtgg ttcctggcca ccgtcggcgt
    10981 ctcgcccgac caccagggca agggtctggg cagcgccgtc gtgctccccg gagtggaggc
    11041 ggccgagcgc gccggggtgc ccgccttcct ggagacctcc gcgccccgca acctcccctt
    11101 ctacgagcgg ctcggcttca ccgtcaccgc cgacgtcgag gtgcccgaag gaccgcgcac
    11161 ctggtgcatg acccgcaagc ccggtgccag ttaacgaatt ctagatcttg agacaaatgg
    11221 cagtattcat ccacaatttt aaaagaaaag gggggattgg ggggtacagt gcaggggaaa
    11281 gaatagtaga cataatagca acagacatac aaactaaaga attacaaaaa caaattacaa
    11341 aaattcaaaa ttttcgggtt tattacaggg acagcagaga tccactttgg cgccggctcg
    11401 aggcctgcag gtgcaaagat ggataaagtt ttaaacagag aggaatcttt gcagctaatg
    11461 gaccttctag gtcttgaaag gagtgggaat t
//
