LOCUS       sequence-353392-        9133 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9133)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 9133)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF3354
FEATURES             Location/Qualifiers
     source          1..9133
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        1..1179
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          232..1170
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     1127..1147
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     protein_bind    1199..1223
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(1887..1911)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             1919..1960
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     misc_feature    2071..2659
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(2124..2144)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(2542..2553)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             2731..2964
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    3042..3163
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(3079..3098)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     3133..3152
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      3203..3338
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(3358..3377)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(3359..3377)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(3387..3404)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(3387..3403)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(3396..3418)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      3545..4000
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(3632..3651)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     3842..3863
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        4026..4130
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(4131..4199,4200..4991)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(4349..4368)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      5162..5750
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     5651..5670
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     5904..5921
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    6038..6059
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(6074..6079,6080..6097,6098..6104)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6112..6128
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     6117..6139
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     6136..6152
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     6136..6152
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     6171..6191
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        6173..6191
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        6219..6445
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             6446..6626
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    6673..6798
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    7291..7524
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             7709..7753
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     promoter        7984..8194
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     promoter        8006..8202
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     rep_origin      8053..8188
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             8234..8836
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGAS"
     primer_bind     complement(8234..8253)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     8730..8750
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     misc_feature    8879..8996
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
ORIGIN
        1 ggctccggtg cccgtcagtg ggcagagcgc acatcgccca cagtccccga gaagttgggg
       61 ggaggggtcg gcaattgaac cggtgcctag agaaggtggc gcggggtaaa ctgggaaagt
      121 gatgtcgtgt actggctccg cctttttccc gagggtgggg gagaaccgta tataagtgca
      181 gtagtcgccg tgaacgttct ttttcgcaac gggtttgccg ccagaacaca ggtaagtgcc
      241 gtgtgtggtt cccgcgggcc tggcctcttt acgggttatg gcccttgcgt gccttgaatt
      301 acttccacct ggctgcagta cgtgattctt gatcccgagc ttcgggttgg aagtgggtgg
      361 gagagttcga ggccttgcgc ttaaggagcc ccttcgcctc gtgcttgagt tgaggcctgg
      421 cctgggcgct ggggccgccg cgtgcgaatc tggtggcacc ttcgcgcctg tctcgctgct
      481 ttcgataagt ctctagccat ttaaaatttt tgatgacctg ctgcgacgct ttttttctgg
      541 caagatagtc ttgtaaatgc gggccaagat ctgcacactg gtatttcggt ttttggggcc
      601 gcgggcggcg acggggcccg tgcgtcccag cgcacatgtt cggcgaggcg gggcctgcga
      661 gcgcggccac cgagaatcgg acgggggtag tctcaagctg gccggcctgc tctggtgcct
      721 ggcctcgcgc cgccgtgtat cgccccgccc tgggcggcaa ggctggcccg gtcggcacca
      781 gttgcgtgag cggaaagatg gccgcttccc ggccctgctg cagggagctc aaaatggagg
      841 acgcggcgct cgggagagcg ggcgggtgag tcacccacac aaaggaaaag ggcctttccg
      901 tcctcagccg tcgcttcatg tgactccacg gagtaccggg cgccgtccag gcacctcgat
      961 tagttctcga gcttttggag tacgtcgtct ttaggttggg gggaggggtt ttatgcgatg
     1021 gagtttcccc acactgagtg ggtggagact gaagttaggc cagcttggca cttgatgtaa
     1081 ttctccttgg aatttgccct ttttgagttt ggatcttggt tcattctcaa gcctcagaca
     1141 gtggttcaaa gtttttttct tccatttcag gtgtcgtgag gctagcatcg attgatcaac
     1201 aagtttgtac aaaaaagttg gcatggcggc ggcggttcgg atgaacatcc agatgctgct
     1261 ggaggcggcc gactatctgg agcggcggga gagagaagct gaacatggtt atgcctccat
     1321 gttaccatac aataacaagg acagagatgc cttaaaacgg aggaacaaat ccaaaaagaa
     1381 taacagcagt agcagatcaa ctcacaatga aatggagaag aatagacggg ctcatcttcg
     1441 cttgtgcctg gagaagttga aggggctggt gccactggga cccgaatcaa gtcgacacac
     1501 tacgttgagt ttattaacaa aagccaaatt gcacataaag aaacttgaag attgtgacag
     1561 aaaagccgtt caccaaatcg accagcttca gcgagagcag cgacacctga agaggcagct
     1621 ggagaagctg ggcattgaga ggatccggat ggacagcatc ggctccaccg tctcctcgga
     1681 gcgctccgac tccgacaggg aagaaatcga cgttgacgtg gagagcacgg actatctcac
     1741 aggtgatctg gactggagca gcagcagtgt gagcgactct gacgagcggg gcagcatgca
     1801 gagcctcggc agtgatgagg gctattccag caccagcatc aagagaataa agctgcagga
     1861 cagtcacaag gcgtgtcttg gtctctaccc aactttcttg tacaaagtgg ttgatatcgg
     1921 taagcctatc cctaaccctc tcctcggtct cgattctacg tagtaatgaa ctagtccgta
     1981 acttgaaagt atttcgattt cttggcttta tatatcttgt ggaaaggacg atttacacct
     2041 ccgttaacca accccacgcg ttaagtcgac aatcaacctc tggattacaa aatttgtgaa
     2101 agattgactg gtattcttaa ctatgttgct ccttttacgc tatgtggata cgctgcttta
     2161 atgcctttgt atcatgctat tgcttcccgt atggctttca ttttctcctc cttgtataaa
     2221 tcctggttgc tgtctcttta tgaggagttg tggcccgttg tcaggcaacg tggcgtggtg
     2281 tgcactgtgt ttgctgacgc aacccccact ggttggggca ttgccaccac ctgtcagctc
     2341 ctttccggga ctttcgcttt ccccctccct attgccacgg cggaactcat cgccgcctgc
     2401 cttgcccgct gctggacagg ggctcggctg ttgggcactg acaattccgt ggtgttgtcg
     2461 gggaaatcat cgtcctttcc ttggctgctc gcctgtgttg ccacctggat tctgcgcggg
     2521 acgtccttct gctacgtccc ttcggccctc aatccagcgg accttccttc ccgcggcctg
     2581 ctgccggctc tgcggcctct tccgcgtctt cgccttcgcc ctcagacgag tcggatctcc
     2641 ctttgggccg cctccccgcg tcgactttaa gaccaatgac ttacaaggca gctgtagatc
     2701 ttagccactt tttaaaagaa aaggggggac tggaagggct aattcactcc caacgaagac
     2761 aagatctgct ttttgcttgt actgggtctc tctggttaga ccagatctga gcctgggagc
     2821 tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct tgagtgcttc
     2881 aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc agaccctttt
     2941 agtcagtgtg gaaaatctct agcagtacgt atagtagttc atgtcatctt attattcagt
     3001 atttataact tgcaaagaaa tgaatatcag agagtgagag gaacttgttt attgcagctt
     3061 ataatggtta caaataaagc aatagcatca caaatttcac aaataaagca tttttttcac
     3121 tgcattctag ttgtggtttg tccaaactca tcaatgtatc ttatcatgtc tggctctagc
     3181 tatcccgccc ctaactccgc ccatcccgcc cctaactccg cccagttccg cccattctcc
     3241 gccccatggc tgactaattt tttttattta tgcagaggcc gaggccgcct cggcctctga
     3301 gctattccag aagtagtgag gaggcttttt tggaggccta gggacgtacc caattcgccc
     3361 tatagtgagt cgtattacgc gcgctcactg gccgtcgttt tacaacgtcg tgactgggaa
     3421 aaccctggcg ttacccaact taatcgcctt gcagcacatc cccctttcgc cagctggcgt
     3481 aatagcgaag aggcccgcac cgatcgccct tcccaacagt tgcgcagcct gaatggcgaa
     3541 tgggacgcgc cctgtagcgg cgcattaagc gcggcgggtg tggtggttac gcgcagcgtg
     3601 accgctacac ttgccagcgc cctagcgccc gctcctttcg ctttcttccc ttcctttctc
     3661 gccacgttcg ccggctttcc ccgtcaagct ctaaatcggg ggctcccttt agggttccga
     3721 tttagtgctt tacggcacct cgaccccaaa aaacttgatt agggtgatgg ttcacgtagt
     3781 gggccatcgc cctgatagac ggtttttcgc cctttgacgt tggagtccac gttctttaat
     3841 agtggactct tgttccaaac tggaacaaca ctcaacccta tctcggtcta ttcttttgat
     3901 ttataaggga ttttgccgat ttcggcctat tggttaaaaa atgagctgat ttaacaaaaa
     3961 tttaacgcga attttaacaa aatattaacg cttacaattt aggtggcact tttcggggaa
     4021 atgtgcgcgg aacccctatt tgtttatttt tctaaataca ttcaaatatg tatccgctca
     4081 tgagacaata accctgataa atgcttcaat aatattgaaa aaggaagagt atgagtattc
     4141 aacatttccg tgtcgccctt attccctttt ttgcggcatt ttgccttcct gtttttgctc
     4201 acccagaaac gctggtgaaa gtaaaagatg ctgaagatca gttgggtgca cgagtgggtt
     4261 acatcgaact ggatctcaac agcggtaaga tccttgagag ttttcgcccc gaagaacgtt
     4321 ttccaatgat gagcactttt aaagttctgc tatgtggcgc ggtattatcc cgtattgacg
     4381 ccgggcaaga gcaactcggt cgccgcatac actattctca gaatgacttg gttgagtact
     4441 caccagtcac agaaaagcat cttacggatg gcatgacagt aagagaatta tgcagtgctg
     4501 ccataaccat gagtgataac actgcggcca acttacttct gacaacgatc ggaggaccga
     4561 aggagctaac cgcttttttg cacaacatgg gggatcatgt aactcgcctt gatcgttggg
     4621 aaccggagct gaatgaagcc ataccaaacg acgagcgtga caccacgatg cctgtagcaa
     4681 tggcaacaac gttgcgcaaa ctattaactg gcgaactact tactctagct tcccggcaac
     4741 aattaataga ctggatggag gcggataaag ttgcaggacc acttctgcgc tcggcccttc
     4801 cggctggctg gtttattgct gataaatctg gagccggtga gcgtgggtct cgcggtatca
     4861 ttgcagcact ggggccagat ggtaagccct cccgtatcgt agttatctac acgacgggga
     4921 gtcaggcaac tatggatgaa cgaaatagac agatcgctga gataggtgcc tcactgatta
     4981 agcattggta actgtcagac caagtttact catatatact ttagattgat ttaaaacttc
     5041 atttttaatt taaaaggatc taggtgaaga tcctttttga taatctcatg accaaaatcc
     5101 cttaacgtga gttttcgttc cactgagcgt cagaccccgt agaaaagatc aaaggatctt
     5161 cttgagatcc tttttttctg cgcgtaatct gctgcttgca aacaaaaaaa ccaccgctac
     5221 cagcggtggt ttgtttgccg gatcaagagc taccaactct ttttccgaag gtaactggct
     5281 tcagcagagc gcagatacca aatactgttc ttctagtgta gccgtagtta ggccaccact
     5341 tcaagaactc tgtagcaccg cctacatacc tcgctctgct aatcctgtta ccagtggctg
     5401 ctgccagtgg cgataagtcg tgtcttaccg ggttggactc aagacgatag ttaccggata
     5461 aggcgcagcg gtcgggctga acggggggtt cgtgcacaca gcccagcttg gagcgaacga
     5521 cctacaccga actgagatac ctacagcgtg agctatgaga aagcgccacg cttcccgaag
     5581 ggagaaaggc ggacaggtat ccggtaagcg gcagggtcgg aacaggagag cgcacgaggg
     5641 agcttccagg gggaaacgcc tggtatcttt atagtcctgt cgggtttcgc cacctctgac
     5701 ttgagcgtcg atttttgtga tgctcgtcag gggggcggag cctatggaaa aacgccagca
     5761 acgcggcctt tttacggttc ctggcctttt gctggccttt tgctcacatg ttctttcctg
     5821 cgttatcccc tgattctgtg gataaccgta ttaccgcctt tgagtgagct gataccgctc
     5881 gccgcagccg aacgaccgag cgcagcgagt cagtgagcga ggaagcggaa gagcgcccaa
     5941 tacgcaaacc gcctctcccc gcgcgttggc cgattcatta atgcagctgg cacgacaggt
     6001 ttcccgactg gaaagcgggc agtgagcgca acgcaattaa tgtgagttag ctcactcatt
     6061 aggcacccca ggctttacac tttatgcttc cggctcgtat gttgtgtgga attgtgagcg
     6121 gataacaatt tcacacagga aacagctatg accatgatta cgccaagcgc gcaattaacc
     6181 ctcactaaag ggaacaaaag ctggagctgc aagcttaatg tagtcttatg caatactctt
     6241 gtagtcttgc aacatggtaa cgatgagtta gcaacatgcc ttacaaggag agaaaaagca
     6301 ccgtgcatgc cgattggtgg aagtaaggtg gtacgatcgt gccttattag gaaggcaaca
     6361 gacgggtctg acatggattg gacgaaccac tgaattgccg cattgcagag atattgtatt
     6421 taagtgccta gctcgataca taaacgggtc tctctggtta gaccagatct gagcctggga
     6481 gctctctggc taactaggga acccactgct taagcctcaa taaagcttgc cttgagtgct
     6541 tcaagtagtg tgtgcccgtc tgttgtgtga ctctggtaac tagagatccc tcagaccctt
     6601 ttagtcagtg tggaaaatct ctagcagtgg cgcccgaaca gggacttgaa agcgaaaggg
     6661 aaaccagagg agctctctcg acgcaggact cggcttgctg aagcgcgcac ggcaagaggc
     6721 gaggggcggc gactggtgag tacgccaaaa attttgacta gcggaggcta gaaggagaga
     6781 gatgggtgcg agagcgtcag tattaagcgg gggagaatta gatcgcgatg ggaaaaaatt
     6841 cggttaaggc cagggggaaa gaaaaaatat aaattaaaac atatagtatg ggcaagcagg
     6901 gagctagaac gattcgcagt taatcctggc ctgttagaaa catcagaagg ctgtagacaa
     6961 atactgggac agctacaacc atcccttcag acaggatcag aagaacttag atcattatat
     7021 aatacagtag caaccctcta ttgtgtgcat caaaggatag agataaaaga caccaaggaa
     7081 gctttagaca agatagagga agagcaaaac aaaagtaaga ccaccgcaca gcaagcggcc
     7141 gctgatcttc agacctggag gaggagatat gagggacaat tggagaagtg aattatataa
     7201 atataaagta gtaaaaattg aaccattagg agtagcaccc accaaggcaa agagaagagt
     7261 ggtgcagaga gaaaaaagag cagtgggaat aggagctttg ttccttgggt tcttgggagc
     7321 agcaggaagc actatgggcg cagcgtcaat gacgctgacg gtacaggcca gacaattatt
     7381 gtctggtata gtgcagcagc agaacaattt gctgagggct attgaggcgc aacagcatct
     7441 gttgcaactc acagtctggg gcatcaagca gctccaggca agaatcctgg ctgtggaaag
     7501 atacctaaag gatcaacagc tcctggggat ttggggttgc tctggaaaac tcatttgcac
     7561 cactgctgtg ccttggaatg ctagttggag taataaatct ctggaacaga tttggaatca
     7621 cacgacctgg atggagtggg acagagaaat taacaattac acaagcttaa tacactcctt
     7681 aattgaagaa tcgcaaaacc agcaagaaaa gaatgaacaa gaattattgg aattagataa
     7741 atgggcaagt ttgtggaatt ggtttaacat aacaaattgg ctgtggtata taaaattatt
     7801 cataatgata gtaggaggct tggtaggttt aagaatagtt tttgctgtac tttctatagt
     7861 gaatagagtt aggcagggat attcaccatt atcgtttcag acccacctcc caaccccgag
     7921 gggacccatg cattgcatct caattagtca gcaaccaggt gtggaaagtc cccaggctcc
     7981 ccagcaggca gaagtatgca aagcatgcgt ctcaattagt cagcaaccat agtcccgccc
     8041 ctaactccgc ccatcccgcc cctaactccg cccagttccg cccattctcc gccccatggc
     8101 tgactaattt tttttattta tgcagaggcc gaggccgcct cggcctctga gctattccag
     8161 aagtagtgag gaggcttttt tggaggccta ggcttttgca aaaagctttc tagaggtacc
     8221 accggagctt accatgaccg agtacaagcc cacggtgcgc ctcgccaccc gcgacgacgt
     8281 ccccagggcc gtacgcaccc tcgccgccgc gttcgccgac taccccgcca cgcgccacac
     8341 cgtcgatccg gaccgccaca tcgagcgggt caccgagctg caagaactct tcctcacgcg
     8401 cgtcgggctc gacatcggca aggtgtgggt cgcggacgac ggcgccgccg tggcggtctg
     8461 gaccacgccg gagagcgtcg aagcgggggc ggtgttcgcc gagatcggcc cgcgcatggc
     8521 cgagttgagc ggttcccggc tggccgcgca gcaacagatg gaaggcctcc tggcgccgca
     8581 ccggcccaag gagcccgcgt ggttcctggc caccgtcggc gtctcgcccg accaccaggg
     8641 caagggtctg ggcagcgccg tcgtgctccc cggagtggag gcggccgagc gcgccggggt
     8701 gcccgccttc ctggagacct ccgcgccccg caacctcccc ttctacgagc ggctcggctt
     8761 caccgtcacc gccgacgtcg aggtgcccga aggaccgcgc acctggtgca tgacccgcaa
     8821 gcccggtgcc agttaacgaa ttctagatct tgagacaaat ggcagtattc atccacaatt
     8881 ttaaaagaaa aggggggatt ggggggtaca gtgcagggga aagaatagta gacataatag
     8941 caacagacat acaaactaaa gaattacaaa aacaaattac aaaaattcaa aattttcggg
     9001 tttattacag ggacagcaga gatccacttt ggcgccggct cgaggcctgc aggtgcaaag
     9061 atggataaag ttttaaacag agaggaatct ttgcagctaa tggaccttct aggtcttgaa
     9121 aggagtggga att
//
