LOCUS       sequence-353394-        9364 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9364)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 9364)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF3330
FEATURES             Location/Qualifiers
     source          1..9364
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        1..1179
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          232..1170
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     1127..1147
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     protein_bind    1199..1223
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(2118..2142)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             2150..2191
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     misc_feature    2302..2890
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(2355..2375)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(2773..2784)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             2962..3195
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    3273..3394
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(3310..3329)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     3364..3383
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      3434..3569
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(3589..3608)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(3590..3608)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(3618..3635)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(3618..3634)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(3627..3649)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      3776..4231
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(3863..3882)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     4073..4094
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        4257..4361
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(4362..4430,4431..5222)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(4580..4599)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      5393..5981
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     5882..5901
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     6135..6152
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    6269..6290
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(6305..6310,6311..6328,6329..6335)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6343..6359
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     6348..6370
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     6367..6383
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     6367..6383
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     6402..6422
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        6404..6422
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        6450..6676
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             6677..6857
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    6904..7029
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    7522..7755
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             7940..7984
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     promoter        8215..8425
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     promoter        8237..8433
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     rep_origin      8284..8419
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             8465..9067
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGAS"
     primer_bind     complement(8465..8484)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     8961..8981
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     misc_feature    9110..9227
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
ORIGIN
        1 ggctccggtg cccgtcagtg ggcagagcgc acatcgccca cagtccccga gaagttgggg
       61 ggaggggtcg gcaattgaac cggtgcctag agaaggtggc gcggggtaaa ctgggaaagt
      121 gatgtcgtgt actggctccg cctttttccc gagggtgggg gagaaccgta tataagtgca
      181 gtagtcgccg tgaacgttct ttttcgcaac gggtttgccg ccagaacaca ggtaagtgcc
      241 gtgtgtggtt cccgcgggcc tggcctcttt acgggttatg gcccttgcgt gccttgaatt
      301 acttccacct ggctgcagta cgtgattctt gatcccgagc ttcgggttgg aagtgggtgg
      361 gagagttcga ggccttgcgc ttaaggagcc ccttcgcctc gtgcttgagt tgaggcctgg
      421 cctgggcgct ggggccgccg cgtgcgaatc tggtggcacc ttcgcgcctg tctcgctgct
      481 ttcgataagt ctctagccat ttaaaatttt tgatgacctg ctgcgacgct ttttttctgg
      541 caagatagtc ttgtaaatgc gggccaagat ctgcacactg gtatttcggt ttttggggcc
      601 gcgggcggcg acggggcccg tgcgtcccag cgcacatgtt cggcgaggcg gggcctgcga
      661 gcgcggccac cgagaatcgg acgggggtag tctcaagctg gccggcctgc tctggtgcct
      721 ggcctcgcgc cgccgtgtat cgccccgccc tgggcggcaa ggctggcccg gtcggcacca
      781 gttgcgtgag cggaaagatg gccgcttccc ggccctgctg cagggagctc aaaatggagg
      841 acgcggcgct cgggagagcg ggcgggtgag tcacccacac aaaggaaaag ggcctttccg
      901 tcctcagccg tcgcttcatg tgactccacg gagtaccggg cgccgtccag gcacctcgat
      961 tagttctcga gcttttggag tacgtcgtct ttaggttggg gggaggggtt ttatgcgatg
     1021 gagtttcccc acactgagtg ggtggagact gaagttaggc cagcttggca cttgatgtaa
     1081 ttctccttgg aatttgccct ttttgagttt ggatcttggt tcattctcaa gcctcagaca
     1141 gtggttcaaa gtttttttct tccatttcag gtgtcgtgag gctagcatcg attgatcaac
     1201 aagtttgtac aaaaaagttg gcaccatgga gttcacggcg tcccccaagc cccaactctc
     1261 ctcccgggcc aacgccttct ccattgccgc gctcatgtcg agcggcggct ctaaggagaa
     1321 ggaggcgacg gagaacacaa tcaaacccct ggagcaattt gtggagaagt cgtcctgtgc
     1381 ccagcccctg ggtgagctga ccagcctgga tgctcatggg gagtttggtg gaggcagtgg
     1441 cagcagcccg tcctcctcct ctctgtgcac tgagccactg atccccacca cccccatcat
     1501 ccccagtgag gaaatggcca aaattgcctg cagcctggag accaaggagc tttgggacaa
     1561 attccatgag ctgggcaccg agatgatcat caccaagtcg ggcaggagga tgtttccaac
     1621 catccgggtg tccttttcgg gggtggatcc tgaggccaag tacatagtcc tgatggacat
     1681 cgtccctgtg gacaacaaga ggtaccgcta cgcctaccac cggtcctcct ggctggtggc
     1741 tggcaaggcc gacccgccgt tgccagccag gctctatgtg catccagatt ctccttttac
     1801 cggtgagcaa ctactcaaac agatggtgtc ttttgaaaag gtgaaactca ccaacaatga
     1861 actggatcaa catggccata taattttgaa ctcaatgcat aagtaccagc caagggtgca
     1921 catcattaag aagaaagacc acacagcctc attgctcaac ctgaagtctg aagaatttag
     1981 aactttcatc tttccagaaa cagtttttac ggcagtcact gcctaccaga atcaactgat
     2041 aacgaagctg aaaatagata gcaatccttt tgccaaagga ttccgggatt cctccaggct
     2101 cactgacatt gagaggttgc caactttctt gtacaaagtg gttgatatcg gtaagcctat
     2161 ccctaaccct ctcctcggtc tcgattctac gtagtaatga actagtccgt aacttgaaag
     2221 tatttcgatt tcttggcttt atatatcttg tggaaaggac gaacctaacc taactacgct
     2281 tgaacgacgc gttaagtcga caatcaacct ctggattaca aaatttgtga aagattgact
     2341 ggtattctta actatgttgc tccttttacg ctatgtggat acgctgcttt aatgcctttg
     2401 tatcatgcta ttgcttcccg tatggctttc attttctcct ccttgtataa atcctggttg
     2461 ctgtctcttt atgaggagtt gtggcccgtt gtcaggcaac gtggcgtggt gtgcactgtg
     2521 tttgctgacg caacccccac tggttggggc attgccacca cctgtcagct cctttccggg
     2581 actttcgctt tccccctccc tattgccacg gcggaactca tcgccgcctg ccttgcccgc
     2641 tgctggacag gggctcggct gttgggcact gacaattccg tggtgttgtc ggggaaatca
     2701 tcgtcctttc cttggctgct cgcctgtgtt gccacctgga ttctgcgcgg gacgtccttc
     2761 tgctacgtcc cttcggccct caatccagcg gaccttcctt cccgcggcct gctgccggct
     2821 ctgcggcctc ttccgcgtct tcgccttcgc cctcagacga gtcggatctc cctttgggcc
     2881 gcctccccgc gtcgacttta agaccaatga cttacaaggc agctgtagat cttagccact
     2941 ttttaaaaga aaagggggga ctggaagggc taattcactc ccaacgaaga caagatctgc
     3001 tttttgcttg tactgggtct ctctggttag accagatctg agcctgggag ctctctggct
     3061 aactagggaa cccactgctt aagcctcaat aaagcttgcc ttgagtgctt caagtagtgt
     3121 gtgcccgtct gttgtgtgac tctggtaact agagatccct cagacccttt tagtcagtgt
     3181 ggaaaatctc tagcagtacg tatagtagtt catgtcatct tattattcag tatttataac
     3241 ttgcaaagaa atgaatatca gagagtgaga ggaacttgtt tattgcagct tataatggtt
     3301 acaaataaag caatagcatc acaaatttca caaataaagc atttttttca ctgcattcta
     3361 gttgtggttt gtccaaactc atcaatgtat cttatcatgt ctggctctag ctatcccgcc
     3421 cctaactccg cccatcccgc ccctaactcc gcccagttcc gcccattctc cgccccatgg
     3481 ctgactaatt ttttttattt atgcagaggc cgaggccgcc tcggcctctg agctattcca
     3541 gaagtagtga ggaggctttt ttggaggcct agggacgtac ccaattcgcc ctatagtgag
     3601 tcgtattacg cgcgctcact ggccgtcgtt ttacaacgtc gtgactggga aaaccctggc
     3661 gttacccaac ttaatcgcct tgcagcacat ccccctttcg ccagctggcg taatagcgaa
     3721 gaggcccgca ccgatcgccc ttcccaacag ttgcgcagcc tgaatggcga atgggacgcg
     3781 ccctgtagcg gcgcattaag cgcggcgggt gtggtggtta cgcgcagcgt gaccgctaca
     3841 cttgccagcg ccctagcgcc cgctcctttc gctttcttcc cttcctttct cgccacgttc
     3901 gccggctttc cccgtcaagc tctaaatcgg gggctccctt tagggttccg atttagtgct
     3961 ttacggcacc tcgaccccaa aaaacttgat tagggtgatg gttcacgtag tgggccatcg
     4021 ccctgataga cggtttttcg ccctttgacg ttggagtcca cgttctttaa tagtggactc
     4081 ttgttccaaa ctggaacaac actcaaccct atctcggtct attcttttga tttataaggg
     4141 attttgccga tttcggccta ttggttaaaa aatgagctga tttaacaaaa atttaacgcg
     4201 aattttaaca aaatattaac gcttacaatt taggtggcac ttttcgggga aatgtgcgcg
     4261 gaacccctat ttgtttattt ttctaaatac attcaaatat gtatccgctc atgagacaat
     4321 aaccctgata aatgcttcaa taatattgaa aaaggaagag tatgagtatt caacatttcc
     4381 gtgtcgccct tattcccttt tttgcggcat tttgccttcc tgtttttgct cacccagaaa
     4441 cgctggtgaa agtaaaagat gctgaagatc agttgggtgc acgagtgggt tacatcgaac
     4501 tggatctcaa cagcggtaag atccttgaga gttttcgccc cgaagaacgt tttccaatga
     4561 tgagcacttt taaagttctg ctatgtggcg cggtattatc ccgtattgac gccgggcaag
     4621 agcaactcgg tcgccgcata cactattctc agaatgactt ggttgagtac tcaccagtca
     4681 cagaaaagca tcttacggat ggcatgacag taagagaatt atgcagtgct gccataacca
     4741 tgagtgataa cactgcggcc aacttacttc tgacaacgat cggaggaccg aaggagctaa
     4801 ccgctttttt gcacaacatg ggggatcatg taactcgcct tgatcgttgg gaaccggagc
     4861 tgaatgaagc cataccaaac gacgagcgtg acaccacgat gcctgtagca atggcaacaa
     4921 cgttgcgcaa actattaact ggcgaactac ttactctagc ttcccggcaa caattaatag
     4981 actggatgga ggcggataaa gttgcaggac cacttctgcg ctcggccctt ccggctggct
     5041 ggtttattgc tgataaatct ggagccggtg agcgtgggtc tcgcggtatc attgcagcac
     5101 tggggccaga tggtaagccc tcccgtatcg tagttatcta cacgacgggg agtcaggcaa
     5161 ctatggatga acgaaataga cagatcgctg agataggtgc ctcactgatt aagcattggt
     5221 aactgtcaga ccaagtttac tcatatatac tttagattga tttaaaactt catttttaat
     5281 ttaaaaggat ctaggtgaag atcctttttg ataatctcat gaccaaaatc ccttaacgtg
     5341 agttttcgtt ccactgagcg tcagaccccg tagaaaagat caaaggatct tcttgagatc
     5401 ctttttttct gcgcgtaatc tgctgcttgc aaacaaaaaa accaccgcta ccagcggtgg
     5461 tttgtttgcc ggatcaagag ctaccaactc tttttccgaa ggtaactggc ttcagcagag
     5521 cgcagatacc aaatactgtt cttctagtgt agccgtagtt aggccaccac ttcaagaact
     5581 ctgtagcacc gcctacatac ctcgctctgc taatcctgtt accagtggct gctgccagtg
     5641 gcgataagtc gtgtcttacc gggttggact caagacgata gttaccggat aaggcgcagc
     5701 ggtcgggctg aacggggggt tcgtgcacac agcccagctt ggagcgaacg acctacaccg
     5761 aactgagata cctacagcgt gagctatgag aaagcgccac gcttcccgaa gggagaaagg
     5821 cggacaggta tccggtaagc ggcagggtcg gaacaggaga gcgcacgagg gagcttccag
     5881 ggggaaacgc ctggtatctt tatagtcctg tcgggtttcg ccacctctga cttgagcgtc
     5941 gatttttgtg atgctcgtca ggggggcgga gcctatggaa aaacgccagc aacgcggcct
     6001 ttttacggtt cctggccttt tgctggcctt ttgctcacat gttctttcct gcgttatccc
     6061 ctgattctgt ggataaccgt attaccgcct ttgagtgagc tgataccgct cgccgcagcc
     6121 gaacgaccga gcgcagcgag tcagtgagcg aggaagcgga agagcgccca atacgcaaac
     6181 cgcctctccc cgcgcgttgg ccgattcatt aatgcagctg gcacgacagg tttcccgact
     6241 ggaaagcggg cagtgagcgc aacgcaatta atgtgagtta gctcactcat taggcacccc
     6301 aggctttaca ctttatgctt ccggctcgta tgttgtgtgg aattgtgagc ggataacaat
     6361 ttcacacagg aaacagctat gaccatgatt acgccaagcg cgcaattaac cctcactaaa
     6421 gggaacaaaa gctggagctg caagcttaat gtagtcttat gcaatactct tgtagtcttg
     6481 caacatggta acgatgagtt agcaacatgc cttacaagga gagaaaaagc accgtgcatg
     6541 ccgattggtg gaagtaaggt ggtacgatcg tgccttatta ggaaggcaac agacgggtct
     6601 gacatggatt ggacgaacca ctgaattgcc gcattgcaga gatattgtat ttaagtgcct
     6661 agctcgatac ataaacgggt ctctctggtt agaccagatc tgagcctggg agctctctgg
     6721 ctaactaggg aacccactgc ttaagcctca ataaagcttg ccttgagtgc ttcaagtagt
     6781 gtgtgcccgt ctgttgtgtg actctggtaa ctagagatcc ctcagaccct tttagtcagt
     6841 gtggaaaatc tctagcagtg gcgcccgaac agggacttga aagcgaaagg gaaaccagag
     6901 gagctctctc gacgcaggac tcggcttgct gaagcgcgca cggcaagagg cgaggggcgg
     6961 cgactggtga gtacgccaaa aattttgact agcggaggct agaaggagag agatgggtgc
     7021 gagagcgtca gtattaagcg ggggagaatt agatcgcgat gggaaaaaat tcggttaagg
     7081 ccagggggaa agaaaaaata taaattaaaa catatagtat gggcaagcag ggagctagaa
     7141 cgattcgcag ttaatcctgg cctgttagaa acatcagaag gctgtagaca aatactggga
     7201 cagctacaac catcccttca gacaggatca gaagaactta gatcattata taatacagta
     7261 gcaaccctct attgtgtgca tcaaaggata gagataaaag acaccaagga agctttagac
     7321 aagatagagg aagagcaaaa caaaagtaag accaccgcac agcaagcggc cgctgatctt
     7381 cagacctgga ggaggagata tgagggacaa ttggagaagt gaattatata aatataaagt
     7441 agtaaaaatt gaaccattag gagtagcacc caccaaggca aagagaagag tggtgcagag
     7501 agaaaaaaga gcagtgggaa taggagcttt gttccttggg ttcttgggag cagcaggaag
     7561 cactatgggc gcagcgtcaa tgacgctgac ggtacaggcc agacaattat tgtctggtat
     7621 agtgcagcag cagaacaatt tgctgagggc tattgaggcg caacagcatc tgttgcaact
     7681 cacagtctgg ggcatcaagc agctccaggc aagaatcctg gctgtggaaa gatacctaaa
     7741 ggatcaacag ctcctgggga tttggggttg ctctggaaaa ctcatttgca ccactgctgt
     7801 gccttggaat gctagttgga gtaataaatc tctggaacag atttggaatc acacgacctg
     7861 gatggagtgg gacagagaaa ttaacaatta cacaagctta atacactcct taattgaaga
     7921 atcgcaaaac cagcaagaaa agaatgaaca agaattattg gaattagata aatgggcaag
     7981 tttgtggaat tggtttaaca taacaaattg gctgtggtat ataaaattat tcataatgat
     8041 agtaggaggc ttggtaggtt taagaatagt ttttgctgta ctttctatag tgaatagagt
     8101 taggcaggga tattcaccat tatcgtttca gacccacctc ccaaccccga ggggacccat
     8161 gcattgcatc tcaattagtc agcaaccagg tgtggaaagt ccccaggctc cccagcaggc
     8221 agaagtatgc aaagcatgcg tctcaattag tcagcaacca tagtcccgcc cctaactccg
     8281 cccatcccgc ccctaactcc gcccagttcc gcccattctc cgccccatgg ctgactaatt
     8341 ttttttattt atgcagaggc cgaggccgcc tcggcctctg agctattcca gaagtagtga
     8401 ggaggctttt ttggaggcct aggcttttgc aaaaagcttt ctagaggtac caccggagct
     8461 taccatgacc gagtacaagc ccacggtgcg cctcgccacc cgcgacgacg tccccagggc
     8521 cgtacgcacc ctcgccgccg cgttcgccga ctaccccgcc acgcgccaca ccgtcgatcc
     8581 ggaccgccac atcgagcggg tcaccgagct gcaagaactc ttcctcacgc gcgtcgggct
     8641 cgacatcggc aaggtgtggg tcgcggacga cggcgccgcc gtggcggtct ggaccacgcc
     8701 ggagagcgtc gaagcggggg cggtgttcgc cgagatcggc ccgcgcatgg ccgagttgag
     8761 cggttcccgg ctggccgcgc agcaacagat ggaaggcctc ctggcgccgc accggcccaa
     8821 ggagcccgcg tggttcctgg ccaccgtcgg cgtctcgccc gaccaccagg gcaagggtct
     8881 gggcagcgcc gtcgtgctcc ccggagtgga ggcggccgag cgcgccgggg tgcccgcctt
     8941 cctggagacc tccgcgcccc gcaacctccc cttctacgag cggctcggct tcaccgtcac
     9001 cgccgacgtc gaggtgcccg aaggaccgcg cacctggtgc atgacccgca agcccggtgc
     9061 cagttaacga attctagatc ttgagacaaa tggcagtatt catccacaat tttaaaagaa
     9121 aaggggggat tggggggtac agtgcagggg aaagaatagt agacataata gcaacagaca
     9181 tacaaactaa agaattacaa aaacaaatta caaaaattca aaattttcgg gtttattaca
     9241 gggacagcag agatccactt tggcgccggc tcgaggcctg caggtgcaaa gatggataaa
     9301 gttttaaaca gagaggaatc tttgcagcta atggaccttc taggtcttga aaggagtggg
     9361 aatt
//
