LOCUS       sequence-353396-        9520 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9520)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 9520)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF3366
FEATURES             Location/Qualifiers
     source          1..9520
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        1..1179
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          232..1170
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     1127..1147
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     protein_bind    1199..1223
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             complement(2078..2089)
                     /codon_start=1
                     /product="WELQut protease recognition and cleavage site"
                     /label=WELQut site
                     /translation="WELQ"
     protein_bind    complement(2274..2298)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             2306..2347
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     misc_feature    2458..3046
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(2511..2531)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(2929..2940)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             3118..3351
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    3429..3550
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(3466..3485)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     3520..3539
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      3590..3725
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(3745..3764)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(3746..3764)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(3774..3791)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(3774..3790)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(3783..3805)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      3932..4387
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(4019..4038)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     4229..4250
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        4413..4517
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(4518..4586,4587..5378)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(4736..4755)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      5549..6137
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     6038..6057
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     6291..6308
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    6425..6446
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(6461..6466,6467..6484,6485..6491)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6499..6515
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     6504..6526
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     6523..6539
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     6523..6539
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     6558..6578
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        6560..6578
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        6606..6832
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             6833..7013
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    7060..7185
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    7678..7911
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             8096..8140
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     promoter        8371..8581
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     promoter        8393..8589
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     rep_origin      8440..8575
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             8621..9223
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGAS"
     primer_bind     complement(8621..8640)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     9117..9137
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     misc_feature    9266..9383
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
ORIGIN
        1 ggctccggtg cccgtcagtg ggcagagcgc acatcgccca cagtccccga gaagttgggg
       61 ggaggggtcg gcaattgaac cggtgcctag agaaggtggc gcggggtaaa ctgggaaagt
      121 gatgtcgtgt actggctccg cctttttccc gagggtgggg gagaaccgta tataagtgca
      181 gtagtcgccg tgaacgttct ttttcgcaac gggtttgccg ccagaacaca ggtaagtgcc
      241 gtgtgtggtt cccgcgggcc tggcctcttt acgggttatg gcccttgcgt gccttgaatt
      301 acttccacct ggctgcagta cgtgattctt gatcccgagc ttcgggttgg aagtgggtgg
      361 gagagttcga ggccttgcgc ttaaggagcc ccttcgcctc gtgcttgagt tgaggcctgg
      421 cctgggcgct ggggccgccg cgtgcgaatc tggtggcacc ttcgcgcctg tctcgctgct
      481 ttcgataagt ctctagccat ttaaaatttt tgatgacctg ctgcgacgct ttttttctgg
      541 caagatagtc ttgtaaatgc gggccaagat ctgcacactg gtatttcggt ttttggggcc
      601 gcgggcggcg acggggcccg tgcgtcccag cgcacatgtt cggcgaggcg gggcctgcga
      661 gcgcggccac cgagaatcgg acgggggtag tctcaagctg gccggcctgc tctggtgcct
      721 ggcctcgcgc cgccgtgtat cgccccgccc tgggcggcaa ggctggcccg gtcggcacca
      781 gttgcgtgag cggaaagatg gccgcttccc ggccctgctg cagggagctc aaaatggagg
      841 acgcggcgct cgggagagcg ggcgggtgag tcacccacac aaaggaaaag ggcctttccg
      901 tcctcagccg tcgcttcatg tgactccacg gagtaccggg cgccgtccag gcacctcgat
      961 tagttctcga gcttttggag tacgtcgtct ttaggttggg gggaggggtt ttatgcgatg
     1021 gagtttcccc acactgagtg ggtggagact gaagttaggc cagcttggca cttgatgtaa
     1081 ttctccttgg aatttgccct ttttgagttt ggatcttggt tcattctcaa gcctcagaca
     1141 gtggttcaaa gtttttttct tccatttcag gtgtcgtgag gctagcatcg attgatcaac
     1201 aagtttgtac aaaaaagttg gcatgctggg ctcagtgaag atggaggccc atgacctggc
     1261 cgagtggagc tactacccgg aggcgggcga ggtctactcg ccggtgaccc cagtgcccac
     1321 catggccccc ctcaactcct acatgaccct gaatcctcta agctctccct atccccctgg
     1381 ggggctccct gcctccccac tgccctcagg acccctggca cccccagcac ctgcagcccc
     1441 cctggggccc actttcccag gcctgggtgt cagcggtggc agcagcagct ccgggtacgg
     1501 ggccccgggt cctgggctgg tgcacgggaa ggagatgccg aaggggtatc ggcggcccct
     1561 ggcacacgcc aagccaccgt attcctatat ctcactcatc accatggcca tccagcaggc
     1621 gccgggcaag atgctgacct tgagtgaaat ctaccagtgg atcatggacc tcttccctta
     1681 ctaccgggag aatcagcagc gctggcagaa ctccattcgc cactcgctgt ctttcaacga
     1741 ctgcttcgtc aaggtggcgc gttccccaga caagcctggc aagggctcct actgggccct
     1801 acaccccagc tcagggaaca tgtttgagaa tggctgctac ctgcgccgcc agaaacgctt
     1861 caagctggag gagaaggtga aaaaaggggg cagcggggct gccaccacca ccaggaacgg
     1921 gacagggtct gctgcctcga ccaccacccc cgcggccaca gtcacctccc cgccccagcc
     1981 cccgcctcca gcccctgagc ctgaggccca gggcggggaa gatgtggggg ctctggactg
     2041 tggctcaccc gcttcctcca caccctattt cactggcctg gagctcccag gggagctgaa
     2101 gctggacgcg ccctacaact tcaaccaccc tttctccatc aacaacctaa tgtcagaaca
     2161 gacaccagca cctcccaaac tggacgtggg gtttgggggc tacggggctg aaggtgggga
     2221 gcctggagtc tactaccagg gcctctattc ccgctctttg cttaatgcat cctacccaac
     2281 tttcttgtac aaagtggttg atatcggtaa gcctatccct aaccctctcc tcggtctcga
     2341 ttctacgtag taatgaacta gtccgtaact tgaaagtatt tcgatttctt ggctttatat
     2401 atcttgtgga aaggacgaca cgaagcagga actcgataac agacgcgtta agtcgacaat
     2461 caacctctgg attacaaaat ttgtgaaaga ttgactggta ttcttaacta tgttgctcct
     2521 tttacgctat gtggatacgc tgctttaatg cctttgtatc atgctattgc ttcccgtatg
     2581 gctttcattt tctcctcctt gtataaatcc tggttgctgt ctctttatga ggagttgtgg
     2641 cccgttgtca ggcaacgtgg cgtggtgtgc actgtgtttg ctgacgcaac ccccactggt
     2701 tggggcattg ccaccacctg tcagctcctt tccgggactt tcgctttccc cctccctatt
     2761 gccacggcgg aactcatcgc cgcctgcctt gcccgctgct ggacaggggc tcggctgttg
     2821 ggcactgaca attccgtggt gttgtcgggg aaatcatcgt cctttccttg gctgctcgcc
     2881 tgtgttgcca cctggattct gcgcgggacg tccttctgct acgtcccttc ggccctcaat
     2941 ccagcggacc ttccttcccg cggcctgctg ccggctctgc ggcctcttcc gcgtcttcgc
     3001 cttcgccctc agacgagtcg gatctccctt tgggccgcct ccccgcgtcg actttaagac
     3061 caatgactta caaggcagct gtagatctta gccacttttt aaaagaaaag gggggactgg
     3121 aagggctaat tcactcccaa cgaagacaag atctgctttt tgcttgtact gggtctctct
     3181 ggttagacca gatctgagcc tgggagctct ctggctaact agggaaccca ctgcttaagc
     3241 ctcaataaag cttgccttga gtgcttcaag tagtgtgtgc ccgtctgttg tgtgactctg
     3301 gtaactagag atccctcaga cccttttagt cagtgtggaa aatctctagc agtacgtata
     3361 gtagttcatg tcatcttatt attcagtatt tataacttgc aaagaaatga atatcagaga
     3421 gtgagaggaa cttgtttatt gcagcttata atggttacaa ataaagcaat agcatcacaa
     3481 atttcacaaa taaagcattt ttttcactgc attctagttg tggtttgtcc aaactcatca
     3541 atgtatctta tcatgtctgg ctctagctat cccgccccta actccgccca tcccgcccct
     3601 aactccgccc agttccgccc attctccgcc ccatggctga ctaatttttt ttatttatgc
     3661 agaggccgag gccgcctcgg cctctgagct attccagaag tagtgaggag gcttttttgg
     3721 aggcctaggg acgtacccaa ttcgccctat agtgagtcgt attacgcgcg ctcactggcc
     3781 gtcgttttac aacgtcgtga ctgggaaaac cctggcgtta cccaacttaa tcgccttgca
     3841 gcacatcccc ctttcgccag ctggcgtaat agcgaagagg cccgcaccga tcgcccttcc
     3901 caacagttgc gcagcctgaa tggcgaatgg gacgcgccct gtagcggcgc attaagcgcg
     3961 gcgggtgtgg tggttacgcg cagcgtgacc gctacacttg ccagcgccct agcgcccgct
     4021 cctttcgctt tcttcccttc ctttctcgcc acgttcgccg gctttccccg tcaagctcta
     4081 aatcgggggc tccctttagg gttccgattt agtgctttac ggcacctcga ccccaaaaaa
     4141 cttgattagg gtgatggttc acgtagtggg ccatcgccct gatagacggt ttttcgccct
     4201 ttgacgttgg agtccacgtt ctttaatagt ggactcttgt tccaaactgg aacaacactc
     4261 aaccctatct cggtctattc ttttgattta taagggattt tgccgatttc ggcctattgg
     4321 ttaaaaaatg agctgattta acaaaaattt aacgcgaatt ttaacaaaat attaacgctt
     4381 acaatttagg tggcactttt cggggaaatg tgcgcggaac ccctatttgt ttatttttct
     4441 aaatacattc aaatatgtat ccgctcatga gacaataacc ctgataaatg cttcaataat
     4501 attgaaaaag gaagagtatg agtattcaac atttccgtgt cgcccttatt cccttttttg
     4561 cggcattttg ccttcctgtt tttgctcacc cagaaacgct ggtgaaagta aaagatgctg
     4621 aagatcagtt gggtgcacga gtgggttaca tcgaactgga tctcaacagc ggtaagatcc
     4681 ttgagagttt tcgccccgaa gaacgttttc caatgatgag cacttttaaa gttctgctat
     4741 gtggcgcggt attatcccgt attgacgccg ggcaagagca actcggtcgc cgcatacact
     4801 attctcagaa tgacttggtt gagtactcac cagtcacaga aaagcatctt acggatggca
     4861 tgacagtaag agaattatgc agtgctgcca taaccatgag tgataacact gcggccaact
     4921 tacttctgac aacgatcgga ggaccgaagg agctaaccgc ttttttgcac aacatggggg
     4981 atcatgtaac tcgccttgat cgttgggaac cggagctgaa tgaagccata ccaaacgacg
     5041 agcgtgacac cacgatgcct gtagcaatgg caacaacgtt gcgcaaacta ttaactggcg
     5101 aactacttac tctagcttcc cggcaacaat taatagactg gatggaggcg gataaagttg
     5161 caggaccact tctgcgctcg gcccttccgg ctggctggtt tattgctgat aaatctggag
     5221 ccggtgagcg tgggtctcgc ggtatcattg cagcactggg gccagatggt aagccctccc
     5281 gtatcgtagt tatctacacg acggggagtc aggcaactat ggatgaacga aatagacaga
     5341 tcgctgagat aggtgcctca ctgattaagc attggtaact gtcagaccaa gtttactcat
     5401 atatacttta gattgattta aaacttcatt tttaatttaa aaggatctag gtgaagatcc
     5461 tttttgataa tctcatgacc aaaatccctt aacgtgagtt ttcgttccac tgagcgtcag
     5521 accccgtaga aaagatcaaa ggatcttctt gagatccttt ttttctgcgc gtaatctgct
     5581 gcttgcaaac aaaaaaacca ccgctaccag cggtggtttg tttgccggat caagagctac
     5641 caactctttt tccgaaggta actggcttca gcagagcgca gataccaaat actgttcttc
     5701 tagtgtagcc gtagttaggc caccacttca agaactctgt agcaccgcct acatacctcg
     5761 ctctgctaat cctgttacca gtggctgctg ccagtggcga taagtcgtgt cttaccgggt
     5821 tggactcaag acgatagtta ccggataagg cgcagcggtc gggctgaacg gggggttcgt
     5881 gcacacagcc cagcttggag cgaacgacct acaccgaact gagataccta cagcgtgagc
     5941 tatgagaaag cgccacgctt cccgaaggga gaaaggcgga caggtatccg gtaagcggca
     6001 gggtcggaac aggagagcgc acgagggagc ttccaggggg aaacgcctgg tatctttata
     6061 gtcctgtcgg gtttcgccac ctctgacttg agcgtcgatt tttgtgatgc tcgtcagggg
     6121 ggcggagcct atggaaaaac gccagcaacg cggccttttt acggttcctg gccttttgct
     6181 ggccttttgc tcacatgttc tttcctgcgt tatcccctga ttctgtggat aaccgtatta
     6241 ccgcctttga gtgagctgat accgctcgcc gcagccgaac gaccgagcgc agcgagtcag
     6301 tgagcgagga agcggaagag cgcccaatac gcaaaccgcc tctccccgcg cgttggccga
     6361 ttcattaatg cagctggcac gacaggtttc ccgactggaa agcgggcagt gagcgcaacg
     6421 caattaatgt gagttagctc actcattagg caccccaggc tttacacttt atgcttccgg
     6481 ctcgtatgtt gtgtggaatt gtgagcggat aacaatttca cacaggaaac agctatgacc
     6541 atgattacgc caagcgcgca attaaccctc actaaaggga acaaaagctg gagctgcaag
     6601 cttaatgtag tcttatgcaa tactcttgta gtcttgcaac atggtaacga tgagttagca
     6661 acatgcctta caaggagaga aaaagcaccg tgcatgccga ttggtggaag taaggtggta
     6721 cgatcgtgcc ttattaggaa ggcaacagac gggtctgaca tggattggac gaaccactga
     6781 attgccgcat tgcagagata ttgtatttaa gtgcctagct cgatacataa acgggtctct
     6841 ctggttagac cagatctgag cctgggagct ctctggctaa ctagggaacc cactgcttaa
     6901 gcctcaataa agcttgcctt gagtgcttca agtagtgtgt gcccgtctgt tgtgtgactc
     6961 tggtaactag agatccctca gaccctttta gtcagtgtgg aaaatctcta gcagtggcgc
     7021 ccgaacaggg acttgaaagc gaaagggaaa ccagaggagc tctctcgacg caggactcgg
     7081 cttgctgaag cgcgcacggc aagaggcgag gggcggcgac tggtgagtac gccaaaaatt
     7141 ttgactagcg gaggctagaa ggagagagat gggtgcgaga gcgtcagtat taagcggggg
     7201 agaattagat cgcgatggga aaaaattcgg ttaaggccag ggggaaagaa aaaatataaa
     7261 ttaaaacata tagtatgggc aagcagggag ctagaacgat tcgcagttaa tcctggcctg
     7321 ttagaaacat cagaaggctg tagacaaata ctgggacagc tacaaccatc ccttcagaca
     7381 ggatcagaag aacttagatc attatataat acagtagcaa ccctctattg tgtgcatcaa
     7441 aggatagaga taaaagacac caaggaagct ttagacaaga tagaggaaga gcaaaacaaa
     7501 agtaagacca ccgcacagca agcggccgct gatcttcaga cctggaggag gagatatgag
     7561 ggacaattgg agaagtgaat tatataaata taaagtagta aaaattgaac cattaggagt
     7621 agcacccacc aaggcaaaga gaagagtggt gcagagagaa aaaagagcag tgggaatagg
     7681 agctttgttc cttgggttct tgggagcagc aggaagcact atgggcgcag cgtcaatgac
     7741 gctgacggta caggccagac aattattgtc tggtatagtg cagcagcaga acaatttgct
     7801 gagggctatt gaggcgcaac agcatctgtt gcaactcaca gtctggggca tcaagcagct
     7861 ccaggcaaga atcctggctg tggaaagata cctaaaggat caacagctcc tggggatttg
     7921 gggttgctct ggaaaactca tttgcaccac tgctgtgcct tggaatgcta gttggagtaa
     7981 taaatctctg gaacagattt ggaatcacac gacctggatg gagtgggaca gagaaattaa
     8041 caattacaca agcttaatac actccttaat tgaagaatcg caaaaccagc aagaaaagaa
     8101 tgaacaagaa ttattggaat tagataaatg ggcaagtttg tggaattggt ttaacataac
     8161 aaattggctg tggtatataa aattattcat aatgatagta ggaggcttgg taggtttaag
     8221 aatagttttt gctgtacttt ctatagtgaa tagagttagg cagggatatt caccattatc
     8281 gtttcagacc cacctcccaa ccccgagggg acccatgcat tgcatctcaa ttagtcagca
     8341 accaggtgtg gaaagtcccc aggctcccca gcaggcagaa gtatgcaaag catgcgtctc
     8401 aattagtcag caaccatagt cccgccccta actccgccca tcccgcccct aactccgccc
     8461 agttccgccc attctccgcc ccatggctga ctaatttttt ttatttatgc agaggccgag
     8521 gccgcctcgg cctctgagct attccagaag tagtgaggag gcttttttgg aggcctaggc
     8581 ttttgcaaaa agctttctag aggtaccacc ggagcttacc atgaccgagt acaagcccac
     8641 ggtgcgcctc gccacccgcg acgacgtccc cagggccgta cgcaccctcg ccgccgcgtt
     8701 cgccgactac cccgccacgc gccacaccgt cgatccggac cgccacatcg agcgggtcac
     8761 cgagctgcaa gaactcttcc tcacgcgcgt cgggctcgac atcggcaagg tgtgggtcgc
     8821 ggacgacggc gccgccgtgg cggtctggac cacgccggag agcgtcgaag cgggggcggt
     8881 gttcgccgag atcggcccgc gcatggccga gttgagcggt tcccggctgg ccgcgcagca
     8941 acagatggaa ggcctcctgg cgccgcaccg gcccaaggag cccgcgtggt tcctggccac
     9001 cgtcggcgtc tcgcccgacc accagggcaa gggtctgggc agcgccgtcg tgctccccgg
     9061 agtggaggcg gccgagcgcg ccggggtgcc cgccttcctg gagacctccg cgccccgcaa
     9121 cctccccttc tacgagcggc tcggcttcac cgtcaccgcc gacgtcgagg tgcccgaagg
     9181 accgcgcacc tggtgcatga cccgcaagcc cggtgccagt taacgaattc tagatcttga
     9241 gacaaatggc agtattcatc cacaatttta aaagaaaagg ggggattggg gggtacagtg
     9301 caggggaaag aatagtagac ataatagcaa cagacataca aactaaagaa ttacaaaaac
     9361 aaattacaaa aattcaaaat tttcgggttt attacaggga cagcagagat ccactttggc
     9421 gccggctcga ggcctgcagg tgcaaagatg gataaagttt taaacagaga ggaatctttg
     9481 cagctaatgg accttctagg tcttgaaagg agtgggaatt
//
