LOCUS       sequence-353400-       10033 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10033)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 10033)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF1119
FEATURES             Location/Qualifiers
     source          1..10033
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        1..1179
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          232..1170
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     1127..1147
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     misc_feature    2971..3559
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(3024..3044)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(3442..3453)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             3631..3864
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    3942..4063
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(3979..3998)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     4033..4052
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      4103..4238
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(4258..4277)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(4259..4277)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(4287..4304)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(4287..4303)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(4296..4318)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      4445..4900
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(4532..4551)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     4742..4763
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        4926..5030
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(5031..5099,5100..5891)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(5249..5268)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      6062..6650
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     6551..6570
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     6804..6821
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    6938..6959
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(6974..6979,6980..6997,6998..7004)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7012..7028
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     7017..7039
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     7036..7052
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     7036..7052
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     7071..7091
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        7073..7091
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        7119..7345
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             7346..7526
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    7573..7698
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    8191..8424
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             8609..8653
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     promoter        8884..9094
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     promoter        8906..9102
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     rep_origin      8953..9088
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             9134..9736
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGAS"
     primer_bind     complement(9134..9153)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     9630..9650
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     misc_feature    9779..9896
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
ORIGIN
        1 ggctccggtg cccgtcagtg ggcagagcgc acatcgccca cagtccccga gaagttgggg
       61 ggaggggtcg gcaattgaac cggtgcctag agaaggtggc gcggggtaaa ctgggaaagt
      121 gatgtcgtgt actggctccg cctttttccc gagggtgggg gagaaccgta tataagtgca
      181 gtagtcgccg tgaacgttct ttttcgcaac gggtttgccg ccagaacaca ggtaagtgcc
      241 gtgtgtggtt cccgcgggcc tggcctcttt acgggttatg gcccttgcgt gccttgaatt
      301 acttccacct ggctgcagta cgtgattctt gatcccgagc ttcgggttgg aagtgggtgg
      361 gagagttcga ggccttgcgc ttaaggagcc ccttcgcctc gtgcttgagt tgaggcctgg
      421 cctgggcgct ggggccgccg cgtgcgaatc tggtggcacc ttcgcgcctg tctcgctgct
      481 ttcgataagt ctctagccat ttaaaatttt tgatgacctg ctgcgacgct ttttttctgg
      541 caagatagtc ttgtaaatgc gggccaagat ctgcacactg gtatttcggt ttttggggcc
      601 gcgggcggcg acggggcccg tgcgtcccag cgcacatgtt cggcgaggcg gggcctgcga
      661 gcgcggccac cgagaatcgg acgggggtag tctcaagctg gccggcctgc tctggtgcct
      721 ggcctcgcgc cgccgtgtat cgccccgccc tgggcggcaa ggctggcccg gtcggcacca
      781 gttgcgtgag cggaaagatg gccgcttccc ggccctgctg cagggagctc aaaatggagg
      841 acgcggcgct cgggagagcg ggcgggtgag tcacccacac aaaggaaaag ggcctttccg
      901 tcctcagccg tcgcttcatg tgactccacg gagtaccggg cgccgtccag gcacctcgat
      961 tagttctcga gcttttggag tacgtcgtct ttaggttggg gggaggggtt ttatgcgatg
     1021 gagtttcccc acactgagtg ggtggagact gaagttaggc cagcttggca cttgatgtaa
     1081 ttctccttgg aatttgccct ttttgagttt ggatcttggt tcattctcaa gcctcagaca
     1141 gtggttcaaa gtttttttct tccatttcag gtgtcgtgag gctagcaccg ccaccatgaa
     1201 tgtgatgtta gagaactaca aaaacctggt cttcttggca ggtattgctg tctctaagca
     1261 agacccgatc accagtctag agcaagaaaa agagccctgg aatatgaaga tatgtgagat
     1321 ggtggatgaa tccccagcta tgtgttcttc ttttaccaga gacctttggc cagagcaaga
     1381 cataaaagat tcttttcaac aagtgatact gagaagacat ggcaaatgtg aacatgagaa
     1441 tttacagtta agaaaaggct ccgcaaatgt ggttgagtgt aaggtgtaca aaaaaggtta
     1501 taatgaacta aaccagtgtt tgacaactac ccagagcaaa atatttccat gtgataaata
     1561 tataaaagtc tttcataaaa ttttcaattc aaatagacac aagacaagac atactggaga
     1621 gaaacctttc aaatgtaaaa aatgtgatga atcattttgc atgcttttac acctacatca
     1681 acataaaaga attcatatta gagagaattc ttaccaatgt gaagaatgtg acaaagtgtt
     1741 taagcggttc tcaaccctta ctagacacaa gagagttcat actggagaga aacccttcaa
     1801 atgtgaagaa tgtggcaaag cttttaagca ctcctcaacc cttactacac ataagatgat
     1861 tcatactgga gagaaaccct acagatgtga agaatgtggc aaagccttct accattcttc
     1921 acaccttact acacataagg taattcatac tggagagaag cccttcaaat gtgaagaatg
     1981 tggtaaagct tttaaccacc cttcagccct tactacacat aagttcattc atgttaaaga
     2041 aaaaccctac aaatgtgaag aatgtgacaa agcttttaac cgattctcat accttactaa
     2101 acataagata attcattctg gagagaaatc ttacaaatgt gaacaatgtg gcaaaggctt
     2161 taactggtct tcaaccctta caaaacataa aagaattcat actggagaga aaccctacaa
     2221 atgtgaagaa tgtggcaaag cctttaatgt gtcttcacac cttactacac ataagatgat
     2281 tcatactgga gagaaaccct acaaatgtga agaatgtggc aaagccttta accactcctc
     2341 aaaacttact atacataaga taattcatac tggagagaaa ccttacaaat gtgaagaatg
     2401 tggcaaagct tttaaccaat cctcaaacct taccaaacat aagataattc atactggaga
     2461 gaaactctac aaatgtgaag aatgtggcaa agcttttaac cgatcctcaa accttactac
     2521 acataagaga attcacactg gagagaaacc ctacaaatgt gaagaatgtg gcaaagcttt
     2581 caaccgatcc tcaaacctta ctaaacataa cataattcat actggagaga aatcttacaa
     2641 atgtgaagaa tgtggtaaag cctttaacca atcctcaact cttactaaac ataggaaaat
     2701 tcagcagggc atggtggctc atgcctgtaa tcccaacact ttgagaggac taggtgagca
     2761 gatcgcgagg tcaggagttc aagaccagcc tggccaacat ggtaaaaccc catctctact
     2821 aaaaatacaa aaatttgctg ggtgtggtgg caggcgcctg tagtaatgaa ctagtccgta
     2881 acttgaaagt atttcgattt cttggcttta tatatcttgt ggaaaggacg attatgcgaa
     2941 tggcttgtta ggtttacgcg ttaagtcgac aatcaacctc tggattacaa aatttgtgaa
     3001 agattgactg gtattcttaa ctatgttgct ccttttacgc tatgtggata cgctgcttta
     3061 atgcctttgt atcatgctat tgcttcccgt atggctttca ttttctcctc cttgtataaa
     3121 tcctggttgc tgtctcttta tgaggagttg tggcccgttg tcaggcaacg tggcgtggtg
     3181 tgcactgtgt ttgctgacgc aacccccact ggttggggca ttgccaccac ctgtcagctc
     3241 ctttccggga ctttcgcttt ccccctccct attgccacgg cggaactcat cgccgcctgc
     3301 cttgcccgct gctggacagg ggctcggctg ttgggcactg acaattccgt ggtgttgtcg
     3361 gggaaatcat cgtcctttcc ttggctgctc gcctgtgttg ccacctggat tctgcgcggg
     3421 acgtccttct gctacgtccc ttcggccctc aatccagcgg accttccttc ccgcggcctg
     3481 ctgccggctc tgcggcctct tccgcgtctt cgccttcgcc ctcagacgag tcggatctcc
     3541 ctttgggccg cctccccgcg tcgactttaa gaccaatgac ttacaaggca gctgtagatc
     3601 ttagccactt tttaaaagaa aaggggggac tggaagggct aattcactcc caacgaagac
     3661 aagatctgct ttttgcttgt actgggtctc tctggttaga ccagatctga gcctgggagc
     3721 tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct tgagtgcttc
     3781 aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc agaccctttt
     3841 agtcagtgtg gaaaatctct agcagtacgt atagtagttc atgtcatctt attattcagt
     3901 atttataact tgcaaagaaa tgaatatcag agagtgagag gaacttgttt attgcagctt
     3961 ataatggtta caaataaagc aatagcatca caaatttcac aaataaagca tttttttcac
     4021 tgcattctag ttgtggtttg tccaaactca tcaatgtatc ttatcatgtc tggctctagc
     4081 tatcccgccc ctaactccgc ccatcccgcc cctaactccg cccagttccg cccattctcc
     4141 gccccatggc tgactaattt tttttattta tgcagaggcc gaggccgcct cggcctctga
     4201 gctattccag aagtagtgag gaggcttttt tggaggccta gggacgtacc caattcgccc
     4261 tatagtgagt cgtattacgc gcgctcactg gccgtcgttt tacaacgtcg tgactgggaa
     4321 aaccctggcg ttacccaact taatcgcctt gcagcacatc cccctttcgc cagctggcgt
     4381 aatagcgaag aggcccgcac cgatcgccct tcccaacagt tgcgcagcct gaatggcgaa
     4441 tgggacgcgc cctgtagcgg cgcattaagc gcggcgggtg tggtggttac gcgcagcgtg
     4501 accgctacac ttgccagcgc cctagcgccc gctcctttcg ctttcttccc ttcctttctc
     4561 gccacgttcg ccggctttcc ccgtcaagct ctaaatcggg ggctcccttt agggttccga
     4621 tttagtgctt tacggcacct cgaccccaaa aaacttgatt agggtgatgg ttcacgtagt
     4681 gggccatcgc cctgatagac ggtttttcgc cctttgacgt tggagtccac gttctttaat
     4741 agtggactct tgttccaaac tggaacaaca ctcaacccta tctcggtcta ttcttttgat
     4801 ttataaggga ttttgccgat ttcggcctat tggttaaaaa atgagctgat ttaacaaaaa
     4861 tttaacgcga attttaacaa aatattaacg cttacaattt aggtggcact tttcggggaa
     4921 atgtgcgcgg aacccctatt tgtttatttt tctaaataca ttcaaatatg tatccgctca
     4981 tgagacaata accctgataa atgcttcaat aatattgaaa aaggaagagt atgagtattc
     5041 aacatttccg tgtcgccctt attccctttt ttgcggcatt ttgccttcct gtttttgctc
     5101 acccagaaac gctggtgaaa gtaaaagatg ctgaagatca gttgggtgca cgagtgggtt
     5161 acatcgaact ggatctcaac agcggtaaga tccttgagag ttttcgcccc gaagaacgtt
     5221 ttccaatgat gagcactttt aaagttctgc tatgtggcgc ggtattatcc cgtattgacg
     5281 ccgggcaaga gcaactcggt cgccgcatac actattctca gaatgacttg gttgagtact
     5341 caccagtcac agaaaagcat cttacggatg gcatgacagt aagagaatta tgcagtgctg
     5401 ccataaccat gagtgataac actgcggcca acttacttct gacaacgatc ggaggaccga
     5461 aggagctaac cgcttttttg cacaacatgg gggatcatgt aactcgcctt gatcgttggg
     5521 aaccggagct gaatgaagcc ataccaaacg acgagcgtga caccacgatg cctgtagcaa
     5581 tggcaacaac gttgcgcaaa ctattaactg gcgaactact tactctagct tcccggcaac
     5641 aattaataga ctggatggag gcggataaag ttgcaggacc acttctgcgc tcggcccttc
     5701 cggctggctg gtttattgct gataaatctg gagccggtga gcgtgggtct cgcggtatca
     5761 ttgcagcact ggggccagat ggtaagccct cccgtatcgt agttatctac acgacgggga
     5821 gtcaggcaac tatggatgaa cgaaatagac agatcgctga gataggtgcc tcactgatta
     5881 agcattggta actgtcagac caagtttact catatatact ttagattgat ttaaaacttc
     5941 atttttaatt taaaaggatc taggtgaaga tcctttttga taatctcatg accaaaatcc
     6001 cttaacgtga gttttcgttc cactgagcgt cagaccccgt agaaaagatc aaaggatctt
     6061 cttgagatcc tttttttctg cgcgtaatct gctgcttgca aacaaaaaaa ccaccgctac
     6121 cagcggtggt ttgtttgccg gatcaagagc taccaactct ttttccgaag gtaactggct
     6181 tcagcagagc gcagatacca aatactgttc ttctagtgta gccgtagtta ggccaccact
     6241 tcaagaactc tgtagcaccg cctacatacc tcgctctgct aatcctgtta ccagtggctg
     6301 ctgccagtgg cgataagtcg tgtcttaccg ggttggactc aagacgatag ttaccggata
     6361 aggcgcagcg gtcgggctga acggggggtt cgtgcacaca gcccagcttg gagcgaacga
     6421 cctacaccga actgagatac ctacagcgtg agctatgaga aagcgccacg cttcccgaag
     6481 ggagaaaggc ggacaggtat ccggtaagcg gcagggtcgg aacaggagag cgcacgaggg
     6541 agcttccagg gggaaacgcc tggtatcttt atagtcctgt cgggtttcgc cacctctgac
     6601 ttgagcgtcg atttttgtga tgctcgtcag gggggcggag cctatggaaa aacgccagca
     6661 acgcggcctt tttacggttc ctggcctttt gctggccttt tgctcacatg ttctttcctg
     6721 cgttatcccc tgattctgtg gataaccgta ttaccgcctt tgagtgagct gataccgctc
     6781 gccgcagccg aacgaccgag cgcagcgagt cagtgagcga ggaagcggaa gagcgcccaa
     6841 tacgcaaacc gcctctcccc gcgcgttggc cgattcatta atgcagctgg cacgacaggt
     6901 ttcccgactg gaaagcgggc agtgagcgca acgcaattaa tgtgagttag ctcactcatt
     6961 aggcacccca ggctttacac tttatgcttc cggctcgtat gttgtgtgga attgtgagcg
     7021 gataacaatt tcacacagga aacagctatg accatgatta cgccaagcgc gcaattaacc
     7081 ctcactaaag ggaacaaaag ctggagctgc aagcttaatg tagtcttatg caatactctt
     7141 gtagtcttgc aacatggtaa cgatgagtta gcaacatgcc ttacaaggag agaaaaagca
     7201 ccgtgcatgc cgattggtgg aagtaaggtg gtacgatcgt gccttattag gaaggcaaca
     7261 gacgggtctg acatggattg gacgaaccac tgaattgccg cattgcagag atattgtatt
     7321 taagtgccta gctcgataca taaacgggtc tctctggtta gaccagatct gagcctggga
     7381 gctctctggc taactaggga acccactgct taagcctcaa taaagcttgc cttgagtgct
     7441 tcaagtagtg tgtgcccgtc tgttgtgtga ctctggtaac tagagatccc tcagaccctt
     7501 ttagtcagtg tggaaaatct ctagcagtgg cgcccgaaca gggacttgaa agcgaaaggg
     7561 aaaccagagg agctctctcg acgcaggact cggcttgctg aagcgcgcac ggcaagaggc
     7621 gaggggcggc gactggtgag tacgccaaaa attttgacta gcggaggcta gaaggagaga
     7681 gatgggtgcg agagcgtcag tattaagcgg gggagaatta gatcgcgatg ggaaaaaatt
     7741 cggttaaggc cagggggaaa gaaaaaatat aaattaaaac atatagtatg ggcaagcagg
     7801 gagctagaac gattcgcagt taatcctggc ctgttagaaa catcagaagg ctgtagacaa
     7861 atactgggac agctacaacc atcccttcag acaggatcag aagaacttag atcattatat
     7921 aatacagtag caaccctcta ttgtgtgcat caaaggatag agataaaaga caccaaggaa
     7981 gctttagaca agatagagga agagcaaaac aaaagtaaga ccaccgcaca gcaagcggcc
     8041 gctgatcttc agacctggag gaggagatat gagggacaat tggagaagtg aattatataa
     8101 atataaagta gtaaaaattg aaccattagg agtagcaccc accaaggcaa agagaagagt
     8161 ggtgcagaga gaaaaaagag cagtgggaat aggagctttg ttccttgggt tcttgggagc
     8221 agcaggaagc actatgggcg cagcgtcaat gacgctgacg gtacaggcca gacaattatt
     8281 gtctggtata gtgcagcagc agaacaattt gctgagggct attgaggcgc aacagcatct
     8341 gttgcaactc acagtctggg gcatcaagca gctccaggca agaatcctgg ctgtggaaag
     8401 atacctaaag gatcaacagc tcctggggat ttggggttgc tctggaaaac tcatttgcac
     8461 cactgctgtg ccttggaatg ctagttggag taataaatct ctggaacaga tttggaatca
     8521 cacgacctgg atggagtggg acagagaaat taacaattac acaagcttaa tacactcctt
     8581 aattgaagaa tcgcaaaacc agcaagaaaa gaatgaacaa gaattattgg aattagataa
     8641 atgggcaagt ttgtggaatt ggtttaacat aacaaattgg ctgtggtata taaaattatt
     8701 cataatgata gtaggaggct tggtaggttt aagaatagtt tttgctgtac tttctatagt
     8761 gaatagagtt aggcagggat attcaccatt atcgtttcag acccacctcc caaccccgag
     8821 gggacccatg cattgcatct caattagtca gcaaccaggt gtggaaagtc cccaggctcc
     8881 ccagcaggca gaagtatgca aagcatgcgt ctcaattagt cagcaaccat agtcccgccc
     8941 ctaactccgc ccatcccgcc cctaactccg cccagttccg cccattctcc gccccatggc
     9001 tgactaattt tttttattta tgcagaggcc gaggccgcct cggcctctga gctattccag
     9061 aagtagtgag gaggcttttt tggaggccta ggcttttgca aaaagctttc tagaggtacc
     9121 accggagctt accatgaccg agtacaagcc cacggtgcgc ctcgccaccc gcgacgacgt
     9181 ccccagggcc gtacgcaccc tcgccgccgc gttcgccgac taccccgcca cgcgccacac
     9241 cgtcgatccg gaccgccaca tcgagcgggt caccgagctg caagaactct tcctcacgcg
     9301 cgtcgggctc gacatcggca aggtgtgggt cgcggacgac ggcgccgccg tggcggtctg
     9361 gaccacgccg gagagcgtcg aagcgggggc ggtgttcgcc gagatcggcc cgcgcatggc
     9421 cgagttgagc ggttcccggc tggccgcgca gcaacagatg gaaggcctcc tggcgccgca
     9481 ccggcccaag gagcccgcgt ggttcctggc caccgtcggc gtctcgcccg accaccaggg
     9541 caagggtctg ggcagcgccg tcgtgctccc cggagtggag gcggccgagc gcgccggggt
     9601 gcccgccttc ctggagacct ccgcgccccg caacctcccc ttctacgagc ggctcggctt
     9661 caccgtcacc gccgacgtcg aggtgcccga aggaccgcgc acctggtgca tgacccgcaa
     9721 gcccggtgcc agttaacgaa ttctagatct tgagacaaat ggcagtattc atccacaatt
     9781 ttaaaagaaa aggggggatt ggggggtaca gtgcagggga aagaatagta gacataatag
     9841 caacagacat acaaactaaa gaattacaaa aacaaattac aaaaattcaa aattttcggg
     9901 tttattacag ggacagcaga gatccacttt ggcgccggct cgaggcctgc aggtgcaaag
     9961 atggataaag ttttaaacag agaggaatct ttgcagctaa tggaccttct aggtcttgaa
    10021 aggagtggga att
//
