LOCUS       sequence-353402-        6488 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  The tag is SsrA (AANDENY). Tag is inserted after N534 of Src..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6488)
  AUTHORS   Liu B, Stone OJ, Pablo M, Herron JC, Nogueira AT, Dagliyan O, Grimm 
            JB, Lavis LD, Elston TC, Hahn KM
  TITLE     Biosensors based on peptide exposure show single molecule 
            conformations in live cells.
  JOURNAL   Cell. 2021 Oct 28;184(22):5670-5685.e23. doi: 
            10.1016/j.cell.2021.09.026. Epub 2021 Oct 11.
  PUBMED    34637702
REFERENCE   2  (bases 1 to 6488)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2021.09.026"; journalName: "Cell"; date: 
            "2021-10-28- 28"; volume: "184"; issue: "22"; pages: "5670-5685.e23"
COMMENT     Sequence Label: tagSrc-HaloTag
FEATURES             Location/Qualifiers
     source          1..6488
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     331..350
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     enhancer        605..908
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        909..1112
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     1063..1083
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     regulatory      1149..1158
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             2799..3689
                     /codon_start=1
                     /product="modified bacterial dehalogenase that forms 
                     covalent bonds with chloroalkane derivatives"
                     /label=HaloTag(R)
                     /translation="MAEIGTGFPFDPHYVEVLGERMHYVDVGPRDGTPVLFLHGNPTSS
                     YVWRNIIPHVAPTHRCIAPDLIGMGKSDKPDLGYFFDDHVRFMDAFIEALGLEEVVLVI
                     HDWGSALGFHWAKRNPERVKGIAFMEFIRPIPTWDEWPEFARETFQAFRTTDVGRKLII
                     DQNVFIEGTLPMGVVRPLTEVEMDHYREPFLNPVDREPLWRFPNELPIAGEPANIVALV
                     EEYMDWLHQSPVPKLLFWGTPGVLIPPAEAARLAKSLPNCKAVDIGPGLNLLQEDNPDL
                     IGSEIARWLSTLEISG"
     polyA_signal    3820..3941
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(3857..3876)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     3911..3930
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      complement(3948..4403)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(4085..4106)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     4297..4316
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     promoter        4430..4534
                     /gene="bla"
                     /label=AmpR promoter
     promoter        4536..4893
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     enhancer        4536..4727
                     /label=SV40 enhancer
                     /note="enhancer for the SV40 early promoter (Herr, 1993)"
     primer_bind     complement(4559..4579)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     rep_origin      4744..4879
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             4928..5722
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     CDS             4928..5722
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(4982..5001)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     5592..5611
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     complement(5910..5929)
                     /label=TK-pA-R
                     /note="Thymidine kinase polyA, reverse primer"
     polyA_signal    5954..6001
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     rep_origin      join(6330..6488,1..430)
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
ORIGIN
        1 gccgtagtta ggccaccact tcaagaactc tgtagcaccg cctacatacc tcgctctgct
       61 aatcctgtta ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg ggttggactc
      121 aagacgatag ttaccggata aggcgcagcg gtcgggctga acggggggtt cgtgcacaca
      181 gcccagcttg gagcgaacga cctacaccga actgagatac ctacagcgtg agctatgaga
      241 aagcgccacg cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg gcagggtcgg
      301 aacaggagag cgcacgaggg agcttccagg gggaaacgcc tggtatcttt atagtcctgt
      361 cgggtttcgc cacctctgac ttgagcgtcg atttttgtga tgctcgtcag gggggcggag
      421 cctatggaaa aacgccagca acgcggcctt tttacggttc ctggcctttt gctggccttt
      481 tgctcacatg ttctttcctg cgttatcccc tgattctgtg gataaccgta ttaccgccat
      541 gcattagtta ttaatagtaa tcaattacgg ggtcattagt tcatagccca tatatggagt
      601 tccgcgttac ataacttacg gtaaatggcc cgcctggctg accgcccaac gacccccgcc
      661 cattgacgtc aataatgacg tatgttccca tagtaacgcc aatagggact ttccattgac
      721 gtcaatgggt ggagtattta cggtaaactg cccacttggc agtacatcaa gtgtatcata
      781 tgccaagtac gccccctatt gacgtcaatg acggtaaatg gcccgcctgg cattatgccc
      841 agtacatgac cttatgggac tttcctactt ggcagtacat ctacgtatta gtcatcgcta
      901 ttaccatggt gatgcggttt tggcagtaca tcaatgggcg tggatagcgg tttgactcac
      961 ggggatttcc aagtctccac cccattgacg tcaatgggag tttgttttgg caccaaaatc
     1021 aacgggactt tccaaaatgt cgtaacaact ccgccccatt gacgcaaatg ggcggtaggc
     1081 gtgtacggtg ggaggtctat ataagcagag ctggtttagt gaaccgtcag atccgctagc
     1141 ttaattaagc caccatgggc agcaacaaga gcaagcccaa ggacgccagc cagcggcgcc
     1201 gcagcctgga gccctcggaa aacgtgcacg gggcaggggg cgccttcccg gcctcacaga
     1261 caccgagcaa gcccgcctcc gccgacggcc accgcgggcc cagcgccgcc ttcgtgccgc
     1321 ccgcggccga gcccaagctc ttcggaggct tcaactcctc ggacaccgtc acctccccgc
     1381 agagggcggg gcctctggca ggtggggtga ccacctttgt ggccctctat gactatgagt
     1441 cacggacaga gactgacctg tccttcaaga aaggggagcg gctgcagatt gttaacaaca
     1501 cggagggaga ctggtggctg gcacactcgc tgagcacggg acagaccggt tacatcccca
     1561 gcaactatgt ggcgccctcc gactccatcc aggctgagga gtggtacttt ggcaagatca
     1621 ctagacggga atcagagcgg ctgctgctca acgccgagaa cccgagaggg accttcctcg
     1681 tgagggagag tgagaccaca aaaggtgcct actgcctctc tgtatccgac ttcgacaatg
     1741 ccaagggcct aaatgtgaaa cactacaaga tccgcaagct ggacagcggc ggtttctaca
     1801 tcacctcccg cacccagttc aacagcctgc agcagctcgt ggcttactac tccaaacatg
     1861 ctgatggcct gtgtcaccgc ctcactaccg tatgtcccac atccaagcct cagacccagg
     1921 gattggccaa ggatgcgtgg gagatccccc gggagtccct gcggctggag gtcaagctgg
     1981 gccagggttg cttcggagag gtgtggatgg ggacctggaa cggcaccacg agggttgcca
     2041 tcaaaactct gaagccaggc accatgtccc cagaggcctt cctgcaggag gcccaagtca
     2101 tgaagaaact taggcacgag aaactggtgc agctgtatgc tgtggtgtcg gaagaaccca
     2161 tttacattgt gacagagtac atgaacaagg ggagtctgct ggactttctc aagggggaaa
     2221 cgggcaaata tttgcggcta ccccagctgg tggacatgtc tgctcagatc gcttcaggca
     2281 tggcctatgt ggagcggatg aactatgtgc accgggacct tcgagccgcc aatatcctag
     2341 taggggagaa cctggtgtgc aaagtggccg actttgggtt ggcccggctc atagaagaca
     2401 acgaatacac agcccggcaa ggtgccaaat tccccatcaa gtggaccgcc cctgaagctg
     2461 ctctgtacgg caggttcacc atcaagtcgg atgtgtggtc ctttgggatt ctgctgaccg
     2521 agctcaccac taagggaaga gtgccctatc ctgggatggt gaaccgtgag gttctggacc
     2581 aggtggagcg gggctaccgg atgccttgtc cccccgagtg ccccgagtcc ctgcatgacc
     2641 ttatgtgcca gtgctggcgg aaggagcccg aggagcggcc caccttcgag tacctgcagg
     2701 ccttcctgga agactacttt acgtccactg agccacagta ccagcccggg gagaacgctg
     2761 caaatgatga aaactaccta ggcggaccag ggaagcttat ggcagaaatc ggtactggct
     2821 ttccattcga cccccattat gtggaagtcc tgggcgagcg catgcactac gtcgatgttg
     2881 gtccgcgcga tggcacccct gtgctgttcc tgcacggtaa cccgacctcc tcctacgtgt
     2941 ggcgcaacat catcccgcat gttgcaccga cccatcgctg cattgctcca gacctgatcg
     3001 gtatgggcaa atccgacaaa ccagacctgg gttatttctt cgacgaccac gtccgcttca
     3061 tggatgcctt catcgaagcc ctgggtctgg aagaggtcgt cctggtcatt cacgactggg
     3121 gctccgctct gggtttccac tgggccaagc gcaatccaga gcgcgtcaaa ggtattgcat
     3181 ttatggagtt catccgccct atcccgacct gggacgaatg gccagaattt gcccgcgaga
     3241 ccttccaggc cttccgcacc accgacgtcg gccgcaagct gatcatcgat cagaacgttt
     3301 ttatcgaggg tacgctgccg atgggtgtcg tccgcccgct gactgaagtc gagatggacc
     3361 attaccgcga gccgttcctg aatcctgttg accgcgagcc actgtggcgc ttcccaaacg
     3421 agctgccaat cgccggtgag ccagcgaaca tcgtcgcgct ggtcgaagaa tacatggact
     3481 ggctgcacca gtcccctgtc ccgaagctgc tgttctgggg caccccaggc gttctgatcc
     3541 caccggccga agccgctcgc ctggccaaaa gcctgcctaa ctgcaaggct gtggacatcg
     3601 gcccgggtct gaatctgctg caagaagaca acccggacct gatcggcagc gagatcgcgc
     3661 gctggctgtc gacgctcgag atttccggcg aattctaagc ggccgcgact ctagatcata
     3721 atcagccata ccacatttgt agaggtttta cttgctttaa aaaacctccc acacctcccc
     3781 ctgaacctga aacataaaat gaatgcaatt gttgttgtta acttgtttat tgcagcttat
     3841 aatggttaca aataaagcaa tagcatcaca aatttcacaa ataaagcatt tttttcactg
     3901 cattctagtt gtggtttgtc caaactcatc aatgtatctt aaggcgtaaa ttgtaagcgt
     3961 taatattttg ttaaaattcg cgttaaattt ttgttaaatc agctcatttt ttaaccaata
     4021 ggccgaaatc ggcaaaatcc cttataaatc aaaagaatag accgagatag ggttgagtgt
     4081 tgttccagtt tggaacaaga gtccactatt aaagaacgtg gactccaacg tcaaagggcg
     4141 aaaaaccgtc tatcagggcg atggcccact acgtgaacca tcaccctaat caagtttttt
     4201 ggggtcgagg tgccgtaaag cactaaatcg gaaccctaaa gggagccccc gatttagagc
     4261 ttgacgggga aagccggcga acgtggcgag aaaggaaggg aagaaagcga aaggagcggg
     4321 cgctagggcg ctggcaagtg tagcggtcac gctgcgcgta accaccacac ccgccgcgct
     4381 taatgcgccg ctacagggcg cgtcaggtgg cacttttcgg ggaaatgtgc gcggaacccc
     4441 tatttgttta tttttctaaa tacattcaaa tatgtatccg ctcatgagac aataaccctg
     4501 ataaatgctt caataatatt gaaaaaggaa gagtcctgag gcggaaagaa ccagctgtgg
     4561 aatgtgtgtc agttagggtg tggaaagtcc ccaggctccc cagcaggcag aagtatgcaa
     4621 agcatgcatc tcaattagtc agcaaccagg tgtggaaagt ccccaggctc cccagcaggc
     4681 agaagtatgc aaagcatgca tctcaattag tcagcaacca tagtcccgcc cctaactccg
     4741 cccatcccgc ccctaactcc gcccagttcc gcccattctc cgccccatgg ctgactaatt
     4801 ttttttattt atgcagaggc cgaggccgcc tcggcctctg agctattcca gaagtagtga
     4861 ggaggctttt ttggaggcct aggcttttgc aaagatcgat caagagacag gatgaggatc
     4921 gtttcgcatg attgaacaag atggattgca cgcaggttct ccggccgctt gggtggagag
     4981 gctattcggc tatgactggg cacaacagac aatcggctgc tctgatgccg ccgtgttccg
     5041 gctgtcagcg caggggcgcc cggttctttt tgtcaagacc gacctgtccg gtgccctgaa
     5101 tgaactgcaa gacgaggcag cgcggctatc gtggctggcc acgacgggcg ttccttgcgc
     5161 agctgtgctc gacgttgtca ctgaagcggg aagggactgg ctgctattgg gcgaagtgcc
     5221 ggggcaggat ctcctgtcat ctcaccttgc tcctgccgag aaagtatcca tcatggctga
     5281 tgcaatgcgg cggctgcata cgcttgatcc ggctacctgc ccattcgacc accaagcgaa
     5341 acatcgcatc gagcgagcac gtactcggat ggaagccggt cttgtcgatc aggatgatct
     5401 ggacgaagag catcaggggc tcgcgccagc cgaactgttc gccaggctca aggcgagcat
     5461 gcccgacggc gaggatctcg tcgtgaccca tggcgatgcc tgcttgccga atatcatggt
     5521 ggaaaatggc cgcttttctg gattcatcga ctgtggccgg ctgggtgtgg cggaccgcta
     5581 tcaggacata gcgttggcta cccgtgatat tgctgaagag cttggcggcg aatgggctga
     5641 ccgcttcctc gtgctttacg gtatcgccgc tcccgattcg cagcgcatcg ccttctatcg
     5701 ccttcttgac gagttcttct gagcgggact ctggggttcg aaatgaccga ccaagcgacg
     5761 cccaacctgc catcacgaga tttcgattcc accgccgcct tctatgaaag gttgggcttc
     5821 ggaatcgttt tccgggacgc cggctggatg atcctccagc gcggggatct catgctggag
     5881 ttcttcgccc accctagggg gaggctaact gaaacacgga aggagacaat accggaagga
     5941 acccgcgcta tgacggcaat aaaaagacag aataaaacgc acggtgttgg gtcgtttgtt
     6001 cataaacgcg gggttcggtc ccagggctgg cactctgtcg ataccccacc gagaccccat
     6061 tggggccaat acgcccgcgt ttcttccttt tccccacccc accccccaag ttcgggtgaa
     6121 ggcccagggc tcgcagccaa cgtcggggcg gcaggccctg ccatagcctc aggttactca
     6181 tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc
     6241 ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca
     6301 gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc
     6361 tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta
     6421 ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgttctt
     6481 ctagtgta
//
