LOCUS       sequence-353403-        5958 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Note- This plasmid contains Keratin 18. Localization: Cytokeratin, 
            Excitation: 558, Emission: 605.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5958)
  TITLE     Davidson Red fluorescent proteins
REFERENCE   2  (bases 1 to 5958)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: mRuby-Keratin-17
FEATURES             Location/Qualifiers
     source          1..5958
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        1..304
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        305..508
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     459..479
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     CDS             1881..2561
                     /codon_start=1
                     /product="monomeric variant of the red fluorescent protein 
                     eqFP611 (Kredel et al., 2009)"
                     /label=mRuby
                     /translation="MNSLIKENMRMKVVLEGSVNGHQFKCTGEGEGNPYMGTQTMRIKV
                     IEGGPLPFAFDILATSFMYGSRTFIKYPKGIPDFFKQSFPEGFTWERVTRYEDGGVITV
                     MQDTSLEDGCLVYHAQVRGVNFPSNGAVMQKKTKGWEPNTEMMYPADGGLRGYTHMALK
                     VDGGGHLSCSFVTTYRSKKTVGNIKMPGIHAVDHRLERLEESDNEMFVVQREHAVAKFA
                     GLGGG"
     polyA_signal    2686..2807
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(2723..2742)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     2777..2796
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      complement(2814..3269)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(2951..2972)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     3163..3182
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     promoter        3296..3400
                     /gene="bla"
                     /label=AmpR promoter
     promoter        3402..3759
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     enhancer        3402..3593
                     /label=SV40 enhancer
                     /note="enhancer for the SV40 early promoter (Herr, 1993)"
     primer_bind     complement(3425..3445)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     rep_origin      3610..3745
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             3794..4588
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     CDS             3794..4588
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(3848..3867)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     4458..4477
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     complement(4776..4795)
                     /label=TK-pA-R
                     /note="Thymidine kinase polyA, reverse primer"
     polyA_signal    4820..4867
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     rep_origin      5196..5784
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     5685..5704
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc cccgcccatt
       61 gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc attgacgtca
      121 atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt atcatatgcc
      181 aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt atgcccagta
      241 catgacctta tgggactttc ctacttggca gtacatctac gtattagtca tcgctattac
      301 catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg actcacgggg
      361 atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg
      421 ggacttttca aaatgtcgta acaactccgc cccattgacg caaatgggcg gtaggcgtgt
      481 acggtgggag gtctatataa gcagagctgg tttagtgaac cgtcagatcc gctagcacca
      541 tgagcttcac cactcgctcc accttctcca ccaactaccg gtccctgggc tctgtccagg
      601 cgcccagcta cggcgcccgg ccggtcagca gcgcggccag cgtctatgca ggcgctgggg
      661 gctctggttc ccggatctcc gtgtcccgct ccaccagctt caggggcggc atggggtccg
      721 ggggcctggc caccgggata gccgggggtc tggcaggaat gggaggcatc cagaacgaga
      781 aggagaccat gcaaagcctg aacgaccgcc tggcctctta cctggacaga gtgaggagcc
      841 tggagaccga gaaccggagg ctggagagca aaatccggga gcacttggag aagaagggac
      901 cccaggtcag agactggagc cattacttca agatcatcga ggacctgagg gctcagatct
      961 tcgcaaatac tgtggacaat gcccgcatcg ttctgcagat tgacaatgcc cgtcttgctg
     1021 ctgatgactt tagagtcaag tatgagacag agctggccat gcgccagtct gtggagaacg
     1081 acatccatgg gctccgcaag gtcattgatg acaccaatat cacacgactg cagctggaga
     1141 cagagatcga ggctctcaag gaggagctgc tcttcatgaa gaagaaccac gaagaggaag
     1201 taaaaggcct acaagcccag attgccagct ctgggttgac cgtggaggta gatgccccca
     1261 aatctcagga cctcgccaag atcatggcag acatccgggc ccaatatgac gagctggctc
     1321 ggaagaaccg agaggagcta gacaagtact ggtctcagca gattgaggag agcaccacag
     1381 tgctcaccac acagtctgct gaggttggag ctgctgagac gacgctcaca gagctgagac
     1441 gtacagtcca gtccttggag atcgacctgg actccatgag aaatctgaag gccagcttgg
     1501 agaacagcct gagggaggtg gaggcccgct acgccctaca gatggagcag ctcaacggga
     1561 tcctgctgca ccttgagtca gagctggcac agacccgggc agagggacag cgccaggccc
     1621 aggagtatga ggccctgctg aacatcaagg tcaagctgga ggctgagatc gccacctacc
     1681 gccgcctgct ggaagatggc gaggacttta atcttggtga tgccttggac agcagcaact
     1741 ccatgcaaac catccaaaag accaccaccc gccggatagt ggatggcaaa gtggtgtctg
     1801 agaccaatga caccaaagtt ctgaggcatc gaattctgca gtcgacggta ccgcgggccc
     1861 gggatccacc ggtcgccacc atgaacagcc tgatcaaaga aaacatgcgg atgaaggtgg
     1921 tgctggaagg cagcgtgaac ggccaccagt tcaagtgcac cggcgagggc gagggcaacc
     1981 cctacatggg cacccagacc atgcggatca aagtgatcga gggcggacct ctgcccttcg
     2041 ccttcgacat cctggccaca tccttcatgt acggcagccg gaccttcatc aagtacccca
     2101 agggcatccc cgatttcttc aagcagagct tccccgaggg cttcacctgg gagagagtga
     2161 ccagatacga ggacggcggc gtgatcaccg tgatgcagga caccagcctg gaagatggct
     2221 gcctggtgta ccatgcccag gtcaggggcg tgaattttcc cagcaacggc gccgtgatgc
     2281 agaagaaaac caagggctgg gagcccaaca ccgagatgat gtaccccgct gacggcggac
     2341 tgagaggcta cacccacatg gccctgaagg tggacggcgg agggcacctg agctgcagct
     2401 tcgtgaccac ctaccgatcc aagaaaaccg tgggcaacat caagatgccc ggcatccacg
     2461 ccgtggacca ccggctggaa aggctggaag agtccgacaa cgagatgttc gtggtgcagc
     2521 gggagcacgc cgtggccaag ttcgccggcc tgggcggagg gtaagcggcc gcgactctag
     2581 atcataatca gccataccac atttgtagag gttttacttg ctttaaaaaa cctcccacac
     2641 ctccccctga acctgaaaca taaaatgaat gcaattgttg ttgttaactt gtttattgca
     2701 gcttataatg gttacaaata aagcaatagc atcacaaatt tcacaaataa agcatttttt
     2761 tcactgcatt ctagttgtgg tttgtccaaa ctcatcaatg tatcttaagg cgtaaattgt
     2821 aagcgttaat attttgttaa aattcgcgtt aaatttttgt taaatcagct cattttttaa
     2881 ccaataggcc gaaatcggca aaatccctta taaatcaaaa gaatagaccg agatagggtt
     2941 gagtgttgtt ccagtttgga acaagagtcc actattaaag aacgtggact ccaacgtcaa
     3001 agggcgaaaa accgtctatc agggcgatgg cccactacgt gaaccatcac cctaatcaag
     3061 ttttttgggg tcgaggtgcc gtaaagcact aaatcggaac cctaaaggga gcccccgatt
     3121 tagagcttga cggggaaagc cggcgaacgt ggcgagaaag gaagggaaga aagcgaaagg
     3181 agcgggcgct agggcgctgg caagtgtagc ggtcacgctg cgcgtaacca ccacacccgc
     3241 cgcgcttaat gcgccgctac agggcgcgtc aggtggcact tttcggggaa atgtgcgcgg
     3301 aacccctatt tgtttatttt tctaaataca ttcaaatatg tatccgctca tgagacaata
     3361 accctgataa atgcttcaat aatattgaaa aaggaagagt cctgaggcgg aaagaaccag
     3421 ctgtggaatg tgtgtcagtt agggtgtgga aagtccccag gctccccagc aggcagaagt
     3481 atgcaaagca tgcatctcaa ttagtcagca accaggtgtg gaaagtcccc aggctcccca
     3541 gcaggcagaa gtatgcaaag catgcatctc aattagtcag caaccatagt cccgccccta
     3601 actccgccca tcccgcccct aactccgccc agttccgccc attctccgcc ccatggctga
     3661 ctaatttttt ttatttatgc agaggccgag gccgcctcgg cctctgagct attccagaag
     3721 tagtgaggag gcttttttgg aggcctaggc ttttgcaaag atcgatcaag agacaggatg
     3781 aggatcgttt cgcatgattg aacaagatgg attgcacgca ggttctccgg ccgcttgggt
     3841 ggagaggcta ttcggctatg actgggcaca acagacaatc ggctgctctg atgccgccgt
     3901 gttccggctg tcagcgcagg ggcgcccggt tctttttgtc aagaccgacc tgtccggtgc
     3961 cctgaatgaa ctgcaagacg aggcagcgcg gctatcgtgg ctggccacga cgggcgttcc
     4021 ttgcgcagct gtgctcgacg ttgtcactga agcgggaagg gactggctgc tattgggcga
     4081 agtgccgggg caggatctcc tgtcatctca ccttgctcct gccgagaaag tatccatcat
     4141 ggctgatgca atgcggcggc tgcatacgct tgatccggct acctgcccat tcgaccacca
     4201 agcgaaacat cgcatcgagc gagcacgtac tcggatggaa gccggtcttg tcgatcagga
     4261 tgatctggac gaagagcatc aggggctcgc gccagccgaa ctgttcgcca ggctcaaggc
     4321 gagcatgccc gacggcgagg atctcgtcgt gacccatggc gatgcctgct tgccgaatat
     4381 catggtggaa aatggccgct tttctggatt catcgactgt ggccggctgg gtgtggcgga
     4441 ccgctatcag gacatagcgt tggctacccg tgatattgct gaagagcttg gcggcgaatg
     4501 ggctgaccgc ttcctcgtgc tttacggtat cgccgctccc gattcgcagc gcatcgcctt
     4561 ctatcgcctt cttgacgagt tcttctgagc gggactctgg ggttcgaaat gaccgaccaa
     4621 gcgacgccca acctgccatc acgagatttc gattccaccg ccgccttcta tgaaaggttg
     4681 ggcttcggaa tcgttttccg ggacgccggc tggatgatcc tccagcgcgg ggatctcatg
     4741 ctggagttct tcgcccaccc tagggggagg ctaactgaaa cacggaagga gacaataccg
     4801 gaaggaaccc gcgctatgac ggcaataaaa agacagaata aaacgcacgg tgttgggtcg
     4861 tttgttcata aacgcggggt tcggtcccag ggctggcact ctgtcgatac cccaccgaga
     4921 ccccattggg gccaatacgc ccgcgtttct tccttttccc caccccaccc cccaagttcg
     4981 ggtgaaggcc cagggctcgc agccaacgtc ggggcggcag gccctgccat agcctcaggt
     5041 tactcatata tactttagat tgatttaaaa cttcattttt aatttaaaag gatctaggtg
     5101 aagatccttt ttgataatct catgaccaaa atcccttaac gtgagttttc gttccactga
     5161 gcgtcagacc ccgtagaaaa gatcaaagga tcttcttgag atcctttttt tctgcgcgta
     5221 atctgctgct tgcaaacaaa aaaaccaccg ctaccagcgg tggtttgttt gccggatcaa
     5281 gagctaccaa ctctttttcc gaaggtaact ggcttcagca gagcgcagat accaaatact
     5341 gttcttctag tgtagccgta gttaggccac cacttcaaga actctgtagc accgcctaca
     5401 tacctcgctc tgctaatcct gttaccagtg gctgctgcca gtggcgataa gtcgtgtctt
     5461 accgggttgg actcaagacg atagttaccg gataaggcgc agcggtcggg ctgaacgggg
     5521 ggttcgtgca cacagcccag cttggagcga acgacctaca ccgaactgag atacctacag
     5581 cgtgagctat gagaaagcgc cacgcttccc gaagggagaa aggcggacag gtatccggta
     5641 agcggcaggg tcggaacagg agagcgcacg agggagcttc cagggggaaa cgcctggtat
     5701 ctttatagtc ctgtcgggtt tcgccacctc tgacttgagc gtcgattttt gtgatgctcg
     5761 tcaggggggc ggagcctatg gaaaaacgcc agcaacgcgg cctttttacg gttcctggcc
     5821 ttttgctggc cttttgctca catgttcttt cctgcgttat cccctgattc tgtggataac
     5881 cgtattaccg ccatgcatta gttattaata gtaatcaatt acggggtcat tagttcatag
     5941 cccatatatg gagttccg
//
