LOCUS       sequence-353412-       10483 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10483)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 10483)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF0551
FEATURES             Location/Qualifiers
     source          1..10483
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        1..1179
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          232..1170
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     1127..1147
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     misc_feature    3421..4009
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(3474..3494)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(3892..3903)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             4081..4314
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    4392..4513
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(4429..4448)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     4483..4502
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      4553..4688
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(4708..4727)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(4709..4727)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(4737..4754)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(4737..4753)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(4746..4768)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      4895..5350
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(4982..5001)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     5192..5213
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        5376..5480
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(5481..5549,5550..6341)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(5699..5718)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      6512..7100
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     7001..7020
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     7254..7271
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    7388..7409
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(7424..7429,7430..7447,7448..7454)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7462..7478
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     7467..7489
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     7486..7502
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     7486..7502
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     7521..7541
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        7523..7541
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        7569..7795
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             7796..7976
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    8023..8148
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    8641..8874
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             9059..9103
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     promoter        9334..9544
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     promoter        9356..9552
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     rep_origin      9403..9538
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             9584..10186
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGAS"
     primer_bind     complement(9584..9603)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     10080..10100
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     misc_feature    10229..10346
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
ORIGIN
        1 ggctccggtg cccgtcagtg ggcagagcgc acatcgccca cagtccccga gaagttgggg
       61 ggaggggtcg gcaattgaac cggtgcctag agaaggtggc gcggggtaaa ctgggaaagt
      121 gatgtcgtgt actggctccg cctttttccc gagggtgggg gagaaccgta tataagtgca
      181 gtagtcgccg tgaacgttct ttttcgcaac gggtttgccg ccagaacaca ggtaagtgcc
      241 gtgtgtggtt cccgcgggcc tggcctcttt acgggttatg gcccttgcgt gccttgaatt
      301 acttccacct ggctgcagta cgtgattctt gatcccgagc ttcgggttgg aagtgggtgg
      361 gagagttcga ggccttgcgc ttaaggagcc ccttcgcctc gtgcttgagt tgaggcctgg
      421 cctgggcgct ggggccgccg cgtgcgaatc tggtggcacc ttcgcgcctg tctcgctgct
      481 ttcgataagt ctctagccat ttaaaatttt tgatgacctg ctgcgacgct ttttttctgg
      541 caagatagtc ttgtaaatgc gggccaagat ctgcacactg gtatttcggt ttttggggcc
      601 gcgggcggcg acggggcccg tgcgtcccag cgcacatgtt cggcgaggcg gggcctgcga
      661 gcgcggccac cgagaatcgg acgggggtag tctcaagctg gccggcctgc tctggtgcct
      721 ggcctcgcgc cgccgtgtat cgccccgccc tgggcggcaa ggctggcccg gtcggcacca
      781 gttgcgtgag cggaaagatg gccgcttccc ggccctgctg cagggagctc aaaatggagg
      841 acgcggcgct cgggagagcg ggcgggtgag tcacccacac aaaggaaaag ggcctttccg
      901 tcctcagccg tcgcttcatg tgactccacg gagtaccggg cgccgtccag gcacctcgat
      961 tagttctcga gcttttggag tacgtcgtct ttaggttggg gggaggggtt ttatgcgatg
     1021 gagtttcccc acactgagtg ggtggagact gaagttaggc cagcttggca cttgatgtaa
     1081 ttctccttgg aatttgccct ttttgagttt ggatcttggt tcattctcaa gcctcagaca
     1141 gtggttcaaa gtttttttct tccatttcag gtgtcgtgag gctagcaccg ccaccatgtt
     1201 acgtgaggaa gcagctcaga agaggaaagg aaaggagcca ggcatggctc ttcctcaggg
     1261 acgcttgact ttccgggatg tggctataga attctcattg gcagagtgga aatgcctgaa
     1321 cccttcacag agggctttgt acagggaagt gatgttggag aactacagga acctggaggc
     1381 tgtggatatc tcttccaaat gcatgatgaa ggaggtcttg tcaacagggc aaggcaatac
     1441 agaagtgatc cacacaggga cattgcaaag acatgaaagt catcacattg gagatttttg
     1501 cttccaggaa attgagaaag aaattcatga cattgagttt cagtgtcaag aagatgaaag
     1561 aaatggcctt gaagcaccca tgacaaaaat aaaaaagttg actggtagca cagaccaaca
     1621 tgatcacagg catgctggaa acaagcctat taaagatcag cttggatcaa gcttttattc
     1681 acatctgcct gaactccaca tatttcagat caaaggtgaa attggtaatc aacttgagaa
     1741 gtctactaat gatgctccct cagtttcaac attccaaaga atttcctgta ggccccaaac
     1801 ccagatttct aataactatg ggaataatcc cctgaattct tcattactcc cacaaaaaca
     1861 ggaagtacac atgagagaaa aatctttcca atgtaataag agtggcaaag cctttaattg
     1921 tagctcactc ttaaggaaac accagatacc ccatttagga gacaaacaat ataaatgtga
     1981 tgtatgtggc aagctcttta atcacgagca ataccttgca tgccatgata gatgtcacac
     2041 tgttgagaaa ccttacaagt gtaaagagtg tggcaagacc ttcagtcagg agtcatccct
     2101 tacctgccat cgtagacttc atactggagt aaaacgttac aattgtaatg agtgtggcaa
     2161 gatctttggt caaaattcag cccttctaat tgataaggca attgatactg gagaaaatcc
     2221 ttacaagtgt aatgaatgtg acaaggcttt taatcaacaa tcacaacttt cacatcatag
     2281 aattcatact ggagagaaac cttacaaatg tgaagaatgt gacaaagttt tcagtcggaa
     2341 atcaaccatt gagacacata agagaattca tactggagag aaaccataca gatgtaaggt
     2401 ttgtgacaca gctttcacgt ggcattcaca gctggctcga catagaagaa ttcatactgc
     2461 aaagaaaact tataaatgta atgagtgtgg caagaccttc agtcacaagt catcccttgt
     2521 atgccatcat agacttcatg gtggagagaa atcttacaaa tgtaaggttt gtgacaaggc
     2581 ttttgtgtgg agttcacaac tggcaaaaca tactagaatt gactgtggag aaaaacctta
     2641 caagtgtaat gaatgtggga agacctttgg tcaaaattca gatcttttaa ttcataagtc
     2701 aattcatact ggggagcaac cttacaaatg tgatgaatgt gaaaaggttt tcagtcgtaa
     2761 atcaagcctt gagacacata agataggtca tactggagag aaaccataca aatgtaaggt
     2821 ttgtgacaag gcttttgcgt gtcattccta tctggcaaaa catactagaa ttcatagtgg
     2881 agagaaacct tacaagtgta atgagtgcag caagaccttc agtcacaggt cataccttgt
     2941 atgccatcat agagttcata gtggtgagaa accttacaag tgtaatgagt gcagcaagac
     3001 cttcagtcgc aggtcatccc ttcattgcca tcgtagactt catagtggtg agaaacctta
     3061 caagtgtaat gagtgtggca ataccttccg tcactgctca tcccttatat accatcgtag
     3121 acttcatact ggagagaaat cttacaaatg tacaatttgt gacaaggctt tcgtgcgtaa
     3181 ttcactcctg tcaagacata ccagaattca cactgcagag aaaccttaca agtgtaatga
     3241 atgtgggaag gcttttaatc aacaatcaca cctttcacgt catcatagaa ttcatactgg
     3301 agagaaacct tagtaatgaa ctagtccgta acttgaaagt atttcgattt cttggcttta
     3361 tatatcttgt ggaaaggacg agccgcgggc gggccagtga gtcagacgcg ttaagtcgac
     3421 aatcaacctc tggattacaa aatttgtgaa agattgactg gtattcttaa ctatgttgct
     3481 ccttttacgc tatgtggata cgctgcttta atgcctttgt atcatgctat tgcttcccgt
     3541 atggctttca ttttctcctc cttgtataaa tcctggttgc tgtctcttta tgaggagttg
     3601 tggcccgttg tcaggcaacg tggcgtggtg tgcactgtgt ttgctgacgc aacccccact
     3661 ggttggggca ttgccaccac ctgtcagctc ctttccggga ctttcgcttt ccccctccct
     3721 attgccacgg cggaactcat cgccgcctgc cttgcccgct gctggacagg ggctcggctg
     3781 ttgggcactg acaattccgt ggtgttgtcg gggaaatcat cgtcctttcc ttggctgctc
     3841 gcctgtgttg ccacctggat tctgcgcggg acgtccttct gctacgtccc ttcggccctc
     3901 aatccagcgg accttccttc ccgcggcctg ctgccggctc tgcggcctct tccgcgtctt
     3961 cgccttcgcc ctcagacgag tcggatctcc ctttgggccg cctccccgcg tcgactttaa
     4021 gaccaatgac ttacaaggca gctgtagatc ttagccactt tttaaaagaa aaggggggac
     4081 tggaagggct aattcactcc caacgaagac aagatctgct ttttgcttgt actgggtctc
     4141 tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta
     4201 agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact
     4261 ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtacgt
     4321 atagtagttc atgtcatctt attattcagt atttataact tgcaaagaaa tgaatatcag
     4381 agagtgagag gaacttgttt attgcagctt ataatggtta caaataaagc aatagcatca
     4441 caaatttcac aaataaagca tttttttcac tgcattctag ttgtggtttg tccaaactca
     4501 tcaatgtatc ttatcatgtc tggctctagc tatcccgccc ctaactccgc ccatcccgcc
     4561 cctaactccg cccagttccg cccattctcc gccccatggc tgactaattt tttttattta
     4621 tgcagaggcc gaggccgcct cggcctctga gctattccag aagtagtgag gaggcttttt
     4681 tggaggccta gggacgtacc caattcgccc tatagtgagt cgtattacgc gcgctcactg
     4741 gccgtcgttt tacaacgtcg tgactgggaa aaccctggcg ttacccaact taatcgcctt
     4801 gcagcacatc cccctttcgc cagctggcgt aatagcgaag aggcccgcac cgatcgccct
     4861 tcccaacagt tgcgcagcct gaatggcgaa tgggacgcgc cctgtagcgg cgcattaagc
     4921 gcggcgggtg tggtggttac gcgcagcgtg accgctacac ttgccagcgc cctagcgccc
     4981 gctcctttcg ctttcttccc ttcctttctc gccacgttcg ccggctttcc ccgtcaagct
     5041 ctaaatcggg ggctcccttt agggttccga tttagtgctt tacggcacct cgaccccaaa
     5101 aaacttgatt agggtgatgg ttcacgtagt gggccatcgc cctgatagac ggtttttcgc
     5161 cctttgacgt tggagtccac gttctttaat agtggactct tgttccaaac tggaacaaca
     5221 ctcaacccta tctcggtcta ttcttttgat ttataaggga ttttgccgat ttcggcctat
     5281 tggttaaaaa atgagctgat ttaacaaaaa tttaacgcga attttaacaa aatattaacg
     5341 cttacaattt aggtggcact tttcggggaa atgtgcgcgg aacccctatt tgtttatttt
     5401 tctaaataca ttcaaatatg tatccgctca tgagacaata accctgataa atgcttcaat
     5461 aatattgaaa aaggaagagt atgagtattc aacatttccg tgtcgccctt attccctttt
     5521 ttgcggcatt ttgccttcct gtttttgctc acccagaaac gctggtgaaa gtaaaagatg
     5581 ctgaagatca gttgggtgca cgagtgggtt acatcgaact ggatctcaac agcggtaaga
     5641 tccttgagag ttttcgcccc gaagaacgtt ttccaatgat gagcactttt aaagttctgc
     5701 tatgtggcgc ggtattatcc cgtattgacg ccgggcaaga gcaactcggt cgccgcatac
     5761 actattctca gaatgacttg gttgagtact caccagtcac agaaaagcat cttacggatg
     5821 gcatgacagt aagagaatta tgcagtgctg ccataaccat gagtgataac actgcggcca
     5881 acttacttct gacaacgatc ggaggaccga aggagctaac cgcttttttg cacaacatgg
     5941 gggatcatgt aactcgcctt gatcgttggg aaccggagct gaatgaagcc ataccaaacg
     6001 acgagcgtga caccacgatg cctgtagcaa tggcaacaac gttgcgcaaa ctattaactg
     6061 gcgaactact tactctagct tcccggcaac aattaataga ctggatggag gcggataaag
     6121 ttgcaggacc acttctgcgc tcggcccttc cggctggctg gtttattgct gataaatctg
     6181 gagccggtga gcgtgggtct cgcggtatca ttgcagcact ggggccagat ggtaagccct
     6241 cccgtatcgt agttatctac acgacgggga gtcaggcaac tatggatgaa cgaaatagac
     6301 agatcgctga gataggtgcc tcactgatta agcattggta actgtcagac caagtttact
     6361 catatatact ttagattgat ttaaaacttc atttttaatt taaaaggatc taggtgaaga
     6421 tcctttttga taatctcatg accaaaatcc cttaacgtga gttttcgttc cactgagcgt
     6481 cagaccccgt agaaaagatc aaaggatctt cttgagatcc tttttttctg cgcgtaatct
     6541 gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg gatcaagagc
     6601 taccaactct ttttccgaag gtaactggct tcagcagagc gcagatacca aatactgttc
     6661 ttctagtgta gccgtagtta ggccaccact tcaagaactc tgtagcaccg cctacatacc
     6721 tcgctctgct aatcctgtta ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg
     6781 ggttggactc aagacgatag ttaccggata aggcgcagcg gtcgggctga acggggggtt
     6841 cgtgcacaca gcccagcttg gagcgaacga cctacaccga actgagatac ctacagcgtg
     6901 agctatgaga aagcgccacg cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg
     6961 gcagggtcgg aacaggagag cgcacgaggg agcttccagg gggaaacgcc tggtatcttt
     7021 atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg atttttgtga tgctcgtcag
     7081 gggggcggag cctatggaaa aacgccagca acgcggcctt tttacggttc ctggcctttt
     7141 gctggccttt tgctcacatg ttctttcctg cgttatcccc tgattctgtg gataaccgta
     7201 ttaccgcctt tgagtgagct gataccgctc gccgcagccg aacgaccgag cgcagcgagt
     7261 cagtgagcga ggaagcggaa gagcgcccaa tacgcaaacc gcctctcccc gcgcgttggc
     7321 cgattcatta atgcagctgg cacgacaggt ttcccgactg gaaagcgggc agtgagcgca
     7381 acgcaattaa tgtgagttag ctcactcatt aggcacccca ggctttacac tttatgcttc
     7441 cggctcgtat gttgtgtgga attgtgagcg gataacaatt tcacacagga aacagctatg
     7501 accatgatta cgccaagcgc gcaattaacc ctcactaaag ggaacaaaag ctggagctgc
     7561 aagcttaatg tagtcttatg caatactctt gtagtcttgc aacatggtaa cgatgagtta
     7621 gcaacatgcc ttacaaggag agaaaaagca ccgtgcatgc cgattggtgg aagtaaggtg
     7681 gtacgatcgt gccttattag gaaggcaaca gacgggtctg acatggattg gacgaaccac
     7741 tgaattgccg cattgcagag atattgtatt taagtgccta gctcgataca taaacgggtc
     7801 tctctggtta gaccagatct gagcctggga gctctctggc taactaggga acccactgct
     7861 taagcctcaa taaagcttgc cttgagtgct tcaagtagtg tgtgcccgtc tgttgtgtga
     7921 ctctggtaac tagagatccc tcagaccctt ttagtcagtg tggaaaatct ctagcagtgg
     7981 cgcccgaaca gggacttgaa agcgaaaggg aaaccagagg agctctctcg acgcaggact
     8041 cggcttgctg aagcgcgcac ggcaagaggc gaggggcggc gactggtgag tacgccaaaa
     8101 attttgacta gcggaggcta gaaggagaga gatgggtgcg agagcgtcag tattaagcgg
     8161 gggagaatta gatcgcgatg ggaaaaaatt cggttaaggc cagggggaaa gaaaaaatat
     8221 aaattaaaac atatagtatg ggcaagcagg gagctagaac gattcgcagt taatcctggc
     8281 ctgttagaaa catcagaagg ctgtagacaa atactgggac agctacaacc atcccttcag
     8341 acaggatcag aagaacttag atcattatat aatacagtag caaccctcta ttgtgtgcat
     8401 caaaggatag agataaaaga caccaaggaa gctttagaca agatagagga agagcaaaac
     8461 aaaagtaaga ccaccgcaca gcaagcggcc gctgatcttc agacctggag gaggagatat
     8521 gagggacaat tggagaagtg aattatataa atataaagta gtaaaaattg aaccattagg
     8581 agtagcaccc accaaggcaa agagaagagt ggtgcagaga gaaaaaagag cagtgggaat
     8641 aggagctttg ttccttgggt tcttgggagc agcaggaagc actatgggcg cagcgtcaat
     8701 gacgctgacg gtacaggcca gacaattatt gtctggtata gtgcagcagc agaacaattt
     8761 gctgagggct attgaggcgc aacagcatct gttgcaactc acagtctggg gcatcaagca
     8821 gctccaggca agaatcctgg ctgtggaaag atacctaaag gatcaacagc tcctggggat
     8881 ttggggttgc tctggaaaac tcatttgcac cactgctgtg ccttggaatg ctagttggag
     8941 taataaatct ctggaacaga tttggaatca cacgacctgg atggagtggg acagagaaat
     9001 taacaattac acaagcttaa tacactcctt aattgaagaa tcgcaaaacc agcaagaaaa
     9061 gaatgaacaa gaattattgg aattagataa atgggcaagt ttgtggaatt ggtttaacat
     9121 aacaaattgg ctgtggtata taaaattatt cataatgata gtaggaggct tggtaggttt
     9181 aagaatagtt tttgctgtac tttctatagt gaatagagtt aggcagggat attcaccatt
     9241 atcgtttcag acccacctcc caaccccgag gggacccatg cattgcatct caattagtca
     9301 gcaaccaggt gtggaaagtc cccaggctcc ccagcaggca gaagtatgca aagcatgcgt
     9361 ctcaattagt cagcaaccat agtcccgccc ctaactccgc ccatcccgcc cctaactccg
     9421 cccagttccg cccattctcc gccccatggc tgactaattt tttttattta tgcagaggcc
     9481 gaggccgcct cggcctctga gctattccag aagtagtgag gaggcttttt tggaggccta
     9541 ggcttttgca aaaagctttc tagaggtacc accggagctt accatgaccg agtacaagcc
     9601 cacggtgcgc ctcgccaccc gcgacgacgt ccccagggcc gtacgcaccc tcgccgccgc
     9661 gttcgccgac taccccgcca cgcgccacac cgtcgatccg gaccgccaca tcgagcgggt
     9721 caccgagctg caagaactct tcctcacgcg cgtcgggctc gacatcggca aggtgtgggt
     9781 cgcggacgac ggcgccgccg tggcggtctg gaccacgccg gagagcgtcg aagcgggggc
     9841 ggtgttcgcc gagatcggcc cgcgcatggc cgagttgagc ggttcccggc tggccgcgca
     9901 gcaacagatg gaaggcctcc tggcgccgca ccggcccaag gagcccgcgt ggttcctggc
     9961 caccgtcggc gtctcgcccg accaccaggg caagggtctg ggcagcgccg tcgtgctccc
    10021 cggagtggag gcggccgagc gcgccggggt gcccgccttc ctggagacct ccgcgccccg
    10081 caacctcccc ttctacgagc ggctcggctt caccgtcacc gccgacgtcg aggtgcccga
    10141 aggaccgcgc acctggtgca tgacccgcaa gcccggtgcc agttaacgaa ttctagatct
    10201 tgagacaaat ggcagtattc atccacaatt ttaaaagaaa aggggggatt ggggggtaca
    10261 gtgcagggga aagaatagta gacataatag caacagacat acaaactaaa gaattacaaa
    10321 aacaaattac aaaaattcaa aattttcggg tttattacag ggacagcaga gatccacttt
    10381 ggcgccggct cgaggcctgc aggtgcaaag atggataaag ttttaaacag agaggaatct
    10441 ttgcagctaa tggaccttct aggtcttgaa aggagtggga att
//
