LOCUS       sequence-353413-        4022 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Insect cell expression of beta-actin.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4022)
  AUTHORS   Wieczorek M, Ti SC, Urnavicius L, Molloy KR, Aher A, Chait BT, 
            Kapoor TM
  TITLE     Biochemical reconstitutions reveal principles of human gamma-TuRC 
            assembly and function.
  JOURNAL   J Cell Biol. 2021 Mar 1;220(3). pii: 211719. doi: 
            10.1083/jcb.202009146.
  PUBMED    33496729
REFERENCE   2  (bases 1 to 4022)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Cell 
            Biol."; date: "2021-03-1"; volume: "220(3). pii: 211719. doi"; 
            pages: " 10.1083/jcb.202009146"
COMMENT     Sequence Label: pACEBac1-beta-actin
FEATURES             Location/Qualifiers
     source          1..4022
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      190..778
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     679..698
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     mobile_element  913..1136
                     /mobile_element_type="transposon:Tn7"
                     /label=Tn7R
                     /note="mini-Tn7 element (right end of the Tn7 transposon)"
     CDS             complement(1203..1736)
                     /codon_start=1
                     /gene="aacC1"
                     /product="gentamycin acetyltransferase"
                     /label=GmR
                     /note="confers resistance to gentamycin"
                     /translation="MLRSSNDVTQQGSRPKTKLGGSSMGIIRTCRLGPDQVKSMRAALD
                     LFGREFGDVATYSQHQPDSDYLGNLLRSKTFIALAAFDQEAVVGALAAYVLPKFEQPRS
                     EIYIYDLAVSGEHRRQGIATALINLLKHEANALGAYVIYVQADYGDDPAVALYTKLGIR
                     EEVMHFDIDPSTAT"
     promoter        complement(join(1925..1930,1931..1947,1948..1953))
                     /gene="intI1 (promoter lies within the coding sequence)"
                     /label=Pc promoter
                     /note="class 1 integron promoter"
     protein_bind    complement(2024..2057)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     promoter        2134..2225
                     /gene="polh from Autographa californica"
                     /label=polyhedrin promoter
                     /note="promoter for the baculovirus polyhedrin gene"
     primer_bind     2150..2167
                     /label=Polyhedrin-F
                     /note="Polyhedrin promoter, forward primer"
     polyA_signal    3602..3736
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(3639..3658)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     3693..3712
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     mobile_element  3857..4022
                     /mobile_element_type="transposon:Tn7"
                     /label=Tn7L
                     /note="mini-Tn7 element (left end of the Tn7 transposon)"
ORIGIN
        1 accggttgac ttgggtcaac tgtcagacca agtttactca tatatacttt agattgattt
       61 aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac
      121 caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa
      181 aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc
      241 accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt
      301 aactggcttc agcagagcgc agataccaaa tactgttctt ctagtgtagc cgtagttagg
      361 ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc
      421 agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt
      481 accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga
      541 gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct
      601 tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg
      661 cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca
      721 cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa
      781 cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt
      841 ctttcctgcg ttatcccctg attgacttgg gtcgctcttc ctgtggatgc gcagatgccc
      901 tgcgtaagcg ggtgtgggcg gacaataaag tcttaaactg aacaaaatag atctaaacta
      961 tgacaataaa gtcttaaact agacagaata gttgtaaact gaaatcagtc cagttatgct
     1021 gtgaaaaagc atactggact tttgttatgg ctaaagcaaa ctcttcattt tctgaagtgc
     1081 aaattgcccg tcgtattaaa gaggggcgtg gccaagggca tgtaaagact atattcgcgg
     1141 cgttgtgaca atttaccgaa caactccgcg gccgggaagc cgatctcggc ttgaacgaat
     1201 tgttaggtgg cggtacttgg gtcgatatca aagtgcatca cttcttcccg tatgcccaac
     1261 tttgtataga gagccactgc gggatcgtca ccgtaatctg cttgcacgta gatcacataa
     1321 gcaccaagcg cgttggcctc atgcttgagg agattgatga gcgcggtggc aatgccctgc
     1381 ctccggtgct cgccggagac tgcgagatca tagatataga tctcactacg cggctgctca
     1441 aacttgggca gaacgtaagc cgcgagagcg ccaacaaccg cttcttggtc gaaggcagca
     1501 agcgcgatga atgtcttact acggagcaag ttcccgaggt aatcggagtc cggctgatgt
     1561 tgggagtagg tggctacgtc tccgaactca cgaccgaaaa gatcaagagc agcccgcatg
     1621 gatttgactt ggtcagggcc gagcctacat gtgcgaatga tgcccatact tgagccacct
     1681 aactttgttt tagggcgact gccctgctgc gtaacatcgt tgctgctgcg taacatcgtt
     1741 gctgctccat aacatcaaac atcgacccac ggcgtaacgc gcttgctgct tggatgcccg
     1801 aggcatagac tgtacaaaaa aacagtcata acaagccatg aaaaccgcca ctgcgccgtt
     1861 accaccgctg cgttcggtca aggttctgga ccagttgcgt gagcgcatac gctacttgca
     1921 ttacagttta cgaaccgaac aggcttatgt caactgggtt cgtgccttca tccgtttcca
     1981 cggtgtgcgt cacccggcaa ccttgggcag cagcgaagtc gccataactt cgtatagcat
     2041 acattatacg aagttatctg taactataac ggtcctaagg tagcgagttt aaacactagt
     2101 atcgattcgc gacctactcc ggaatattaa tagatcatgg agataattaa aatgataacc
     2161 atctcgcaaa taaataagta ttttactgtt ttcgtaacag ttttgtaata aaaaaaccta
     2221 taaatattcc ggattattca taccgtccca ccatcgggcg cggatcccgg tccgaagcgc
     2281 gcggaattca tggatgatga tatcgccgcg ctcgtcgtcg acaacggctc cggcatgtgc
     2341 aaggccggct tcgcgggcga cgatgccccc cgggccgtct tcccctccat cgtggggcgc
     2401 cccaggcacc agggcgtgat ggtgggcatg ggtcagaagg attcctatgt gggcgacgag
     2461 gcccagagca agagaggcat cctcaccctg aagtacccca tcgagcacgg catcgtcacc
     2521 aactgggacg acatggagaa aatctggcac cacaccttct acaatgagct gcgtgtggct
     2581 cccgaggagc accccgtgct gctgaccgag gcccccctga accccaaggc caaccgcgag
     2641 aagatgaccc agatcatgtt tgagaccttc aacaccccag ccatgtacgt tgctatccag
     2701 gctgtgctat ccctgtacgc ctctggccgt accactggca tcgtgatgga ctccggtgac
     2761 ggggtcaccc acactgtgcc catctacgag gggtatgccc tcccccatgc catcctgcgt
     2821 ctggacctgg ctggccggga cctgactgac tacctcatga agatcctcac cgagcgcggc
     2881 tacagcttca ccaccacggc cgagcgggaa atcgtgcgtg acattaagga gaagctgtgc
     2941 tacgtcgccc tggacttcga gcaagagatg gccacggctg cttccagctc ctccctggag
     3001 aagagctacg agctgcctga cggccaggtc atcaccattg gcaatgagcg gttccgctgc
     3061 cctgaggcac tcttccagcc ttccttcctg ggcatggagt cctgtggcat ccacgaaact
     3121 accttcaact ccatcatgaa gtgtgacgtg gacatccgca aagacctgta cgccaacaca
     3181 gtgctgtctg gcggcaccac catgtaccct ggcattgccg acaggatgca aaaggagatc
     3241 actgccctgg cacccagcac aatgaagatc aagatcattg ctcctcctga gcgcaagtac
     3301 tccgtgtgga tcggcggctc catcctggcc tcgctgtcca ccttccagca gatgtggatc
     3361 agcaagcagg agtatgacga gtccggcccc tccatcgtcc accgcaaatg cttctaggtc
     3421 gacgagctca cttgtcgcgg ccgctttcga atctagagcc tgcagtctcg acaagcttgt
     3481 cgagaagtac tagaggatca taatcagcca taccacattt gtagaggttt tacttgcttt
     3541 aaaaaacctc ccacacctcc ccctgaacct gaaacataaa atgaatgcaa ttgttgttgt
     3601 taacttgttt attgcagctt ataatggtta caaataaagc aatagcatca caaatttcac
     3661 aaataaagca tttttttcac tgcattctag ttgtggtttg tccaaactca tcaatgtatc
     3721 ttatcatgtc tggatctgat cactgcttga gcctagaaga tccggctgct aacaaagccc
     3781 gaaaggaagc tgagttggct gctgccaccg ctgagcaata actatcataa cccctagggt
     3841 atacccatct aattggaacc agataagtga aatctagttc caaactattt tgtcattttt
     3901 aattttcgta ttagcttacg acgctacacc cagttcccat ctattttgtc actcttccct
     3961 aaataatcct taaaaactcc atttccaccc ctcccagttc ccaactattt tgtccgccca
     4021 ca
//
