LOCUS       sequence-353414-       15910 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 15910)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 15910)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF0702
FEATURES             Location/Qualifiers
     source          1..15910
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        1..1179
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          232..1170
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     1127..1147
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     misc_feature    8848..9436
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(8901..8921)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(9319..9330)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             9508..9741
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    9819..9940
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(9856..9875)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     9910..9929
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      9980..10115
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(10135..10154)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(10136..10154)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(10164..10181)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(10164..10180)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(10173..10195)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      10322..10777
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(10409..10428)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     10619..10640
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        10803..10907
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(10908..10976,10977..11768)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(11126..11145)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      11939..12527
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     12428..12447
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     12681..12698
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    12815..12836
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(12851..12856,12857..12874,12875..12881)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    12889..12905
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     12894..12916
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     12913..12929
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     12913..12929
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     12948..12968
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        12950..12968
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        12996..13222
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             13223..13403
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    13450..13575
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    14068..14301
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             14486..14530
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     promoter        14761..14971
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     promoter        14783..14979
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     rep_origin      14830..14965
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             15011..15613
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGAS"
     primer_bind     complement(15011..15030)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     15507..15527
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     misc_feature    15656..15773
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
ORIGIN
        1 ggctccggtg cccgtcagtg ggcagagcgc acatcgccca cagtccccga gaagttgggg
       61 ggaggggtcg gcaattgaac cggtgcctag agaaggtggc gcggggtaaa ctgggaaagt
      121 gatgtcgtgt actggctccg cctttttccc gagggtgggg gagaaccgta tataagtgca
      181 gtagtcgccg tgaacgttct ttttcgcaac gggtttgccg ccagaacaca ggtaagtgcc
      241 gtgtgtggtt cccgcgggcc tggcctcttt acgggttatg gcccttgcgt gccttgaatt
      301 acttccacct ggctgcagta cgtgattctt gatcccgagc ttcgggttgg aagtgggtgg
      361 gagagttcga ggccttgcgc ttaaggagcc ccttcgcctc gtgcttgagt tgaggcctgg
      421 cctgggcgct ggggccgccg cgtgcgaatc tggtggcacc ttcgcgcctg tctcgctgct
      481 ttcgataagt ctctagccat ttaaaatttt tgatgacctg ctgcgacgct ttttttctgg
      541 caagatagtc ttgtaaatgc gggccaagat ctgcacactg gtatttcggt ttttggggcc
      601 gcgggcggcg acggggcccg tgcgtcccag cgcacatgtt cggcgaggcg gggcctgcga
      661 gcgcggccac cgagaatcgg acgggggtag tctcaagctg gccggcctgc tctggtgcct
      721 ggcctcgcgc cgccgtgtat cgccccgccc tgggcggcaa ggctggcccg gtcggcacca
      781 gttgcgtgag cggaaagatg gccgcttccc ggccctgctg cagggagctc aaaatggagg
      841 acgcggcgct cgggagagcg ggcgggtgag tcacccacac aaaggaaaag ggcctttccg
      901 tcctcagccg tcgcttcatg tgactccacg gagtaccggg cgccgtccag gcacctcgat
      961 tagttctcga gcttttggag tacgtcgtct ttaggttggg gggaggggtt ttatgcgatg
     1021 gagtttcccc acactgagtg ggtggagact gaagttaggc cagcttggca cttgatgtaa
     1081 ttctccttgg aatttgccct ttttgagttt ggatcttggt tcattctcaa gcctcagaca
     1141 gtggttcaaa gtttttttct tccatttcag gtgtcgtgag gctagcaccg ccaccatgtc
     1201 gggatccaca cagcctgtgg cacagacgtg gagggccact gagccccgct acccgcccca
     1261 cagcctttcc tacccagtgc agatcgcccg gacgcacacg gacgtcgggc tcctggagta
     1321 ccagcaccac tcccgcgact atgcctccca cctgtcgccc ggctccatca tccagcccca
     1381 gcggcggagg ccctccctgc tgtctgagtt ccagcccggg aatgaacggt cccaggagct
     1441 ccacctgcgg ccagagtccc actcatacct gcccgagctg gggaagtcag agatggagtt
     1501 cattgaaagc aagcgccctc ggctagagct gctgcctgac cccctgctgc gaccgtcacc
     1561 cctgctggcc acgggccagc ctgcgggatc tgaagacctc accaaggacc gtagcctgac
     1621 gggcaagctg gaaccggtgt ctccccccag ccccccgcac actgaccctg agctggagct
     1681 ggtgccgcca cggctgtcca aggaggagct gatccagaac atggaccgcg tggaccgaga
     1741 gatcaccatg gtagagcagc agatctctaa gctgaagaag aagcagcaac agctggagga
     1801 ggaggctgcc aagccgcccg agcctgagaa gcccgtgtca ccgccgccca tcgagtcgaa
     1861 gcaccgcagc ctggtgcaga tcatctacga cgagaaccgg aagaaggctg aagctgcaca
     1921 tcggattctg gaaggcctgg ggccccaggt ggagctgccg ctgtacaacc agccctccga
     1981 cacccggcag tatcatgaga acatcaaaat aaaccaggcg atgcggaaga agctaatctt
     2041 gtacttcaag aggaggaatc acgctcggaa acaatgggag cagaagttct gccagcgcta
     2101 tgaccagctc atggaggcct gggagaagaa ggtggagcgc atcgagaaca acccccggcg
     2161 gcgggccaag gagagcaagg tgcgcgagta ctacgagaag cagttccctg agatccgcaa
     2221 gcagcgcgag ctgcaggagc gcatgcagag cagggtgggc cagcggggca gtgggctgtc
     2281 catgtcggcc gcccgcagcg agcacgaggt gtcagagatc atcgatggcc tctcagagca
     2341 ggagaacctg gagaagcaga tgcgccagct ggccgtgatc ccgcccatgc tgtacgacgc
     2401 tgaccagcag cgcatcaagt tcatcaacat gaacgggctt atggccgacc ccatgaaggt
     2461 gtacaaagac cgccaggtca tgaacatgtg gagtgagcag gagaaggaga ccttccggga
     2521 gaagttcatg cagcatccca agaactttgg cctgatcgca tcattcctgg agaggaagac
     2581 agtggctgag tgcgtcctct attactacct gactaagaag aatgagaact ataagagcct
     2641 ggtgagacgg agctatcggc gccgcggcaa gagccagcag cagcaacaac agcagcagca
     2701 gcagcagcag cagcagcagc agcagcccat gccccgcagc agccaggagg agaaagatga
     2761 gaaggagaag gaaaaggagg cggagaagga ggaggagaag ccggaggtgg agaacgacaa
     2821 ggaagacctc ctcaaggaga agacagacga cacctcaggg gaggacaacg acgagaagga
     2881 ggctgtggcc tccaaaggcc gcaaaactgc caacagccag ggaagacgca aaggccgcat
     2941 cacccgctca atggctaatg aggccaacag cgaggaggcc atcacccccc agcagagcgc
     3001 cgagctggcc tccatggagc tgaatgagag ttctcgctgg acagaagaag aaatggaaac
     3061 agccaagaaa ggtctcctgg aacacggccg caactggtcg gccatcgccc ggatggtggg
     3121 ctccaagact gtgtcgcagt gtaagaactt ctacttcaac tacaagaaga ggcagaacct
     3181 cgatgagatc ttgcagcagc acaagctgaa gatggagaag gagaggaacg cgcggaggaa
     3241 gaagaagaaa gcgccggcgg cggccagcga ggaggctgca ttcccgcccg tggtggagga
     3301 tgaggagatg gaggcgtcgg gcgtgagcgg aaatgaggag gagatggtgg aggaggctga
     3361 agccttacat gcctctggga atgaggtgcc cagaggggaa tgcagtggcc cagccactgt
     3421 caacaacagc tcagacaccg agagcatccc ctctcctcac actgaggccg ccaaggacac
     3481 agggcagaat gggcccaagc ccccagccac cctgggcgcc gacgggccac ccccagggcc
     3541 acccacccca ccaccggagg acatcccggc ccccactgag cccaccccgg cctctgaagc
     3601 caccggagcc cctacgcccc caccagcacc cccatcgccc tctgcacctc ctcctgtggt
     3661 ccccaaggag gagaaggagg aggagaccgc agcagcgccc ccagtggagg agggggagga
     3721 gcagaagccc cccgcggctg aggagctggc agtggacaca gggaaggccg aggagcccgt
     3781 caagagcgag tgcacggagg aagccgagga ggggccggcc aagggcaagg acgcggaggc
     3841 cgctgaggcc acggccgagg gggcgctcaa ggcagagaag aaggagggcg ggagcggcag
     3901 ggccaccaca gccaagagct cgggcgcccc ccaggacagc gactccagtg ctacctgcag
     3961 tgcagacgag gtggatgagg ccgagggcgg cgacaagaac cggctgctgt ccccaaggcc
     4021 cagcctcctc accccgactg gcgacccccg ggccaatgcc tcaccccaga agccactgga
     4081 cctgaagcag ctgaagcagc gagcggctgc catccccccc atccaggtca ccaaagtcca
     4141 tgagcccccc cgggaggacg cagctcccac caagccagct cccccagccc caccgccacc
     4201 gcaaaacctg cagccggaga gcgacgcccc tcagcagcct ggcagcagcc cccggggcaa
     4261 gagcaggagc ccggcacccc ccgccgacaa ggaggccttc gcagccgagg cccagaagct
     4321 gcctggggac cccccttgct ggacttccgg cctgcccttc cccgtgcccc cccgtgaggt
     4381 gatcaaggcc tccccgcatg ccccggaccc ctcagccttc tcctacgctc cacctggtca
     4441 cccactgccc ctgggcctcc atgacactgc ccggcccgtc ctgccgcgcc cacccaccat
     4501 ctccaacccg cctcccctca tctcctctgc caagcacccc agcgtcctcg agaggcaaat
     4561 aggtgccatc tcccaaggaa tgtcggtcca gctccacgtc ccgtactcag agcatgccaa
     4621 ggccccggtg ggccctgtca ccatggggct gcccctgccc atggacccca aaaagctggc
     4681 acccttcagc ggagtgaagc aggagcagct gtccccacgg ggccaggctg ggccaccgga
     4741 gagcctgggg gtgcccacag cccaggaggc gtccgtgctg agagggacag ctctgggctc
     4801 agttccgggc ggaagcatca ccaaaggcat tcccagcaca cgggtgccct cggacagcgc
     4861 catcacatac cgcggctcca tcacccacgg cacgccagct gacgtcctgt acaagggcac
     4921 catcaccagg atcatcggcg aggacagccc gagtcgcttg gaccgcggcc gggaggacag
     4981 cctgcccaag ggccacgtca tctacgaagg caagaagggc cacgtcttgt cctatgaggg
     5041 tggcatgtct gtgacccagt gctccaagga ggacggcaga agcagctcag gaccccccca
     5101 tgagacggcc gcccccaagc gcacctatga catgatggag ggccgcgtgg gcagagccat
     5161 ctcctcagcc agcatcgaag gtctcatggg ccgtgccatc ccgccggagc gacacagccc
     5221 ccaccacctc aaagagcagc accacatccg cgggtccatc acacaaggga tccctcggtc
     5281 ctacgtggag gcacaggagg actacctgcg tcgggaggcc aagctcctaa agcgggaggg
     5341 cacgcctccg cccccaccgc cctcacggga cctgaccgag gcctacaaga cgcaggccct
     5401 gggccccctg aagctgaagc cggcccatga gggcctggtg gccacggtga aggaggcggg
     5461 ccgctccatc catgagatcc cgcgcgagga gctgcggcac acgcccgagc tgcccctggc
     5521 cccgcggccg ctcaaggagg gctccatcac gcagggcacc ccgctcaagt acgacaccgg
     5581 cgcgtccacc actggctcca aaaagcacga cgtacgctcc ctcatcggca gccccggccg
     5641 gacgttccca cccgtgcacc cgctggatgt gatggccgac gcccgggcac tggaacgtgc
     5701 ctgctacgag gagagcctga agagccggcc agggaccgcc agcagctcgg ggggctccat
     5761 tgcgcgcggc gccccggtca ttgtgcctga gctgggtaag ccgcggcaga gccccctgac
     5821 ctatgaggac cacggggcac cctttgccgg ccacctccca cgaggttcgc ccgtgaccac
     5881 gcgggagccc acgccgcgcc tgcaggaggg cagcctttcg tccagcaagg catcccagga
     5941 ccgaaagctg acgtcgacgc ctcgtgagat cgccaagtcc ccgcacagca ccgtgcccga
     6001 gcaccaccca caccccatct cgccctatga gcacctgctt cggggcgtga gtggcgtgga
     6061 cctgtatcgc agccacatcc ccctggcctt cgaccccacc tccatacccc gcggcatccc
     6121 tctggacgca gccgctgcct actacctgcc ccgacacctg gcccccaacc ccacctaccc
     6181 gcacctgtac ccaccctacc tcatccgcgg ctaccccgac acggcggcgc tggagaaccg
     6241 gcagaccatc atcaatgact acatcacctc gcagcagatg caccacaacg cggccaccgc
     6301 catggcccag cgagctgata tgctgagggg cctctcgccc cgcgagtcct cgctggcact
     6361 caactacgct gcgggtcccc gaggcatcat cgacctgtcc caagtgccac acctgcctgt
     6421 gctcgtgccc ccgacaccag gcaccccagc caccgccatg gaccgccttg cctacctccc
     6481 caccgcgccc cagcccttca gcagccgcca cagcagctcc ccactctccc caggaggtcc
     6541 aacacacttg acaaaaccaa ccaccacgtc ctcgtccgag cgggagcgag accgggatcg
     6601 agagcgggac cgggatcggg agcgggaaaa gtccatcctc acgtccacca cgacggtgga
     6661 gcacgcaccc atctggagac ctggtacaga gcagagcagc ggcagcagcg gcgggggtgg
     6721 gggcagcagc agccgccccg cctcccactc ccatgcccac cagcactcgc ccatctcccc
     6781 tcggacccag gatgccctcc agcagagacc cagtgtgctt cacaacacag gcatgaaggg
     6841 tatcatcacc gctgtggagc ccagcacgcc cacggtcctg aggtccacct ccacctcctc
     6901 acccgttcgc ccggctgcca cattcccacc tgccacccac tgcccactgg gcggcaccct
     6961 cgatggggtc taccctaccc tcatggagcc cgtcttgctg cccaaggagg ccccccgggt
     7021 cgcccggcca gagcggcccc gagcagacac cggccatgcc ttcctcgcca agcccccagc
     7081 ccgctccggg ctggagcccg cctcctcccc cagcaagggc tcggagcccc ggcccctagt
     7141 gcctcctgtc tctggccacg ccaccatcgc ccgcacccct gcgaagaacc tcgcacctca
     7201 ccacgccagc ccggacccgc cggcgccacc tgcctcggcc tcggacccgc accgggaaaa
     7261 gactcaaagt aaaccctttt ccatccagga actggaactc cgttctctgg gttaccacgg
     7321 cagcagctac agccccgaag gggtggagcc cgtcagccct gtgagctcac ccagtctgac
     7381 ccacgacaag gggctcccca agcacctgga agagctcgac aagagccacc tggaggggga
     7441 gctgcggccc aagcagccag gccccgtgaa gcttggcggg gaggccgccc acctcccaca
     7501 cctgcggccg ctgcctgaga gccagccctc gtccagcccg ctgctccaga ccgccccagg
     7561 ggtcaaaggt caccagcggg tggtcaccct ggcccagcac atcagtgagg tcatcacaca
     7621 ggactacacc cggcaccacc cacagcagct cagcgcaccc ctgcccgccc ccctctactc
     7681 cttccctggg gccagctgcc ccgtcctgga cctccgccgc ccacccagtg acctctacct
     7741 cccgcccccg gaccatggtg ccccggcccg tggctccccc cacagcgaag ggggcaagag
     7801 gtctccagag ccaaacaaga cgtcggtctt gggtggtggt gaggacggta ttgaacctgt
     7861 gtccccaccg gagggcatga cggagccagg gcactcccgg agtgctgtgt acccgctgct
     7921 gtaccgggat ggggaacaga cggagcccag caggatgggc tccaagtctc caggcaacac
     7981 cagccagccg ccagccttct tcagcaagct gaccgagagc aactccgcca tggtcaagtc
     8041 caagaagcaa gagatcaaca agaagctgaa cacccacaac cggaatgagc ctgaatacaa
     8101 tatcagccag cctgggacgg agatcttcaa tatgcccgcc atcaccggaa caggccttat
     8161 gacctataga agccaggcgg tgcaggaaca tgccagcacc aacatggggc tggaggccat
     8221 aattagaaag gcactcatgg gtaaatatga ccagtgggaa gagtccccgc cgctcagcgc
     8281 caatgctttt aaccctctga atgccagtgc cagcctgccc gctgctatgc ccataaccgc
     8341 tgctgacgga cggagtgacc acacactcac ctcgccaggt ggcggcggga aggccaaggt
     8401 ctctggcaga cccagcagcc gaaaagccaa gtccccggcc ccgggcctgg catctgggga
     8461 ccggccaccc tctgtctcct cagtgcactc ggagggagac tgcaaccgcc ggacgccgct
     8521 caccaaccgc gtgtgggagg acaggccctc gtccgcaggt tccacgccat tcccctacaa
     8581 ccccctgatc atgcggctgc aggcgggtgt catggcttcc ccacccccac cgggcctccc
     8641 cgcgggcagc gggcccctcg ctggccccca ccacgcctgg gacgaggagc ccaagccact
     8701 gctctgctcg cagtacgaga cactctccga cagcgagtag taatgaacta gtccgtaact
     8761 tgaaagtatt tcgatttctt ggctttatat atcttgtgga aaggacgacc agctagccag
     8821 ataatacaga agacgcgtta agtcgacaat caacctctgg attacaaaat ttgtgaaaga
     8881 ttgactggta ttcttaacta tgttgctcct tttacgctat gtggatacgc tgctttaatg
     8941 cctttgtatc atgctattgc ttcccgtatg gctttcattt tctcctcctt gtataaatcc
     9001 tggttgctgt ctctttatga ggagttgtgg cccgttgtca ggcaacgtgg cgtggtgtgc
     9061 actgtgtttg ctgacgcaac ccccactggt tggggcattg ccaccacctg tcagctcctt
     9121 tccgggactt tcgctttccc cctccctatt gccacggcgg aactcatcgc cgcctgcctt
     9181 gcccgctgct ggacaggggc tcggctgttg ggcactgaca attccgtggt gttgtcgggg
     9241 aaatcatcgt cctttccttg gctgctcgcc tgtgttgcca cctggattct gcgcgggacg
     9301 tccttctgct acgtcccttc ggccctcaat ccagcggacc ttccttcccg cggcctgctg
     9361 ccggctctgc ggcctcttcc gcgtcttcgc cttcgccctc agacgagtcg gatctccctt
     9421 tgggccgcct ccccgcgtcg actttaagac caatgactta caaggcagct gtagatctta
     9481 gccacttttt aaaagaaaag gggggactgg aagggctaat tcactcccaa cgaagacaag
     9541 atctgctttt tgcttgtact gggtctctct ggttagacca gatctgagcc tgggagctct
     9601 ctggctaact agggaaccca ctgcttaagc ctcaataaag cttgccttga gtgcttcaag
     9661 tagtgtgtgc ccgtctgttg tgtgactctg gtaactagag atccctcaga cccttttagt
     9721 cagtgtggaa aatctctagc agtacgtata gtagttcatg tcatcttatt attcagtatt
     9781 tataacttgc aaagaaatga atatcagaga gtgagaggaa cttgtttatt gcagcttata
     9841 atggttacaa ataaagcaat agcatcacaa atttcacaaa taaagcattt ttttcactgc
     9901 attctagttg tggtttgtcc aaactcatca atgtatctta tcatgtctgg ctctagctat
     9961 cccgccccta actccgccca tcccgcccct aactccgccc agttccgccc attctccgcc
    10021 ccatggctga ctaatttttt ttatttatgc agaggccgag gccgcctcgg cctctgagct
    10081 attccagaag tagtgaggag gcttttttgg aggcctaggg acgtacccaa ttcgccctat
    10141 agtgagtcgt attacgcgcg ctcactggcc gtcgttttac aacgtcgtga ctgggaaaac
    10201 cctggcgtta cccaacttaa tcgccttgca gcacatcccc ctttcgccag ctggcgtaat
    10261 agcgaagagg cccgcaccga tcgcccttcc caacagttgc gcagcctgaa tggcgaatgg
    10321 gacgcgccct gtagcggcgc attaagcgcg gcgggtgtgg tggttacgcg cagcgtgacc
    10381 gctacacttg ccagcgccct agcgcccgct cctttcgctt tcttcccttc ctttctcgcc
    10441 acgttcgccg gctttccccg tcaagctcta aatcgggggc tccctttagg gttccgattt
    10501 agtgctttac ggcacctcga ccccaaaaaa cttgattagg gtgatggttc acgtagtggg
    10561 ccatcgccct gatagacggt ttttcgccct ttgacgttgg agtccacgtt ctttaatagt
    10621 ggactcttgt tccaaactgg aacaacactc aaccctatct cggtctattc ttttgattta
    10681 taagggattt tgccgatttc ggcctattgg ttaaaaaatg agctgattta acaaaaattt
    10741 aacgcgaatt ttaacaaaat attaacgctt acaatttagg tggcactttt cggggaaatg
    10801 tgcgcggaac ccctatttgt ttatttttct aaatacattc aaatatgtat ccgctcatga
    10861 gacaataacc ctgataaatg cttcaataat attgaaaaag gaagagtatg agtattcaac
    10921 atttccgtgt cgcccttatt cccttttttg cggcattttg ccttcctgtt tttgctcacc
    10981 cagaaacgct ggtgaaagta aaagatgctg aagatcagtt gggtgcacga gtgggttaca
    11041 tcgaactgga tctcaacagc ggtaagatcc ttgagagttt tcgccccgaa gaacgttttc
    11101 caatgatgag cacttttaaa gttctgctat gtggcgcggt attatcccgt attgacgccg
    11161 ggcaagagca actcggtcgc cgcatacact attctcagaa tgacttggtt gagtactcac
    11221 cagtcacaga aaagcatctt acggatggca tgacagtaag agaattatgc agtgctgcca
    11281 taaccatgag tgataacact gcggccaact tacttctgac aacgatcgga ggaccgaagg
    11341 agctaaccgc ttttttgcac aacatggggg atcatgtaac tcgccttgat cgttgggaac
    11401 cggagctgaa tgaagccata ccaaacgacg agcgtgacac cacgatgcct gtagcaatgg
    11461 caacaacgtt gcgcaaacta ttaactggcg aactacttac tctagcttcc cggcaacaat
    11521 taatagactg gatggaggcg gataaagttg caggaccact tctgcgctcg gcccttccgg
    11581 ctggctggtt tattgctgat aaatctggag ccggtgagcg tgggtctcgc ggtatcattg
    11641 cagcactggg gccagatggt aagccctccc gtatcgtagt tatctacacg acggggagtc
    11701 aggcaactat ggatgaacga aatagacaga tcgctgagat aggtgcctca ctgattaagc
    11761 attggtaact gtcagaccaa gtttactcat atatacttta gattgattta aaacttcatt
    11821 tttaatttaa aaggatctag gtgaagatcc tttttgataa tctcatgacc aaaatccctt
    11881 aacgtgagtt ttcgttccac tgagcgtcag accccgtaga aaagatcaaa ggatcttctt
    11941 gagatccttt ttttctgcgc gtaatctgct gcttgcaaac aaaaaaacca ccgctaccag
    12001 cggtggtttg tttgccggat caagagctac caactctttt tccgaaggta actggcttca
    12061 gcagagcgca gataccaaat actgttcttc tagtgtagcc gtagttaggc caccacttca
    12121 agaactctgt agcaccgcct acatacctcg ctctgctaat cctgttacca gtggctgctg
    12181 ccagtggcga taagtcgtgt cttaccgggt tggactcaag acgatagtta ccggataagg
    12241 cgcagcggtc gggctgaacg gggggttcgt gcacacagcc cagcttggag cgaacgacct
    12301 acaccgaact gagataccta cagcgtgagc tatgagaaag cgccacgctt cccgaaggga
    12361 gaaaggcgga caggtatccg gtaagcggca gggtcggaac aggagagcgc acgagggagc
    12421 ttccaggggg aaacgcctgg tatctttata gtcctgtcgg gtttcgccac ctctgacttg
    12481 agcgtcgatt tttgtgatgc tcgtcagggg ggcggagcct atggaaaaac gccagcaacg
    12541 cggccttttt acggttcctg gccttttgct ggccttttgc tcacatgttc tttcctgcgt
    12601 tatcccctga ttctgtggat aaccgtatta ccgcctttga gtgagctgat accgctcgcc
    12661 gcagccgaac gaccgagcgc agcgagtcag tgagcgagga agcggaagag cgcccaatac
    12721 gcaaaccgcc tctccccgcg cgttggccga ttcattaatg cagctggcac gacaggtttc
    12781 ccgactggaa agcgggcagt gagcgcaacg caattaatgt gagttagctc actcattagg
    12841 caccccaggc tttacacttt atgcttccgg ctcgtatgtt gtgtggaatt gtgagcggat
    12901 aacaatttca cacaggaaac agctatgacc atgattacgc caagcgcgca attaaccctc
    12961 actaaaggga acaaaagctg gagctgcaag cttaatgtag tcttatgcaa tactcttgta
    13021 gtcttgcaac atggtaacga tgagttagca acatgcctta caaggagaga aaaagcaccg
    13081 tgcatgccga ttggtggaag taaggtggta cgatcgtgcc ttattaggaa ggcaacagac
    13141 gggtctgaca tggattggac gaaccactga attgccgcat tgcagagata ttgtatttaa
    13201 gtgcctagct cgatacataa acgggtctct ctggttagac cagatctgag cctgggagct
    13261 ctctggctaa ctagggaacc cactgcttaa gcctcaataa agcttgcctt gagtgcttca
    13321 agtagtgtgt gcccgtctgt tgtgtgactc tggtaactag agatccctca gaccctttta
    13381 gtcagtgtgg aaaatctcta gcagtggcgc ccgaacaggg acttgaaagc gaaagggaaa
    13441 ccagaggagc tctctcgacg caggactcgg cttgctgaag cgcgcacggc aagaggcgag
    13501 gggcggcgac tggtgagtac gccaaaaatt ttgactagcg gaggctagaa ggagagagat
    13561 gggtgcgaga gcgtcagtat taagcggggg agaattagat cgcgatggga aaaaattcgg
    13621 ttaaggccag ggggaaagaa aaaatataaa ttaaaacata tagtatgggc aagcagggag
    13681 ctagaacgat tcgcagttaa tcctggcctg ttagaaacat cagaaggctg tagacaaata
    13741 ctgggacagc tacaaccatc ccttcagaca ggatcagaag aacttagatc attatataat
    13801 acagtagcaa ccctctattg tgtgcatcaa aggatagaga taaaagacac caaggaagct
    13861 ttagacaaga tagaggaaga gcaaaacaaa agtaagacca ccgcacagca agcggccgct
    13921 gatcttcaga cctggaggag gagatatgag ggacaattgg agaagtgaat tatataaata
    13981 taaagtagta aaaattgaac cattaggagt agcacccacc aaggcaaaga gaagagtggt
    14041 gcagagagaa aaaagagcag tgggaatagg agctttgttc cttgggttct tgggagcagc
    14101 aggaagcact atgggcgcag cgtcaatgac gctgacggta caggccagac aattattgtc
    14161 tggtatagtg cagcagcaga acaatttgct gagggctatt gaggcgcaac agcatctgtt
    14221 gcaactcaca gtctggggca tcaagcagct ccaggcaaga atcctggctg tggaaagata
    14281 cctaaaggat caacagctcc tggggatttg gggttgctct ggaaaactca tttgcaccac
    14341 tgctgtgcct tggaatgcta gttggagtaa taaatctctg gaacagattt ggaatcacac
    14401 gacctggatg gagtgggaca gagaaattaa caattacaca agcttaatac actccttaat
    14461 tgaagaatcg caaaaccagc aagaaaagaa tgaacaagaa ttattggaat tagataaatg
    14521 ggcaagtttg tggaattggt ttaacataac aaattggctg tggtatataa aattattcat
    14581 aatgatagta ggaggcttgg taggtttaag aatagttttt gctgtacttt ctatagtgaa
    14641 tagagttagg cagggatatt caccattatc gtttcagacc cacctcccaa ccccgagggg
    14701 acccatgcat tgcatctcaa ttagtcagca accaggtgtg gaaagtcccc aggctcccca
    14761 gcaggcagaa gtatgcaaag catgcgtctc aattagtcag caaccatagt cccgccccta
    14821 actccgccca tcccgcccct aactccgccc agttccgccc attctccgcc ccatggctga
    14881 ctaatttttt ttatttatgc agaggccgag gccgcctcgg cctctgagct attccagaag
    14941 tagtgaggag gcttttttgg aggcctaggc ttttgcaaaa agctttctag aggtaccacc
    15001 ggagcttacc atgaccgagt acaagcccac ggtgcgcctc gccacccgcg acgacgtccc
    15061 cagggccgta cgcaccctcg ccgccgcgtt cgccgactac cccgccacgc gccacaccgt
    15121 cgatccggac cgccacatcg agcgggtcac cgagctgcaa gaactcttcc tcacgcgcgt
    15181 cgggctcgac atcggcaagg tgtgggtcgc ggacgacggc gccgccgtgg cggtctggac
    15241 cacgccggag agcgtcgaag cgggggcggt gttcgccgag atcggcccgc gcatggccga
    15301 gttgagcggt tcccggctgg ccgcgcagca acagatggaa ggcctcctgg cgccgcaccg
    15361 gcccaaggag cccgcgtggt tcctggccac cgtcggcgtc tcgcccgacc accagggcaa
    15421 gggtctgggc agcgccgtcg tgctccccgg agtggaggcg gccgagcgcg ccggggtgcc
    15481 cgccttcctg gagacctccg cgccccgcaa cctccccttc tacgagcggc tcggcttcac
    15541 cgtcaccgcc gacgtcgagg tgcccgaagg accgcgcacc tggtgcatga cccgcaagcc
    15601 cggtgccagt taacgaattc tagatcttga gacaaatggc agtattcatc cacaatttta
    15661 aaagaaaagg ggggattggg gggtacagtg caggggaaag aatagtagac ataatagcaa
    15721 cagacataca aactaaagaa ttacaaaaac aaattacaaa aattcaaaat tttcgggttt
    15781 attacaggga cagcagagat ccactttggc gccggctcga ggcctgcagg tgcaaagatg
    15841 gataaagttt taaacagaga ggaatctttg cagctaatgg accttctagg tcttgaaagg
    15901 agtgggaatt
//
