LOCUS       sequence-353415-        1856 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Bacterial Expression plasmid for SARS-CoV-2 ORF10 protein.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 1856)
  TITLE     SARS-CoV-2 protein E. coli expression constructs, tagged, for 
            protein expression and/or purification
REFERENCE   2  (bases 1 to 1856)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pGBW-m4046897
FEATURES             Location/Qualifiers
     source          1..1856
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     RBS             1..23
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             145..165
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQS"
     CDS             166..192
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     rep_origin      313..901
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     802..821
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             1007..1666
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     complement(1073..1092)
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     terminator      1737..1771
                     /label=lambda t0 terminator
                     /note="minimal transcription terminator from phage lambda 
                     (Scholtissek and Grosse, 1987)"
     primer_bind     1799..1818
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        1799..1817
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    1818..1842
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
ORIGIN
        1 tttgtttaac tttaagaagg agactcttct atgggctata taaacgtttt cgcttttccg
       61 tttacgatat atagtctact cttgtgcaga atgaattctc gtaactacat agcacaagta
      121 gatgtagtta actttaatct cacagagaac ctgtactttc aaagctaccc gtatgacgtt
      181 cctgattatg cctaaccggc ttatcggtca gtttcacctg atttacgtaa aaacccgctt
      241 cggcgggttt ttgcttttgg aggggcagaa agatgaatga ctgtccacga cgctataccc
      301 aaaagaaaaa gcttgagatc ctttttttct gcgcgtaatc tgctgcttgc aaacaaaaaa
      361 accaccgcta ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc tttttccgaa
      421 ggtaactggc ttcagcagag cgcagatacc aaatactgtt cttctagtgt agccgtagtt
      481 aggccaccac ttcaagaact ctgtagcacc gcctacatac ctcgctctgc taatcctgtt
      541 accagtggct gctgccagtg gcgataagtc gtgtcttacc gggttggact caagacgata
      601 gttaccggat aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac agcccagctt
      661 ggagcgaacg acctacaccg aactgagata cctacagcgt gagctatgag aaagcgccac
      721 gcttcccgaa gggagaaagg cggacaggta tccggtaagc ggcagggtcg gaacaggaga
      781 gcgcacgagg gagcttccag ggggaaacgc ctggtatctt tatagtcctg tcgggtttcg
      841 ccacctctga cttgagcgtc gatttttgtg atgctcgtca ggggggcgga gcctatggaa
      901 attgatcggg cacgtaagag gttccaactt tcaccataat gaaataagat cactaccggg
      961 cgtatttttt gagttatcga gattttcagg agctaaggaa gctaaaatgg agaaaaaaat
     1021 cactggatat accaccgttg atatatccca atggcatcgt aaagaacatt ttgaggcatt
     1081 tcagtcagtt gctcaatgta cctataacca gaccgttcag ctggatatta cggccttttt
     1141 aaagaccgta aagaaaaata agcacaagtt ttatccggcc tttattcaca ttcttgcccg
     1201 cctgatgaat gctcatccgg aatttcgtat ggcaatgaaa gacggtgagc tggtgatatg
     1261 ggatagtgtt cacccttgtt acaccgtttt ccatgagcaa actgaaacgt tttcatcgct
     1321 ctggagtgaa taccacgacg atttccggca gtttctacac atatattcgc aagatgtggc
     1381 gtgttacggt gaaaacctgg cctatttccc taaagggttt attgagaata tgtttttcgt
     1441 ttcagccaat ccctgggtga gtttcaccag ttttgattta aacgtggcca atatggacaa
     1501 cttcttcgcc cccgttttca ccatgggcaa atattatacg caaggcgaca aggtgctgat
     1561 gccgctggcg attcaggttc atcatgccgt ttgtgatggc ttccatgtcg gcagaatgct
     1621 taatgaatta caacagtact gcgatgagtg gcagggcggg gcgtaatttg atatcgagct
     1681 cgcttggact cctgttgata gatccagtaa tgacctcaga actccatctg gatttgttca
     1741 gaacgctcgg ttgccgccgg gcgtttttta ttggtcatcg atctcgatcc cgcgaaatta
     1801 atacgactca ctatagggga attgtgagcg gataacaatt cccctctaga aataat
//
