LOCUS       sequence-35342-e         874 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 874)
  AUTHORS   Hussein R, Lim HN
  TITLE     Disruption of small RNA signaling caused by competition for Hfq.
  JOURNAL   Proc Natl Acad Sci U S A. 2011 Jan 18;108(3):1110-5. doi: 
            10.1073/pnas.1010082108. Epub 2010 Dec 28.
  PUBMED    21189298
REFERENCE   2  (bases 1 to 874)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1073/pnas.1010082108"; journalName: "Proc Natl Acad Sci U S A"; 
            date: "2011-01-18- 18"; volume: "108"; issue: "3"; pages: "1110-5"
COMMENT     Sequence Label: HL 4218 (=HL 716 + pHL 1411)
FEATURES             Location/Qualifiers
     source          1..874
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    206..222
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        280..384
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(603..622)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
ORIGIN
        1 cctttacgca tgctatcatg aactttcagc gtattctgac tcataaggtg gctcctaccc
       61 gtgatccctt gacggaacat tcaagcaaaa gcctggttcc gccgatttat cgctgcggca
      121 aataacgcag cgggtttacg gatttcccct tgtaacgaat ttcaaaatgc aagcgtgttg
      181 aactggttcc ggtgtcgaca gtatcttgtt atccgctcac aatgtcaatg ttatccgctc
      241 acatttatga cgtcaggtgg cacttttcgg ggaaatgtgc gcggaacccc tatttgttta
      301 tttttctaaa tacattcaaa tatgtatccg ctcatgagac aataaccctg ataaatgctt
      361 caataatatt gaaaaaggaa gagtatgagt attcaacatt tccgtgtcgc ccttattccc
      421 ttttttgcgg cattttgcct tcctgttttt gctcacccag aaacgctggt gaaagtaaaa
      481 gatgctgaag atcagttggg tgcacgagtg ggttacatcg aactggatct caacagcggt
      541 aagatccttg agagttttcg ccccgaagaa cgttttccaa tgatgagcac ttttaaagtt
      601 ctgctatgtg gcgcggtatt atcccgtatt gacgccgggc aagagcaact cggtcgccgc
      661 atacactatt ctcagaatga cttggttgag tactcaccag tcacagaaaa gcatcttacg
      721 gatggcatga cagtaagaga attatgcagt gctgccataa ccatgagtga taacactgcg
      781 gccaacttac ttctgacaac gatcggagga ccgaaggagc taaccgcttt tttgcacaac
      841 atgggggatc atgtaactcg ccttgatcgt tggg
//
