LOCUS       sequence-353420-        5579 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Insect cell expression of GCP3.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5579)
  AUTHORS   Wieczorek M, Ti SC, Urnavicius L, Molloy KR, Aher A, Chait BT, 
            Kapoor TM
  TITLE     Biochemical reconstitutions reveal principles of human gamma-TuRC 
            assembly and function.
  JOURNAL   J Cell Biol. 2021 Mar 1;220(3). pii: 211719. doi: 
            10.1083/jcb.202009146.
  PUBMED    33496729
REFERENCE   2  (bases 1 to 5579)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Cell 
            Biol."; date: "2021-03-1"; volume: "220(3). pii: 211719. doi"; 
            pages: " 10.1083/jcb.202009146"
COMMENT     Sequence Label: pACEBac1-GCP3
FEATURES             Location/Qualifiers
     source          1..5579
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      190..778
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     679..698
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     mobile_element  913..1136
                     /mobile_element_type="transposon:Tn7"
                     /label=Tn7R
                     /note="mini-Tn7 element (right end of the Tn7 transposon)"
     CDS             complement(1203..1736)
                     /codon_start=1
                     /gene="aacC1"
                     /product="gentamycin acetyltransferase"
                     /label=GmR
                     /note="confers resistance to gentamycin"
                     /translation="MLRSSNDVTQQGSRPKTKLGGSSMGIIRTCRLGPDQVKSMRAALD
                     LFGREFGDVATYSQHQPDSDYLGNLLRSKTFIALAAFDQEAVVGALAAYVLPKFEQPRS
                     EIYIYDLAVSGEHRRQGIATALINLLKHEANALGAYVIYVQADYGDDPAVALYTKLGIR
                     EEVMHFDIDPSTAT"
     promoter        complement(join(1925..1930,1931..1947,1948..1953))
                     /gene="intI1 (promoter lies within the coding sequence)"
                     /label=Pc promoter
                     /note="class 1 integron promoter"
     protein_bind    complement(2024..2057)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     promoter        2134..2225
                     /gene="polh from Autographa californica"
                     /label=polyhedrin promoter
                     /note="promoter for the baculovirus polyhedrin gene"
     primer_bind     2150..2167
                     /label=Polyhedrin-F
                     /note="Polyhedrin promoter, forward primer"
     polyA_signal    5159..5293
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(5196..5215)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     5250..5269
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     mobile_element  5414..5579
                     /mobile_element_type="transposon:Tn7"
                     /label=Tn7L
                     /note="mini-Tn7 element (left end of the Tn7 transposon)"
ORIGIN
        1 accggttgac ttgggtcaac tgtcagacca agtttactca tatatacttt agattgattt
       61 aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac
      121 caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa
      181 aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc
      241 accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt
      301 aactggcttc agcagagcgc agataccaaa tactgttctt ctagtgtagc cgtagttagg
      361 ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc
      421 agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt
      481 accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga
      541 gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct
      601 tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg
      661 cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca
      721 cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa
      781 cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt
      841 ctttcctgcg ttatcccctg attgacttgg gtcgctcttc ctgtggatgc gcagatgccc
      901 tgcgtaagcg ggtgtgggcg gacaataaag tcttaaactg aacaaaatag atctaaacta
      961 tgacaataaa gtcttaaact agacagaata gttgtaaact gaaatcagtc cagttatgct
     1021 gtgaaaaagc atactggact tttgttatgg ctaaagcaaa ctcttcattt tctgaagtgc
     1081 aaattgcccg tcgtattaaa gaggggcgtg gccaagggca tgtaaagact atattcgcgg
     1141 cgttgtgaca atttaccgaa caactccgcg gccgggaagc cgatctcggc ttgaacgaat
     1201 tgttaggtgg cggtacttgg gtcgatatca aagtgcatca cttcttcccg tatgcccaac
     1261 tttgtataga gagccactgc gggatcgtca ccgtaatctg cttgcacgta gatcacataa
     1321 gcaccaagcg cgttggcctc atgcttgagg agattgatga gcgcggtggc aatgccctgc
     1381 ctccggtgct cgccggagac tgcgagatca tagatataga tctcactacg cggctgctca
     1441 aacttgggca gaacgtaagc cgcgagagcg ccaacaaccg cttcttggtc gaaggcagca
     1501 agcgcgatga atgtcttact acggagcaag ttcccgaggt aatcggagtc cggctgatgt
     1561 tgggagtagg tggctacgtc tccgaactca cgaccgaaaa gatcaagagc agcccgcatg
     1621 gatttgactt ggtcagggcc gagcctacat gtgcgaatga tgcccatact tgagccacct
     1681 aactttgttt tagggcgact gccctgctgc gtaacatcgt tgctgctgcg taacatcgtt
     1741 gctgctccat aacatcaaac atcgacccac ggcgtaacgc gcttgctgct tggatgcccg
     1801 aggcatagac tgtacaaaaa aacagtcata acaagccatg aaaaccgcca ctgcgccgtt
     1861 accaccgctg cgttcggtca aggttctgga ccagttgcgt gagcgcatac gctacttgca
     1921 ttacagttta cgaaccgaac aggcttatgt caactgggtt cgtgccttca tccgtttcca
     1981 cggtgtgcgt cacccggcaa ccttgggcag cagcgaagtc gccataactt cgtatagcat
     2041 acattatacg aagttatctg taactataac ggtcctaagg tagcgagttt aaacactagt
     2101 atcgattcgc gacctactcc ggaatattaa tagatcatgg agataattaa aatgataacc
     2161 atctcgcaaa taaataagta ttttactgtt ttcgtaacag ttttgtaata aaaaaaccta
     2221 taaatattcc ggattattca taccgtccca ccatcgggcg cggatcccgg tccgaagcgc
     2281 gcggaattca aaggcctacg tcgacatggc gaccccggac cagaagtcgc cgaacgttct
     2341 gctgcagaac ctgtgctgca ggatcctggg caggagcgaa gctgatgtag cccagcagtt
     2401 ccagtatgct gtgcgggtga ttggcagcaa cttcgcccca actgttgaaa gagatgaatt
     2461 tttagtagct gaaaaaatca agaaagagct tattcgacaa cgaagagaag cagatgctgc
     2521 attattttca gaactccaca gaaaacttca ttcacaggga gttttgaaaa ataaatggtc
     2581 aatactctac ctcttgctga gcctcagtga ggacccacgc aggcagccaa gcaaggtttc
     2641 tagctatgct acgttatttg ctcaggcctt accaagagat gcccactcaa ccccttacta
     2701 ctatgccagg cctcagaccc ttcccctgag ctaccaagat cggagtgccc agtcagccca
     2761 gagctccggc agcgtgggca gcagtggcat cagcagcatt ggcctgtgtg ccctcagtgg
     2821 ccccgcgcct gcgccacaat ctctcctccc aggacagtct aatcaagctc caggagtagg
     2881 agattgcctt cgacagcagt tggggtcacg actcgcatgg actttaactg caaatcagcc
     2941 ttcttcacaa gccactacct caaaaggtgt ccccagtgct gtgtctcgca acatgacaag
     3001 gtccaggaga gaaggggata cgggtggtac tatggaaatt acagaagcag ctctggtaag
     3061 ggacattttg tacgtctttc agggcataga tggcaaaaac atcaaaatga acaacactga
     3121 aaattgttac aaagtagaag gaaaggcaaa tctaagtagg tctttgagag acacagcagt
     3181 caggctttct gagttgggat ggttgcataa taaaatcaga agatacacgg accagaggag
     3241 cctggaccgc tcattcggac tcgtcgggca gagcttttgt gctgccttgc accaggaact
     3301 cagagaatac tatcgattgc tctctgtttt acattctcag ctacaactag aggatgacca
     3361 gggtgtgaat ttgggacttg agagtagttt aacacttcgg cgcctcctgg tttggaccta
     3421 tgatcccaaa atacgactga agacccttgc ggccctagtg gaccactgcc aaggaaggaa
     3481 aggaggtgag ctggcctcag ctgtccacgc ctacacaaaa acaggagacc cgtacatgcg
     3541 gtctctggtg cagcacatcc tcagcctcgt gtctcatcct gttttgagct tcctgtaccg
     3601 ctggatatat gatggggagc ttgaggacac ttaccacgaa ttttttgtag catcagatcc
     3661 aacagttaaa acagatcgac tgtggcacga caagtatact ttgaggaaat cgatgattcc
     3721 ttcgtttatg acgatggatc agtctaggaa ggtccttttg ataggaaaat caataaattt
     3781 cttgcaccaa gtttgtcatg atcagactcc cactacaaag atgatagctg tgaccaagtc
     3841 tgcagagtca ccccaggacg ctgcagacct attcacagac ttggaaaatg catttcaggg
     3901 gaagattgat gctgcttatt ttgagaccag caaatacctg ttggatgttc tcaataaaaa
     3961 gtacagcttg ctggaccaca tgcaggcaat gaggcggtac ctgcttcttg gtcaaggaga
     4021 ctttataagg cacttaatgg acttgctaaa accagaactt gtccgtccag ctacgacttt
     4081 gtatcagcat aacttgactg gaattctaga aaccgctgtc agagccacca acgcacagtt
     4141 tgacagtcct gagatcctgc gaaggctgga cgtgcggctg ctggaggtct ctccaggtga
     4201 cactggatgg gatgtcttca gcctcgatta tcatgttgac ggaccaattg caactgtgtt
     4261 tactcgagaa tgtatgagcc actacctaag agtatttaac ttcctctgga gggcgaagcg
     4321 gatggaatac atcctcactg acatacggaa gggacacatg tgcaatgcaa agctcctgag
     4381 aaacatgcca gagttctccg gggtgctgca ccagtgtcac attttggcct ctgagatggt
     4441 ccatttcatt catcagatgc agtattacat cacatttgag gtgcttgaat gttcttggga
     4501 tgagctttgg aacaaagtcc agcaggccca ggatttggat cacatcattg ctgcacacga
     4561 ggtgttctta gacaccatca tctcccgctg cctgctggac agtgactcca gggcactttt
     4621 aaatcaactt agagctgtgt ttgatcaaat tattgaactt cagaatgctc aagatgcaat
     4681 atacagagct gctctggaag aattgcagag acgattacag tttgaagaga aaaagaaaca
     4741 gcgtgaaatt gagggccagt ggggagtgac ggcagcagag gaagaggagg aaaataagag
     4801 gattggagaa tttaaagaat ctataccaaa aatgtgctca cagttgcgaa tattgaccca
     4861 tttctaccag ggtatcgtgc agcagttttt ggtgttactg acgaccagct ctgacgagag
     4921 tcttcggttt cttagcttca ggctggactt caacgagcat tacaaagcca gggagcccag
     4981 gctccgtgtg tctctgggta ccagggggcg gcgcagctcc cacacgtgaa agcttgtcga
     5041 gaagtactag aggatcataa tcagccatac cacatttgta gaggttttac ttgctttaaa
     5101 aaacctccca cacctccccc tgaacctgaa acataaaatg aatgcaattg ttgttgttaa
     5161 cttgtttatt gcagcttata atggttacaa ataaagcaat agcatcacaa atttcacaaa
     5221 taaagcattt ttttcactgc attctagttg tggtttgtcc aaactcatca atgtatctta
     5281 tcatgtctgg atctgatcac tgcttgagcc tagaagatcc ggctgctaac aaagcccgaa
     5341 aggaagctga gttggctgct gccaccgctg agcaataact atcataaccc ctagggtata
     5401 cccatctaat tggaaccaga taagtgaaat ctagttccaa actattttgt catttttaat
     5461 tttcgtatta gcttacgacg ctacacccag ttcccatcta ttttgtcact cttccctaaa
     5521 taatccttaa aaactccatt tccacccctc ccagttccca actattttgt ccgcccaca
//
