LOCUS       sequence-353421-       17128 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 17128)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 17128)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF1872
FEATURES             Location/Qualifiers
     source          1..17128
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        1..1179
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          232..1170
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     1127..1147
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     regulatory      9740..9749
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     misc_feature    10066..10654
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(10119..10139)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(10537..10548)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             10726..10959
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    11037..11158
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(11074..11093)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     11128..11147
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      11198..11333
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(11353..11372)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(11354..11372)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(11382..11399)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(11382..11398)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(11391..11413)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      11540..11995
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(11627..11646)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     11837..11858
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        12021..12125
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(12126..12194,12195..12986)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(12344..12363)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      13157..13745
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     13646..13665
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     13899..13916
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    14033..14054
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(14069..14074,14075..14092,14093..14099)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    14107..14123
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     14112..14134
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     14131..14147
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     14131..14147
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     14166..14186
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        14168..14186
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        14214..14440
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             14441..14621
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    14668..14793
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    15286..15519
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             15704..15748
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     promoter        15979..16189
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     promoter        16001..16197
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     rep_origin      16048..16183
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             16229..16831
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGAS"
     primer_bind     complement(16229..16248)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     16725..16745
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     misc_feature    16874..16991
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
ORIGIN
        1 ggctccggtg cccgtcagtg ggcagagcgc acatcgccca cagtccccga gaagttgggg
       61 ggaggggtcg gcaattgaac cggtgcctag agaaggtggc gcggggtaaa ctgggaaagt
      121 gatgtcgtgt actggctccg cctttttccc gagggtgggg gagaaccgta tataagtgca
      181 gtagtcgccg tgaacgttct ttttcgcaac gggtttgccg ccagaacaca ggtaagtgcc
      241 gtgtgtggtt cccgcgggcc tggcctcttt acgggttatg gcccttgcgt gccttgaatt
      301 acttccacct ggctgcagta cgtgattctt gatcccgagc ttcgggttgg aagtgggtgg
      361 gagagttcga ggccttgcgc ttaaggagcc ccttcgcctc gtgcttgagt tgaggcctgg
      421 cctgggcgct ggggccgccg cgtgcgaatc tggtggcacc ttcgcgcctg tctcgctgct
      481 ttcgataagt ctctagccat ttaaaatttt tgatgacctg ctgcgacgct ttttttctgg
      541 caagatagtc ttgtaaatgc gggccaagat ctgcacactg gtatttcggt ttttggggcc
      601 gcgggcggcg acggggcccg tgcgtcccag cgcacatgtt cggcgaggcg gggcctgcga
      661 gcgcggccac cgagaatcgg acgggggtag tctcaagctg gccggcctgc tctggtgcct
      721 ggcctcgcgc cgccgtgtat cgccccgccc tgggcggcaa ggctggcccg gtcggcacca
      781 gttgcgtgag cggaaagatg gccgcttccc ggccctgctg cagggagctc aaaatggagg
      841 acgcggcgct cgggagagcg ggcgggtgag tcacccacac aaaggaaaag ggcctttccg
      901 tcctcagccg tcgcttcatg tgactccacg gagtaccggg cgccgtccag gcacctcgat
      961 tagttctcga gcttttggag tacgtcgtct ttaggttggg gggaggggtt ttatgcgatg
     1021 gagtttcccc acactgagtg ggtggagact gaagttaggc cagcttggca cttgatgtaa
     1081 ttctccttgg aatttgccct ttttgagttt ggatcttggt tcattctcaa gcctcagaca
     1141 gtggttcaaa gtttttttct tccatttcag gtgtcgtgag gctagcaccg ccaccatgag
     1201 gggccggcgg ggcaggccgc ccaagcagcc cgcggctccc gctgcggagc gctgcgcccc
     1261 ggccccgccg ccaccgccgc cgccgcccac gtccggaccc atcggggggc tccgctcgcg
     1321 gcaccgcggc agcagccggg gcaggtgggc cgccgcccag gctgaggtgg cgcccaagac
     1381 gcggctgagc tcgcccaggg ggggcagcag tagccggagg aagccgccgc cgccgccgcc
     1441 ggcccccccc agcaccagcg ccccgggccg gggggggcga ggaggcgggg gcggcaggac
     1501 ggggggcggg ggcggcggcg gccacctggc ccggaccacc gcggcccgga gggccgtcaa
     1561 caaagtggtg tacgatgacc acgagagcga ggaggaggag gaagaggagg acatggtctc
     1621 cgaggaggag gaggaggagg acggcgacgc cgaggagacc caggattctg aggacgacga
     1681 ggaggatgag atggaagagg acgacgatga ctccgattat ccggaggaga tggaagacga
     1741 cgacgacgac gccagttact gcacggaaag cagcttcagg agccatagta cctacagcag
     1801 cactccaggt aggcgaaaac caagagtaca tcggcctcgt tctcctatat tggaagaaaa
     1861 agacatcccg ccccttgaat ttcccaagtc ctctgaggat ttaatggtgc ctaatgagca
     1921 tataatgaat gtcattgcca tttacgaggt actgcggaac tttggcactg ttttgagatt
     1981 atctcctttt cgctttgagg acttttgtgc agctctggtg agccaagagc agtgcacact
     2041 catggcagag atgcatgttg tgcttttgaa agcagttctg cgtgaagaag acacttccaa
     2101 tactaccttt ggacctgctg atctgaaaga tagcgttaat tccacactgt atttcataga
     2161 tgggatgacg tggccagagg tgctgcgggt gtactgtgag agtgataagg agtaccatca
     2221 cgttcttcct taccaagagg cagaggacta cccatatgga ccagtagaga acaagatcaa
     2281 agttctacag tttctagtcg atcagtttct tacaacaaat attgctcgag aggaattgat
     2341 gtctgaaggg gtgatacagt atgatgacca ttgtagggtt tgtcacaaac ttggggattt
     2401 gctttgctgt gagacatgtt cagcagtata ccatttggaa tgtgtgaagc cacctcttga
     2461 ggaggtgcca gaggacgagt ggcagtgtga agtctgtgta gcacacaagg tgcctggtgt
     2521 gactgactgt gttgctgaaa tccaaaaaaa taaaccatat attcgacatg aacctattgg
     2581 atatgataga agtcggagga aatactggtt cttgaaccga agactcataa tagaagaaga
     2641 tacagaaaat gaaaatgaaa agaaaatttg gtattacagc acaaaggtcc aacttgcaga
     2701 attaattgac tgtctagaca aagattattg ggaagcagaa ctctgcaaaa ttctagaaga
     2761 aatgcgtgaa gaaatccacc gacacatgga cataactgaa gacctgacca ataaggctcg
     2821 gggcagtaac aaatcctttc tggcggcagc taatgaagaa attttggaat ccataagagc
     2881 caaaaaggga gacattgata atgttaaaag cccagaagaa acagaaaaag acaagaatga
     2941 gactgagaat gactctaaag atgctgagaa aaacagagaa gaatttgaag accagtccct
     3001 tgaaaaagac agtgacgaca aaacaccaga tgatgaccct gagcaaggaa aatctgaggt
     3061 aggtgatttc aaatcggaga agtccaacgg ggagctaagt gaatctcctg gagctggaaa
     3121 aggagcatct ggctcaactc gaatcatcac cagattgcgg aatccagata gcaaacttag
     3181 tcagctgaag agccagcagg tggcagccgc tgcacatgaa gcaaataaat tatttaagga
     3241 gggcaaagag gtactggtag ttaactctca aggagaaatt tcacggttga gcaccaaaaa
     3301 ggaagtgatc atgaaaggaa atatcaacaa ttattttaaa ttgggtcaag aagggaagta
     3361 tcgcgtctac cacaatcaat actccaccaa ttcatttgct ttgaataagc accagcacag
     3421 agaagaccat gataagagaa ggcatcttgc acataagttc tgtctgactc cagcaggaga
     3481 gttcaaatgg aacggttctg tccatgggtc caaagttctt accatatcta ctctgagact
     3541 gactatcacc caattagaaa acaacatccc ttcatccttt cttcatccca actgggcatc
     3601 acatagggca aattggatca aggcagttca gatgtgtagc aaacccagag aatttgcatt
     3661 ggctttagcc attttggagt gtgcagttaa accagttgtg atgctaccaa tatggcgaga
     3721 atctttagga cataccaggt tacaccggat gacatcaatt gaaagagaag aaaaggagaa
     3781 agtcaaaaaa aaagagaaga aacaggaaga agaagaaacg atgcagcaag cgacatgggt
     3841 aaaatacaca tttccagtta agcatcaggt ttggaaacaa aaaggtgaag agtacagagt
     3901 gacaggatat ggtggttgga gctggattag taaaactcat gtttataggt ttgttcctaa
     3961 attgccaggc aatactaatg tgaattacag aaagtcgtta gaaggaacca aaaataatat
     4021 ggatgaaaat atggatgagt cagataaaag aaaatgttca cgaagtccaa aaaaaataaa
     4081 aatagagcct gattctgaaa aagatgaggt aaaaggttca gatgctgcaa aaggagcaga
     4141 ccaaaatgaa atggatatct caaagattac tgagaagaag gaccaagatg tgaaggagct
     4201 cttagattct gacagtgata aaccctgcaa ggaagaacca atggaagtag acgatgacat
     4261 gaaaacagag tcacatgtaa attgtcagga gagttctcaa gtagatgtgg tcaatgttag
     4321 tgagggtttt catctaagga ctagttacaa aaagaaaaca aaatcatcca aactagatgg
     4381 acttcttgaa aggagaatta aacagtttac actggaagaa aaacagcgac tcgaaaaaat
     4441 caagttggag ggtggaatta agggtatagg aaagacttct acaaattctt caaaaaatct
     4501 ctctgaatca ccagtaataa cgaaagcaaa agaagggtgt cagagtgact cgatgagaca
     4561 agaacagagc ccaaatgcaa ataatgatca acctgaggac ttgattcagg gatgttcaga
     4621 aagtgattcc tcagttctta gaatgagtga tcctagtcat accacaaaca aactttatcc
     4681 aaaagatcga gtgttagatg atgtctccat tcggagccca gaaacaaaat gtccgaaaca
     4741 aaattccatt gaaaatgaca tagaagaaaa agtctctgac cttgccagta gaggccagga
     4801 acccagtaag agtaaaacaa aaggaaatga ttttttcatc gatgactcta aactagccag
     4861 tgcagatgat attggtactt tgatctgtaa gaacaaaaaa ccgctcatac aggaggaaag
     4921 tgacaccatt gtttcttctt ccaagagtgc tttacattca tcagtgccta aaagtaccaa
     4981 tgacagagat gccacacctc tgtcaagagc aatggacttt gaaggaaaac tgggatgtga
     5041 ctctgaatct aatagcactt tggaaaatag ttctgatacc gtgtctattc aggatagcag
     5101 tgaagaagat atgattgttc agaatagcaa tgaaagcatt tctgaacagt tcagaactcg
     5161 agaacaagat gttgaagtct tggagccgtt aaagtgtgag ttggtttctg gtgagtccac
     5221 tggaaactgt gaggacaggc tgccggtcaa ggggactgaa gcaaatggta aaaaaccaag
     5281 tcagcagaag aaattagagg agagaccagt taataaatgt agtgatcaaa taaagctaaa
     5341 aaataccact gacaaaaaga ataatgaaaa tcgagagtct gaaaagaaag gacagagaac
     5401 aagtacattt caaataaatg gaaaagataa taaacccaaa atatatttga aaggtgaatg
     5461 cttgaaagaa atttctgaga gtagagtagt aagtggtaat gttgaaccaa aggttaataa
     5521 tataaataaa ataatccctg agaatgatat taaatcattg actgttaaag aatctgctat
     5581 aaggccattc attaatggtg atgtcatcat ggaagatttt aatgaaagaa acagctccga
     5641 aacaaaatcg catttgctga gttcttcaga tgctgaaggt aactaccgag atagccttga
     5701 gaccctgcca tcaaccaaag agtctgacag tacacagacg accacaccct cagcatcttg
     5761 tccagaaagc aattcagtta atcaggtaga agatatggaa atagaaacct cagaagttaa
     5821 gaaagttact tcatcaccta ttacttctga agaggaatct aatctcagta atgactttat
     5881 tgatgaaaat ggtctgccca tcaacaaaaa tgaaaatgtc aatggagaat ctaaaagaaa
     5941 aaccgtcatc acagaagtca ccacgatgac ctccacagtg gccacagaat caaaaactgt
     6001 gatcaaggta gaaaaaggcg ataagcaaac tgtggtttct tccacagaaa attgtgcaaa
     6061 atccactgtc acaaccacca ctacaacagt gaccaagctt tccacaccct ccacaggcgg
     6121 cagtgtggac atcatctctg taaaggagca gagcaaaacc gtggtcacca cgacagtgac
     6181 agactccctg accaccacgg gaggcacact ggttacatct atgactgtga gcaaagagta
     6241 ttccacacga gacaaagtga aactgatgaa attttcaaga ccaaagaaga ctcgttcagg
     6301 tacagctctg ccatcctata gaaaatttgt taccaagagc agcaagaaga gcatttttgt
     6361 tttgcctaat gatgacttaa aaaagttggc ccgaaaagga ggaatccgag aggtccctta
     6421 ttttaattac aatgcaaaac ctgctttgga tatatggcca tatccttctc ctagaccgac
     6481 ctttggcatc acttggaggt atagacttca gacagtaaag tccttagctg gagtgagcct
     6541 gatgttacgg ttactgtggg caagtttgag atgggatgat atggcggcca aggctcctcc
     6601 aggaggaggg actacacgga cagaaacatc cgaaactgaa atcacaacaa cagaaataat
     6661 taagaggaga gatgttggtc cttatggcat tcgatctgaa tattgtatca ggaaaatcat
     6721 ttgtcccatt ggagttccag aaacaccaaa agaaacgcct acacctcaga ggaaaggcct
     6781 tcgatcaagt gcactgcggc caaagagacc agaaacgccc aagcaaactg gccctgttat
     6841 tattgaaacc tgggtagcag aagaagaact ggaattgtgg gagatcaggg catttgctga
     6901 gagagtggag aaagaaaagg cacaagcagt tgagcaacag gctaagaaac gactggagca
     6961 gcagaagccg acagtgattg caacttccac tacttcccca acaagcagta caaccagcac
     7021 catctctcca gcacagaagg ttatggtggc ccccataagt ggctcagtta caactggaac
     7081 caaaatggta ctaactacta aagttggatc tccagctaca gtaacattcc aacaaaacaa
     7141 gaactttcat caaacctttg ctacatgggt taagcaaggc cagtcaaatt caggcgttgt
     7201 tcaagtacag cagaaagtcc tgggtatcat tccatcaagt acaggtacca gtcagcaaac
     7261 ctttacttca ttccagccca ggacagcaac agtcacaatt aggcccaata cctcaggctc
     7321 tggaggaacc acaagcaatt cacaagtaat cacagggcct cagattcgcc ctggtatgac
     7381 cgtgattaga acaccactcc aacagtcaac actaggaaag gcaattattc gaacacctgt
     7441 gatggtacag ccaggtgctc ctcagcaagt gatgactcaa atcatcaggg ggcagcctgt
     7501 ctccactgca gtctccgccc ctaacacggt ttcctcaaca cctgggcaga aaagcttaac
     7561 ttcagcaacg tccacttcaa atatacagtc ttcagcctca caaccccctc gcccccaaca
     7621 aggacaagtg aagctcacca tggctcaact tactcagtta acacagggcc acggtggcaa
     7681 tcaaggtttg acagtagtaa ttcaaggaca aggtcaaact actggacagt tgcagttgat
     7741 acctcaaggg gtgactgtac tcccaggccc aggccagcag ctaatgcaag ctgcaatgcc
     7801 aaatggtact gttcagcgat tcctctttac cccattggca acaacagcca ccacagccag
     7861 caccaccacc accactgttt ccacgacagc agcaggtaca ggtgaacaaa ggcagagtaa
     7921 actgtcaccc cagatgcagg tacatcaaga caaaaccctg ccaccagctc agtcatcaag
     7981 tgtgggtcca gcagaagccc agccacagac tgctcagcct tcagctcagc cccagcccca
     8041 aacccagccc cagtccccag ctcagcctga agttcagact cagcctgaag ttcagaccca
     8101 aacaactgtt tcatcccatg tcccttctga agcacaaccc acccacgcac agtcatccaa
     8161 gccccaagtt gcagcacagt ctcagcctca aagtaatgtc caaggacagt ctcctgttcg
     8221 tgtccaaagt ccatcacaga ctcgaatacg tccatcaact ccatcccaac tgtctcctgg
     8281 acaacaatcc caggttcaga ctacaacctc acaaccgatt ccaattcaac cacatacatc
     8341 tcttcagata ccttcccaag gccagccaca gtcacaaccc caggtacagt cttcaactca
     8401 aactctttca tcaggacaaa ctttaaatca agttactgtt tcatccccat cccgtcctca
     8461 gctacaaata cagcagccac agccccaagt cattgctgtg cctcagctgc aacaacaagt
     8521 ccaggttctc tctcagatcc agtcacaggt tgtggctcag atacaggctc agcaaagtgg
     8581 tgtgccccag caaatcaaac tccagttacc tatccaaatt cagcaaagca gtgctgtgca
     8641 gactcaccag attcagaatg tggttacagt gcaggcagcc agtgtgcaag agcagttgca
     8701 aagggttcag caactcaggg atcagcagca aaagaagaaa cagcaacaga tagaaattaa
     8761 gcgtgaacac accctccaag cttctaatca aagtgaaatc attcagaaac aggtggtgat
     8821 gaagcataat gctgtaatag aacatttaaa acagaaaaag agcatgactc cagctgaaag
     8881 agaagagaat caaagaatga ttgtctgtaa ccaggtgatg aagtatattt tggataagat
     8941 agataaagaa gaaaaacagg cagcaaaaaa acggaagcgt gaagagagtg tggagcagaa
     9001 acgtagcaag cagaatgcca ctaagctgtc agctctgctc ttcaagcaca aagagcagct
     9061 cagagccgag atcctgaaga agagagcact cctggacaag gatctgcaaa ttgaagtgca
     9121 ggaagagctg aagagagacc tgaaaattaa gaaagaaaaa gacctgatgc agttggctca
     9181 ggccacagca gtagctgcac cctgcccccc agtgacacca gctcctccag cccctccagc
     9241 ccctccacct tcacctcccc ctccacctgc tgtgcaacac acaggccttc tgtccacgcc
     9301 caccttacct gctgcttccc agaagaggaa gcgggaagag gaaaaagact ccagctcaaa
     9361 gtccaagaaa aagaaaatga tctctactac ctcaaaggaa actaagaagg acacaaagct
     9421 ttactgtatc tgtaaaacgc cttatgatga atctaaattt tatattggct gtgatcggtg
     9481 tcagaattgg taccatgggc gctgcgttgg catcttgcaa agtgaggcag agctcattga
     9541 tgagtatgtc tgtccacagt gccagtcaac agaggatgcc atgacagtgc tcacgccact
     9601 aacagagaag gattatgagg ggttgaagag ggtgctccgt tccttacagg cccataagat
     9661 ggcctggcct ttccttgaac cagtagaccc taatgatgca ccagattatt atggtgttat
     9721 taaggaacct atggaccttg ccaccatgga agaaagagta caaagacgat attatgaaaa
     9781 gctgacggaa tttgtggcag atatgaccaa aatttttgat aactgtcgtt actacaatcc
     9841 aagtgactcc ccattttacc agtgtgcaga agttctcgaa tcattctttg tacagaaatt
     9901 gaaaggcttc aaagctagca ggtctcataa caacaaactg cagtctacag cttcttagta
     9961 atgaactagt ccgtaacttg aaagtatttc gatttcttgg ctttatatat cttgtggaaa
    10021 ggacgagatg ggtcctactg gatgcctcgt acgcgttaag tcgacaatca acctctggat
    10081 tacaaaattt gtgaaagatt gactggtatt cttaactatg ttgctccttt tacgctatgt
    10141 ggatacgctg ctttaatgcc tttgtatcat gctattgctt cccgtatggc tttcattttc
    10201 tcctccttgt ataaatcctg gttgctgtct ctttatgagg agttgtggcc cgttgtcagg
    10261 caacgtggcg tggtgtgcac tgtgtttgct gacgcaaccc ccactggttg gggcattgcc
    10321 accacctgtc agctcctttc cgggactttc gctttccccc tccctattgc cacggcggaa
    10381 ctcatcgccg cctgccttgc ccgctgctgg acaggggctc ggctgttggg cactgacaat
    10441 tccgtggtgt tgtcggggaa atcatcgtcc tttccttggc tgctcgcctg tgttgccacc
    10501 tggattctgc gcgggacgtc cttctgctac gtcccttcgg ccctcaatcc agcggacctt
    10561 ccttcccgcg gcctgctgcc ggctctgcgg cctcttccgc gtcttcgcct tcgccctcag
    10621 acgagtcgga tctccctttg ggccgcctcc ccgcgtcgac tttaagacca atgacttaca
    10681 aggcagctgt agatcttagc cactttttaa aagaaaaggg gggactggaa gggctaattc
    10741 actcccaacg aagacaagat ctgctttttg cttgtactgg gtctctctgg ttagaccaga
    10801 tctgagcctg ggagctctct ggctaactag ggaacccact gcttaagcct caataaagct
    10861 tgccttgagt gcttcaagta gtgtgtgccc gtctgttgtg tgactctggt aactagagat
    10921 ccctcagacc cttttagtca gtgtggaaaa tctctagcag tacgtatagt agttcatgtc
    10981 atcttattat tcagtattta taacttgcaa agaaatgaat atcagagagt gagaggaact
    11041 tgtttattgc agcttataat ggttacaaat aaagcaatag catcacaaat ttcacaaata
    11101 aagcattttt ttcactgcat tctagttgtg gtttgtccaa actcatcaat gtatcttatc
    11161 atgtctggct ctagctatcc cgcccctaac tccgcccatc ccgcccctaa ctccgcccag
    11221 ttccgcccat tctccgcccc atggctgact aatttttttt atttatgcag aggccgaggc
    11281 cgcctcggcc tctgagctat tccagaagta gtgaggaggc ttttttggag gcctagggac
    11341 gtacccaatt cgccctatag tgagtcgtat tacgcgcgct cactggccgt cgttttacaa
    11401 cgtcgtgact gggaaaaccc tggcgttacc caacttaatc gccttgcagc acatccccct
    11461 ttcgccagct ggcgtaatag cgaagaggcc cgcaccgatc gcccttccca acagttgcgc
    11521 agcctgaatg gcgaatggga cgcgccctgt agcggcgcat taagcgcggc gggtgtggtg
    11581 gttacgcgca gcgtgaccgc tacacttgcc agcgccctag cgcccgctcc tttcgctttc
    11641 ttcccttcct ttctcgccac gttcgccggc tttccccgtc aagctctaaa tcgggggctc
    11701 cctttagggt tccgatttag tgctttacgg cacctcgacc ccaaaaaact tgattagggt
    11761 gatggttcac gtagtgggcc atcgccctga tagacggttt ttcgcccttt gacgttggag
    11821 tccacgttct ttaatagtgg actcttgttc caaactggaa caacactcaa ccctatctcg
    11881 gtctattctt ttgatttata agggattttg ccgatttcgg cctattggtt aaaaaatgag
    11941 ctgatttaac aaaaatttaa cgcgaatttt aacaaaatat taacgcttac aatttaggtg
    12001 gcacttttcg gggaaatgtg cgcggaaccc ctatttgttt atttttctaa atacattcaa
    12061 atatgtatcc gctcatgaga caataaccct gataaatgct tcaataatat tgaaaaagga
    12121 agagtatgag tattcaacat ttccgtgtcg cccttattcc cttttttgcg gcattttgcc
    12181 ttcctgtttt tgctcaccca gaaacgctgg tgaaagtaaa agatgctgaa gatcagttgg
    12241 gtgcacgagt gggttacatc gaactggatc tcaacagcgg taagatcctt gagagttttc
    12301 gccccgaaga acgttttcca atgatgagca cttttaaagt tctgctatgt ggcgcggtat
    12361 tatcccgtat tgacgccggg caagagcaac tcggtcgccg catacactat tctcagaatg
    12421 acttggttga gtactcacca gtcacagaaa agcatcttac ggatggcatg acagtaagag
    12481 aattatgcag tgctgccata accatgagtg ataacactgc ggccaactta cttctgacaa
    12541 cgatcggagg accgaaggag ctaaccgctt ttttgcacaa catgggggat catgtaactc
    12601 gccttgatcg ttgggaaccg gagctgaatg aagccatacc aaacgacgag cgtgacacca
    12661 cgatgcctgt agcaatggca acaacgttgc gcaaactatt aactggcgaa ctacttactc
    12721 tagcttcccg gcaacaatta atagactgga tggaggcgga taaagttgca ggaccacttc
    12781 tgcgctcggc ccttccggct ggctggttta ttgctgataa atctggagcc ggtgagcgtg
    12841 ggtctcgcgg tatcattgca gcactggggc cagatggtaa gccctcccgt atcgtagtta
    12901 tctacacgac ggggagtcag gcaactatgg atgaacgaaa tagacagatc gctgagatag
    12961 gtgcctcact gattaagcat tggtaactgt cagaccaagt ttactcatat atactttaga
    13021 ttgatttaaa acttcatttt taatttaaaa ggatctaggt gaagatcctt tttgataatc
    13081 tcatgaccaa aatcccttaa cgtgagtttt cgttccactg agcgtcagac cccgtagaaa
    13141 agatcaaagg atcttcttga gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa
    13201 aaaaaccacc gctaccagcg gtggtttgtt tgccggatca agagctacca actctttttc
    13261 cgaaggtaac tggcttcagc agagcgcaga taccaaatac tgttcttcta gtgtagccgt
    13321 agttaggcca ccacttcaag aactctgtag caccgcctac atacctcgct ctgctaatcc
    13381 tgttaccagt ggctgctgcc agtggcgata agtcgtgtct taccgggttg gactcaagac
    13441 gatagttacc ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc acacagccca
    13501 gcttggagcg aacgacctac accgaactga gatacctaca gcgtgagcta tgagaaagcg
    13561 ccacgcttcc cgaagggaga aaggcggaca ggtatccggt aagcggcagg gtcggaacag
    13621 gagagcgcac gagggagctt ccagggggaa acgcctggta tctttatagt cctgtcgggt
    13681 ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg cggagcctat
    13741 ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc cttttgctgg ccttttgctc
    13801 acatgttctt tcctgcgtta tcccctgatt ctgtggataa ccgtattacc gcctttgagt
    13861 gagctgatac cgctcgccgc agccgaacga ccgagcgcag cgagtcagtg agcgaggaag
    13921 cggaagagcg cccaatacgc aaaccgcctc tccccgcgcg ttggccgatt cattaatgca
    13981 gctggcacga caggtttccc gactggaaag cgggcagtga gcgcaacgca attaatgtga
    14041 gttagctcac tcattaggca ccccaggctt tacactttat gcttccggct cgtatgttgt
    14101 gtggaattgt gagcggataa caatttcaca caggaaacag ctatgaccat gattacgcca
    14161 agcgcgcaat taaccctcac taaagggaac aaaagctgga gctgcaagct taatgtagtc
    14221 ttatgcaata ctcttgtagt cttgcaacat ggtaacgatg agttagcaac atgccttaca
    14281 aggagagaaa aagcaccgtg catgccgatt ggtggaagta aggtggtacg atcgtgcctt
    14341 attaggaagg caacagacgg gtctgacatg gattggacga accactgaat tgccgcattg
    14401 cagagatatt gtatttaagt gcctagctcg atacataaac gggtctctct ggttagacca
    14461 gatctgagcc tgggagctct ctggctaact agggaaccca ctgcttaagc ctcaataaag
    14521 cttgccttga gtgcttcaag tagtgtgtgc ccgtctgttg tgtgactctg gtaactagag
    14581 atccctcaga cccttttagt cagtgtggaa aatctctagc agtggcgccc gaacagggac
    14641 ttgaaagcga aagggaaacc agaggagctc tctcgacgca ggactcggct tgctgaagcg
    14701 cgcacggcaa gaggcgaggg gcggcgactg gtgagtacgc caaaaatttt gactagcgga
    14761 ggctagaagg agagagatgg gtgcgagagc gtcagtatta agcgggggag aattagatcg
    14821 cgatgggaaa aaattcggtt aaggccaggg ggaaagaaaa aatataaatt aaaacatata
    14881 gtatgggcaa gcagggagct agaacgattc gcagttaatc ctggcctgtt agaaacatca
    14941 gaaggctgta gacaaatact gggacagcta caaccatccc ttcagacagg atcagaagaa
    15001 cttagatcat tatataatac agtagcaacc ctctattgtg tgcatcaaag gatagagata
    15061 aaagacacca aggaagcttt agacaagata gaggaagagc aaaacaaaag taagaccacc
    15121 gcacagcaag cggccgctga tcttcagacc tggaggagga gatatgaggg acaattggag
    15181 aagtgaatta tataaatata aagtagtaaa aattgaacca ttaggagtag cacccaccaa
    15241 ggcaaagaga agagtggtgc agagagaaaa aagagcagtg ggaataggag ctttgttcct
    15301 tgggttcttg ggagcagcag gaagcactat gggcgcagcg tcaatgacgc tgacggtaca
    15361 ggccagacaa ttattgtctg gtatagtgca gcagcagaac aatttgctga gggctattga
    15421 ggcgcaacag catctgttgc aactcacagt ctggggcatc aagcagctcc aggcaagaat
    15481 cctggctgtg gaaagatacc taaaggatca acagctcctg gggatttggg gttgctctgg
    15541 aaaactcatt tgcaccactg ctgtgccttg gaatgctagt tggagtaata aatctctgga
    15601 acagatttgg aatcacacga cctggatgga gtgggacaga gaaattaaca attacacaag
    15661 cttaatacac tccttaattg aagaatcgca aaaccagcaa gaaaagaatg aacaagaatt
    15721 attggaatta gataaatggg caagtttgtg gaattggttt aacataacaa attggctgtg
    15781 gtatataaaa ttattcataa tgatagtagg aggcttggta ggtttaagaa tagtttttgc
    15841 tgtactttct atagtgaata gagttaggca gggatattca ccattatcgt ttcagaccca
    15901 cctcccaacc ccgaggggac ccatgcattg catctcaatt agtcagcaac caggtgtgga
    15961 aagtccccag gctccccagc aggcagaagt atgcaaagca tgcgtctcaa ttagtcagca
    16021 accatagtcc cgcccctaac tccgcccatc ccgcccctaa ctccgcccag ttccgcccat
    16081 tctccgcccc atggctgact aatttttttt atttatgcag aggccgaggc cgcctcggcc
    16141 tctgagctat tccagaagta gtgaggaggc ttttttggag gcctaggctt ttgcaaaaag
    16201 ctttctagag gtaccaccgg agcttaccat gaccgagtac aagcccacgg tgcgcctcgc
    16261 cacccgcgac gacgtcccca gggccgtacg caccctcgcc gccgcgttcg ccgactaccc
    16321 cgccacgcgc cacaccgtcg atccggaccg ccacatcgag cgggtcaccg agctgcaaga
    16381 actcttcctc acgcgcgtcg ggctcgacat cggcaaggtg tgggtcgcgg acgacggcgc
    16441 cgccgtggcg gtctggacca cgccggagag cgtcgaagcg ggggcggtgt tcgccgagat
    16501 cggcccgcgc atggccgagt tgagcggttc ccggctggcc gcgcagcaac agatggaagg
    16561 cctcctggcg ccgcaccggc ccaaggagcc cgcgtggttc ctggccaccg tcggcgtctc
    16621 gcccgaccac cagggcaagg gtctgggcag cgccgtcgtg ctccccggag tggaggcggc
    16681 cgagcgcgcc ggggtgcccg ccttcctgga gacctccgcg ccccgcaacc tccccttcta
    16741 cgagcggctc ggcttcaccg tcaccgccga cgtcgaggtg cccgaaggac cgcgcacctg
    16801 gtgcatgacc cgcaagcccg gtgccagtta acgaattcta gatcttgaga caaatggcag
    16861 tattcatcca caattttaaa agaaaagggg ggattggggg gtacagtgca ggggaaagaa
    16921 tagtagacat aatagcaaca gacatacaaa ctaaagaatt acaaaaacaa attacaaaaa
    16981 ttcaaaattt tcgggtttat tacagggaca gcagagatcc actttggcgc cggctcgagg
    17041 cctgcaggtg caaagatgga taaagtttta aacagagagg aatctttgca gctaatggac
    17101 cttctaggtc ttgaaaggag tgggaatt
//
