LOCUS       sequence-353422-        9361 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9361)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 9361)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF3317
FEATURES             Location/Qualifiers
     source          1..9361
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        1..1179
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          232..1170
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     1127..1147
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     protein_bind    1199..1223
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(2115..2139)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             2147..2188
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     misc_feature    2299..2887
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(2352..2372)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(2770..2781)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             2959..3192
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    3270..3391
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(3307..3326)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     3361..3380
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      3431..3566
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(3586..3605)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(3587..3605)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(3615..3632)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(3615..3631)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(3624..3646)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      3773..4228
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(3860..3879)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     4070..4091
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        4254..4358
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(4359..4427,4428..5219)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(4577..4596)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      5390..5978
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     5879..5898
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     6132..6149
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    6266..6287
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(6302..6307,6308..6325,6326..6332)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6340..6356
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     6345..6367
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     6364..6380
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     6364..6380
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     6399..6419
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        6401..6419
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        6447..6673
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             6674..6854
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    6901..7026
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    7519..7752
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             7937..7981
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     promoter        8212..8422
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     promoter        8234..8430
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     rep_origin      8281..8416
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             8462..9064
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGAS"
     primer_bind     complement(8462..8481)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     8958..8978
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     misc_feature    9107..9224
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
ORIGIN
        1 ggctccggtg cccgtcagtg ggcagagcgc acatcgccca cagtccccga gaagttgggg
       61 ggaggggtcg gcaattgaac cggtgcctag agaaggtggc gcggggtaaa ctgggaaagt
      121 gatgtcgtgt actggctccg cctttttccc gagggtgggg gagaaccgta tataagtgca
      181 gtagtcgccg tgaacgttct ttttcgcaac gggtttgccg ccagaacaca ggtaagtgcc
      241 gtgtgtggtt cccgcgggcc tggcctcttt acgggttatg gcccttgcgt gccttgaatt
      301 acttccacct ggctgcagta cgtgattctt gatcccgagc ttcgggttgg aagtgggtgg
      361 gagagttcga ggccttgcgc ttaaggagcc ccttcgcctc gtgcttgagt tgaggcctgg
      421 cctgggcgct ggggccgccg cgtgcgaatc tggtggcacc ttcgcgcctg tctcgctgct
      481 ttcgataagt ctctagccat ttaaaatttt tgatgacctg ctgcgacgct ttttttctgg
      541 caagatagtc ttgtaaatgc gggccaagat ctgcacactg gtatttcggt ttttggggcc
      601 gcgggcggcg acggggcccg tgcgtcccag cgcacatgtt cggcgaggcg gggcctgcga
      661 gcgcggccac cgagaatcgg acgggggtag tctcaagctg gccggcctgc tctggtgcct
      721 ggcctcgcgc cgccgtgtat cgccccgccc tgggcggcaa ggctggcccg gtcggcacca
      781 gttgcgtgag cggaaagatg gccgcttccc ggccctgctg cagggagctc aaaatggagg
      841 acgcggcgct cgggagagcg ggcgggtgag tcacccacac aaaggaaaag ggcctttccg
      901 tcctcagccg tcgcttcatg tgactccacg gagtaccggg cgccgtccag gcacctcgat
      961 tagttctcga gcttttggag tacgtcgtct ttaggttggg gggaggggtt ttatgcgatg
     1021 gagtttcccc acactgagtg ggtggagact gaagttaggc cagcttggca cttgatgtaa
     1081 ttctccttgg aatttgccct ttttgagttt ggatcttggt tcattctcaa gcctcagaca
     1141 gtggttcaaa gtttttttct tccatttcag gtgtcgtgag gctagcatcg attgatcaac
     1201 aagtttgtac aaaaaagttg gcaccatgta tttttcccag gaagaatggg agttattgga
     1261 tcccactcag aaggccctct acaatgatgt aatgcaggaa aactatgaga ctgtcatctc
     1321 tctagcattg tttgtgctcc ccaaacctaa agtgatctcc tgtctagagc aaggggaaga
     1381 gccatgggtt caagtatccc cggagtttaa ggatagtgcc ggaaaatctc ctacagggtt
     1441 aaagctcaaa aacgacactg aaaatcatca gcctgtgtct ctttctgact tagaaataca
     1501 agcatcagca ggcgtcatat caaaaaaggc caaagtaaaa gttccccaga aaacagcagg
     1561 caaagaaaat cattttgata tgcacagagt gggaaaatgg caccaagatt ttccagtgaa
     1621 gaaaagaaag aaactttcaa cctggaaaca agagctgctc aaacttatgg atcgtcacaa
     1681 gaaagattgt gcaagagaga agccttttaa atgtcaggaa tgtgggaaaa ccttcagagt
     1741 tagctctgac cttattaagc accaaagaat tcacactgaa gagaaaccct ataaatgtca
     1801 acagtgtgat aagaggttta gatggagttc agatcttaat aagcacttaa caacacacca
     1861 aggaataaaa ccatataaat gttcatggtg tgggaaaagc ttcagtcaaa atacaaattt
     1921 acatacacac caaagaactc atacaggaga aaagcccttc acatgtcatg aatgtggaaa
     1981 aaaattcagt cagaactccc accttattaa acaccggaga acccacacag gtgagcagcc
     2041 atatacttgt agcatatgca ggagaaactt cagcaggcgg tcaagccttc ttagacacca
     2101 gaaactccac ctgttgccaa ctttcttgta caaagtggtt gatatcggta agcctatccc
     2161 taaccctctc ctcggtctcg attctacgta gtaatgaact agtccgtaac ttgaaagtat
     2221 ttcgatttct tggctttata tatcttgtgg aaaggacgaa ataccatgac caacaataag
     2281 cccacgcgtt aagtcgacaa tcaacctctg gattacaaaa tttgtgaaag attgactggt
     2341 attcttaact atgttgctcc ttttacgcta tgtggatacg ctgctttaat gcctttgtat
     2401 catgctattg cttcccgtat ggctttcatt ttctcctcct tgtataaatc ctggttgctg
     2461 tctctttatg aggagttgtg gcccgttgtc aggcaacgtg gcgtggtgtg cactgtgttt
     2521 gctgacgcaa cccccactgg ttggggcatt gccaccacct gtcagctcct ttccgggact
     2581 ttcgctttcc ccctccctat tgccacggcg gaactcatcg ccgcctgcct tgcccgctgc
     2641 tggacagggg ctcggctgtt gggcactgac aattccgtgg tgttgtcggg gaaatcatcg
     2701 tcctttcctt ggctgctcgc ctgtgttgcc acctggattc tgcgcgggac gtccttctgc
     2761 tacgtccctt cggccctcaa tccagcggac cttccttccc gcggcctgct gccggctctg
     2821 cggcctcttc cgcgtcttcg ccttcgccct cagacgagtc ggatctccct ttgggccgcc
     2881 tccccgcgtc gactttaaga ccaatgactt acaaggcagc tgtagatctt agccactttt
     2941 taaaagaaaa ggggggactg gaagggctaa ttcactccca acgaagacaa gatctgcttt
     3001 ttgcttgtac tgggtctctc tggttagacc agatctgagc ctgggagctc tctggctaac
     3061 tagggaaccc actgcttaag cctcaataaa gcttgccttg agtgcttcaa gtagtgtgtg
     3121 cccgtctgtt gtgtgactct ggtaactaga gatccctcag acccttttag tcagtgtgga
     3181 aaatctctag cagtacgtat agtagttcat gtcatcttat tattcagtat ttataacttg
     3241 caaagaaatg aatatcagag agtgagagga acttgtttat tgcagcttat aatggttaca
     3301 aataaagcaa tagcatcaca aatttcacaa ataaagcatt tttttcactg cattctagtt
     3361 gtggtttgtc caaactcatc aatgtatctt atcatgtctg gctctagcta tcccgcccct
     3421 aactccgccc atcccgcccc taactccgcc cagttccgcc cattctccgc cccatggctg
     3481 actaattttt tttatttatg cagaggccga ggccgcctcg gcctctgagc tattccagaa
     3541 gtagtgagga ggcttttttg gaggcctagg gacgtaccca attcgcccta tagtgagtcg
     3601 tattacgcgc gctcactggc cgtcgtttta caacgtcgtg actgggaaaa ccctggcgtt
     3661 acccaactta atcgccttgc agcacatccc cctttcgcca gctggcgtaa tagcgaagag
     3721 gcccgcaccg atcgcccttc ccaacagttg cgcagcctga atggcgaatg ggacgcgccc
     3781 tgtagcggcg cattaagcgc ggcgggtgtg gtggttacgc gcagcgtgac cgctacactt
     3841 gccagcgccc tagcgcccgc tcctttcgct ttcttccctt cctttctcgc cacgttcgcc
     3901 ggctttcccc gtcaagctct aaatcggggg ctccctttag ggttccgatt tagtgcttta
     3961 cggcacctcg accccaaaaa acttgattag ggtgatggtt cacgtagtgg gccatcgccc
     4021 tgatagacgg tttttcgccc tttgacgttg gagtccacgt tctttaatag tggactcttg
     4081 ttccaaactg gaacaacact caaccctatc tcggtctatt cttttgattt ataagggatt
     4141 ttgccgattt cggcctattg gttaaaaaat gagctgattt aacaaaaatt taacgcgaat
     4201 tttaacaaaa tattaacgct tacaatttag gtggcacttt tcggggaaat gtgcgcggaa
     4261 cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac
     4321 cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg
     4381 tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc
     4441 tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg
     4501 atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga
     4561 gcacttttaa agttctgcta tgtggcgcgg tattatcccg tattgacgcc gggcaagagc
     4621 aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag
     4681 aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga
     4741 gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg
     4801 cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga
     4861 atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgtagcaatg gcaacaacgt
     4921 tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact
     4981 ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt
     5041 ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg
     5101 ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta
     5161 tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac
     5221 tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta
     5281 aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt
     5341 tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt
     5401 tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt
     5461 gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc
     5521 agataccaaa tactgttctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg
     5581 tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg
     5641 ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt
     5701 cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac
     5761 tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg
     5821 acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg
     5881 gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat
     5941 ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt
     6001 tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg
     6061 attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa
     6121 cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcccaata cgcaaaccgc
     6181 ctctccccgc gcgttggccg attcattaat gcagctggca cgacaggttt cccgactgga
     6241 aagcgggcag tgagcgcaac gcaattaatg tgagttagct cactcattag gcaccccagg
     6301 ctttacactt tatgcttccg gctcgtatgt tgtgtggaat tgtgagcgga taacaatttc
     6361 acacaggaaa cagctatgac catgattacg ccaagcgcgc aattaaccct cactaaaggg
     6421 aacaaaagct ggagctgcaa gcttaatgta gtcttatgca atactcttgt agtcttgcaa
     6481 catggtaacg atgagttagc aacatgcctt acaaggagag aaaaagcacc gtgcatgccg
     6541 attggtggaa gtaaggtggt acgatcgtgc cttattagga aggcaacaga cgggtctgac
     6601 atggattgga cgaaccactg aattgccgca ttgcagagat attgtattta agtgcctagc
     6661 tcgatacata aacgggtctc tctggttaga ccagatctga gcctgggagc tctctggcta
     6721 actagggaac ccactgctta agcctcaata aagcttgcct tgagtgcttc aagtagtgtg
     6781 tgcccgtctg ttgtgtgact ctggtaacta gagatccctc agaccctttt agtcagtgtg
     6841 gaaaatctct agcagtggcg cccgaacagg gacttgaaag cgaaagggaa accagaggag
     6901 ctctctcgac gcaggactcg gcttgctgaa gcgcgcacgg caagaggcga ggggcggcga
     6961 ctggtgagta cgccaaaaat tttgactagc ggaggctaga aggagagaga tgggtgcgag
     7021 agcgtcagta ttaagcgggg gagaattaga tcgcgatggg aaaaaattcg gttaaggcca
     7081 gggggaaaga aaaaatataa attaaaacat atagtatggg caagcaggga gctagaacga
     7141 ttcgcagtta atcctggcct gttagaaaca tcagaaggct gtagacaaat actgggacag
     7201 ctacaaccat cccttcagac aggatcagaa gaacttagat cattatataa tacagtagca
     7261 accctctatt gtgtgcatca aaggatagag ataaaagaca ccaaggaagc tttagacaag
     7321 atagaggaag agcaaaacaa aagtaagacc accgcacagc aagcggccgc tgatcttcag
     7381 acctggagga ggagatatga gggacaattg gagaagtgaa ttatataaat ataaagtagt
     7441 aaaaattgaa ccattaggag tagcacccac caaggcaaag agaagagtgg tgcagagaga
     7501 aaaaagagca gtgggaatag gagctttgtt ccttgggttc ttgggagcag caggaagcac
     7561 tatgggcgca gcgtcaatga cgctgacggt acaggccaga caattattgt ctggtatagt
     7621 gcagcagcag aacaatttgc tgagggctat tgaggcgcaa cagcatctgt tgcaactcac
     7681 agtctggggc atcaagcagc tccaggcaag aatcctggct gtggaaagat acctaaagga
     7741 tcaacagctc ctggggattt ggggttgctc tggaaaactc atttgcacca ctgctgtgcc
     7801 ttggaatgct agttggagta ataaatctct ggaacagatt tggaatcaca cgacctggat
     7861 ggagtgggac agagaaatta acaattacac aagcttaata cactccttaa ttgaagaatc
     7921 gcaaaaccag caagaaaaga atgaacaaga attattggaa ttagataaat gggcaagttt
     7981 gtggaattgg tttaacataa caaattggct gtggtatata aaattattca taatgatagt
     8041 aggaggcttg gtaggtttaa gaatagtttt tgctgtactt tctatagtga atagagttag
     8101 gcagggatat tcaccattat cgtttcagac ccacctccca accccgaggg gacccatgca
     8161 ttgcatctca attagtcagc aaccaggtgt ggaaagtccc caggctcccc agcaggcaga
     8221 agtatgcaaa gcatgcgtct caattagtca gcaaccatag tcccgcccct aactccgccc
     8281 atcccgcccc taactccgcc cagttccgcc cattctccgc cccatggctg actaattttt
     8341 tttatttatg cagaggccga ggccgcctcg gcctctgagc tattccagaa gtagtgagga
     8401 ggcttttttg gaggcctagg cttttgcaaa aagctttcta gaggtaccac cggagcttac
     8461 catgaccgag tacaagccca cggtgcgcct cgccacccgc gacgacgtcc ccagggccgt
     8521 acgcaccctc gccgccgcgt tcgccgacta ccccgccacg cgccacaccg tcgatccgga
     8581 ccgccacatc gagcgggtca ccgagctgca agaactcttc ctcacgcgcg tcgggctcga
     8641 catcggcaag gtgtgggtcg cggacgacgg cgccgccgtg gcggtctgga ccacgccgga
     8701 gagcgtcgaa gcgggggcgg tgttcgccga gatcggcccg cgcatggccg agttgagcgg
     8761 ttcccggctg gccgcgcagc aacagatgga aggcctcctg gcgccgcacc ggcccaagga
     8821 gcccgcgtgg ttcctggcca ccgtcggcgt ctcgcccgac caccagggca agggtctggg
     8881 cagcgccgtc gtgctccccg gagtggaggc ggccgagcgc gccggggtgc ccgccttcct
     8941 ggagacctcc gcgccccgca acctcccctt ctacgagcgg ctcggcttca ccgtcaccgc
     9001 cgacgtcgag gtgcccgaag gaccgcgcac ctggtgcatg acccgcaagc ccggtgccag
     9061 ttaacgaatt ctagatcttg agacaaatgg cagtattcat ccacaatttt aaaagaaaag
     9121 gggggattgg ggggtacagt gcaggggaaa gaatagtaga cataatagca acagacatac
     9181 aaactaaaga attacaaaaa caaattacaa aaattcaaaa ttttcgggtt tattacaggg
     9241 acagcagaga tccactttgg cgccggctcg aggcctgcag gtgcaaagat ggataaagtt
     9301 ttaaacagag aggaatcttt gcagctaatg gaccttctag gtcttgaaag gagtgggaat
     9361 t
//
