LOCUS       sequence-353423-        9582 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9582)
  AUTHORS   Levy C, Khaled M, Iliopoulos D, Janas MM, Schubert S, Pinner S, Chen
            PH, Li S, Fletcher AL, Yokoyama S, Scott KL, Garraway LA, Song JS, 
            Granter SR, Turley SJ, Fisher DE, Novina CD
  TITLE     Intronic miR-211 assumes the tumor suppressive function of its host 
            gene in melanoma.
  JOURNAL   Mol Cell. 2010 Dec 10. 40(5):841-9.
  PUBMED    21109473
REFERENCE   2  (bases 1 to 9582)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Cell. 
            2010 Dec 10. 40(5):841-9."
COMMENT     Sequence Label: PLKO-mcherry-luc-puro-miR-211
FEATURES             Location/Qualifiers
     source          1..9582
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        1..249
                     /label=U6 promoter
                     /note="RNA polymerase III promoter for human U6 snRNA 
                     (Domitrovich & Kunkel, 2003)"
     primer_bind     1..21
                     /label=hU6-F
                     /note="Human U6 promoter, forward primer"
     primer_bind     172..191
                     /label=LKO.1 5'
                     /note="Human U6 promoter, forward primer"
     misc_feature    408..525
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        574..1084
                     /label=hPGK promoter
                     /note="human phosphoglycerate kinase 1 promoter"
     primer_bind     1003..1021
                     /label=hPGK-F
                     /note="Human PGK promoter, forward primer"
     regulatory      1093..1102
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             2758..2817
                     /codon_start=1
                     /product="2A peptide from equine rhinitis A virus 
                     polyprotein"
                     /label=E2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="QCTNYALLKLAGDVESNPGP"
     primer_bind     complement(2969..2987)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     CDS             3547..3600
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     LTR             4278..4511
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    4583..4704
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(4620..4639)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     4674..4693
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      4744..4879
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(4899..4918)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(4900..4918)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(4928..4945)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(4928..4944)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(4937..4959)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      5086..5541
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(5173..5192)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     5383..5404
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        5567..5671
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(5672..5740,5741..6532)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(5890..5909)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      6703..7291
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     7192..7211
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     7445..7462
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    7579..7600
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(7615..7620,7621..7638,7639..7645)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7653..7669
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     7658..7680
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     7677..7693
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     7677..7693
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     7712..7732
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        7714..7732
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        7760..7986
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             7987..8167
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    8214..8339
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    8832..9065
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
ORIGIN
        1 gagggcctat ttcccatgat tccttcatat ttgcatatac gatacaaggc tgttagagag
       61 ataattggaa ttaatttgac tgtaaacaca aagatattag tacaaaatac gtgacgtaga
      121 aagtaataat ttcttgggta gtttgcagtt ttaaaattat gttttaaaat ggactatcat
      181 atgcttaccg taacttgaaa gtatttcgat ttcttggctt tatatatctt gtggaaagga
      241 cgaaacaccg gtcacctggc catgtgactt gtgggcttcc ctttgtcatc cttcgcctag
      301 ggctctgagc agggcaggga cagcaaaggg gtgctcagtt gtcacttccc acagcacgga
      361 gtttttgaat tctcgacctc gagacaaatg gcagtattca tccacaattt taaaagaaaa
      421 ggggggattg gggggtacag tgcaggggaa agaatagtag acataatagc aacagacata
      481 caaactaaag aattacaaaa acaaattaca aaaattcaaa attttcgggt ttattacagg
      541 gacagcagag atccactttg gccgcggctc gagggggttg gggttgcgcc ttttccaagg
      601 cagccctggg tttgcgcagg gacgcggctg ctctgggcgt ggttccggga aacgcagcgg
      661 cgccgaccct gggtctcgca cattcttcac gtccgttcgc agcgtcaccc ggatcttcgc
      721 cgctaccctt gtgggccccc cggcgacgct tcctgctccg cccctaagtc gggaaggttc
      781 cttgcggttc gcggcgtgcc ggacgtgaca aacggaagcc gcacgtctca ctagtaccct
      841 cgcagacgga cagcgccagg gagcaatggc agcgcgccga ccgcgatggg ctgtggccaa
      901 tagcggctgc tcagcagggc gcgccgagag cagcggccgg gaaggggcgg tgcgggaggc
      961 ggggtgtggg gcggtagtgt gggccctgtt cctgcccgcg cggtgttccg cattctgcaa
     1021 gcctccggag cgcacgtcgg cagtcggctc cctcgttgac cgaatcaccg acctctctcc
     1081 ccagggggat ccgccaccat ggaagatgcc aagaacatca agaaaggccc tgcccccttc
     1141 taccccctgg aagatggcac agccggcgag cagctgcaca aggccatgaa gagatacgcc
     1201 ctcgtccctg gaacaatcgc cttcaccgac gcccacatcg aggtggacat cacctacgcc
     1261 gagtacttcg agatgagcgt gcggctggcc gaggccatga aacgctacgg cctgaacacc
     1321 aaccaccgga tcgtggtgtg cagcgagaac agcctgcagt tcttcatgcc cgtgctgggc
     1381 gccctgttca tcggcgtggc cgtggcccct gccaacgaca tctacaacga gcgggagctg
     1441 ctgaacagca tgggcatcag ccagcccacc gtggtgttcg tgagcaagaa gggcctgcag
     1501 aaaatcctga acgtgcagaa gaagctgccc atcatccaga aaatcatcat catggacagc
     1561 aagaccgact accagggctt ccagagcatg tacaccttcg tgaccagcca cctgccccct
     1621 ggcttcaacg agtacgactt cgtgcccgag agcttcgaca gggacaagac catcgccctg
     1681 atcatgaaca gcagcggcag caccggcctg cctaaaggcg tggccctgcc tcaccggacc
     1741 gcctgcgtgc ggttcagcca cgcccgggac cccatcttcg gcaaccagat catccccgac
     1801 accgccatcc tgagcgtggt gcccttccac cacggcttcg gcatgttcac caccctgggc
     1861 tacctgatct gcggcttccg ggtggtgctg atgtatcggt tcgaggaaga gctgttcctg
     1921 cggagcctgc aggactacaa gatccagagc gccctgctgg tgcccaccct gttcagcttt
     1981 ttcgccaaga gcaccctgat cgacaagtac gacctgagca acctgcacga gatcgccagc
     2041 ggcggagccc ccctgagcaa agaagtgggc gaagccgtcg ccaagcggtt ccacctgccc
     2101 ggcatccggc agggctatgg cctgaccgag accacaagcg ccattctgat cacccccgag
     2161 ggcgacgaca agcctggcgc cgtgggcaag gtggtgcctt tcttcgaggc caaggtggtg
     2221 gacctggaca ccggcaagac cctgggcgtg aaccagcggg gcgagctgtg cgtgaggggc
     2281 cccatgatca tgagcggcta cgtgaacaac cccgaggcca ccaacgccct gattgacaag
     2341 gacggctggc tgcacagcgg cgacatcgcc tactgggacg aggacgagca cttcttcatc
     2401 gtggaccggc tgaagagcct gatcaagtac aagggctacc aggtggcccc agccgagctg
     2461 gaaagcatcc tgctgcagca ccccaacatc ttcgacgccg gggtggccgg cctgcccgac
     2521 gatgacgccg gcgagctgcc tgccgccgtg gtggtcctcg aacatggcaa aaccatgacc
     2581 gagaaagaaa tcgtggacta cgtggccagc caggtgacca ccgccaagaa gctgagaggc
     2641 ggcgtcgtgt ttgtggacga ggtgcccaag ggcctgaccg gcaagctgga cgcccggaag
     2701 atccgggaga tcctgattaa ggccaagaag ggcggcaaga tcgccgtggg ctccggccag
     2761 tgcaccaact acgccctgct gaagctggcc ggcgacgtgg agagcaaccc tggccctttc
     2821 gaaatggtgt ccaagggcga agaggacaac atggccatca tcaaagagtt tatgcggttc
     2881 aaggtgcaca tggaaggcag cgtgaacggc cacgagttcg agatcgaggg cgagggcgaa
     2941 ggcaggccct acgagggcac ccagaccgcc aagctgaagg tgaccaaggg cggacctctg
     3001 cccttcgcct gggacatcct gagcccccag ttcatgtacg gcagcaaggc ctacgtgaag
     3061 caccccgccg acatccccga ctacctgaag ctgtccttcc ccgagggctt caagtgggag
     3121 cgggtgatga acttcgagga cggcggggtg gtgaccgtga cccaggatag ctccctgcag
     3181 gacggcgagt tcatctacaa ggtgaaactg cggggcacca acttccccag cgacggcccc
     3241 gtgatgcaga aaaagacaat gggctgggag gccagcagcg agcggatgta ccccgaggat
     3301 ggcgccctga agggcgagat caagcagcgg ctgaagctga aggacggcgg ccactacgac
     3361 gccgaggtga aaaccaccta caaggccaaa aagcccgtgc agctgcctgg cgcctacaac
     3421 gtgaacatca agctggatat caccagccac aacgaggact acaccatcgt ggagcagtac
     3481 gagcgggccg agggcagaca cagcaccggc ggcatggacg agctgtacaa gctcagcggc
     3541 agcggcgagg gcagaggcag cctgctgacc tgcggcgatg tggaggaaaa cccaggccca
     3601 atgaccgagt acaagcctac cgtgaggctg gccacccggg acgacgtgcc cagagccgtg
     3661 aggacactgg ccgccgcctt cgccgactac cccgccaccc ggcacaccgt ggaccccgac
     3721 cggcacatcg agcgggtgac cgagctgcag gaactgttcc tgaccagagt gggcctggat
     3781 atcggcaaag tgtgggtggc cgacgacgga gccgccgtgg ccgtgtggac cacccccgag
     3841 tccgtggagg ccggagccgt gtttgccgag atcggcccca ggatggccga gctgtccggc
     3901 agcaggctgg ccgcccagca gcagatggaa ggcctgctgg cccctcaccg gcccaaagag
     3961 cccgcctggt tcctggccac cgtgggcgtg agccccgacc accagggcaa gggcctgggc
     4021 agcgccgtgg tgctgccagg cgtggaagcc gccgagaggg ccggagtgcc cgccttcctg
     4081 gaaaccagcg cccccaggaa cctgcccttc tacgagcggc tgggctttac cgtgaccgcc
     4141 gacgtggagg tgccagaggg ccccaggacc tggtgcatga cccggaagcc aggcgcctga
     4201 gaattgggta cctttaagac caatgactta caaggcagct gtagatctta gccacttttt
     4261 aaaagaaaag gggggactgg aagggctaat tcactcccaa cgaagacaag atctgctttt
     4321 tgcttgtact gggtctctct ggttagacca gatctgagcc tgggagctct ctggctaact
     4381 agggaaccca ctgcttaagc ctcaataaag cttgccttga gtgcttcaag tagtgtgtgc
     4441 ccgtctgttg tgtgactctg gtaactagag atccctcaga cccttttagt cagtgtggaa
     4501 aatctctagc agtagtagtt catgtcatct tattattcag tatttataac ttgcaaagaa
     4561 atgaatatca gagagtgaga ggaacttgtt tattgcagct tataatggtt acaaataaag
     4621 caatagcatc acaaatttca caaataaagc atttttttca ctgcattcta gttgtggttt
     4681 gtccaaactc atcaatgtat cttatcatgt ctggctctag ctatcccgcc cctaactccg
     4741 cccatcccgc ccctaactcc gcccagttcc gcccattctc cgccccatgg ctgactaatt
     4801 ttttttattt atgcagaggc cgaggccgcc tcggcctctg agctattcca gaagtagtga
     4861 ggaggctttt ttggaggcct agggacgtac ccaattcgcc ctatagtgag tcgtattacg
     4921 cgcgctcact ggccgtcgtt ttacaacgtc gtgactggga aaaccctggc gttacccaac
     4981 ttaatcgcct tgcagcacat ccccctttcg ccagctggcg taatagcgaa gaggcccgca
     5041 ccgatcgccc ttcccaacag ttgcgcagcc tgaatggcga atgggacgcg ccctgtagcg
     5101 gcgcattaag cgcggcgggt gtggtggtta cgcgcagcgt gaccgctaca cttgccagcg
     5161 ccctagcgcc cgctcctttc gctttcttcc cttcctttct cgccacgttc gccggctttc
     5221 cccgtcaagc tctaaatcgg gggctccctt tagggttccg atttagtgct ttacggcacc
     5281 tcgaccccaa aaaacttgat tagggtgatg gttcacgtag tgggccatcg ccctgataga
     5341 cggtttttcg ccctttgacg ttggagtcca cgttctttaa tagtggactc ttgttccaaa
     5401 ctggaacaac actcaaccct atctcggtct attcttttga tttataaggg attttgccga
     5461 tttcggccta ttggttaaaa aatgagctga tttaacaaaa atttaacgcg aattttaaca
     5521 aaatattaac gcttacaatt taggtggcac ttttcgggga aatgtgcgcg gaacccctat
     5581 ttgtttattt ttctaaatac attcaaatat gtatccgctc atgagacaat aaccctgata
     5641 aatgcttcaa taatattgaa aaaggaagag tatgagtatt caacatttcc gtgtcgccct
     5701 tattcccttt tttgcggcat tttgccttcc tgtttttgct cacccagaaa cgctggtgaa
     5761 agtaaaagat gctgaagatc agttgggtgc acgagtgggt tacatcgaac tggatctcaa
     5821 cagcggtaag atccttgaga gttttcgccc cgaagaacgt tttccaatga tgagcacttt
     5881 taaagttctg ctatgtggcg cggtattatc ccgtattgac gccgggcaag agcaactcgg
     5941 tcgccgcata cactattctc agaatgactt ggttgagtac tcaccagtca cagaaaagca
     6001 tcttacggat ggcatgacag taagagaatt atgcagtgct gccataacca tgagtgataa
     6061 cactgcggcc aacttacttc tgacaacgat cggaggaccg aaggagctaa ccgctttttt
     6121 gcacaacatg ggggatcatg taactcgcct tgatcgttgg gaaccggagc tgaatgaagc
     6181 cataccaaac gacgagcgtg acaccacgat gcctgtagca atggcaacaa cgttgcgcaa
     6241 actattaact ggcgaactac ttactctagc ttcccggcaa caattaatag actggatgga
     6301 ggcggataaa gttgcaggac cacttctgcg ctcggccctt ccggctggct ggtttattgc
     6361 tgataaatct ggagccggtg agcgtgggtc tcgcggtatc attgcagcac tggggccaga
     6421 tggtaagccc tcccgtatcg tagttatcta cacgacgggg agtcaggcaa ctatggatga
     6481 acgaaataga cagatcgctg agataggtgc ctcactgatt aagcattggt aactgtcaga
     6541 ccaagtttac tcatatatac tttagattga tttaaaactt catttttaat ttaaaaggat
     6601 ctaggtgaag atcctttttg ataatctcat gaccaaaatc ccttaacgtg agttttcgtt
     6661 ccactgagcg tcagaccccg tagaaaagat caaaggatct tcttgagatc ctttttttct
     6721 gcgcgtaatc tgctgcttgc aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc
     6781 ggatcaagag ctaccaactc tttttccgaa ggtaactggc ttcagcagag cgcagatacc
     6841 aaatactgtt cttctagtgt agccgtagtt aggccaccac ttcaagaact ctgtagcacc
     6901 gcctacatac ctcgctctgc taatcctgtt accagtggct gctgccagtg gcgataagtc
     6961 gtgtcttacc gggttggact caagacgata gttaccggat aaggcgcagc ggtcgggctg
     7021 aacggggggt tcgtgcacac agcccagctt ggagcgaacg acctacaccg aactgagata
     7081 cctacagcgt gagctatgag aaagcgccac gcttcccgaa gggagaaagg cggacaggta
     7141 tccggtaagc ggcagggtcg gaacaggaga gcgcacgagg gagcttccag ggggaaacgc
     7201 ctggtatctt tatagtcctg tcgggtttcg ccacctctga cttgagcgtc gatttttgtg
     7261 atgctcgtca ggggggcgga gcctatggaa aaacgccagc aacgcggcct ttttacggtt
     7321 cctggccttt tgctggcctt ttgctcacat gttctttcct gcgttatccc ctgattctgt
     7381 ggataaccgt attaccgcct ttgagtgagc tgataccgct cgccgcagcc gaacgaccga
     7441 gcgcagcgag tcagtgagcg aggaagcgga agagcgccca atacgcaaac cgcctctccc
     7501 cgcgcgttgg ccgattcatt aatgcagctg gcacgacagg tttcccgact ggaaagcggg
     7561 cagtgagcgc aacgcaatta atgtgagtta gctcactcat taggcacccc aggctttaca
     7621 ctttatgctt ccggctcgta tgttgtgtgg aattgtgagc ggataacaat ttcacacagg
     7681 aaacagctat gaccatgatt acgccaagcg cgcaattaac cctcactaaa gggaacaaaa
     7741 gctggagctg caagcttaat gtagtcttat gcaatactct tgtagtcttg caacatggta
     7801 acgatgagtt agcaacatgc cttacaagga gagaaaaagc accgtgcatg ccgattggtg
     7861 gaagtaaggt ggtacgatcg tgccttatta ggaaggcaac agacgggtct gacatggatt
     7921 ggacgaacca ctgaattgcc gcattgcaga gatattgtat ttaagtgcct agctcgatac
     7981 ataaacgggt ctctctggtt agaccagatc tgagcctggg agctctctgg ctaactaggg
     8041 aacccactgc ttaagcctca ataaagcttg ccttgagtgc ttcaagtagt gtgtgcccgt
     8101 ctgttgtgtg actctggtaa ctagagatcc ctcagaccct tttagtcagt gtggaaaatc
     8161 tctagcagtg gcgcccgaac agggacttga aagcgaaagg gaaaccagag gagctctctc
     8221 gacgcaggac tcggcttgct gaagcgcgca cggcaagagg cgaggggcgg cgactggtga
     8281 gtacgccaaa aattttgact agcggaggct agaaggagag agatgggtgc gagagcgtca
     8341 gtattaagcg ggggagaatt agatcgcgat gggaaaaaat tcggttaagg ccagggggaa
     8401 agaaaaaata taaattaaaa catatagtat gggcaagcag ggagctagaa cgattcgcag
     8461 ttaatcctgg cctgttagaa acatcagaag gctgtagaca aatactggga cagctacaac
     8521 catcccttca gacaggatca gaagaactta gatcattata taatacagta gcaaccctct
     8581 attgtgtgca tcaaaggata gagataaaag acaccaagga agctttagac aagatagagg
     8641 aagagcaaaa caaaagtaag accaccgcac agcaagcggc cgctgatctt cagacctgga
     8701 ggaggagata tgagggacaa ttggagaagt gaattatata aatataaagt agtaaaaatt
     8761 gaaccattag gagtagcacc caccaaggca aagagaagag tggtgcagag agaaaaaaga
     8821 gcagtgggaa taggagcttt gttccttggg ttcttgggag cagcaggaag cactatgggc
     8881 gcagcgtcaa tgacgctgac ggtacaggcc agacaattat tgtctggtat agtgcagcag
     8941 cagaacaatt tgctgagggc tattgaggcg caacagcatc tgttgcaact cacagtctgg
     9001 ggcatcaagc agctccaggc aagaatcctg gctgtggaaa gatacctaaa ggatcaacag
     9061 ctcctgggga tttggggttg ctctggaaaa ctcatttgca ccactgctgt gccttggaat
     9121 gctagttgga gtaataaatc tctggaacag atttggaatc acacgacctg gatggagtgg
     9181 gacagagaaa ttaacaatta cacaagctta atacactcct taattgaaga atcgcaaaac
     9241 cagcaagaaa agaatgaaca agaattagat aaatgggcaa gtttgtggaa ttggtttaac
     9301 ataacaaatt ggctgtggta tataaaatta ttcataatga tagtaggagg cttggtaggt
     9361 ttaagaatag tttttgctgt actttctata gtgaatagag ttaggcaggg atattcacca
     9421 ttatcgtttc agacccacct cccaaccccg aggggacccg acaggcccga aggaatagaa
     9481 gaagaaggtg gagagagaga cagagacaga tccattcgat tagtgaacgg atctcgacgg
     9541 tatcgatcac gagactagcc tcgagcggcc gcccccttca cc
//
